Identification of the Y chromosome in chinook salmon (Oncorhynchus tshawytscha)

2001 ◽  
Vol 92 (1-2) ◽  
pp. 108-110 ◽  
Author(s):  
J. Stein ◽  
R.B. Phillips ◽  
R.H. Devlin
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha

scholarly journals Heritability and Y-chromosome influence in the jack male life history of chinook salmon (Oncorhynchus tshawytscha)

Heredity ◽  
2002 ◽  
Vol 89 (4) ◽  
pp. 311-317 ◽  
Author(s):  
D D Heath ◽  
L Rankin ◽  
C A Bryden ◽  
J W Heath ◽  
J M Shrimpton
Keyword(s):  
Life History ◽  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
History Of ◽  
Male Life History

Isolation of a Y-Chromosomal DNA Probe Capable of Determining Genetic Sex in Chinook Salmon (Oncorhynchus tshawytscha)

1991 ◽  
Vol 48 (9) ◽  
pp. 1606-1612 ◽  
Author(s):  
Robert H. Devlin ◽  
B. Kelly McNeil ◽  
T. David D. Groves ◽  
Edward M. Donaldson
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Subtractive Hybridization ◽  
Specific Pattern ◽  
Dna Probe ◽  
Chromosomal Dna ◽  
Males And Females ◽  
Dna Fragment ◽  
Male Specific

We have used subtractive hybridization methodologies to selectively clone a DNA fragment from the Y chromosome of chinook salmon (Oncorhynchus tshawytscha). This clone specifically detects a single 8-kb Bam HI restriction fragment in Southern blots of male genomic DNA that is absent in females. This sex-specific pattern was observed in over 50 individuals from five separate chinook stocks, suggesting that the organization of this sequence is conserved in this species. The strength of the hybridization signal, and the pattern observed in multiple enzyme digests, indicates that this sequence is repeated in the male chinook genome. With long autoradiographic exposures, fainter additional sequences can also be detected both in males and females, suggesting that other similar sequences not organized in a sex-specific fashion are found on the X chromosome and/or autosomes. Examination of the segregation of this DNA sequence in families showed that the male-specific pattern segregates from father to son, providing strong evidence that the DNA probe is from the Y chromosome. The application of Y-chromosomal probes to the commercial culture of this species will simplify the synthesis of new monosex strains and allow verification of existing genotypic female, phenotypic male stocks.

Variation of Y-chromosome DNA markers in Chinook salmon (Oncorhynchus tshawytscha) populations

2005 ◽  
Vol 62 (6) ◽  
pp. 1386-1399 ◽  
Author(s):  
R H Devlin ◽  
L Park ◽  
D M Sakhrani ◽  
J D Baker ◽  
A R Marshall ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Dna Markers ◽  
Oncorhynchus Tshawytscha ◽  
Chromosome Structure ◽  
Single Copy ◽  
Structural Variants ◽  
Sex Marker ◽  
High Incidence ◽  
Normal Males

Two Y-chromosome DNA markers (a repetitive sequence, OtY1, and a single-copy marker, GH-Y) tightly linked to the sex-determination locus have been examined for their association with sexual development among 55 populations of Chinook salmon (Oncorhynchus tshawytscha) from the Yukon, British Columbia, Washington, Oregon, and Idaho. Normal linkage has been observed in 96.7% of 2478 individuals examined. Only five males (0.44%) were found lacking both markers (none from Canadian systems), and 14 females (1.04%) from US populations and two females (0.15%) from Canadian populations were found to possess both markers. Variants identified included weakly amplifying alleles for GH-Y and OtY1 and structural variants identified by Southern-blot analysis. The frequency of variants in males was more than 2-fold that in females, and males deficient in GH-Y were more common (3.6%) than males deficient in the repetitive OtY1 sequence (0.7%). Some individuals (of both sexes) possessed fewer copies of the OtY1 repeat than normal males, revealing molecular dynamics that alter Y-chromosome structure within and among populations. A population (Hanford Reach) previously reported as having a high incidence of females possessing the OtY1 marker, and suspected of being sex-reversed, was found to have normal sex-marker genotypes in the present study.

Corrigendum: Variation of Y-chromosome DNA markers in Chinook salmon (Oncorhynchus tshawytscha) populations

2016 ◽  
Vol 73 (1) ◽  
pp. 153-153
Author(s):  
R.H. Devlin ◽  
L. Park ◽  
D.M. Sakhrani ◽  
J.D. Baker ◽  
A.R. Marshall ◽  
...  
Keyword(s):  
Y Chromosome ◽  
Chinook Salmon ◽  
Dna Markers ◽  
Oncorhynchus Tshawytscha

Y-specific sequences and polymorphisms in rainbow trout and Chinook salmon

Genome ◽  
2008 ◽  
Vol 51 (9) ◽  
pp. 739-748 ◽  
Author(s):  
Joseph P. Brunelli ◽  
Kelsey J. Wertzler ◽  
Kyle Sundin ◽  
Gary H. Thorgaard
Keyword(s):  
Rainbow Trout ◽  
Y Chromosome ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Aflp Analysis ◽  
Specific Sequence ◽  
Chromosome Marker ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Improved Methods

Improved methods for genetically sexing salmonids and for characterization of Y-chromosome homologies between species can contribute to understanding the evolution of sex chromosomes and sex-determining mechanisms. In this study we have explored 12.5 kb of Y-chromosome-specific sequence flanking the previously described OtY2 locus in Chinook salmon ( Oncorhynchus tshawytscha ) and 21 kb of homologous rainbow trout ( Oncorhynchus mykiss ) Y-chromosome-specific sequence. This is the first confirmed Y-specific sequence for rainbow trout. New Y-specific markers are described for Chinook salmon (OtY3) and rainbow trout (OmyY1), which are readily detected by PCR assays and are advantageous because they also produce autosomal control amplification products. Additionally, AFLP analysis of Chinook salmon yielded another potential Y-chromosome marker. These descriptions will facilitate genotypic sexing and should be useful for population studies of Y-chromosome polymorphisms and for future studies to characterize what appears to be a common sex-determining mechanism between these species.

Systematic hexamitid (Protozoa: Diplomonadida) Infection in seawater pen-reared Chinook salmon Oncorhynchus tshawytscha

1992 ◽  
Vol 14 ◽  
pp. 81-89 ◽  
Author(s):  
ML Kent ◽  
J Ellis ◽  
JW Fournie ◽  
SC Dawe ◽  
JW Bagshaw ◽  
...  
Keyword(s):  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha

scholarly journals Managed Wetlands Can Benefit Juvenile Chinook Salmon in a Tidal Marsh

2021 ◽  
Author(s):  
Nicole M. Aha ◽  
Peter B. Moyle ◽  
Nann A. Fangue ◽  
Andrew L. Rypel ◽  
John R. Durand
Keyword(s):  
San Francisco ◽  
Growth Rates ◽  
Chinook Salmon ◽  
Oncorhynchus Tshawytscha ◽  
Nursery Habitat ◽  
San Francisco Estuary ◽  
Rice Fields ◽  
Fish Growth ◽  
Juvenile Chinook Salmon ◽  
Juvenile Chinook

AbstractLoss of estuarine and coastal habitats worldwide has reduced nursery habitat and function for diverse fishes, including juvenile Chinook salmon (Oncorhynchus tshawytscha). Underutilized off-channel habitats such as flooded rice fields and managed ponds present opportunities for improving rearing conditions and increasing habitat diversity along migratory corridors. While experiments in rice fields have shown enhanced growth rates of juvenile fishes, managed ponds are less studied. To evaluate the potential of these ponds as a nursery habitat, juvenile Chinook salmon (~ 2.8 g, 63 mm FL) were reared in cages in four contrasting locations within Suisun Marsh, a large wetland in the San Francisco Estuary. The locations included a natural tidal slough, a leveed tidal slough, and the inlet and outlet of a tidally muted managed pond established for waterfowl hunting. Fish growth rates differed significantly among locations, with the fastest growth occurring near the outlet in the managed pond. High zooplankton biomass at the managed pond outlet was the best correlate of salmon growth. Water temperatures in the managed pond were also cooler and less variable compared to sloughs, reducing thermal stress. The stress of low dissolved oxygen concentrations within the managed pond was likely mediated by high concentrations of zooplankton and favorable temperatures. Our findings suggest that muted tidal habitats in the San Francisco Estuary and elsewhere could be managed to promote growth and survival of juvenile salmon and other native fishes.

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