scholarly journals Modulatory Role of Vitamin E on Proton Pump (ATPase) Activity of Cadmium Chloride-Induced Testicular Damage in Wistar Rats

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Olugbemi T. Olaniyan ◽  
Abdulfatai O. Ojewale ◽  
Olugbenga O. Eweoya ◽  
Adetola A. Adedoyin ◽  
Olamide A. Adesanya ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Single Dose ◽  
Vitamin E ◽  
Proton Pump ◽  
Cadmium Chloride ◽  
Wistar Rats ◽  
Sperm Count ◽  
Testicular Damage ◽  
Pump Activity

Proton pumps are membrane-bound enzymes important in generating gradients that help in maintaining cellular ion homeostasis, cell membrane potential, water, and solute transport across the cell surface. This study investigated the modulatory role of vitamin E on proton pump activity and reproductive parameters in cadmium-induced testicular damage. Twenty (20) male Wistar rats weighing between 180 and 200 g were sorted into 4 groups of five rats each. Group I served as the control and was given normal saline orally, Group II rats were treated with a single dose of 2 mg/kg BW cadmium chloride (CdCl2) intraperitoneally, Group III rats were given 100 mg/kg BW of vitamin E orally, and Group IV rats were given 100 mg/kg BW of vitamin E orally for 30 days prior to intraperitoneal administration of single dose of 2 mg/kg BW of cadmium chloride. The rats were anaesthetized with diethyl ether, and blood samples were obtained for sex hormonal analysis; caudal epididymis was dissected for sperm count, motility, and viability, and the testis were homogenized for lipid peroxidation and proton pump (Na+/K+ ATPase, Ca2+ ATPase, and Mg2+ ATPase) activity. Proton pump activity was assayed spectrophotometrically using the Stewart method to determine the inorganic phosphate level. Histopathological changes of the testis were also studied. The group treated with CdCl2 showed a significant ( p < 0.05 ) decrease in proton pump activity, sperm count, and motility and a significant ( p < 0.05 ) increase in malondialdehyde level when compared with the control group. The CdCl2-treated group also showed decrease reproductive organ weights and hormonal levels and cause necrosis of spermatogonia lining the seminiferous tubules. Rats treated with vitamin E orally for 30 days prior to CdCl2 exposure showed improvement in proton pump activity, a significant ( p < 0.05 ) increase in sperm parameters and luteinizing hormonal level, and a decrease in the lipid peroxidation level as compared with the CdCl2 group. This study showed that vitamin E ameliorated the toxic effect of CdCl2 on proton pump activity in the testes, hence improving testicular integrity, structures, and functions.

Aqueous extract of Adansonia digitata prevents Cadmium Chloride-induced testicular damage in Wistar Rats

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Babatunde Joseph Dare ◽  
Olugbemi Tope Olaniyan ◽  
Oluwafemi Isaac Oyeniyi ◽  
Gloria Enevwo Okotie ◽  
Ismail Adetayo Lawal ◽  
...  
Keyword(s):  
Single Dose ◽  
Vitamin E ◽  
Aqueous Extract ◽  
Cadmium Chloride ◽  
Wistar Rats ◽  
Antioxidant Activities ◽  
Sucrose Solution ◽  
Saline Group ◽  
Testicular Damage ◽  
Adansonia Digitata

Abstract Objectives The role of aqueous extract of Adansonia digitata was investigated against cadmium chloride-induced testicular damage in Wistar Rats. Methods Thirty (30) male Wistar Rats weighing (150–170) were divided into six groups (n=5). Group A served as control and received oral administration of phosphate buffer saline; group B received 800 mg/kg A. digitata only; group C were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride; group D were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride and treated with 800 mg/kg aqueous extract of A. digitata; group E received 300 mg/kg vitamin E only; group F were injected intraperitoneally with single dose 2 mg/kg b.w cadmium chloride and treated with 300 mg/kg vitamin E. After 21 days, the animals were sacrificed by cervical dislocation, the testes were excised fixed in Bouins fluids for histological analysis and the other homogenized in 5% sucrose solution for determination of tissue malondialdehyde (MDA) and antioxidant enzyme activity, biochemical assay. Results The group treated with cadmium chloride plus A. digitata caused significant decrease in MDA levels with significant increase (p<0.05) in antioxidant activities and biochemical enzymes when compared to cadmium chloride only group. Conclusions Aqueous extract of A. digitata appears to have ameliorative effect against cadmium chloride-induced testicular damage. This could be attributed to the presence of polyphenolic compound.

scholarly journals Aluminium phosphide-induced testicular toxicity through oxidative stress in Wistar rats: Ameliorative role of hesperidin

2018 ◽  
Vol 2 ◽  
pp. 239784731881279 ◽  
Author(s):  
Olusegun Kayode Afolabi ◽  
Adedoja Dorcas Wusu ◽  
Regina Ugbaja ◽  
John Olabode Fatoki
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Sperm Count ◽  
Sperm Parameters ◽  
Lipid Hydroperoxides ◽  
Testicular Toxicity ◽  
Testicular Damage ◽  
Histological Alterations ◽  
Aluminium Phosphide

The present study was designed to investigate aluminium phosphide (ALP)-induced testicular toxicity, including its effects on sperm parameters and histological alterations in Wistar rats, and the possible protective role of hesperidin (HSD). Oral administration of ALP at 1.15 mg/kg body weight (1/10 LD50) for 30 days resulted in a significant increase in testicular malondialdehyde, lipid hydroperoxides, and oxidized protein levels. These indicators of oxidative stress were accompanied by decreased activity of the antioxidant enzymes superoxide dismutase, catalase and glutathione peroxidase, followed by a drastic reduction in the non-enzymatic antioxidant indices of glutathione and total antioxidant capacity when compared to control. Furthermore, ALP treatment produced a marked reduction in sperm count, motility and viability while increasing abnormal sperm morphology and adverse histopathological changes in testis. Co-administration with HSD significantly ameliorated ALP-induced testicular damage by suppressing oxidative stress indices and enhancing antioxidant status while also improving the sperm parameters and histological alterations in ALP-treated rats. The results of the present study indicated that testicular toxic effects of ALP are due to oxidative imbalance and that HSD could be a potential therapeutic agent against ALP-induced testicular damage.

scholarly journals Role of vitamin E in glutathione-induced oxidant stress: methemoglobin, lipid peroxidation, and hemolysis

1977 ◽  
Vol 18 (5) ◽  
pp. 635-644
Author(s):  
N R Brownlee ◽  
J J Huttner ◽  
R V Panganamala ◽  
D G Cornwell
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Oxidant Stress

Role of lipid peroxidation in the testicular damage in experimentally varicocele induced rats

1998 ◽  
Vol 26 (3) ◽  
pp. S220-S220 ◽  
Author(s):  
Zafer Aybek ◽  
Mehmet H. Koseoglu ◽  
Diler Aslan ◽  
Ender Duzcan ◽  
Nurten Saydam ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Testicular Damage

scholarly journals Role of Vitamin E on Indomethacin induced low pH of Seminal Fluid in Long Evans Rats

1970 ◽  
Vol 8 (1) ◽  
pp. 13-15
Author(s):  
Md Jahangir Alam ◽  
Humaira Naushaba ◽  
Uttam Kumar Paul ◽  
Tahmina Begum ◽  
Sunjida Shahriah ◽  
...  
Keyword(s):  
Body Weight ◽  
Vitamin E ◽  
Seminal Fluid ◽  
Low Ph ◽  
The Other ◽  
Testicular Damage ◽  
Other Hand ◽  
Long Evans ◽  
Different Doses

Context: Indomethacin is the most commonly and widely used nonsteroidal antinflammatory analgesic and antipyretic drug. Though it is effective drug in various diseases, indomethacin causes inhibition of spermatogenesis by lowering the pH of seminal fluid leading to infertility. On the other hand, vitamin E enhances spermatogenesis by increasing pH of the seminal fluid. Therefore, the present study was designed to observe the protective role of vitamin E on indomethacin induced low pH of seminal fluid in testicular damage. Objective: To observe the effects of vitamin E on indomethacin induced low pH of seminal fluid in testicular damage in Long Evans rats. Study design: An experimental study. Place and period of study: The study was carried out in the Department of Anatomy, Sir Salimullah Medical College, Dhaka in the period of August, 2005 to June, 2006. Materials and methods: Eightyfour mature Long Evans male rats were divided into four groups (I, II, III and IV). The rats of group I, II and III were treated with indomethacin at different doses and duration. Group IV rats were treated with indomethacin plus vitamin E at different doses for 49 days. The pH of seminal fluid were measured biochemically. Results: There was significant reduction (P<0.001) of pH of seminal fluid when the rats were treated with indomethacin at low (2 mg/kg body weight/day) and high (10 mg/kg body weight/day) doses for 7, 14 and 42 days, respectively. On the other hand, rats treated with indomethacin plus vitamin E for 49 days showed increase in pH of seminal fluid compared to other groups (P<0.001). Conclusion: It can be concluded from the study that vitamin E has potential role in the prevention of the antispermatogenic effects of indomethacin by increasing the pH of seminal fluid. Key words: seminal fluid; indomethacin; vitamin E   DOI: 10.3329/bja.v8i1.6103 Bangladesh Journal of Anatomy January 2010, Vol. 8 No. 1 pp. 13-15

scholarly journals Sex Differences in Postischemic Neuronal Necrosis in Gerbils

1991 ◽  
Vol 11 (2) ◽  
pp. 292-298 ◽  
Author(s):  
Edward D. Hall ◽  
Kay E. Pazara ◽  
Kelley L. Linseman
Keyword(s):  
Lipid Peroxidation ◽  
Cerebral Cortex ◽  
Vitamin E ◽  
Protective Efficacy ◽  
Neuronal Population ◽  
Mongolian Gerbils ◽  
Neuronal Necrosis ◽  
Male And Female ◽  
Endogenous Estrogen

Twenty-four hour postischemic neuronal necrosis was compared in male vs. female Mongolian gerbils subjected to a 3-h period of severe incomplete hemispheric ischemia produced by unilateral carotid occlusion. The incidence of stroke-prone males was 42.9% versus 26.7% for the females. Among the stroke-prone animals, the males displayed significantly greater neuronal necrosis at 24 h after ischemia compared to the females in the cerebral cortex and CA, region of the hippocampus. In the CA, region of the stroke-prone males, only 2.0% of the normal neuronal population remained by 24 h compared to 36.8% in the stroke-prone females (p < 0.02). In the cerebral cortex, the males had only 19.9% of normal versus 58.2% in the females (p < 0.05). In a second series of mechanistic experiments, no differences in cortical blood flow (CBF) were disclosed between preselected male and female stroke-prone animals before, during, or for 2 h after ischemia. As with the CBF, the extent of cortical extracellular hypocalcia during ischemia did not differ significantly. However, the degree of postischemic recovery of cortical extracellular calcium was significantly better in the females from 30 min to 2 h after reperfusion. In the same experiments, hemispheric vitamin E levels were measured at the 2 h time point as an index of postischemic brain lipid peroxidation. No difference in baseline vitamin E levels was observed between male and female sham-operated gerbils. In the males subjected to 3 h of ischemia plus 2 h of reperfusion, the hemispheric vitamin E decreased by 43.5% compared to the sham-operated males. In contrast, the females displayed only a 4.2% decline (p < 0.05 versus males). Previous studies showing the protective efficacy of antioxidants in this model have suggested an important role of oxygen radical-induced lipid peroxidation. Thus, it is proposed that the lesser ischemic vulnerability of females may be based upon an antioxidant effect of endogenous estrogen. Indeed, estrogen was found to be a more potent inhibitor of iron-catalyzed lipid peroxidation in brain tissue than vitamin E.

Mécanisme moléculaire de l'effet protecteur de la vitamine E dans l'athérosclérose

2002 ◽  
Vol 80 (7) ◽  
pp. 662-669 ◽  
Author(s):  
Abdelouahed Khalil
Keyword(s):  
Lipid Peroxidation ◽  
Vitamin E ◽  
Vascular Diseases ◽  
Epidemiologic Studies ◽  
Low Density Lipoproteins ◽  
Alpha Tocopherol ◽  
Low Density ◽  
Prooxidant Effect

Oxidation of low-density lipoproteins constitutes the first step of a very complex process leading to atherosclerosis. Vitamin E, and principally alpha-tocopherol, is considered as the principal inhibitor of lipid peroxidation. Some studies showed the beneficial role of vitamin E in the prevention and reduction of atherosclerosis and its associated pathologies. However, other in vitro studies advance a prooxidant role of vitamin E. The results of the epidemiologic studies are difficult to generalize without taking account of the clinical randomized tests. In this work, we reviewed the principal studies devoted to the role of vitamin E and discussed the assumption of a prooxidant effect of this molecule.Key words: vitamin E, low-density lipoproteins (LDL), lipid peroxidation, cardio-vascular diseases.

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