scholarly journals Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Carlos Carpena-Torres ◽  
Jesús Pintor ◽  
María Jesús Pérez de Lara ◽  
Fernando Huete-Toral ◽  
Almudena Crooke ◽  
...  
Keyword(s):  
Dry Eye ◽  
Benzalkonium Chloride ◽  
Goblet Cells ◽  
Original Model ◽  
Control Group ◽  
Conjunctival Hyperemia ◽  
Tear Secretion ◽  
Eye Model ◽  
Breakup Time ◽  
Corneal Staining

Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).

scholarly journals Efficacy of Artificial Tears Based on an Extract of Artemia salina Containing Dinucleotides in a Rabbit Dry Eye Model

2021 ◽  
Vol 22 (21) ◽  
pp. 11999
Author(s):  
Carlos Carpena-Torres ◽  
Jesus Pintor ◽  
Fernando Huete-Toral ◽  
Alba Martin-Gil ◽  
Candela Rodríguez-Pomar ◽  
...  
Keyword(s):  
Dry Eye ◽  
Randomized Study ◽  
Goblet Cells ◽  
Artemia Salina ◽  
Mrna Levels ◽  
Artificial Tears ◽  
Conjunctival Hyperemia ◽  
Eye Model ◽  
Beneficial Effects ◽  
High Concentrations

(1) Background: Artemia salina is a brine shrimp containing high concentrations of dinucleotides, molecules with properties for dry eye treatment. For this reason, the purpose of the study was to evaluate the effect of the artificial tears based on an extract of Artemia salina in a rabbit dry eye model. (2) Methods: A prospective and randomized study was carried out. Twenty rabbits were divided into 4 groups (n = 5, each group): healthy rabbits, dry eye rabbits, dry eye rabbits treated with hypromellose (HPMC), and dry eye rabbits treated with Artemia salina. Dry eye was induced by the topical instillation of 0.2% benzalkonium chloride. The measurements were performed before and after the treatment for 5 consecutive days. (3) Results: The topical instillation of artificial tears containing Artemia salina showed beneficial effects on tear secretion, tear break-up time, corneal staining, the density of Goblet cells, heigh of mucin cloud secreted by these cells, and mRNA levels of IL-1β and MMP9 in conjunctival cells. Compared with the HPMC, there was a statistically significant improvement (p < 0.05) with the Artemia salina in all the variables under study, except for the conjunctival hyperemia, density of Goblet cells, and mRNA levels of IL-6. (4) Conclusions: The potential of artificial tears based on Artemia salina as a secretagogue agent for dry eye treatment was confirmed, opening the door for future clinical trials and studies to extrapolate the findings for dry eye patients.

scholarly journals Evaluation of Dry Eye after Implantable Collamer Lens Surgery

2020 ◽  
Author(s):  
Haiting Chen ◽  
Xueyan Feng ◽  
Guangzeng Niu ◽  
Yuxiang Fan
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Case Series ◽  
Meibomian Gland ◽  
Quality Score ◽  
Control Group ◽  
Ocular Surface Disease Index ◽  
Implantable Collamer Lens ◽  
Lens Surgery ◽  
Breakup Time

<b><i>Objectives:</i></b> To evaluate dry eye (DE) and associated meibomian gland dysfunction parameters after Implantable Collamer Lens (ICL) surgery. <b><i>Methods:</i></b> This is a prospective observational case series. Patients who underwent ICL implantation without previous ocular diseases or ophthalmic treatments were enrolled. Their Ocular Surface Disease Index (OSDI), noninvasive breakup time (NIBUT), meibography, slit-lamp examination of the lid margin, corneal fluorescein staining (CFS), and Schirmer test I were examined preoperatively and at 1 and 3 months postoperatively. <b><i>Results:</i></b> A total of 117 eyes of 60 patients were enrolled, and 107 eyes completed 3-month follow-up period. OSDI, lid marginal abnormality, and meibomian gland (MG) secretion, and meibum quality score were significantly higher at 1 month postoperatively and recovered partially at 3 months after surgeries, while NIBUT was significantly decreased all the time. Patients with previous DE symptoms (OSDI score ≥12) showed not only lower Schirmer and TBUT values but also higher CFS, lid margin score, MG loss, MG secretion, and meibum quality scores compared with those in the control group after operations. Low Schirmer, NIBUT values, and high meibum quality score were determined as risk factors for DE symptoms after ICL surgery. <b><i>Conclusions:</i></b> ICL implantation has a bad influence on the ocular surface and MG functions. The influence may be more obvious in patients with existing DE.

scholarly journals The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Treated with Antiglaucoma Eye Medications

Diagnostics ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 20 ◽  
Author(s):  
Yukari Yagi-Yaguchi ◽  
Takashi Kojima ◽  
Kazunari Higa ◽  
Murat Dogru ◽  
Osama MA. Ibrahim ◽  
...  
Keyword(s):  
Ocular Surface ◽  
Vital Staining ◽  
Goblet Cells ◽  
Surface Epithelium ◽  
Control Group ◽  
Eye Drops ◽  
Group 4 ◽  
Group 2 ◽  
Corneal Staining ◽  
Group 1

Anti-glaucoma eye drop treatment often induces dry eyes and can lead to poor medication adherence. This study aimed to investigate the effects of 3% diquafosol sodium eye drops on tear function and the ocular surface epithelium in Sod1−/− mice after treatment with anti-glaucoma eye drops. The mice were divided into four groups: group 1, control group; group 2, anti-glaucoma eye drop; group 3, anti-glaucoma eye drops followed by a secretagogue eye drop (3% diquafosol); and group 4, simultaneous anti-glaucoma and secretagogue eye drop. Mice underwent assessments of tear quantity, tear film breakup time, and vital staining score. Mice in groups 3 and 4 showed significantly better tear stability and lower corneal staining scores than mice in group 2 after eye drop instillations (p < 0.05). Mice in group 4 showed significantly better tear stability, lower corneal staining scores, and higher goblet cell densities than those in group 1 after eye drop instillations (p < 0.05). The conjunctival epithelium showed stratification and abundance of Muc5AC-positive goblet cells in group 4, whereas thinning with desquamation was observed with a few goblet cells in group 2. Thus, simultaneous administration of 3% diquafosol sodium eye drops with topical anti-glaucoma drops showed favorable effects on tear stability and the corneal epithelium against the ocular surface toxicity inflicted by the anti-glaucoma eye drops.

Delphinidin 3,5-O-diglucoside, a constituent of the maqui berry (Aristotelia chilensis) anthocyanin, restores tear secretion in a rat dry eye model

2014 ◽  
Vol 10 ◽  
pp. 346-354 ◽  
Author(s):  
Shigeru Nakamura ◽  
Junji Tanaka ◽  
Toshihiro Imada ◽  
Hiroshi Shimoda ◽  
Kazuo Tsubota
Keyword(s):  
Dry Eye ◽  
Tear Secretion ◽  
Eye Model ◽  
Aristotelia Chilensis ◽  
Maqui Berry

scholarly journals Effect of IRT5 probiotics on dry eye in the experimental dry eye mouse model

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243176
Author(s):  
Jayoon Moon ◽  
Jin Suk Ryu ◽  
Jun Yeop Kim ◽  
Sin-Hyeog Im ◽  
Mee Kum Kim
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Intestinal Microbiome ◽  
Rt Pcr ◽  
Oral Gavage ◽  
Once Daily ◽  
Tear Secretion ◽  
Eye Model ◽  
Goblet Cell Density

Objective To investigate the clinical effects of IRT5 probiotics in the environmental dry eye model. Methods Eight week old male C57BL/6 mice were randomly divided into two groups; control group (n = 16) received oral gavage of 300 μL phosphate-buffered saline (PBS) alone once daily, IRT5 group (n = 9) received oral gavage of 1 x 109 CFU IRT5 probiotics powder in 300 μL PBS once daily, both groups for 11 to 12 days. Simultaneously, all mice underwent dry eye induction. Tear secretion, corneal staining and conjunctival goblet cell density were evaluated. Quantative real-time polymerase chain reaction (RT-PCR) for inflammation-related markers was performed. 16S ribosomal RNA of fecal microbiome was analyzed and compositional difference, alpha and beta diversities were assessed. Results There was no difference in NEI score but significant increase in tear secretion was observed in IRT5 group (p < 0.001). There was no significant difference in goblet cell density between groups. Quantative RT-PCR of cornea and conjunctiva revealed increased TNF-α expression in IRT5 group (p < 0.001) whereas other markers did not significantly differ from control. IRT5 group had significantly increased species diversity by Shannon index (p = 0.041). Beta diversity of genus by UniFrac principle coordinates analysis showed significant distance between groups (p = 0.001). Compositional differences between groups were observed and some were significantly associated with tear secretion. Multivariate linear regression analysis revealed Christensenellaceae (p = 0.009), Lactobacillus Helveticus group (p = 0.002) and PAC001797_s (p = 0.011) to strongly influence tear secretion. Conclusion In experimental dry eye model, IRT5 probiotics treatment partially improves experimental dry eye by increasing tear secretion which was associated with and influenced by the change in intestinal microbiome. Also, intestinal microbiome may affect the lacrimal gland through a different mechanism other than regulating inflammation.

Comparative analysis of incidence of dry eye syndrome after ReLEx SMILE and FemtoLASIK keratorefractive surgeries

2021 ◽  
pp. 192-196
Author(s):  
A.D. Pilipenko ◽  
◽  
R.A. Burya ◽  
A.V. Romanova ◽  
E.L. Sorokin ◽  
...  
Keyword(s):  
Dry Eye ◽  
Ocular Surface ◽  
Refractive Surgery ◽  
Photorefractive Effect ◽  
Study Groups ◽  
Dry Eye Syndrome ◽  
Control Group ◽  
Significant Difference ◽  
Negative Effect ◽  
Breakup Time

The main factor in pathogenesis of postoperative dry eye syndrome (DES) is damage to subbasal nerve plexus and stromal nerve endings. Taking into account the different principles of photorefractive effect on cornea of FemtoLASIK and ReLEx SMILE technologies, we decided on our own clinical experience to evaluate incidence of this undesirable complication. Purpose. Comparative study of frequency and timing of relief of postoperative DES after FemtoLASIK and ReLEx SMILE in patients with myopia. Material and methods. The study involved 32 patients (64 eyes) with varying degrees of myopia. The patients were divided into 2 groups: 1st group - 17 patients (34 eyes) after FemtoLASIK surgery, 2nd group - 15 people (30 eyes) after ReLEx SMILE procedure. Control group consist of 15 patients with comparable myopia who had not undergone refractive surgery was also observed. Patients underwent an assessment of the OSDI index, Norn and Schirmer tests, as well as degree of staining of anterior ocular surface 1, 6 and 12 months after refractive surgery. Results. There was statistically significant difference in total tear production, in tear breakup time (TBUT) and in the OSDI index between study groups 1 month after surgery in favor of ReLEx SMILE procedure. After 6 months, TBUT after FemtoLASIK significantly differed from control values. 12 months after surgery, studied parameters did not differ significantly from control. Conclusion. According to our datas, ReLEx SMILE procedure has lower negative effect on ocular surface compared to FemtoLASIK technique, which is manifested by less pronounced subjective symptoms of DES, as well as their faster relief. Key words: dry eye syndrome, ReLEx SMILE, FemtoLASIK.

Peculiarities of the syndrome of ‘‘dry eye’’ in children with congenital glaucoma

2013 ◽  
Vol 4 (2) ◽  
pp. 45-51
Author(s):  
Olga Anatolievna Vorontsova ◽  
Vladimir Vsevolodovich Brzheskiy
Keyword(s):  
Conservative Treatment ◽  
Dry Eye ◽  
Congenital Glaucoma ◽  
Control Group ◽  
Healthy Children ◽  
Duration Of Treatment ◽  
Tear Secretion ◽  
Break Up

Authors analyze the results of examination of 33 children with congenital glaucoma in the age from 3 till 17 years old. Two groups were sorted out. Children of the first group were treated with 0.5 % timolol maleat, the minimal duration of treatment was 6 months (6 months–7 years). The second group included patients with congenital glaucoma without conservative treatment. The control group included 226 healthy children in the age from 3 till 17 years old without complaints and symptoms of dry eye. The decrease of tear break-up time was revealed in all children with congenital glaucoma. In patients receiving β-blocking treatment the decrease of basal tear secretion was also detected.

IMPRESSIONABLE CYTOLOGY METHOD IN DIAGNOSTICS OF DRY EYE SYNDROME

2019 ◽  
Vol 64 (6) ◽  
pp. 348-350
Author(s):  
N. M. Zakharova ◽  
I. P. Shabalova ◽  
G. H. Ali-Zade ◽  
A. A. Rjabtseva ◽  
O. N. Vetchinnikova ◽  
...  
Keyword(s):  
Dry Eye ◽  
Squamous Epithelium ◽  
Goblet Cells ◽  
Dry Eye Syndrome ◽  
Eye Disease ◽  
Control Group ◽  
Impression Cytology ◽  
Cytological Examination ◽  
Main Component ◽  
Cytological Sample

Analysis of the cellular composition of the eyeball conjunctival prints and evaluation of the informative of the cytomorphological method of research in the diagnosis of dry eye syndrome (DES). Cytological examination using impression cytology was performed in 72 patients: 60 patients with DES and 12 without eye disease (control group). The main component of cytological sample in patients with DES are (a) squamous epithelium (hyperkeratocytes); (b) groups and clusters of flattened epithelial cells; (c) a small amount (up to five in the preparation) of goblet cells. Impression cytology method improves the accuracy of diagnosis of DES.

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