Delphinidin 3,5-O-diglucoside, a constituent of the maqui berry (Aristotelia chilensis) anthocyanin, restores tear secretion in a rat dry eye model

2014 ◽  
Vol 10 ◽  
pp. 346-354 ◽  
Author(s):  
Shigeru Nakamura ◽  
Junji Tanaka ◽  
Toshihiro Imada ◽  
Hiroshi Shimoda ◽  
Kazuo Tsubota
Keyword(s):  
Dry Eye ◽  
Tear Secretion ◽  
Eye Model ◽  
Aristotelia Chilensis ◽  
Maqui Berry

scholarly journals Effect of IRT5 probiotics on dry eye in the experimental dry eye mouse model

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243176
Author(s):  
Jayoon Moon ◽  
Jin Suk Ryu ◽  
Jun Yeop Kim ◽  
Sin-Hyeog Im ◽  
Mee Kum Kim
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Intestinal Microbiome ◽  
Rt Pcr ◽  
Oral Gavage ◽  
Once Daily ◽  
Tear Secretion ◽  
Eye Model ◽  
Goblet Cell Density

Objective To investigate the clinical effects of IRT5 probiotics in the environmental dry eye model. Methods Eight week old male C57BL/6 mice were randomly divided into two groups; control group (n = 16) received oral gavage of 300 μL phosphate-buffered saline (PBS) alone once daily, IRT5 group (n = 9) received oral gavage of 1 x 109 CFU IRT5 probiotics powder in 300 μL PBS once daily, both groups for 11 to 12 days. Simultaneously, all mice underwent dry eye induction. Tear secretion, corneal staining and conjunctival goblet cell density were evaluated. Quantative real-time polymerase chain reaction (RT-PCR) for inflammation-related markers was performed. 16S ribosomal RNA of fecal microbiome was analyzed and compositional difference, alpha and beta diversities were assessed. Results There was no difference in NEI score but significant increase in tear secretion was observed in IRT5 group (p < 0.001). There was no significant difference in goblet cell density between groups. Quantative RT-PCR of cornea and conjunctiva revealed increased TNF-α expression in IRT5 group (p < 0.001) whereas other markers did not significantly differ from control. IRT5 group had significantly increased species diversity by Shannon index (p = 0.041). Beta diversity of genus by UniFrac principle coordinates analysis showed significant distance between groups (p = 0.001). Compositional differences between groups were observed and some were significantly associated with tear secretion. Multivariate linear regression analysis revealed Christensenellaceae (p = 0.009), Lactobacillus Helveticus group (p = 0.002) and PAC001797_s (p = 0.011) to strongly influence tear secretion. Conclusion In experimental dry eye model, IRT5 probiotics treatment partially improves experimental dry eye by increasing tear secretion which was associated with and influenced by the change in intestinal microbiome. Also, intestinal microbiome may affect the lacrimal gland through a different mechanism other than regulating inflammation.

scholarly journals Restoration of Tear Secretion in a Murine Dry Eye Model by Oral Administration of Palmitoleic Acid

Nutrients ◽  
2017 ◽  
Vol 9 (4) ◽  
pp. 364 ◽  
Author(s):  
Shigeru Nakamura ◽  
Yuki Kimura ◽  
Daisuke Mori ◽  
Toshihiro Imada ◽  
Yusuke Izuta ◽  
...  
Keyword(s):  
Oral Administration ◽  
Dry Eye ◽  
Palmitoleic Acid ◽  
Tear Secretion ◽  
Eye Model

scholarly journals Oral Administration of Royal Jelly Restores Tear Secretion Capacity in Rat Blink-Suppressed Dry Eye Model by Modulating Lacrimal Gland Function

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e106338 ◽  
Author(s):  
Toshihiro Imada ◽  
Shigeru Nakamura ◽  
Naoki Kitamura ◽  
Izumi Shibuya ◽  
Kazuo Tsubota
Keyword(s):  
Oral Administration ◽  
Dry Eye ◽  
Lacrimal Gland ◽  
Royal Jelly ◽  
Tear Secretion ◽  
Eye Model ◽  
Gland Function

scholarly journals Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Carlos Carpena-Torres ◽  
Jesús Pintor ◽  
María Jesús Pérez de Lara ◽  
Fernando Huete-Toral ◽  
Almudena Crooke ◽  
...  
Keyword(s):  
Dry Eye ◽  
Benzalkonium Chloride ◽  
Goblet Cells ◽  
Original Model ◽  
Control Group ◽  
Conjunctival Hyperemia ◽  
Tear Secretion ◽  
Eye Model ◽  
Breakup Time ◽  
Corneal Staining

Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).

scholarly journals Effect of persimmon leaves (Diospyros kaki) on goblet cell density and inflammation in experimental dry eye model

2020 ◽  
Vol 63 (1) ◽  
Author(s):  
Kyung-A Kim ◽  
Chung Hyun Lee ◽  
Tae Kyeom Kang ◽  
Sung Jae Yang ◽  
Chang Yong Lee ◽  
...  
Keyword(s):  
Cell Density ◽  
Dry Eye ◽  
Goblet Cell ◽  
Diospyros Kaki ◽  
Eye Model ◽  
Goblet Cell Density

scholarly journals Refractory Dry Eye Syndrome after Transconjunctival Excision of the Palpebral Lobe of the Lacrimal Gland

Medicina ◽  
2021 ◽  
Vol 57 (6) ◽  
pp. 608
Author(s):  
Yong-Jae Lee ◽  
Han-Sol Choi ◽  
Seong-Jun Park ◽  
Hae-Jung Sun ◽  
Sun-Young Jang
Keyword(s):  
Female Patient ◽  
Dry Eye ◽  
Lacrimal Gland ◽  
Medical Center ◽  
Chief Complaint ◽  
Incisional Biopsy ◽  
Lacrimal Glands ◽  
Palpable Mass ◽  
Tear Secretion ◽  
Palpebral Lobe

The aim of the present study was to report two cases of refractory dry eye syndrome (DES) after transconjunctival excision of the palpebral lobe of the lacrimal gland. A 25-year-old female patient with a chief complaint of a palpable mass in both upper eyelids visited our medical center. Preoperative orbital computer tomography showed high-attenuation lesions in both lacrimal glands. Incisional biopsy of the lacrimal gland palpebral lobe via transconjunctival incision was performed in January 2019. At 1 month after the biopsy, a lack of tears and persistent corneal erosions were found in both eyes. Artificial tears, punctal occlusion, autologous serum eye drops, and therapeutic contact lenses were applied in an attempt to control the dry eye symptoms. The patient continues to suffer from intractable DES at 2.5 years after the procedure. The second case involved a 52-year-old female patient who visited our medical center with a chief complaint of a palpable mass in both upper eyelids. Bilateral orbital tumors were diagnosed with preoperative magnetic resonance imaging. An incisional biopsy of the lacrimal gland was performed. Immunoglobulin G4-related dacryoadenitis was confirmed through lacrimal palpebral lobe incisional biopsy. Intractable DES and corneal erosion of her left eye persisted thereafter. A transconjunctival incision is an effective approach for minimizing postoperative scars and is suitable for the biopsy of tumors that are visible through the conjunctiva. After a biopsy of the palpebral lobe of the main lacrimal glands, the secretion of reflex tears decreases due to damage to the secreting ducts of the main lacrimal glands. However, total tear secretion can be maintained by basal tear secretion from the accessory lacrimal glands. In this report, we describe two cases of refractory DES due to decreased total tear secretion, although only the palpebral lobes of the main lacrimal glands were biopsied.

A novel beverage rich in antioxidant phenolics: Maqui berry (Aristotelia chilensis) and lemon juice

LWT ◽  
2012 ◽  
Vol 47 (2) ◽  
pp. 279-286 ◽  
Author(s):  
Amadeo Gironés-Vilaplana ◽  
Pedro Mena ◽  
Cristina García-Viguera ◽  
Diego A. Moreno
Keyword(s):  
Lemon Juice ◽  
Aristotelia Chilensis ◽  
Maqui Berry

Tear secretion from the lacrimal gland: variations in normal versus dry eyes

2021 ◽  
pp. bjophthalmol-2020-318159
Author(s):  
Swati Singh ◽  
Swapna S Shanbhag ◽  
Sayan Basu
Keyword(s):  
Dry Eye ◽  
Flow Rate ◽  
Lacrimal Gland ◽  
Healthy Subjects ◽  
Time Lag ◽  
Similar Time ◽  
Dry Eyes ◽  
Tear Secretion ◽  
Direct Assessment ◽  
Tear Flow

PurposeTo investigate the secretory status of the main lacrimal gland in healthy and dry eye disease (DED) via fluorescein-assisted direct assessment of tear secretion from the palpebral lobes.MethodsIncluded were 25 healthy subjects (50 lobes) and 75 subjects with DED (cicatrising conjunctivitis (CC, n=27), evaporative dry eyes (EDE, n=25) and Sjogren’s syndrome (SS, n=23)). Analysed parameters included number and location of ductular openings, tear flow rate per gland and per ductule, and the time lag for the initiation of secretion.ResultsDuctular openings could be observed in all patients with EDE and healthy subjects whereas only 33% (18/54) glands of CC patients and 67% glands (31/46) patients with SS revealed ductules. The median number of ductules per lobe was 4 in normal (range 3–5), 3 in EDE (3–6), 1 in SS (0–3) and 0 in CC group (0–3) (p<0.000001). The median tear flow rate per lobe in CC (0.00 μL/min) and SS (0.21 μL/min) was significantly lesser than normal lobes (1.05 μL/min, and EDE (0.99 μL/min eyes. The tear flow rate differed significantly between SS and CC group (p<0.0001). The maximum time lag occurred in the CC group (median, 20 s), followed by the SS group (median, 1.5 s) whereas the EDE group had similar time lag (<1 s) as of normal glands (p<0.0001).ConclusionDirect assessment of tear secretion from the palpebral lobe demonstrates significant differences between EDE, aqueous deficient dry eye and dry eye in CC.

scholarly journals Maqui berry vs Sloe berry – Liquor-based Beverage for New Development

2015 ◽  
Vol 10 (1) ◽  
pp. 1934578X1501000
Author(s):  
Amadeo Gironés-Vilaplana ◽  
Diego A. Moreno ◽  
Cristina García-Viguera
Keyword(s):  
The Other ◽  
Northern Spain ◽  
Organoleptic Properties ◽  
Beneficial Effects ◽  
Other Hand ◽  
New Development ◽  
Aristotelia Chilensis ◽  
Maqui Berry ◽  
Over Time

“Pacharán” is an aniseed liquor-based beverage made with sloe berry ( Prunus spinosa L.) that has been produced in northern Spain. On the other hand, maqui berry ( Aristotelia chilensis) is a common edible berry from Chile, and currently under study because of its multiple beneficial effects on health. The aim of this work was to design a new aniseed liquor-based beverage with maqui berry, as an industrial alternative to a traditional alcoholic product with bioactive berries. The characterization of its composition, compared with the traditional “Pacharán”, and its evolution during maceration (6 and 12 months) showed that the new maqui liquor had significantly-higher anthocyanin retention over time. More studies on the organoleptic properties and bioactivity are underway.

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