scholarly journals Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Shilian Wu ◽  
Yanqiong Zhang ◽  
Long Yang ◽  
Can-Peng Li
Keyword(s):  
Epigallocatechin Gallate ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sunset Yellow ◽  
Sensing Platform ◽  
Potential Applications ◽  
Displacement Assay ◽  
Signal Probe ◽  
Target Molecules

This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.

scholarly journals A Fluorescence Sensing Determination of 2, 4, 6-Trinitrophenol Based on Cationic Water-Soluble Pillar[6]arene Graphene Nanocomposite

Sensors ◽  
2018 ◽  
Vol 19 (1) ◽  
pp. 91 ◽  
Author(s):  
Xiaoping Tan ◽  
Tingying Zhang ◽  
Wenjie Zeng ◽  
Shuhua He ◽  
Xi Liu ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Fluorescence Probe ◽  
Water Soluble ◽  
Fluorescence Sensing ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
Potential Applications ◽  
Water And Soil Samples ◽  
Graphene Nanocomposite

We describe a selective and sensitive fluorescence platform for the detection of trinitrophenol (TNP) based on competitive host–guest recognition between pyridine-functionalized pillar[6]arene (PCP6) and a probe (acridine orange, AO) that used PCP6-functionalized reduced graphene (PCP6-rGO) as the receptor. TNP is an electron-deficient and negative molecule, which is captured by PCP6 via electrostatic interactions and π–π interactions. Therefore, a selective and sensitive fluorescence probe for TNP detection is developed. It has a low detection limit of 0.0035 μM (S/N = 3) and a wider linear response of 0.01–5.0 and 5.0–125.0 for TNP. The sensing platform is also used to test TNP in two water and soil samples with satisfying results. This suggests that this approach has potential applications for the determination of TNP.

scholarly journals A Facile and High Selectivity Novel Fluorescence Sensing Determination of 2, 4, 6-Trinitrophenol Based on Cationic Water-Soluble Pillar[6]arene Graphene Nanocomposite

2018 ◽  
Author(s):  
Xiaoping Tan ◽  
Tingying Zhang ◽  
Wenjie Zeng ◽  
Shuhua He ◽  
Xi Liu ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Water Soluble ◽  
Fluorescence Sensing ◽  
Sensing Platform ◽  
Reduced Graphene ◽  
Potential Applications ◽  
Water And Soil Samples ◽  
Graphene Nanocomposite ◽  
Pi Interactions

We describe a selective and sensitive fluorescence platform for the detection of trinitrophenol (TNP) based on competitive host–guest recognition between pyridine-functionalized pillar[6]arene (PCP6) and probe (acridine orange, AO) that used PCP6-functionalized reduced graphene (PCP6-rGO) as the receptor. TNP is an electron-deficient and negative molecule which is captured by PCP6 via electrostatic interactions and π-π interactions. Therefore, a selective and sensitive fluorescence sensor for TNP detection is developed. It has a low detection limit of 0.0035 μM (S/N=3) and a wider linear response of 0.01−5.0 and 5.0−125.0 for TNP. The sensing platform is also used to test TNP in two water and soil samples with satisfying results. This suggests that this approach has potential applications for the determination of TNP.

Fluorescence chemosensors for hydrogen sulfide detection in biological systems

The Analyst ◽  
2015 ◽  
Vol 140 (6) ◽  
pp. 1772-1786 ◽  
Author(s):  
Zhi Guo ◽  
Guiqiu Chen ◽  
Guangming Zeng ◽  
Zhongwu Li ◽  
Anwei Chen ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Biological Systems ◽  
Sulfate Reducing Bacteria ◽  
Fluorescence Sensing ◽  
Sulfate Reducing ◽  
Potential Applications ◽  
Reducing Bacteria

The development of H2S fluorescence-sensing strategies and their potential applications in the determination of sulfate-reducing bacteria activity.

Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA

The Analyst ◽  
2016 ◽  
Vol 141 (15) ◽  
pp. 4719-4724 ◽  
Author(s):  
Yaqing Chang ◽  
Zhe Zhang ◽  
Huiqing Liu ◽  
Nan Wang ◽  
Jilin Tang
Keyword(s):  
Fluorescence Quenching ◽  
Label Free ◽  
Quenching Mechanism ◽  
Fluorescence Sensing ◽  
Single Stranded Dna ◽  
Cobalt Oxyhydroxide ◽  
Sensing Platform ◽  
Label Free Detection ◽  
Fluorescence Quenching Mechanism ◽  
Coooh Nanoflakes

In this study, we investigated the interaction of cobalt oxyhydroxide (CoOOH) nanoflakes with DNA and their fluorescence quenching mechanism of a FAM-labeled single-stranded DNA (ssDNA) probe.

scholarly journals Switching of electrochemical characteristics of redox protein upon specific biomolecular interactions

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6118-6121 ◽  
Author(s):  
Man Yi Ho ◽  
Sarah A. Goodchild ◽  
Pedro Estrela ◽  
Daping Chu ◽  
Piero Migliorato
Keyword(s):  
Specific Interaction ◽  
Biomolecular Interactions ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Protein Sensing ◽  
Redox Protein ◽  
Free Protein ◽  
Sensing Platform ◽  
Target Molecules

Label-free protein sensing platform based on a simplified and standardized immobilization process with Azurin redox self-assembled monolayer is fabricated. A significant change in the electrochemical characteristics of the assay upon specific interaction with target molecules is observed.

scholarly journals A Label-free Optical Sensing Platform for Beta-glucosidase Activity Using Protein-inorganic Hybrid Nanoflowers

2021 ◽  
Author(s):  
Ziping Liu ◽  
Shasha Liu ◽  
Decai Gao ◽  
Yanan Li ◽  
Ye Tian ◽  
...  
Keyword(s):  
Optical Sensing ◽  
Enzymatic Reaction ◽  
Label Free ◽  
Inorganic Hybrid ◽  
Sensing System ◽  
Amplex Red ◽  
Sensing Platform ◽  
Synthesis Strategy ◽  
Beta Glucosidase

Abstract In this work, a convenient, label-free and dual-signal readout optical sensing platform for the sensitively and selectively determination of beta-glucosidase (β-Glu) activity was reported using protein-inorganic hybrid nanoflowers [BSA-Cu3(PO4)2·3H2O] possessing peroxidase-mimicking activity. The nanoflowers (NFs) were facilely synthesized through a self-assembled synthesis strategy at room temperature. The as-prepared NFs could catalytically convert the colorless and non-fluorescent Amplex Red into colored and highly fluorescent resorufin in the presence of hydrogen peroxide via electron transfer process. β-Glu could hydrolyze cyanogenic glycoside, using amygdalin (Amy) as a model, into cyanide ions (CN-), which can subsequently efficiently suppress the catalytic activity of NFs, accompanied with the fluorescence decrease and the color fading. The concentration of CN- was controlled by β-Glu-triggered enzymatic reaction of Amy. Thus, a sensing system was established for fluorescent and visual determination of β-Glu activity. Under the optimum conditions, the present fluorescent and visual bimodal sensing platform exhibited good sensitivity for β-Glu activity assay with a detection limit of 0.33 U·L-1. The sensing platform was further applied to determinate β-Glu in real samples and satisfactory results were attained. Additionally, the label-free optical sensing system can potentially be a promising candidate for β-Glu inhibitors screening.

scholarly journals Novel Competitive Fluorescence Sensing Platform for L-Carnitine Based on Cationic Pillar[5]arene Modified Gold Nanoparticles

2018 ◽  
Author(s):  
Xiaoping Tan ◽  
Yang Yang ◽  
Shasha Luo ◽  
Zhong Zhang ◽  
Wenjie Zeng ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Drugs Of Abuse ◽  
Hydrophobic Interactions ◽  
Molecular Size ◽  
High Sensitivity ◽  
Fluorescence Sensing ◽  
Safety Issues ◽  
Sensing Platform ◽  
Potential Applications ◽  
Sensitivity And Selectivity

A supramolecular host-guest interaction and sensing between cationic pillar[5]arenes (CP5) and L-carnitine were developed by the competitive host-guest recognition for the first time. The fluorescence sensing platform was constructed by CP5 functionalized Au nanoparticles (PP5@Au-NPs) as receptor and probe (rhodamine 123, R123), which shown a high sensitivity and selectivity to L-carnitine detection. Due to the property of the negative charge and molecular size of L-carnitine, it can be highly captured by the CP5 via electrostatic interactions and hydrophobic interactions. The mechanism of host-guest between PP5 and L-carnitine was studied by 1H NMR and molecular docking, which indicated more affinity binding force of PP5 with L-carnitine. Therefore, a selective and sensitive fluorescent method was developed. It has a linear response of 0.1–2.0 and 2.0–25.0 μM and a detection limit of 0.067 μM (S/N = 3) for L-carnitine. The fluorescent sensing platform was also used to detect L-carnitine in human serum and milk samples, which provided potential applications of detection drugs of abuse, and had path for guarding a serious food safety issues.   

scholarly journals A label-free and sensitive photoluminescence sensing platform based on long persistent luminescence nanoparticles for the determination of antibiotics and 2,4,6-trinitrophenol

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 5714-5720 ◽  
Author(s):  
Xi Zhang ◽  
Na-Yan Xu ◽  
Qiong Ruan ◽  
Dan-Qing Lu ◽  
Yun-Hui Yang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Persistent Luminescence ◽  
Label Free ◽  
Low Background ◽  
Background Interference ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Visualized Detection

A highly sensitive luminescence sensor based on PLNPs for visualized detection of antibiotic and organic explosives was developed, which could eliminate the background interference, allowing low background and therefore high sensitivity.

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