scholarly journals Switching of electrochemical characteristics of redox protein upon specific biomolecular interactions

The Analyst ◽  
2014 ◽  
Vol 139 (23) ◽  
pp. 6118-6121 ◽  
Author(s):  
Man Yi Ho ◽  
Sarah A. Goodchild ◽  
Pedro Estrela ◽  
Daping Chu ◽  
Piero Migliorato
Keyword(s):  
Specific Interaction ◽  
Biomolecular Interactions ◽  
Electrochemical Characteristics ◽  
Label Free ◽  
Self Assembled Monolayer ◽  
Protein Sensing ◽  
Redox Protein ◽  
Free Protein ◽  
Sensing Platform ◽  
Target Molecules

Label-free protein sensing platform based on a simplified and standardized immobilization process with Azurin redox self-assembled monolayer is fabricated. A significant change in the electrochemical characteristics of the assay upon specific interaction with target molecules is observed.

scholarly journals Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Shilian Wu ◽  
Yanqiong Zhang ◽  
Long Yang ◽  
Can-Peng Li
Keyword(s):  
Epigallocatechin Gallate ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sunset Yellow ◽  
Sensing Platform ◽  
Potential Applications ◽  
Displacement Assay ◽  
Signal Probe ◽  
Target Molecules

This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.

scholarly journals Label-free protein sensing by employing blue phase liquid crystal

2017 ◽  
Vol 8 (3) ◽  
pp. 1712 ◽  
Author(s):  
Mon-Juan Lee ◽  
Chung-Huan Chang ◽  
Wei Lee
Keyword(s):  
Liquid Crystal ◽  
Label Free ◽  
Protein Sensing ◽  
Free Protein ◽  
Phase Liquid ◽  
Blue Phase

scholarly journals Super-sensitivity in label-free protein sensing using a nanoslot nanolaser

2011 ◽  
Vol 19 (18) ◽  
pp. 17683 ◽  
Author(s):  
Shota Kita ◽  
Shoji Hachuda ◽  
Shota Otsuka ◽  
Tatsuro Endo ◽  
Yasunori Imai ◽  
...  
Keyword(s):  
Label Free ◽  
Protein Sensing ◽  
Free Protein

DNA base-stacking assay utilizing catalytic hairpin assembly-induced gold nanoparticle aggregation for colorimetric protein sensing

2016 ◽  
Vol 52 (22) ◽  
pp. 4167-4170 ◽  
Author(s):  
Chia-Chen Chang ◽  
Chie-Pein Chen ◽  
Chen-Yu Chen ◽  
Chii-Wann Lin
Keyword(s):  
Gold Nanoparticle ◽  
Label Free ◽  
Colorimetric Sensing ◽  
Base Stacking ◽  
Nanoparticle Aggregation ◽  
Protein Sensing ◽  
Sensing Platform ◽  
Dna Base ◽  
Gold Nanoparticle Aggregation ◽  
Catalytic Hairpin Assembly

A label-free and enzyme-free colorimetric sensing platform for the amplified detection of fibronectin was developed based on an ingenious combination of catalytic hairpin assembly and a base stacking hybridization-based gold nanoparticle aggregation strategy.

scholarly journals Common-path interferometric label-free protein sensing with resonant dielectric nanostructures

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Isabel Barth ◽  
Donato Conteduca ◽  
Christopher Reardon ◽  
Steven Johnson ◽  
Thomas F. Krauss
Keyword(s):  
Label Free ◽  
Protein Sensing ◽  
Free Protein ◽  
Common Path

On-chip bulk-index concentration and direct, label-free protein sensing utilizing an optical grated-waveguide cavity

2012 ◽  
Vol 174 ◽  
pp. 602-608 ◽  
Author(s):  
S.V. Pham ◽  
M. Dijkstra ◽  
A.J.F. Hollink ◽  
L.J. Kauppinen ◽  
R.M. de Ridder ◽  
...  
Keyword(s):  
Label Free ◽  
Protein Sensing ◽  
Free Protein ◽  
On Chip ◽  
Direct Label
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