scholarly journals Benzo[g]coumarin-Based Fluorescent Probes for Bioimaging Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuna Jung ◽  
Junyang Jung ◽  
Youngbuhm Huh ◽  
Dokyoung Kim
Keyword(s):  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Molecular Probes ◽  
Important Species ◽  
Disease Biomarkers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Photophysical Parameters ◽  
Absorbing Property ◽  
Linear Shape

Benzo[g]coumarins, which consist of coumarins fused with other aromatic units in the linear shape, have recently emerged as an interesting fluorophore in the bioimaging research. The pi-extended skeleton with the presence of electron-donating and electron-withdrawing substituents from the parent coumarins changes the basic photophysical parameters such as absorption and fluorescence emission significantly. Most of the benzo[g]coumarin analogues show red/far-red fluorescence emission with high two-photon absorbing property that can be applicable for the two-photon microscopy (TPM) imaging. In this review, we summarized the recently developed benzo[g]coumarin analogues including photophysical properties, synthesis, and applications for molecular probes that can sense biologically important species such as metal ions, cell organs, reactive species, and disease biomarkers.

scholarly journals Optical clearing of living brains with MAGICAL to extend in vivo imaging

2019 ◽  
Author(s):  
Kouichirou Iijima ◽  
Takuto Oshima ◽  
Ryosuke Kawakami ◽  
Tomomi Nemoto
Keyword(s):  
Fluorescence Emission ◽  
Cortical Layer ◽  
Fluorescence Emission Spectrum ◽  
Spatiotemporal Resolution ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Brain Functions ◽  
Hippocampal Ca1 ◽  
Layer V

AbstractTo understand brain functions, it is important to observe directly how multiple neural circuits are performing in living brains. However, due to tissue opaqueness, observable depth and spatiotemporal resolution are severely degraded in vivo. Here, we propose an optical brain clearing method for in vivo fluorescence microscopy, termed MAGICAL (Magical Additive Glycerol Improves Clear Alive Luminance). MAGICAL enabled two-photon microscopy to capture vivid images with fast speed, at cortical layer V and hippocampal CA1 in vivo. Moreover, MAGICAL promoted conventional confocal microscopy to visualize finer neuronal structures including synaptic boutons and spines in unprecedented deep regions, without intensive illumination leading to phototoxic effects. Fluorescence Emission Spectrum Transmissive Analysis (FESTA) showed that MAGICAL improved in vivo transmittance of shorter wavelength light, which is vulnerable to optical scattering thus unsuited for in vivo microscopy. These results suggest that MAGICAL would transparentize living brains via scattering reduction.

scholarly journals Lanthanide nano-drums: a new class of molecular nanoparticles for potential biomedical applications

2014 ◽  
Vol 175 ◽  
pp. 241-255 ◽  
Author(s):  
Richard A. Jones ◽  
Annie J. Gnanam ◽  
Jonathan F. Arambula ◽  
Jessica N. Jones ◽  
Jagannath Swaminathan ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Photophysical Properties ◽  
Diagnostic Systems ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Self Assembling ◽  
Molecular Nanoparticles

We are developing a new class of lanthanide-based self-assembling molecular nanoparticles as potential reporter molecules for imaging, and as multi-functional nanoprobes or nanosensors in diagnostic systems. These lanthanide “nano-drums” are homogeneous 4d–4f clusters approximately 25 to 30 Å in diameter that can emit from the visible to near-infrared (NIR) wavelengths. Here, we present syntheses, crystal structures, photophysical properties, and comparative cytotoxicity data for six nano-drums containing either Eu, Tb, Lu, Er, Yb or Ho. Imaging capabilities of these nano-drums are demonstrated using epifluorescence, total internal reflection fluorescence (TIRF), and two-photon microscopy. We discuss how these molecular nanoparticles can to be adapted for a range of assays, particularly by taking advantage of functionalization strategies with chemical moieties to enable conjugation to protein or nucleic acids.

A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells

2018 ◽  
Vol 42 (18) ◽  
pp. 14725-14728 ◽  
Author(s):  
Ling Hu ◽  
Sajid Hussain ◽  
Tianyan Liu ◽  
Yuanzhen Yue ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Molecular Probes ◽  
Living Systems ◽  
Molecular Probe ◽  
Imidazole Derivatives ◽  
Two Photon Microscopy ◽  
Two Photon

Multi-functional florescent dyes capable of acting as molecular probes in living systems under two-photon microscopy, as well as super-resolution nanoscopy, are of great interest.

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Photophysical properties and fluorosolvatochromism of D–π–A thiophene based derivatives

RSC Advances ◽  
2020 ◽  
Vol 10 (71) ◽  
pp. 43459-43471
Author(s):  
Hussain A. Z. Sabek ◽  
Ahmed M. M. Alazaly ◽  
Dina Salah ◽  
Hesham S. Abdel-Samad ◽  
Mohamed A. Ismail ◽  
...  
Keyword(s):  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Time Resolved ◽  
Time Resolved Spectroscopy ◽  
Solvation Models

Solvation-dependent photophysical properties of two push–pull thiophene-based compounds with donor–π–acceptor (D–π–A) structures were investigated using absorption, fluorescence emission and time resolved spectroscopy, and supported by different solvation models.

scholarly journals Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens

ACS Omega ◽  
2020 ◽  
Author(s):  
Kazushi Yamaguchi ◽  
Kohei Otomo ◽  
Yuichi Kozawa ◽  
Motosuke Tsutsumi ◽  
Tomoko Inose ◽  
...  
Keyword(s):  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Photophysical Properties of Protoporphyrin IX, Pyropheophorbide-a, and Photofrin® in Different Conditions

2021 ◽  
Vol 14 (2) ◽  
pp. 138
Author(s):  
Bauyrzhan Myrzakhmetov ◽  
Philippe Arnoux ◽  
Serge Mordon ◽  
Samir Acherar ◽  
Irina Tsoy ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Photophysical Properties ◽  
Protoporphyrin Ix ◽  
Fluorescence Emission ◽  
Solvent Polarity ◽  
Visible Absorption ◽  
Light Dosimetry ◽  
Time Resolved ◽  
Pyropheophorbide A ◽  
Influence Of Solvent

Photodynamic therapy (PDT) is an innovative treatment of malignant or diseased tissues. The effectiveness of PDT depends on light dosimetry, oxygen availability, and properties of the photosensitizer (PS). Depending on the medium, photophysical properties of the PS can change leading to increase or decrease in fluorescence emission and formation of reactive oxygen species (ROS) especially singlet oxygen (1O2). In this study, the influence of solvent polarity, viscosity, concentration, temperature, and pH medium on the photophysical properties of protoporphyrin IX, pyropheophorbide-a, and Photofrin® were investigated by UV-visible absorption, fluorescence emission, singlet oxygen emission, and time-resolved fluorescence spectroscopies.

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