A molecular probe based on pyrimidine imidazole derivatives for stable super-resolution endoplasmic reticulum imaging in living cells

2018 ◽  
Vol 42 (18) ◽  
pp. 14725-14728 ◽  
Author(s):  
Ling Hu ◽  
Sajid Hussain ◽  
Tianyan Liu ◽  
Yuanzhen Yue ◽  
Jiejie Liu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Molecular Probes ◽  
Living Systems ◽  
Molecular Probe ◽  
Imidazole Derivatives ◽  
Two Photon Microscopy ◽  
Two Photon

Multi-functional florescent dyes capable of acting as molecular probes in living systems under two-photon microscopy, as well as super-resolution nanoscopy, are of great interest.

scholarly journals A Simple Probe for Super-Resolution Imaging of the Endoplasmic Reticulum in Living Cells

2018 ◽  
Vol 101 (11) ◽  
pp. e1800165 ◽  
Author(s):  
Elias A. Halabi ◽  
Salome Püntener ◽  
Pablo Rivera-Fuentes
Keyword(s):  
Endoplasmic Reticulum ◽  
Super Resolution ◽  
Living Cells ◽  
Resolution Imaging

scholarly journals Polysulfide-triggered fluorescent indicator suitable for super-resolution microscopy and application in imaging

2018 ◽  
Vol 54 (30) ◽  
pp. 3735-3738 ◽  
Author(s):  
Anila Hoskere A. ◽  
Sreejesh Sreedharan ◽  
Firoj Ali ◽  
Carl G. Smythe ◽  
Jim A. Thomas ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Membrane ◽  
Super Resolution ◽  
Molecular Probe ◽  
Fluorescent Indicator ◽  
Super Resolution Microscopy

A new physiologically benign and cell membrane permeable BODIPY based molecular probe, MB-Sn, specifically senses intracellular hydrogen polysulfides (H2Sn, n > 1) localized in the endoplasmic reticulum.

A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

2018 ◽  
Vol 10 (47) ◽  
pp. 5702-5706 ◽  
Author(s):  
Nan Zhang ◽  
Baoli Dong ◽  
Xiuqi Kong ◽  
Wenhui Song ◽  
Weiying Lin
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Ph Changes ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

The pH changes of the endoplasmic reticulum (ER) are closely related to many diseases.

A dual-site two-photon fluorescent probe for visualizing mitochondrial aminothiols in living cells and mouse liver tissues

2016 ◽  
Vol 40 (9) ◽  
pp. 7399-7406 ◽  
Author(s):  
Fangfang Meng ◽  
Yong Liu ◽  
Xiaoqiang Yu ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Mouse Liver ◽  
Living Cells ◽  
Living Systems ◽  
Two Photon ◽  
Long Period ◽  
First Time ◽  
Dual Site ◽  
Liver Tissues

In this work, we developed a dual-site two-photon (TP) fluorescent RSH probe (CA-TPP) for imaging mitochondrial RSH in living systems. In particular, probe CA-TPP was capable of using TP fluorescence to track mitochondrial RSH over a long period for the first time.

Tracking mitochondrial viscosity in living systems based on a two-photon and near red probe

2019 ◽  
Vol 43 (43) ◽  
pp. 16945-16949 ◽  
Author(s):  
Min Peng ◽  
Junling Yin ◽  
Weiying Lin
Keyword(s):  
Fluorescence Probe ◽  
Living Cells ◽  
Living Systems ◽  
Red Emission ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Viscosity Changes

A two-photon fluorescence probe, Mito-V, with near-red emission to measure the viscosity changes of mitochondria in living cells and in vivo.

Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

2019 ◽  
Vol 11 (35) ◽  
pp. 4450-4455 ◽  
Author(s):  
Wenhui Song ◽  
Baoli Dong ◽  
Yaru Lu ◽  
Xiuqi Kong ◽  
Abdul Hadi Mehmood ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Dysfunction ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

As an important reactive oxygen species, hypochlorous acid (HClO) could irreversibly destroy Ca2+-ATPase to inhibit Ca2+ transport in the endoplasmic reticulum (ER), which may eventually lead to cell dysfunction and cell death.

A molecular rotor sensor for detecting mitochondrial viscosity in apoptotic cells by two-photon fluorescence lifetime imaging

2020 ◽  
Vol 44 (26) ◽  
pp. 11342-11348 ◽  
Author(s):  
Ming-Xuan Hou ◽  
Liu-Yi Liu ◽  
Kang-Nan Wang ◽  
Xi-Juan Chao ◽  
Rong-Xue Liu ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Living Cells ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Molecular Rotor ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A two-photon fluorescent probe was developed for detecting mitochondrial viscosity during apoptosis of living cells by two-photon microscopy (TPM) and fluorescence lifetime imaging microscopy (FLIM) with good selectivity and highly biocompatible.

Ratiometric fluorescent sensors for detecting zinc ions in aqueous solution and living cells with two-photon microscopy

2011 ◽  
Vol 156 (1) ◽  
pp. 410-415 ◽  
Author(s):  
Lin Xue ◽  
Zhangjian Fang ◽  
Guoping Li ◽  
Huanhuan Wang ◽  
Hua Jiang
Keyword(s):  
Aqueous Solution ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Zinc Ions ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Benzo[g]coumarin-Based Fluorescent Probes for Bioimaging Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuna Jung ◽  
Junyang Jung ◽  
Youngbuhm Huh ◽  
Dokyoung Kim
Keyword(s):  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Molecular Probes ◽  
Important Species ◽  
Disease Biomarkers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Photophysical Parameters ◽  
Absorbing Property ◽  
Linear Shape

Benzo[g]coumarins, which consist of coumarins fused with other aromatic units in the linear shape, have recently emerged as an interesting fluorophore in the bioimaging research. The pi-extended skeleton with the presence of electron-donating and electron-withdrawing substituents from the parent coumarins changes the basic photophysical parameters such as absorption and fluorescence emission significantly. Most of the benzo[g]coumarin analogues show red/far-red fluorescence emission with high two-photon absorbing property that can be applicable for the two-photon microscopy (TPM) imaging. In this review, we summarized the recently developed benzo[g]coumarin analogues including photophysical properties, synthesis, and applications for molecular probes that can sense biologically important species such as metal ions, cell organs, reactive species, and disease biomarkers.

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