scholarly journals Lanthanide nano-drums: a new class of molecular nanoparticles for potential biomedical applications

2014 ◽  
Vol 175 ◽  
pp. 241-255 ◽  
Author(s):  
Richard A. Jones ◽  
Annie J. Gnanam ◽  
Jonathan F. Arambula ◽  
Jessica N. Jones ◽  
Jagannath Swaminathan ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Biomedical Applications ◽  
Near Infrared ◽  
Photophysical Properties ◽  
Diagnostic Systems ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Self Assembling ◽  
Molecular Nanoparticles

We are developing a new class of lanthanide-based self-assembling molecular nanoparticles as potential reporter molecules for imaging, and as multi-functional nanoprobes or nanosensors in diagnostic systems. These lanthanide “nano-drums” are homogeneous 4d–4f clusters approximately 25 to 30 Å in diameter that can emit from the visible to near-infrared (NIR) wavelengths. Here, we present syntheses, crystal structures, photophysical properties, and comparative cytotoxicity data for six nano-drums containing either Eu, Tb, Lu, Er, Yb or Ho. Imaging capabilities of these nano-drums are demonstrated using epifluorescence, total internal reflection fluorescence (TIRF), and two-photon microscopy. We discuss how these molecular nanoparticles can to be adapted for a range of assays, particularly by taking advantage of functionalization strategies with chemical moieties to enable conjugation to protein or nucleic acids.

scholarly journals Dual Near Infrared Two-Photon Microscopy for Deep-Tissue Dopamine Nanosensor Imaging

2017 ◽  
Author(s):  
Jackson T. Del Bonis-O’Donnell ◽  
Ralph H. Page ◽  
Abraham G. Beyene ◽  
Eric G. Tindall ◽  
Ian McFarlane ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photon Absorption ◽  
Deep Tissue ◽  
Biologically Relevant ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Tissue Phantom ◽  
Photon Excitation ◽  
Photon Absorption And Scattering ◽  
Two Photon Excitation

A key limitation for achieving deep imaging in biological structures lies in photon absorption and scattering leading to attenuation of fluorescence. In particular, neurotransmitter imaging is challenging in the biologically-relevant context of the intact brain, for which photons must traverse the cranium, skin and bone. Thus, fluorescence imaging is limited to the surface cortical layers of the brain, only achievable with craniotomy. Herein, we describe optimal excitation and emission wavelengths for through-cranium imaging, and demonstrate that near-infrared emissive nanosensors can be photoexcited using a two-photon 1560 nm excitation source. Dopamine-sensitive nanosensors can undergo two-photon excitation, and provide chirality-dependent responses selective for dopamine with fluorescent turn-on responses varying between 20% and 350%. We further calculate the two-photon absorption cross-section and quantum yield of dopamine nanosensors, and confirm a two-photon power law relationship for the nanosensor excitation process. Finally, we show improved image quality of the nanosensors embedded 2 mm deep into a brain-mimetic tissue phantom, whereby one-photon excitation yields 42% scattering, in contrast to 4% scattering when the same object is imaged under two-photon excitation. Our approach overcomes traditional limitations in deep-tissue fluorescence microscopy, and can enable neurotransmitter imaging in the biologically-relevant milieu of the intact and living brain.

scholarly journals Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn
Keyword(s):  
Wavelength Region ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
New Class ◽  
Two Photon ◽  
Deep Tissue Imaging

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

Wide-field two-photon microscopy: features and advantages for biomedical applications

2007 ◽  
Author(s):  
S. Wachsmann-Hogiu ◽  
J. Y. Hwang ◽  
E. Lindsley ◽  
D. L. Farkas
Keyword(s):  
Biomedical Applications ◽  
Wide Field ◽  
Two Photon Microscopy ◽  
Two Photon

Aggregation-Induced Emission Luminogen with Near-Infrared-II Excitation and Near-Infrared-I Emission for Ultradeep Intravital Two-Photon Microscopy

ACS Nano ◽  
2018 ◽  
Vol 12 (8) ◽  
pp. 7936-7945 ◽  
Author(s):  
Ji Qi ◽  
Chaowei Sun ◽  
Dongyu Li ◽  
Hequn Zhang ◽  
Wenbin Yu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Aggregation Induced Emission ◽  
Two Photon Microscopy ◽  
Two Photon

Analogs of Michler’s ketone for two-photon absorption initiation of polymerization in the near infrared: synthesis and photophysical properties

2006 ◽  
Vol 30 (11) ◽  
pp. 1606-1613 ◽  
Author(s):  
Gilles Lemercier ◽  
Cécile Martineau ◽  
Jean-Christophe Mulatier ◽  
Irène Wang ◽  
Olivier Stéphan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Photophysical Properties ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Initiation Of Polymerization

scholarly journals Synthesis and Photoswitching Properties of Bioinspired Dissymmetric 2,6-Gamma-Pyrone, Analogue of Cyclocurcumin

2021 ◽  
Author(s):  
Jeremy Pecourneau ◽  
Raul Losantos ◽  
Antonio Monari ◽  
Stephane Parant ◽  
Andreea Pasc ◽  
...  
Keyword(s):  
Near Infrared ◽  
Uv Light ◽  
Photophysical Properties ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Order Of Magnitude ◽  
Linear Optical Properties ◽  
Natural Counterpart ◽  
Therapeutic Properties

Cyclocurcumin, a turmeric curcuminoid with potential therapeutic properties, is also a natural photoswitch that may undergo E/Z photoisomerization under UV light. In order to be further exploited in relevant biological applications, photoactivation under near infrared (NIR) irradiation is required. Such requirement can be met through opportune chemical modifications,and most notably by favoring two-photon absorption (TPA) probability. Herein, a general and efficient synthesis of a biomimetic 2,6-g-pyrone analogue of cyclocurcumin is described, motivated by the fact that molecular modeling previews an order of magnitude increase of the NIR TPA cross-section for the latter compared to the natural counterpart. Three retrosynthetic pathways have been identified (i) via an aryl-oxazole intermediate or via an aryl-diynone through (ii) a bottom-up or (iii) a top-down approach. While avoiding the passage through unstable synthons or low yield intermediate reactions, only the latest approach could conveniently afford the 2,6-g-pyrone analogue of cyclocurcumin, in ten steps and with an overall yield of 18%. The photophysical properties of our biomimetic analogue have also been characterized showing an improved photo-isomerization yield over the parent natural compound. The potentially improved non-linear optical properties, as well an enhanced stability, may be correlated to the enforcement of the planarity of the pyrone moiety leading to a quadrupolar D-p-A-p-D system.<br>

scholarly journals Dual Near-Infrared Two-Photon Microscopy for Deep-Tissue Dopamine Nanosensor Imaging

2017 ◽  
Vol 27 (39) ◽  
pp. 1702112 ◽  
Author(s):  
Jackson T. Del Bonis-O'Donnell ◽  
Ralph H. Page ◽  
Abraham G. Beyene ◽  
Eric G. Tindall ◽  
Ian R. McFarlane ◽  
...  
Keyword(s):  
Near Infrared ◽  
Deep Tissue ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Benzo[g]coumarin-Based Fluorescent Probes for Bioimaging Applications

2018 ◽  
Vol 2018 ◽  
pp. 1-11 ◽  
Author(s):  
Yuna Jung ◽  
Junyang Jung ◽  
Youngbuhm Huh ◽  
Dokyoung Kim
Keyword(s):  
Photophysical Properties ◽  
Fluorescence Emission ◽  
Molecular Probes ◽  
Important Species ◽  
Disease Biomarkers ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Photophysical Parameters ◽  
Absorbing Property ◽  
Linear Shape

Benzo[g]coumarins, which consist of coumarins fused with other aromatic units in the linear shape, have recently emerged as an interesting fluorophore in the bioimaging research. The pi-extended skeleton with the presence of electron-donating and electron-withdrawing substituents from the parent coumarins changes the basic photophysical parameters such as absorption and fluorescence emission significantly. Most of the benzo[g]coumarin analogues show red/far-red fluorescence emission with high two-photon absorbing property that can be applicable for the two-photon microscopy (TPM) imaging. In this review, we summarized the recently developed benzo[g]coumarin analogues including photophysical properties, synthesis, and applications for molecular probes that can sense biologically important species such as metal ions, cell organs, reactive species, and disease biomarkers.

scholarly journals Synthesis and photoswitching properties of bioinspired dissymmetric gamma-pyrone, analogue of cyclocurcumin

2021 ◽  
Author(s):  
Jeremy Pecourneau ◽  
Raul Losantos ◽  
Antonio Monari ◽  
Stephane Parant ◽  
Andreea Pasc ◽  
...  
Keyword(s):  
Near Infrared ◽  
Uv Light ◽  
Photophysical Properties ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Order Of Magnitude ◽  
Linear Optical Properties ◽  
Natural Counterpart ◽  
Therapeutic Properties

Cyclocurcumin, a turmeric curcuminoid with potential therapeutic properties, is also a natural photoswitch that may undergo E/Z photoisomerization under UV light. In order to be further exploited in relevant biological applications, photoactivation under near infrared (NIR) irradiation is required. Such requirement can be met through opportune chemical modifications,and most notably by favoring two-photon absorption (TPA) probability. Herein, a general and efficient synthesis of a biomimetic 2,6-g-pyrone analogue of cyclocurcumin is described, motivated by the fact that molecular modeling previews an order of magnitude increase of the NIR TPA cross-section for the latter compared to the natural counterpart. Three retrosynthetic pathways have been identified (i) via an aryl-oxazole intermediate or via an aryl-diynone through (ii) a bottom-up or (iii) a top-down approach. While avoiding the passage through unstable synthons or low yield intermediate reactions, only the latest approach could conveniently afford the 2,6-g-pyrone analogue of cyclocurcumin, in ten steps and with an overall yield of 18%. The photophysical properties of our biomimetic analogue have also been characterized showing an improved photo-isomerization yield over the parent natural compound. The potentially improved non-linear optical properties, as well an enhanced stability, may be correlated to the enforcement of the planarity of the pyrone moiety leading to a quadrupolar D-p-A-p-D system.<br>

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