scholarly journals Advanced Analytical Methods for Phenolics in Fruits

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Nesibe Ebru Kafkas ◽  
Müberra Kosar ◽  
Ayşe Tülin Öz ◽  
Alyson E. Mitchell

Phenolic compounds are a group of secondary plant metabolites, many with health-promoting properties that are present in all parts of plants. They have an aromatic structure, including either one or more hydroxyl groups giving them the ability to stabilize free radicals and protect biological tissues against damage related to reactive oxygen species. Phenolic compounds are concentrated in the fruit of plants, and therefore, the fruit can be an important dietary source of these phytochemicals, which exist as monomers, or bound to one another. Polyphenolic compounds are classified into different subclasses based upon the number of phenol ring systems that they contain, saturation, and length of the carbon chain that bind the rings to one another. The phenolic acids present in fruit tissues protect the plant against disease, infections, UV radiation, and insect damage. For this reason, the beneficiary effects of phenolic compounds are continually being investigated for their health-promoting properties and for meeting increased consumer demand for healthy nutritious food. Due to the functional properties of polyphenolic compounds, there is increased interest on improving extraction, separation, and quantification techniques of these valuable bioactive compounds, so they can be used as value-added ingredients in foods, pharmaceuticals, and cosmetics. This review provides information on the most advanced methods available for the analysis of phenolics in fruits.

2020 ◽  
Vol 21 (17) ◽  
pp. 6142
Author(s):  
Daniel A. Jacobo-Velázquez ◽  
Luis Cisneros-Zevallos

Phenolic compounds are secondary plant metabolites with remarkable health-promoting properties [...]


2021 ◽  
Vol 12 ◽  
Author(s):  
Nadja Förster ◽  
Kyriaki Antoniadou ◽  
Matthias Zander ◽  
Sebastian Baur ◽  
Verena Karolin Mittermeier-Kleßinger ◽  
...  

Willow bark is traditionally used for pharmaceutical purposes. Evaluation is so far based on the salicylate content, however, health promoting effects of extracts might be attributed to the interaction of those salicylates with other compounds, which support and complement their action. So far, only S. purpurea, S. daphnoides, and S. fragilis are included in pharmaceutical extracts. Crossing with other species could result in a more diverse secondary metabolite profile with higher pharmacological value. With the help of targeted inter- and intraspecific crossing, new chemotypes were generated, whereby nine different Salix genotypes (S. alba, S. daphnoides, S. humboldtiana, S. lasiandra, S. nigra, S. pentandra, S. purpurea, S. x rubens, S. viminalis) were included in the study. Based on substances known for their health promoting potential and characteristic for Salix (selected phenolic compounds including salicylates), a targeted metabolomics analysis and clustering of 92 generated Salix clones was performed revealing four different cluster/chemoprofiles. In more specific, one group is formed by S. daphnoides clones and inter- and intraspecific hybrids, a second group by S. viminalis clones and inter- and intraspecific hybrids, a third group generally formed by S. alba, S. pentandra, S. x rubens, and S. lasiandra clones and hybrids, and a fourth group by S. purpurea clones and inter- and intraspecific hybrids. Clustering on the basis of the selected phenolic compounds can be used for identifying Salix clones with a different compound profile. New combinations of secondary plant metabolites offer the chance to identify Salix crosses with improved effects on human health.


2003 ◽  
Vol 73 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Andlauer ◽  
Stumpf ◽  
Hubert ◽  
Rings ◽  
Fürst

Phenolic compounds are secondary plant metabolites which have long been associated with flavor and color characteristics of fruits and vegetables. These phenolic compounds attract great interest due to their postulated health protecting properties. However, adequate intakes and absorption rate of phenolic compounds are necessary for these beneficial effects. Until now, little is known about alterations of phenolic compounds content by the cooking process. In the present study, the influence of different volumes of cooking water on the amount of selected phenolic marker compounds resting in the vegetables was assessed. In zucchini, rutin was quantified as a marker for flavonoid glycosides. Chlorogenic acid, representative of phenolic acids was analyzed in carrots. In beans, rutin and quercitrin, both belonging to flavonoid glycosides, were investigated. In potatoes, chlorogenic and caffeic acid were determined. The cooking of zucchini, beans and carrots with smaller amounts of water resulted in significant higher content of phenolic phytochemicals in the vegetables compared to cooking with larger water volumes. For potatoes, which showed great variations in content of phenolic acids after cooking, no significant differences in phenolic acids was observed. It can be concluded from these observations, that real intakes of phenolic compounds from cooked vegetables are lower and that the amounts consumed are therefore overestimated.


Chemija ◽  
2018 ◽  
Vol 29 (4) ◽  
Author(s):  
Ingrīda Augšpole ◽  
Māra Dūma ◽  
Ingmārs Cinkmanis ◽  
Baiba Ozola

Phenolic compounds are of a considerable interest and have received more and more attention in recent years due to their bioactive functions. These components are known as secondary plant metabolites and also possess antimicrobial, antiviral and anti-inflammatory properties. The popular Latvian herbals – Calendula (Calendula officinalis L.), Lady’s-mantle (Alchemilla vulgaris L.), Yarrow (Achillea millefolium L.), Peppermint (Mentha × piperita L.) and Bellis (Bellis perennis L.) – are widely used for herbal teas. The objective of this study was to determine individual phenolic compounds in herbal infusions using high-performance liquid chromatography. The obtained results showed significant differences (p ˂ 0.05) of the phenolics content in the analysed herbal teas. The total content of the identified 14 phenolic compounds in Yarrow tea was on the average 136.76 ± 0.8 mg 100 g–1, in Peppermint tea 304.38 ± 10.7 mg 100 g–1, in Lady’s-mantle tea 319.53 ± 12.5 mg 100 g–1, in Calendula tea 586.36 ± 17.6 mg 100 g–1, but in Bellis tea it was 802.96 ± 21.3 mg 100 g–1. The dominant phenolic compound in Peppermint, Lady’s-mantle and Yarrow teas was rutin, but chlorogenic acid in Bellis tea. Calendula tea has an abundant quantity of sinapic acid.


Proceedings ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 19 ◽  
Author(s):  
Christine Fuchs ◽  
Tamara Bakuradze ◽  
Elke Richling

Winery by-products, including pomace, stem, vine leaves and shoots, arise in high amounts during the wine-making process. Due to high contents of secondary plant metabolites, such as polyphenols, their usage in the food, cosmetics and pharmaceutical industry is possible. In this study, we investigated effects of an extract prepared from vine shoots (Vitis vinifera L. cv. Riesling) on riboflavin-induced DNA damage in human hepatocellular carcinoma cells HepG2 by comet assay. Significant decreases of DNA damages were detected after 2 h and 24 h extract incubation at concentration ranges of 1 to 30 µg/mL (2 h) and 1 to 10 µg/mL (24 h), respectively. These effects may be attributable to polyphenolic compounds, which has to be further investigated.


2021 ◽  
Vol 16 (5) ◽  
pp. 1934578X2110150
Author(s):  
Nam Su Kim ◽  
Jae Kwang Kim ◽  
Ramaraj Sathasivam ◽  
Hong Woo Park ◽  
Bao Van Nguyen ◽  
...  

It has been assumed that abiotic stresses often lead to osmotic and ionic stress in plants either inducing or reducing secondary plant metabolites. Therefore, the influence of NaCl, glycinebetaine (betaine), and NaCl with betaine on the growth and variation in the accumulation of phenolic compounds was investigated in safflower ( Carthamus tinctorius L.). The growth pattern of safflower sprouts was significantly influenced by these treatments. It was found that with increases in the concentration of NaCl, all growth parameters steadily decreased, but growth was markedly increased by adding different concentrations of betaine, especially at 0.5 mM, which produced the highest growth in terms of different growth parameters. High-performance liquid chromatographic (HPLC) analysis revealed changes in 7 different phenolic compounds in response to different treatments. After treatment with up to 200 mM NaCl, the levels of catechin, ferulic acid, benzoic acid, and kaempferol increased, whereas the levels of the remaining phenolic compounds, especially chlorogenic acid, and p-coumaric acid were reduced. Our results suggest that the growth suppression due to salinity stress is decreased in the sprouts of safflower by adding betaine.


Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 915
Author(s):  
Olga Babich ◽  
Stanislav Sukhikh ◽  
Artem Pungin ◽  
Lidiya Astahova ◽  
Evgeny Chupakhin ◽  
...  

The cultivation of plants in the form of callus cultures constitutes a renewable source of secondary plant metabolites. The conditions for the cultivation of callus cultures affect the yield of target compounds. Callus cultures of Hyssopus officinalis were chosen for study. Nutrient media of various compositions were used for Hyssopus officinalis callus culture. For each culture, data on the quantitative contents of saponins, flavonoids and polyphenolic compounds, as well as antioxidant activity, were obtained. It was found that Murashige and Skoog medium supplemented with 1-naphthylacetic acid and kinetin led to the highest yield of secondary metabolites.


2021 ◽  
Author(s):  
D. Tomenko ◽  
E. Aksenov ◽  
Lyudmila Novikova

The paper discusses the classification, structure and properties of natural phenolic compounds found in conifers wood species of Russia. The reasons for the variety of detected phenolic compounds (more than 2000) are considered, including the type and conditions of plant growth, environmental factors, as well as methods for extraction of substances. Coniferous extractives include monomeric, dimeric and polymeric phenolic compounds in bound and free form, and their content differs significantly for various species and parts of a woody plant. Depending on the polarity of the solvent used (water, petroleum ether, dimethyl ether, ethyl acetate, acetone, etc.), the yield, chemical composition and structure of the extracted phenolic compound change. It was shown that bark extracts of Larch and Fir contain the most phenolic acids and extractive substances than Pine, Cedar and Spruce, while the content of polar substances is higher in needles, and non- polar substances in plant shoots. Phenolic compounds are secondary plant metabolites, exhibiting fungicidal, virucidal and strong antioxidant effects, that make them a valuable basis for the creation of drugs.


Author(s):  
Annalene Grace E. Co

Medicinal plants have bioactive components which are used for curing various human diseases. They also play an essential role in healing. Fresh fruits and leaves of collected plant samples were used following standard solvent extraction protocol and qualitative analysis to detect secondary plant metabolites. The ethanolic extracts of eight indigenous medicinal plants of Quirino Province, Cagayan Valley, Philippines, were subjected to phytochemical analysis. Results confirmed alkaloids, saponins, phytosterols, phenolic compounds, and flavonoids in specific extracts. The inferences of the findings are discussed in light of the role of indigenous plants in the traditional medicinal system.


2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Yassine Ez zoubi ◽  
Mouhcine Fadil ◽  
Dalila Bousta ◽  
Abdelhakim El Ouali Lalami ◽  
Mohammed Lachkar ◽  
...  

Secondary plant metabolites, in particular phenolic compounds, are characterized by great diversity in the plant kingdom and are widely used in the medical and industrial fields. The extraction of these compounds represents a critical step, and the differences in extraction conditions strongly affect the yields and the total concentrations of polyphenols in the extracts. In this way, the objective of the present study was to optimize the extraction parameters of the polyphenols from Lavandula stoechas using the extraction technique assisted by ultrasound. Yield and the total concentration of polyphenols have been optimized, taking into account three variables, the extraction time (min), the ethanol concentration (%), and the solvent/extract ratio (ml/g). The optimum extraction yield (31.88%) was obtained by ensuring the following parameters: an ethanol concentration of 40%, a liquid/solid ratio of 30 ml/g, and a time processing of 32.62 min. The maximum concentration of total polyphenols (190.14 mg gallic acid equivalents (GAE)/g) was obtained after 21.5 min of extraction, with a liquid/solid ratio of 30 ml/g and a concentration of ethanol at 40%. In order to obtain the maximum yield (24.9%) and the total concentration of polyphenols (190.14 mg GAE/g) simultaneously, the following parameters must be adjusted: an extraction time of 21.5 min, a liquid/solid ratio of 30 ml/g, and a concentration of ethanol at 40%. The experimental values of the yield and the total concentration of the polyphenols were in good agreement with the predicted values, which suggests that the ultrasonic extraction model adopted in this study is validated.


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