“Green technology” processing of pine (Pinus sylvestris L.) and larch (Larix sibirica Ledeb.) wood greenery to produce bioactive extracts

In order to explore the extractives of conifers an effective and environmentally friendly method of extraction with aqueous-alkaline solution allowing to isolate up to 10.4% of extractive substances (ES) from pine wood greenery and up to 6.9% from larch wood greenery was investigated. The component fractional composition of aqueous-alkaline extracts was studied. The antioxidant fraction activity of neutral and acidic components isolated from the produced extracts was evaluated. It was found that these fractions have a high antioxidant activity, where the activity of larch extract components was higher than that of the respective components of pine extract. Due to their rich chemical composition and high biological activity, extracts of pine and larch wood greenery produced by aqueous-alkaline extraction have good application prospects as biologically active preparations.

Evaluation of the biochemical composition of corn grain of the selection of FGBNU RosNIISK "Rossorgo" for further use in the agro-industrial complex

The article examines the biological value of varieties, hybrids (hybrid populations) and lines of maize selection of the Federal State Budgetary Scientific Institution RosNIISK "Rossorgo" in the framework of the biochemical composition of grain. The main indicators were studied: protein, fat, ash, fiber, starch, and nitrogen-free extractive substances. The maximum protein value of 13.58% was in the RSK 3 maize line. The largest number of mineral substances (1.79%) was also noted here. The highest amount of fat (7.50%) and starch (69.24%) was in the Zuckerka variety. A conclusion was made about the possibility of further selection for the breeding of forms with directional properties and biological value. Keywords: CORN, VARIETIES, BIOLOGICAL VALUE, PROTEIN, FAT, FIBER, MINERAL SUBSTANCES, STARCH

COMPOSITION OF BIOLOGICALLY ACTIVE SUBSTANCES AND ANTIRADICAL ACTIVITY OF EXTRACTS FROM FIVE SPECIES OF PLANTS OF THE ASPARAGACEAE FAMILY

Many members of the Asparagaceae family are used in traditional medicine in different countries and characterized by a high content of biologically active metabolites. In this work, the qualitative composition and quantitative content of the components of methanol extracts from leaves and underground organs of Sansevieria cylindrica Bojer ex Hook, Sansevieria trifasciata Prain, Polianthes tuberosa L., leaves of Yucca filamentosa L. and Furcraea gigantea var. watsoniana (Hort. Sander) Drumm. were determined. Extraction of plant leaves and underground organs using 80% methanol resulted in 5.2–16.7% and 16–25.1% of the total extractive substances consequently. The presence of steroidal saponins in the extracts was shown by thin layer chromatography. Spirostanol saponins were predominate in the extracts from leaves of Y. filamentosa, F. gigantea and underground organs of S. cylindrica, S. trifasciata, P. tuberosa, furastanol saponins – in the extracts from leaves of S. cylindrica and S. trifasciata. The content of terpenoid and phenolic compounds in the extracts established using spectrophotometry significantly differs depending on the plant species and their anatomical part. All the extracts tested exhibited inhibition of the 2,2-diphenyl-1-picrylhydrazyl free radical in dose-dependent manner. The highest antiradical activity demonstrated the extract from the leaves of Y. filamentosa (IC50 = 25.95 μg/ml) containing the largest amount of phenolic compounds, including flavonoids – 51.3 and 15.5% of the total extractive substances.

PROSPECTS FOR OBTAINING FOOD ADDITIVES FROM LEAVES AND FLOWERS

Использование продуктов переработки зеленой массы базилика как природного консерванта позволяет придавать пище оригинальный вкус и аромат, продлять срок ее хранения. Оценены перспективы получения пищевых добавок из листьев и соцветий базилика эвгенольного и базилика обыкновенного. Применен комплексный подход к переработке сырья, включающий получение эфирного масла, сухих пищевых добавок, СО2-экстракта. Исследован фракционный состав эфирного масла паровой перегонки из свежего сырья базилика эвгенольного и базилика обыкновенного с выходом 0,45 и 0,32% соответственно. Выход сухой пищевой добавки из свежего сырья базилика эвгенольного при мягких температурных режимах составил 9,25%, а базилика обыкновенного – 10,5%. Модернизирована экстракционная установка для получения СО2-экстрактов за счет внедрения пилотных устройств, позволяющих предварительно оценивать содержание экстрактивных веществ в поступающем сырье, а также устройств подготовки хладоагента и теплоносителя. Установлены режимы работы СО2-экстракционной установки: рабочее давление 5464–6289 кПа, температура процесса экстракции 18–24°С, продолжительность обработки одной загрузки сырья 4–4,5 ч. Выход СО2-экстракта из сухого базилика эвгенольного составил 3,2%, а из базилика обыкновенного – 2,8%. Даны рекомендации по использованию продуктов переработки растительного сырья базилика в качестве натуральных пищевых добавок. The use of basil green mass processing products as a natural preservative allows you to give food an original taste and aroma, prolong its shelf life. The prospects of obtaining food additives from the leaves and inflorescences of eugenol basil and basil vulgaris are evaluated. A comprehensive approach to the processing of raw materials has been applied, including the production of essential oil, dry food additives, and CO2-extract. The fractional composition of steam-distilled essential oil from fresh raw materials of eugenolic basil and ordinary basil with a yield of 0.45 and 0.32% respectively investigated. The yield of a dry food additive from fresh raw materials of eugenolic basil under mild temperature conditions was 9,25%, and ordinary basil – 10,5%. The extraction plant for producing CO2-extracts has been upgraded through the introduction of pilot devices that allow preliminary assessment of the content of extractive substances in incoming raw materials, as well as devices for the preparation of refrigerant and coolant. The modes of operation of the CO2-extraction plant are set: operating pressure 5464–6289 kPa, extraction process temperature 18–24°C, processing time of one load of raw materials 4–4,5 hours. The yield of CO2-extract from dry eugenolic basil was 3,2%, and from ordinary basil – 2,8%. Recommendations on the use of basil vegetable raw materials processing products as natural food additives are given.

Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.

Fertilizers, plant density and nutritional properties of corn grain

The article presents data on the influence of an increasing plant density of an early-ripening corn hybrid on the biochemical composition and fodder properties of grain, depending on the level of mineral nutrition. It is shown that the highest protein supply was noted for the grain obtained in the variants with nitrogen feeding; the increase in crude protein in comparison with the variants on the natural agrobackground was 1.56%. The use of complete mineral fertilization had no advantages over a single application of nitrogen-phosphorus fertilizers; against these nutritional backgrounds, the increase of 0.97-1.05% was obtained in relation to the unfertilized agricultural background. As the crops crowd, there is a tendency for the crude protein content in the grain to increase. Over the years of testing, the stable influence of the conditions of mineral nutrition and the plants density on the content of crude fiber, crude ash and nitrogen-free extractive substances has not been established. The grain with a higher crude fat content was obtained with the introduction of complete mineral fertilization, and the crowding of the crops led to the decrease in its content in the grain.

PHENOLIC COMPOUNDS OF CONIFEROUS TREES

The paper discusses the classification, structure and properties of natural phenolic compounds found in conifers wood species of Russia. The reasons for the variety of detected phenolic compounds (more than 2000) are considered, including the type and conditions of plant growth, environmental factors, as well as methods for extraction of substances. Coniferous extractives include monomeric, dimeric and polymeric phenolic compounds in bound and free form, and their content differs significantly for various species and parts of a woody plant. Depending on the polarity of the solvent used (water, petroleum ether, dimethyl ether, ethyl acetate, acetone, etc.), the yield, chemical composition and structure of the extracted phenolic compound change. It was shown that bark extracts of Larch and Fir contain the most phenolic acids and extractive substances than Pine, Cedar and Spruce, while the content of polar substances is higher in needles, and non- polar substances in plant shoots. Phenolic compounds are secondary plant metabolites, exhibiting fungicidal, virucidal and strong antioxidant effects, that make them a valuable basis for the creation of drugs.

Monitoring and assessment of the effect of benzo (a) pyrene for coniferous and deciduous plants in anthropogenically polluted areas

Benzo (a) pyrene content in plants and soils in anthropogenically polluted areas pollution was monitored. The influence of benzo (a) pyrene on conifers and deciduous plants was assessed. In forests with Scots pine (Pinus sylvestris L.) and silver birch (Betula pendula) trunks, the content of lignin, cellulose, and extractive low molecular weight polar substances was determined by the concentration of benzo (a) pyrene and tree species. In forest ecosystems, the background content of carcinogen (1–5 μg / kg) did not affect the content of lignin and cellulose, but stimulated the synthesis of extractive substances, especially in birch. For pine, there was a direct correlation between the concentration of benzo (a) pyrene in two -year-old needles and its peroxidase activity. An increase in the activity of oxidoreductases and their participation in the synthesis of phenolic, tannin and other organic (extractive) substances, depending on the concentration of benzo (a) pyrene, indicate its biostimulating properties. Various physiological and biochemical reactions in woody and herbaceous plants were analyzed depending on the concentration (dose) of benzo (a) pyrene in the composition of plants and in soils polluted by anthropogenic emissions. Opposite reactions of coniferous and leafy trees to benzo (a) pyrene were observed. On the basis of own and published data, the natural, intermediate transitional and technogenic ranges of benzo (a) pyrene concentrations have been identified; among which the effect on plants varies from stimulating to inhibitory.

Mycolysis of wood, its products and their use. IV. Component analysis of mycologically destroyed wood

The article is devoted to the study of the component composition of mycologically destroyed wood. As a result of wood mycolysis under the action of wood-destroying fungi enzymes, so-called «rot» is formed with a chemical composition different from healthy wood. It is noted that the «brown rot» of wood is enriched with lignin components of wood and «white rot» with carbohydrate components of wood. The results of analyses of ligno-carbohydrate complexes of spruce wood «brown rot», birch wood «white rot» and a group study of low-molecular compounds isolated by extraction methods with various solvents are presented. It is noted that the content of lignin in the sample of «brown rot» wood is four times higher than in the sample of «white rot». It is shown that birch wood with «white rot» is significantly enriched with cellulose. The chemical nature of some groups of extractive substances has been revealed. In the extracts of mycologically destroyed wood, substances of phenolic, alcoholic and quinone nature were found. The presence of derivatives of aromatic and aliphatic carbonyl and carboxyl compounds is noted. Among the products of mycolysis, carbohydrates, flavanoids and terpenoids have been identified. Based on the analysis, an assumption is made that mycologically destroyed wood contains a wide range of phenolic and carbohydrate compounds contained both in the original wood and in the spores and hyphae of wood-destroying fungi themselves. The idea is substantiated that the study of extractive substances of mycologically destroyed wood makes it possible to predict possible directions of the prospective use of this raw material for obtaining target products. This article is the fourth in the cycle «Wood mycolysis, its products and their use», the previous ones were published in the journal «Forestry Bulletin», 2020, v. 24, no. 2, 6; v. 25, no. 1.