scholarly journals In Vitro Evaluation of Antioxidant and Antimicrobial Activities of Melaleuca alternifolia Essential Oil

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Xiaofeng Zhang ◽  
Yanjun Guo ◽  
Liying Guo ◽  
Hui Jiang ◽  
Qianhua Ji
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Maximal Effect ◽  
Melaleuca Alternifolia ◽  
Pharmaceutical Industries ◽  
Antioxidant And Antimicrobial Activity

The in vitro antioxidant and antimicrobial activity of the essential oil from Melaleuca alternifolia (M. alternifolia) was evaluated in this report. The antioxidant potential of the essential oil from M. alternifolia was evaluated by the DPPH (2,2-diphenyl-1-picrylhydrazyl) method, thiobarbituric acid reactive species (TBARS) assay, and the hydroxyl radical scavenging activity method. The essential oil from M. alternifolia was able to reduce DPPH with an EC50 (concentration for 50% of maximal effect) of 48.35 μg/ml, inhibit the lipid peroxidation with an IC50 (50% inhibitory concentration) of 135.9 μg/ml, and eliminate hydroxyl radicals with an EC50 of 43.71 μg/ml. Antimicrobial screening, minimum inhibitory concentration, and minimum bactericidal concentration assays showed that the essential oil from M. alternifolia inhibited strongly the growth of different types of microorganisms, including Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Penicillium italicum Wehmer, and Penicillium digitatum Sacc. Thus, the essential oil of M. alternifolia possesses antioxidant and antimicrobial activity and could be suitable for use as a natural preservative ingredient in food, agriculture, and pharmaceutical industries.

scholarly journals Cytotoxic, antioxidant, and antimicrobial activities of Celery (Apium graveolens L.)

2021 ◽  
Vol 17 (1) ◽  
pp. 147-156
Author(s):  
Mohammed Saleh Al Aboody ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antihypertensive Agents ◽  
Apium Graveolens ◽  
Cell Protein ◽  
Dilution Method ◽  
Local Market ◽  
Antioxidant And Antimicrobial Activity

Celery (Apium graveolens Linn, Family: Apiaceae) is a common edible herb used as a spice in the traditional medicine of several nations since time immemorial. The whole plant is extensively used in cooking as soups and salads. A. graveolens has various pharmacological properties such as anticancer, anti-obesity, anti-hepatotoxic, and antihypertensive agents. Hence, it is of interest to document the in vitro cytotoxic, antioxidant, and antimicrobial activity of A. graveolens. The plants were collected in the local market, shade dried, and different parts of the plants were extracted with 70% ethanol using a cold maceration process. Antioxidant tests were performed based on the various radical scavenging methods. Antimicrobial activity and MIC were completed using the respective cup-plate and two-fold serial dilution method. In vitro cytotoxic studies were achieved by the MTT; Sulphorhodamine B assayed total cell protein content. DLA and ESC cells determined the short-term toxicity. The leaf extract exhibited significant antioxidant properties against NO, DPPH, ABTS, LPO, and HPO methods. Thus, potential inhibition against Gram-positive, Gram-negative, and fungal strains within the MIC ranges of 250-500 μg/ml was observed. All the extracts of the plant presented in the study revealed greater cytotoxicity effects against five respective cancer cell lines, L6, Vero, BRL 3A, A-549, L929, and L-929 with the ranging of 443-168.5 μg/ml. Thus, we show that A. graveolens possess a potential cytotoxic, antioxidant, and antimicrobial activity.

Chemical Composition of Essential Oil, Antioxidant, Antidiabetic, Anti-obesity, and Neuroprotective Properties of Prangos gaubae

2017 ◽  
Vol 12 (12) ◽  
pp. 1934578X1701201 ◽  
Author(s):  
Mir Babak Bahadori ◽  
Gokhan Zengin ◽  
Shahram Bahadori ◽  
Filippo Maggi ◽  
Leila Dinparast
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Skin Diseases ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Volatile Components ◽  
Total Antioxidant ◽  
Pharmaceutical Industries

Chemical composition of essential oil and the potential of Prangos gaubae for the management of public health problems such as Alzheimer's disease, obesity, Diabetes mellitus, and skin diseases were evaluated for the first time. In this direction, enzyme inhibitory effects, antioxidant activity, and total bioactive contents of the plant were determined. EO showed high acetylcholinesterase (2.97 mg GEs/g oil), α-amylase (1.35 mmol ACEs/g oil), α-glucosidase (38.84 mmol ACEs/g oil), and lipase (1.59 mmol OEs/g oil) inhibitory activities. Moreover, strong antioxidant effects were observed in antiradical (DPPH and ABTS), reducing power (CUPRAC and FRAP), total antioxidant, and metal chelating assays. Methanol extract exhibited promising DPPH radical scavenging activity (0.47 mmol TEs/g extract) and also high reducing power in CUPRAC (0.89 mmol TEs/g extract) and FRAP (0.52 mmol TEs/g extract) assays. All extracts showed low total flavonoid but high total phenolics content. Furthermore, they exhibited strong skin-care effect in tyrosinase inhibition assay. EO analysis showed the presence of germacrene D (26.7%), caryophyllene oxide (14.3%), ( E)-caryophyllene (13.8%), and spathulenol (11.3%) as the major volatile components. Results indicated that P. gaubae has promising potential for possible uses in food, cosmetic, and pharmaceutical industries due to its valuable phytoconstituents and biological activities.

scholarly journals In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 453 ◽  
Author(s):  
Nhan Trong Le ◽  
Duc Viet Ho ◽  
Tuan Quoc Doan ◽  
Anh Tuan Le ◽  
Ain Raal ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Antiviral Effects ◽  
Scientific Report ◽  
Main Components ◽  
Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

scholarly journals Antioxidant and Antimicrobial Properties of the Essential Oil and Extracts ofZanthoxylum alatumGrown in North-Western Himalaya

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Sanjay Guleria ◽  
A. K. Tiku ◽  
Apurva Koul ◽  
Sahil Gupta ◽  
Gurjinder Singh ◽  
...  
Keyword(s):  
Essential Oil ◽  
Methanolic Extract ◽  
Micrococcus Luteus ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Preparative Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Curvularia Lunata

The essential oil obtained from the fresh leaves ofZanthoxylum alatumwas analysed by gas chromatography/mass spectrometry (GC/MS). Fourteen components were identified, and linalool (30.58%), 2-decanone (20.85%),β-fenchol (9.43%), 2-tridecanone (8.86%),β-phellandrene (5.99%), Sabinene (4.82%), andα-pinene (4.11%) were the main components. The EO and methanolic extract ofZ. alatumexhibited potent antifungal activity againstAlternaria alternata,Alternaria brassicae, andCurvularia lunata. The EO also showed significant antibacterial activity againstBacillus subtilis,Micrococcus luteus,Staphylococcus aureus, andEscherichia coli. Further, antimicrobial constituents of the EO were isolated by bioautography and preparative thin layer chromatography (PTLC) and identified asβ-fenchol and linalool using GC/MS analysis. In addition to this, the free radical scavenging activity and antioxidant potential of EO and methanolic extract/fractions ofZ. alatumwere also investigated usingin vitroassays including scavenging ability against DPPH•, reducing power and chelating ability on Fe2+ions. Our results demonstrate thatZ. alatumcould be used as a resource of antioxidant and antimicrobial compounds which may find applications in food and pesticide industries.

scholarly journals Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

2008 ◽  
Vol 25 (No. 2) ◽  
pp. 81-89 ◽  
Author(s):  
A. Adiguzel ◽  
H. Ozer ◽  
H. Kilic ◽  
B. Cetin
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Activities ◽  
Satureja Hortensis ◽  
Bacteria And Fungi

The present work reports the <i>in vitro</i> antimicrobial activities of the essential oil and methanol extract from <i>Satureja hortensis</i> as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), &gamma;-terpinene (18.56%), carvacrol (13.98%), and <i>p</i>-cymene (8.97). The essential oil of <i>Satureja hortensis</i> exhibited the activity against 25 bacteria, 8 fungi, and a yeast, <i>C. albicans</i>; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 &micro;l/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

scholarly journals In VitroAntimicrobial Activity of Essential Oil ofThymus schimperi,Matricaria chamomilla,Eucalyptus globulus, andRosmarinus officinalis

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Awol Mekonnen ◽  
Berhanu Yitayew ◽  
Alemnesh Tesema ◽  
Solomon Taddese
Keyword(s):  
Antimicrobial Activity ◽  
Minimum Inhibitory Concentration ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibitory Concentration ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Drug Preparation ◽  
Bacteria And Fungi

In this study, thein vitroantimicrobial activities of four plant essential oils (T. schimperi,E. globulus,R. officinalis, andM. Chamomilla) were evaluated against bacteria and fungi. The studies were carried out using agar diffusion method for screening the most effective essential oils and agar dilution to determine minimum inhibitory concentration of the essential oils. Results of this study revealed that essential oils ofT. schimperi,E. globulus, andR. officinaliswere active against bacteria and some fungi. The antimicrobial effect ofM. chamomillawas found to be weaker and did not show any antimicrobial activity. The minimum inhibitory concentration values ofT. schimperiwere<15.75 mg/mL for most of the bacteria and fungi used in this study. The minimum inhibitory concentration values of the other essential oils were in the range of 15.75–36.33 mg/mL against tested bacteria. This study highlighted the antimicrobial activity of the essential oil ofE. globulus,M. chamomilla,T. Schimperi, andR. officinalis. The results indicated thatT. schimperihave shown strong antimicrobial activity which could be potential candidates for preparation of antimicrobial drug preparation.

scholarly journals Antioxidant, Antimicrobial, Antidiabetic and Cytotoxic Activity of Crocus sativus L. Petals

2020 ◽  
Vol 10 (4) ◽  
pp. 1519
Author(s):  
Adil Farooq Wali ◽  
Houda Ahmed Abou Alchamat ◽  
Huda Khaled Hariri ◽  
Bushra Khaled Hariri ◽  
Godfred A. Menezes ◽  
...  
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Cytotoxic Activity ◽  
Inhibitory Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Crocus Sativus ◽  
Antidiabetic Activity ◽  
Ic50 Values ◽  
Crocus Sativus L

The purpose of this research is to examine in vitro antioxidant, antimicrobial, antidiabetic and cytotoxic efficacy of different extracts of Crocus sativus L. petals. Antioxidant activity of extracts was assessed by DPPH and ABTS (2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) assay. Among all extracts, ethanol (SPE) had the maximum radical scavenging activity with IC50 values 86.63 ± 7.53 μg/mL. The antimicrobial activity was determined by the evaluation of the minimum inhibitory concentration using the agar well plate procedure. The most effective extract was SPE with a minimum inhibitory concentration varying between 500 µg/mL, 250 µg/mL, 125 µg/mL, 62.5 µg/mL, 31.25 µg/mL, 15.63 µg/mL. Cytotoxic activity was tested against MDA-MB-231 cell lines using the MTT method whereas, antidiabetic activity was evaluated using an alpha-glucosidase inhibition assay. All extracts were found to have significant antidiabetic activity.

In vitro antioxidant and antimicrobial activity of Cannabis sativa L. cv ʻFutura 75ʼ essential oil

2020 ◽  
pp. 1-5
Author(s):  
Marika Pellegrini ◽  
Sara Palmieri ◽  
Antonella Ricci ◽  
Annalisa Serio ◽  
Antonello Paparella ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Cannabis Sativa ◽  
Antioxidant And Antimicrobial Activity

scholarly journals Antimicrobial, Antioxidant, and Cytotoxic Properties of Vasicine Acetate Synthesized from Vasicine Isolated fromAdhatoda vasicaL.

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
V. Duraipandiyan ◽  
N. A. Al-Dhabi ◽  
C. Balachandran ◽  
S. Ignacimuthu ◽  
C. Sankar ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Cancer Cell Line ◽  
Ethanolic Extract ◽  
Dry Weight ◽  
Zone Of Inhibition ◽  
Lung Adenocarcinoma Cancer ◽  
A549 Lung

Adhatoda vasica(L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the leaves ofA. vasicausing column chromatography. Vasicine acetate was obtained by acetylation of Vasicine. Vasicine acetate exhibited good zone of inhibition against bacteria: 10 mm againstE. aerogenes, 10 mm againstS. epidermidis, and 10 mm againstP. aeruginosa. Vasicine acetate showed minimum inhibitory concentration values against bacteria:M. luteus(125 μg/mL),E. aerogenes(125 μg/mL),S. epidermidis(125 μg/mL), andP. aeruginosa(125 μg/mL). The radical scavenging activity of Vasicine acetate was the maximum at 1000 μg/mL (66.15%). The compound showed prominent cytotoxic activity in vitro against A549 lung adenocarcinoma cancer cell line. Quantification of Vasicine and Vasicine acetate by HPLC-DAD analysis showed their contents to be 0.2293% and 0.0156%, respectively, on dry weight basis of the leaves. Vasicine acetate could be probed further in drug discovery programme.

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