scholarly journals Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yanbo Zhang ◽  
Ruiyan Li ◽  
Wenzheng Wu ◽  
Yun’an Qing ◽  
Xiongfeng Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Cell Adhesion ◽  
Phase Composition ◽  
Cell Morphology ◽  
Solvent Casting ◽  
Water Contact ◽  
Particulate Leaching ◽  
Cell Adhesion And Proliferation ◽  
Better Than

The purpose of this work was to investigate the porous polyetherimide scaffold (P-PEIs) as an alternative biopolymer for bone tissue engineering. The P-PEIs was fabricated via solvent casting and particulate leaching technique. The morphology, phase composition, roughness, hydrophilicity, and biocompatibility of P-PEIs were evaluated and compared with polyetherimide (PEI) and Ti6Al4V disks. P-PEIs showed a biomimetic porous structure with a modulus of 78.95 ± 2.30 MPa. The water contact angle of P-PEIs was 75.4 ± 3.39°, which suggested that P-PEIs had a wettability surface. Moreover, P-PEIs provides a feasible environment for cell adhesion and proliferation. The relative cell adhesion capability and the cell morphology on P-PEIs were better than PEI and Ti6Al4V samples. Furthermore, the MC3T3-E1 cells on P-PEIs showed faster proliferation rate than other groups. It was revealed that the P-PEIs could be a potential material for the application of bone regeneration.

Modified Poly-ε-Caprolactone (PCL) with Phosphorous Containing Compounds for Biomineralization

2007 ◽  
Vol 361-363 ◽  
pp. 451-454 ◽  
Author(s):  
Mervi Puska ◽  
Joni Korventausta ◽  
Allan Aho ◽  
Jukka Seppälä
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Compression Strength ◽  
Chemical Structure ◽  
Fabrication Technique ◽  
Biomimetic Mineralization ◽  
Water Contact ◽  
Structure And Morphology ◽  
Composite Fabrication ◽  
Phosphate Salts

Biodegradable polymers (e.g. poly-ε-caprolactone, PCL) have been studied largely for tissue engineering applications. The aim of this study was to evaluate the composite fabrication technique on PCL modified with the phosphate salts (i.e. NaH2PO4, Na2HPO4, KH2PO4, or K2HPO4) as well as to determine the compression strengths thereof. The chemical structure and morphology of composites were analyzed using FTIR and SEM/EDX. The influence of a plain phosphate salt in different quantities on the hydrophilic properties of PCL was evaluated by measuring the water contact angle. The results of this study indicated that the addition of phosphate salts led to an improvement in compression strength of PCL composites. According to the results of preliminary biomimetic mineralization, Na2HPO4 seems to increase the bioactivity of PCL.

scholarly journals Fabrication of Electrospun Membranes based on Poly(caprolactone) (PCL) and PCL/Chitosan Layer by Layer for Tissue Engineering

2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Choi Yee Foong ◽  
Naznin Sultana
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Water Contact Angle ◽  
Water Uptake ◽  
Solution Concentration ◽  
Layered Composite ◽  
Layer By Layer ◽  
Electrospun Membrane ◽  
Water Contact ◽  
Electrospun Membranes

Recently, in the field of tissue engineering, fabrication of three-dimensional (3D) electrospun scaffold or membrane is much emphasized. In this study, layered composite scaffolds or membranes were fabricated using two biodegradable polymers, polycaprolactone (PCL) and Chitosan layer-by-layer with multilayer electrospinning method. Characterizations of membranes were done using several techniques. Electrospun composite membrane’s surface morphology was examined using a Scanning Electron Microscopy (SEM) and the wettability of the material’s surface was determined using water contact angle measuring measurement (WCA). Water uptake properties of electrospun membrane were also determined. Using optimized solution concentration and electrospinning processing parameters, the composite PCL/Chitosan and PCL layer-by-layer were successfully fabricated. It was observed from SEM that the composite electrospun membranes produced consisted microfibers and nanofibers within single scaffold. The water contact angle for the double-layered composite electrospun membranes was lower than the pure PCL. The double-layered composite membrane also had higher water uptake properties compared to pure PCL scaffold.

scholarly journals Surface Bioactivation of Polyether Ether Ketone (PEEK) by Sulfuric Acid and Piranha Solution: Influence of the Modification Route in Capacity for Inducing Cell Growth

Biomolecules ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1260
Author(s):  
Flavia Suzany Ferreira dos Santos ◽  
Mariana Vieira ◽  
Henrique Nunes da Silva ◽  
Helena Tomás ◽  
Marcus Vinícius Lia Fook
Keyword(s):  
Contact Angle ◽  
Cell Adhesion ◽  
Sulfuric Acid ◽  
Cell Growth ◽  
Sulfuric Acid Treatment ◽  
Piranha Solution ◽  
Polyether Ether Ketone ◽  
Cell Growth And Proliferation ◽  
Contact Angle Analysis ◽  
Cell Adhesion And Proliferation

The aim of this study was to promote bioactivity of the PEEK surface using sulfuric acid and piranha solution. PEEK was functionalized by a sulfuric acid treatment for 90 s and by piranha solution for 60 and 90 s. Chemical modification of the PEEK surface was evaluated by infrared spectroscopy, contact angle analysis, cytotoxicity, cell adhesion and proliferation. The spectroscopy characteristic band associated with sulfonation was observed in all treated samples. PEEK with piranha solution 60 s showed an increase in the intensity of the bands, which was even more significant for the longer treatment (90 s). The introduction of the sulfonic acid functional group reduced the contact angle. In cytotoxicity assays, for all treatments, the number of viable cells was higher when compared to those of untreated PEEK. PEEK treated with sulfuric acid and piranha solution for 60 s were the treatments that showed the highest percentage of cell viability with no statistically significant differences between them. The modified surfaces had a greater capacity for inducing cell growth, indicative of effective cell adhesion and proliferation. The proposed chemical modifications are promising for the functionalization of PEEK-based implants, as they were effective in promoting bioactivation of the PEEK surface and in stimulating cell growth and proliferation.

Effect of PEO on the Hydrophilicity of PLLA Ultrafine Fibers

2012 ◽  
Vol 535-537 ◽  
pp. 2390-2393
Author(s):  
Jia Xu ◽  
Jin Xian Wang ◽  
Xiang Ting Dong ◽  
Gui Xia Liu ◽  
Wen Sheng Yu
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Contact Angle ◽  
Lactic Acid ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Polyethylene Oxide ◽  
Water Contact ◽  
Ultrafine Fibers ◽  
Scanning Electron

The Polyethylene oxide (PEO) / Poly (L-lactic acid) (PLLA) ultrafine blend fibers have been prepared by electrospinning. The hybrid solvent of trichloromethane and ethanol was found to be the co-solvent for electrospinning. The PEO/PLLA blend solutions in various ratios were studied for electrospinning into ultrafine fibers. The morphology of the fibers was shown by scanning electron microscope (SEM). The hydrophilicity of fiber samples was characterized by determining their water contact angle. The spun ultrafine fibers are expected to be used in the native extracellular matrix for tissue engineering.

Plasma Modification of PCL Porous Scaffolds Fabricated by Solvent-Casting/Particulate-Leaching for Tissue Engineering

2014 ◽  
Vol 11 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Francesca Intranuovo ◽  
Roberto Gristina ◽  
Francesco Brun ◽  
Sara Mohammadi ◽  
Giacomo Ceccone ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Plasma Modification ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Particulate Leaching

Aligned Electrospun Polyvinyl Pyrrolidone/Poly ε-Caprolactone Blend Nanofiber Mats for Tissue Engineering

2016 ◽  
Vol 15 (01n02) ◽  
pp. 1650005 ◽  
Author(s):  
Natthan Charernsriwilaiwat ◽  
Theerasak Rojanarata ◽  
Tanasait Ngawhirunpat ◽  
Praneet Opanasopit
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Water Contact Angle ◽  
Human Fibroblasts ◽  
Polyvinyl Pyrrolidone ◽  
Cell Alignment ◽  
Tissue Engineering Scaffolds ◽  
Electrospinning Technique ◽  
Water Contact ◽  
Nanofiber Mats

Electrospun nanofibrous materials are widely used in medical applications such as tissue engineering scaffolds, wound dressing material and drug delivery carriers. For tissue engineering scaffolds, the structure of the nanofiber is similar to extracellular matrix (ECM) which promotes the cell growth and proliferation. In the present study, the aligned nanofiber mats of polyvinyl pyrrolidone (PVP) blended poly [Formula: see text]-caprolactone (PCL) was successfully generated using electrospinning technique. The morphology of PVP/PCL nanofiber mats were characterized by scanning electron microspore (SEM). The chemical and crystalline structure of PVP/PCL nanofiber mats were analyzed using Fourier transform infrared spectroscopy (FTIR) and powder X-ray diffactometer (PXRD). The water contact angle of mats was investigated. Cell culture studies using normal human fibroblasts (NHF) were performed to assess cell morphology, cell alignment and cell proliferation. The results indicated that the fiber were in nanometer range. The PVP/PCL was well dispersed in nanofiber mats and was in amorphous form. The water contact angle of PVP/PCL nanofiber mats was lower than PCL nanofiber mats. The PVP/PCL nanofiber mats exhibited good biocompatibility with NHF cells. In summary, the PVP/PCL nanofiber mats had potential to be used in tissue engineering and regenerative medicine.

scholarly journals Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 935
Author(s):  
Asiyah Esmail ◽  
João R. Pereira ◽  
Chantal Sevrin ◽  
Christian Grandfils ◽  
Ugur Deneb Menda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Solvent Casting ◽  
Elongation At Break ◽  
Particulate Leaching ◽  
Copolymer Poly ◽  
Water Swelling ◽  
Emulsion Templating

Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer’s polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27–49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18–3.35 MPa tensile strength and 0.07–0.11 MPa Young’s Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.

scholarly journals Shear Bond Strength of Ceramic Veneers to Zirconia–Calcium Silicate Cores

Coatings ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1326
Author(s):  
Ting-Yi Chiang ◽  
Chun-Chuan Yang ◽  
Yi-Hsuan Chen ◽  
Min Yan ◽  
Shinn-Jyh Ding
Keyword(s):  
Contact Angle ◽  
Phase Composition ◽  
Surface Morphology ◽  
Bond Strength ◽  
Plasma Treatment ◽  
Calcium Silicate ◽  
Shear Bond Strength ◽  
Oxygen Plasma ◽  
Oxygen Plasma Treatment ◽  
Water Contact

Improving the bond strength of veneering ceramics to ZrO2-based cores remains a challenge. The purpose of this study was to evaluate the shear bond strength of different ZrO2 cores containing calcium silicate (CaSi) to veneering ceramics. Five types of ZrO2-based cores (n = 230) were divided into two groups: with or without oxygen plasma treatment. These were bound to two veneering ceramics (IPS e.max Ceram or VITA VM9). Shear bond strength of veneering ceramics to various cores was measured (n = 10), in addition to phase composition, surface morphology and contact angle of the cores. The results indicated that the plasma treatment had a significant effect on the water contact angle of the ZrO2-based cores, but had little effect on the bond strength. Regardless of plasma treatment, the highest strength value was recorded in the ZrO2 core specimen containing 20 wt % CaSi, when all cores were adhered to VITA VM 9 veneer. When using IPS e.max Ceram veneer, the shear bond strength of the plasma-treated 20 wt % CaSi-containing ZrO2 core was 16.6 ± 0.9 MPa higher than that of VITA In-Ceram YZ core control (13.4 ± 1.0 MPa) (p < 0.05). We conclude that the presence of 20 wt % CaSi in ZrO2 can improve the shear bond strength of zirconia-based cores to veneering ceramic.

scholarly journals Collagen-Coated Poly(lactide-co-glycolide)/Hydroxyapatite Scaffold Incorporated with DGEA Peptide for Synergistic Repair of Skull Defect

2018 ◽  
Author(s):  
Ming Bi ◽  
Hui Han ◽  
Shujun Dong ◽  
Ying Zhang ◽  
Weiguo Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Defects ◽  
Skull Defect ◽  
Large Area ◽  
Collagen Coating ◽  
Potential Strategy ◽  
Hydroxyapatite Scaffold ◽  
Cell Adhesion And Proliferation ◽  
Scaffold Properties

The treatment of large-area bone defects remains a challenge; however, various strategies have been developed to improve the performances of scaffolds in bone tissue engineering. In this study, poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) scaffold was coated with Asp-Gly-Glu-Ala (DGEA)-incorporated collagen for the repair of rat skull defect. Our results indicated that the mechanical strength and hydrophilicity of PLGA/HA scaffold were clearly improved and conducive to cell adhesion and proliferation. The collagen-coated scaffold with DGEA significantly promoted the repair of skull defect. These findings indicated that a combination of collagen coating and DGEA improved scaffold properties for bone regeneration, thereby providing a new potential strategy for scaffold design.

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