Piezoelectric 3-D Fibrous Poly(3-hydroxybutyrate)-Based Scaffolds Ultrasound-Mineralized with Calcium Carbonate for Bone Tissue Engineering: Inorganic Phase Formation, Osteoblast Cell Adhesion, and Proliferation

2019 ◽  
Vol 11 (21) ◽  
pp. 19522-19533 ◽  
Author(s):  
R. V. Chernozem ◽  
M. A. Surmeneva ◽  
S. N. Shkarina ◽  
K. Loza ◽  
M. Epple ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Calcium Carbonate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Phase Formation ◽  
Osteoblast Cell ◽  
Cell Adhesion And Proliferation

scholarly journals 3D-Printed Scaffolds from Alginate/Methyl Cellulose/Trimethyl Chitosan/Silicate Glasses for Bone Tissue Engineering

2021 ◽  
Vol 11 (18) ◽  
pp. 8677
Author(s):  
Maria Fermani ◽  
Varvara Platania ◽  
Rafaela-Maria Kavasi ◽  
Christina Karavasili ◽  
Paola Zgouro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Methyl Cellulose ◽  
Silicate Glasses ◽  
Trimethyl Chitosan ◽  
Collagen Secretion ◽  
3D Printed ◽  
The Stability

Alginate-based hydrogel inks are commonly used in printing due to their biocompatibility, biodegradation, and cell adhesion. In the present work, 3D printing of hydrogels comprising alginate/methyl cellulose (MC)/trimethyl chitosan (TMC) and silicate glasses was investigated. It was found that TMC increased the stability of the scaffolds after immersion in normal saline solution in comparison with alginate/MC 3D constructs. The stability also remained after the incorporation of pure silicate glasses or bioactive glasses. Immersion in simulated body fluid (SBF) resulted in the formation of hydroxyapatite in all samples. Scanning electron microscopy (SEM) analysis revealed a good cell adhesion of pre-osteoblasts on all scaffold compositions, cell viability assessment displayed a proliferation increase up to seven days in culture, and alkaline phosphatase (ALP) activity was similar in all scaffold compositions without significant differences. Total collagen secretion by the pre-osteoblasts after 7 days in culture was significantly higher in scaffolds containing silicate glasses, demonstrating their ability to promote extracellular matrix formation. In conclusion, 3D-printed porous scaffolds based on alginate/methyl cellulose/TMC are promising candidates for bone tissue engineering applications.

scholarly journals Novel Scaffolds Fabricated Using Oleuropein for Bone Tissue Engineering

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hui Fan ◽  
Junfeng Hui ◽  
Zhiguang Duan ◽  
Daidi Fan ◽  
Yu Mi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Mechanical Tests ◽  
Cell Counting ◽  
Cross Linking ◽  
The Cross ◽  
Cell Adhesion And Proliferation

We investigated the feasibility of oleuropein as a cross-linking agent for fabricating three-dimensional (3D) porous composite scaffolds for bone tissue engineering. Human-like collagen (HLC) and nanohydroxyapatite (n-HAp) were used to fabricate the composite scaffold by way of cross-linking. The mechanical tests revealed superior properties for the cross-linked scaffolds compared to the uncross-linked scaffolds. The as-obtained composite scaffold had a 3D porous structure with pores ranging from 120 to 300 μm and a porosity of73.6±2.3%. The cross-linked scaffolds were seeded with MC3T3-E1 Subclone 14 mouse osteoblasts. Fluorescence staining, the Cell Counting Kit-8 (CCK-8) assay, and scanning electron microscopy (SEM) indicated that the scaffolds enhanced cell adhesion and proliferation. Our results indicate the potential of these scaffolds for bone tissue engineering.

Hierarchically Porous Calcium Carbonate Scaffolds for Bone Tissue Engineering

2017 ◽  
Vol 3 (10) ◽  
pp. 2457-2469 ◽  
Author(s):  
Abiy D. Woldetsadik ◽  
Sudhir K. Sharma ◽  
Sachin Khapli ◽  
Ramesh Jagannathan ◽  
Mazin Magzoub
Keyword(s):  
Tissue Engineering ◽  
Calcium Carbonate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Hierarchically Porous ◽  
Porous Calcium Carbonate

scholarly journals Fabrication and In Vitro Evaluation of 3D Printed Porous Polyetherimide Scaffolds for Bone Tissue Engineering

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Xiongfeng Tang ◽  
Yanguo Qin ◽  
Xinyu Xu ◽  
Deming Guo ◽  
Wenli Ye ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Porous Scaffold ◽  
3D Printed

For bone tissue engineering, the porous scaffold should provide a biocompatible environment for cell adhesion, proliferation, and differentiation and match the mechanical properties of native bone tissue. In this work, we fabricated porous polyetherimide (PEI) scaffolds using a three-dimensional (3D) printing system, and the pore size was set as 800 μm. The morphology of 3D PEI scaffolds was characterized by the scanning electron microscope. To investigate the mechanical properties of the 3D PEI scaffold, the compressive mechanical test was performed via an electronic universal testing system. For the in vitro cell experiment, bone marrow stromal cells (BMSCs) were cultured on the surface of the 3D PEI scaffold and PEI slice, and cytotoxicity, cell adhesion, and cell proliferation were detected to verify their biocompatibility. Besides, the alkaline phosphatase staining and Alizarin Red staining were performed on the BMSCs of different samples to evaluate the osteogenic differentiation. Through these studies, we found that the 3D PEI scaffold showed an interconnected porous structure, which was consistent with the design. The elastic modulus of the 3D PEI scaffold (941.33 ± 65.26 MPa) falls in the range of modulus for the native cancellous bone. Moreover, the cell proliferation and morphology on the 3D PEI scaffold were better than those on the PEI slice, which revealed that the porous scaffold has good biocompatibility and that no toxic substances were produced during the progress of high-temperature 3D printing. The osteogenic differentiation level of the 3D PEI scaffold and PEI slice was equal and ordinary. All of these results suggest the 3D printed PEI scaffold would be a potential strategy for bone tissue engineering.

Improved cell adhesion of poly(amino acid) surface by cyclic phosphonate modification for bone tissue engineering

2018 ◽  
Vol 135 (21) ◽  
pp. 46226 ◽  
Author(s):  
Yi Xiong ◽  
Hong Li ◽  
Peng Wang ◽  
Pengzhen Liu ◽  
Yonggang Yan
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Amino Acid ◽  
Bone Tissue Engineering ◽  
Bone Tissue

scholarly journals Calcium Carbonate/Gelatin Methacrylate Microspheres for 3D Cell Culture in Bone Tissue Engineering

2020 ◽  
Vol 26 (8) ◽  
pp. 418-432
Author(s):  
Pengwei Xu ◽  
Fuliang Jiang ◽  
Hongbo Zhang ◽  
Ruixue Yin ◽  
Lian Cen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Calcium Carbonate ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
3D Cell Culture

Designing a novel nanocomposite for bone tissue engineering using electrospun conductive PBAT/polypyrrole as a scaffold to direct nanohydroxyapatite electrodeposition

RSC Advances ◽  
2016 ◽  
Vol 6 (39) ◽  
pp. 32615-32623 ◽  
Author(s):  
Juçara G. de Castro ◽  
Bruno V. M. Rodrigues ◽  
Ritchelli Ricci ◽  
Maíra M. Costa ◽  
André F. C. Ribeiro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Support Cell

Electrospinning is a well-recognized technique for producing nanostructured fibers with different functionalities, generating materials that are able to support cell adhesion and further proliferation.

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