Plasma Modification of PCL Porous Scaffolds Fabricated by Solvent-Casting/Particulate-Leaching for Tissue Engineering

2014 ◽  
Vol 11 (2) ◽  
pp. 184-195 ◽  
Author(s):  
Francesca Intranuovo ◽  
Roberto Gristina ◽  
Francesco Brun ◽  
Sara Mohammadi ◽  
Giacomo Ceccone ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Plasma Modification ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Particulate Leaching

scholarly journals Preparation and Characterization of Porous Scaffolds Based on Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

Life ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 935
Author(s):  
Asiyah Esmail ◽  
João R. Pereira ◽  
Chantal Sevrin ◽  
Christian Grandfils ◽  
Ugur Deneb Menda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Porous Scaffolds ◽  
High Porosity ◽  
Solvent Casting ◽  
Elongation At Break ◽  
Particulate Leaching ◽  
Copolymer Poly ◽  
Water Swelling ◽  
Emulsion Templating

Poly(hydroxyalkanoates) (PHAs) with different material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), and the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate, P(3HB-co-3HV), with a 3HV of 25 wt.%, were used for the preparation of porous biopolymeric scaffolds. Solvent casting with particulate leaching (SCPL) and emulsion templating were evaluated to process these biopolymers in porous scaffolds. SCPL scaffolds were highly hydrophilic (>170% swelling in water) but fragile, probably due to the increase of the polymer’s polydispersity index and its high porosity (>50%). In contrast, the emulsion templating technique resulted in scaffolds with a good compromise between porosity (27–49% porosity) and hydrophilicity (>30% water swelling) and without impairing their mechanical properties (3.18–3.35 MPa tensile strength and 0.07–0.11 MPa Young’s Modulus). These specifications are in the same range compared to other polymer-based scaffolds developed for tissue engineering. P(3HB-co-3HV) displayed the best overall properties, namely, lower crystallinity (11.3%) and higher flexibility (14.8% elongation at break. Our findings highlight the potency of our natural biopolyesters for the future development of novel porous scaffolds in tissue engineering, thanks also to their safety and biodegradability.

Fabrication and Properties of Porous Scaffolds of PLA-PEG Biocomposite for Bone Tissue Engineering

2014 ◽  
Vol 789 ◽  
pp. 130-135 ◽  
Author(s):  
Ning Wang ◽  
Yong Ju Zang ◽  
Gui Zhi Ren ◽  
Qi Lin Wu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Tetrazolium Salt ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Diphenyltetrazolium Bromide ◽  
Murine Fibroblast ◽  
Particulate Leaching

Porous scaffolds of polylactic acid-polyethylene glycol block copolymers (PLA-PEG) biocomposite were fabricated by solvent casting-particulate leaching method using sodium chloride as the porogen. With the aim of evaluating the influence of porosity on mechanical properties and biocompatibility, three specimens of scaffolds which have different porosity (around 50%, 60%, 70%) were fabricated. Murine fibroblast grew cells (L929) were seeded into PLA-PEG porous biocomposite scaffolds. The tetrazolium salt 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium Bromide (MTT), scanning electron microscopy and confocal microscopy were carried out to characterize cell proliferation and morphology. The composite scaffolds with the porosity of 50% possessed better mechanical properties. All scaffolds support attachment, spreading and proliferation of L929, and the biocompatibility of scaffolds could be improved by increasing the porosity. The fabricated PLA-PEG porous biocomposite scaffolds with good mechanical properties and biocompatibility might be used in bone tissue engineering.

scholarly journals Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yanbo Zhang ◽  
Ruiyan Li ◽  
Wenzheng Wu ◽  
Yun’an Qing ◽  
Xiongfeng Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Cell Adhesion ◽  
Phase Composition ◽  
Cell Morphology ◽  
Solvent Casting ◽  
Water Contact ◽  
Particulate Leaching ◽  
Cell Adhesion And Proliferation ◽  
Better Than

The purpose of this work was to investigate the porous polyetherimide scaffold (P-PEIs) as an alternative biopolymer for bone tissue engineering. The P-PEIs was fabricated via solvent casting and particulate leaching technique. The morphology, phase composition, roughness, hydrophilicity, and biocompatibility of P-PEIs were evaluated and compared with polyetherimide (PEI) and Ti6Al4V disks. P-PEIs showed a biomimetic porous structure with a modulus of 78.95 ± 2.30 MPa. The water contact angle of P-PEIs was 75.4 ± 3.39°, which suggested that P-PEIs had a wettability surface. Moreover, P-PEIs provides a feasible environment for cell adhesion and proliferation. The relative cell adhesion capability and the cell morphology on P-PEIs were better than PEI and Ti6Al4V samples. Furthermore, the MC3T3-E1 cells on P-PEIs showed faster proliferation rate than other groups. It was revealed that the P-PEIs could be a potential material for the application of bone regeneration.

Thermal Processing of Tissue Engineering Scaffolds

2010 ◽  
Vol 133 (3) ◽  
Author(s):  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Thermal Processing ◽  
Three Dimensional ◽  
Processing Parameters ◽  
High Pressure Processing ◽  
Porous Scaffolds ◽  
Tissue Engineering Scaffolds ◽  
Thermally Induced ◽  
Advantages And Disadvantages ◽  
Particulate Leaching

Tissue engineering requires complex three-dimensional scaffolds that mimic natural extracellular matrix function. A wide variety of techniques have been developed to create both fibrous and porous scaffolds out of polymers, ceramics, metals, and composite materials. Existing techniques include fiber bonding, electrospinning, emulsion freeze drying, solvent casting/particulate leaching, gas foaming/particulate leaching, high pressure processing, and thermally induced phase separation. Critical scaffold properties, including pore size, porosity, pore interconnectivity, and mechanical integrity, are determined by thermal processing parameters in many of these techniques. In this review, each tissue engineering scaffold preparation method is discussed, including recent advancements as well as advantages and disadvantages of the technique, with a particular emphasis placed on thermal parameters. Improvements on these existing techniques, as well as new thermal processing methods for tissue engineering scaffolds, will be needed to provide tissue engineers with finer control over tissue and organ development.

Porous polymeric scaffolds for bone regeneration

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Katarzyna Filipczak ◽  
Ireneusz Janik ◽  
Marek Kozicki ◽  
Piotr Ulanski ◽  
Janusz M. Rosiak ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Total Porosity ◽  
Polymer Chains ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Polymeric Scaffolds ◽  
Good Biocompatibility ◽  
Particulate Leaching ◽  
Highly Porous

AbstractSolvent casting/particulate leaching has been used to synthesize highly porous polymeric scaffolds of controlled pore size, based on poly(methyl methacrylate) (PMMA) and poly(ε-caprolactone) (PCL). Obtained structures have a total porosity of c. 60%, with good interconnections between the pores. Porous scaffolds prepared using the greatest size of NaCl particles have the best mechanical properties. Both PMMA- and PCL-based materials can be sterilized by ionizing radiation. In the case of PCL-based scaffolds, irradiation causes cross-linking of polymer chains, which leads to an improvement of the mechanical properties of the scaffold. The compressive elastic modulus for non-porous samples increases with irradiation dose from 1.5 MPa for 0 kGy to 1.9 MPa for 280 kGy. Preliminary in vitro studies indicate good biocompatibility of both materials.

New Method Used to Prepare Paraffin Spheres Based on Digitalization of Microfluids Technology

2011 ◽  
Vol 228-229 ◽  
pp. 1075-1079 ◽  
Author(s):  
Mei Yang ◽  
Li Ya Hou ◽  
Wei Yi Zhang
Keyword(s):  
Tissue Engineering ◽  
New Method ◽  
Solvent Casting ◽  
Drive Voltage ◽  
Particulate Leaching ◽  
Two Parameters

In tissue engineering, paraffin spheres are often used as porogen in solvent casting/particulate leaching technology to prepare scaffold. Shape, size of paraffin spheres is very important to connectivity of scaffold. Emulsification is commonly used to prepare paraffin spheres, in which the paraffin size can not be controlled well. In this paper, a new method based on digitalization of microfluids technology is used to produce paraffin spheres. In this method, relationship between drive voltage U and diameter of spheres, and relationship between diameter of nozzle d and diameter of spheres are studied. The two parameters have great effect on the shape, size of paraffin spheres. The shape, size of paraffin spheres can be adjusted by controlling the drive voltage U and diameter of nozzle d. The results show that the method based on digitalization of microfluids technology is simple, highly controllability.

Fabrication of poly(lactic acid) scaffolds by a modified solvent casting/porogen leaching method

e-Polymers ◽  
2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Xinghou Gong ◽  
Chak Yin Tang ◽  
Chi Tak Wong ◽  
William Weijia Lu ◽  
Yugang Zhang ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Pore Size ◽  
Three Dimensional ◽  
Poly Lactic Acid ◽  
Porous Scaffolds ◽  
Solvent Casting ◽  
Subsequent Removal ◽  
Modified Method ◽  
Good Accordance ◽  
Particulate Leaching

AbstractA modified solvent casting/particulate leaching (SC/PL) method was developed to fabricate thick three-dimensional poly(lactic acid) (PLA) scaffolds with controlled pore size and porosity, and much shorter processing time. The pasty mixture of PLA/CHCl3/NaCl was sealed in a Buchner funnel by a piston with a suction flask connected. A vacuum pump was used to facilitate the volatilization of solvent. The scaffolds were obtained by subsequent removal of NaCl in deionized water. The pore size within the scaffold was in good accordance with the particle size of NaCl (< 210, 210~310, 310~420 and 420~500 μm), all of which had more than 89 % of porosity, 1.95 ± 0.2 MPa of compressive modulus and non-cytotoxic to L929 cells. To demonstrate the applicability of the SC/VV/PL method, thick cylindrical and tubular porous scaffolds have been produced in this study. Compared with the traditional SC/PL without vacuum volatilization, the modified method, namely, solvent casting/vacuum volatilization/particulate leaching (SC/VV/PL) method, provide a more efficient way to fabricate thick scaffolds.

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