scholarly journals Helicteric Acid, Oleanic Acid, and Betulinic Acid, Three Triterpenes fromHelicteres angustifoliaL., Inhibit Proliferation and Induce Apoptosis in HT-29 Colorectal Cancer Cells via Suppressing NF-κB and STAT3 Signaling

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Dan Su ◽  
Yu-qiao Gao ◽  
Wei-bo Dai ◽  
Ying Hu ◽  
Yan-fen Wu ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Betulinic Acid ◽  
Biological Activities ◽  
Colorectal Tumor ◽  
Antitumor Effects ◽  
Chinese Medicinal Herb ◽  
Stat3 Signaling ◽  
Colorectal Cancer Cells ◽  
Ht 29

Colorectal cancer (CRC) is one of the most common malignancies and most frequent cause of cancer death worldwide. The activation of both NF-κB and STAT3 signaling and the crosstalk between them play an important role in colorectal tumor.Helicteres angustifoliaL. is a type of commonly used Chinese medicinal herb and possesses a wide variety of biological activities. In the present study, we investigate the effects of three triterpenes fromH. angustifolia(HT) such as helicteric acid (HA), oleanic acid (OA), and betulinic acid (BA), on inhibiting CRC progression. Our results showed that HT extracts could decrease proliferation and induce apoptosis in HT-29 colorectal cancer cells. Moreover, HT extracts could suppress LPS-triggered phosphorylation of IKK, IκB, and NF-κB, attenuate IL-6-induced phosphorylation of JAK2 and STAT3, and suppress the expression of c-Myc, cyclin-D1, and BCL-xL, the downstream gene targets of NF-κB and STAT3. Therefore, HT extracts showed potent therapeutic and antitumor effects on CRC via inhibiting NF-κB and STAT3 signaling.

scholarly journals α-Linolenic and γ-linolenic acids exercise differential antitumor effects on HT-29 human colorectal cancer cells

2020 ◽  
Vol 9 (4) ◽  
pp. 474-483 ◽  
Author(s):  
María José González-Fernández ◽  
Ignacio Ortea ◽  
José Luis Guil-Guerrero
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Linolenic Acid ◽  
Caspase 3 ◽  
Colorectal Cancer Cell Line ◽  
Human Colorectal Cancer ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells ◽  
Microscopy Images ◽  
Ht 29

Abstract α-Linolenic acid (ALA, 18:3n-3) and γ-gamma linolenic acid (GLA, 18:3n-6) are polyunsaturated fatty acids (PUFA) that improve the human health. The present study focused on testing the in vitro antitumor actions of pure ALA and GLA on the HT-29 human colorectal cancer cell line. Cell viability was checked by MTT ((3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) test, cell membrane damage by the lactate dehydrogenase assay, apoptosis was tested by both caspase-3 activity trial and transmission electron microscopy images, and protein composition was analyzed by quantitative proteomics analysis. MTT test revealed IC50 values of 230 and 255 μM for ALA and GLA, respectively, at 72 h. After 24 h of incubation, both ALA and GLA induced apoptosis on HT-29 colorectal cancer cells according to the caspase-3 assay and microscopy images. SWATH/MS analysis evidenced that ALA significantly affected the mitochondrial protein import pathway and the citric acid cycle pathway, while GLA did not significantly affect any particular pathway. In summary, both ALA and GLA showed concentration-dependent inhibitory effects on HT-29 cells viability and induced cell death by apoptosis. ALA significantly affected cellular pathways, while GLA does not have specific actions on either pathway. Both n-3 and n-6 C18 PUFA are bioactive food components useful in the colorectal cancer prevention.

scholarly journals Production of nanoliposomes with piperine from black pepper (Piper nigrum) and its improved growth inhibitory activity on colorectal cancer cells

2021 ◽  
Vol 18 (4) ◽  
pp. 671-678
Author(s):  
Le Nhat Minh ◽  
Tran Thi Minh Anh ◽  
Tran Van Loc ◽  
Phung Thi Kim Hue ◽  
Do Thi Thao
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Biological Activities ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Cytotoxic Activities ◽  
Growth Inhibitory ◽  
Colorectal Cancer Cells ◽  
Ht 29

Black pepper (Piper nigrum) is an autoicous and decorous vine cultivated in many local regions of Gia Lai. Black pepper is one of the most commonly consumed spices, and its pungency is due to the presence of alkaloids, such as piperine. This compound represents diverse biological activities, including anti-inflammatory, anticancer, antiviral, anti-larvicidal, pesticide, anti-alzheimer’s activities, etc. However, due to its poor solubility as well as its toxic effects at high use concentration, piperine is still in limit of pharmaceutical applications. In this study, we have used black pepper seed collected at Chu Se - Gia Lai to extract piperine. The compound extracted efficiency was approximately 18% with 96.7% of purity. Based on the obtained pure piperine, the hybrid nanopiperine-CD133 monoclonal antibody (mAb^CD133) complexes were fabricated with the nanoparticle size of about 170 nm, the polydispersity index (PDI) of 0.23 and the zeta potential of -9.4 mV. The nanocomplex was subjected for growth inhibitory activities against cancer colorectal cells (HT-29 cell line). The results showed that the nanopiperine-mAb^CD133 complex exhibited significant in vitro growth inhibition HT-29 colorectal cancer cells (46.56 ± 2.78%), while the viability of healthy cells remained unaffected (17.77 ± 0.82 %). The nanocomplex could also label 12.17% of HT-29 cells, which was rather higher than 3.83% from mAb^CD133 conjugated phycoerythrin (PE) as positive control. The fabricated nanopiperine-mAb^CD133 complex has proved the enhanced cytotoxic activities against colorectal cancerous cells as well as promising biopharmaceutical potency.

Effect of the polysaccharides derived from Dendrobium officinale stems on human HT-29 colorectal cancer cells and a zebrafish model

2021 ◽  
pp. 100995
Author(s):  
Shengchang Tao ◽  
Chunlei Huang ◽  
Zhihong Tan ◽  
Shuna Duan ◽  
Xiaofeng Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Dendrobium Officinale ◽  
Zebrafish Model ◽  
Colorectal Cancer Cells ◽  
Ht 29

Crataegus azarolusLeaves Induce Antiproliferative Activity, Cell Cycle Arrest, and Apoptosis in Human HT-29 and HCT-116 Colorectal Cancer Cells

2015 ◽  
Vol 117 (5) ◽  
pp. 1262-1272 ◽  
Author(s):  
Nadia Mustapha ◽  
Aline Pinon ◽  
Youness Limami ◽  
Alain Simon ◽  
Kamel Ghedira ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Antiproliferative Activity ◽  
Cycle Arrest ◽  
Colorectal Cancer Cells ◽  
Hct 116 ◽  
Ht 29

scholarly journals Autophagy inhibition by chloroquine sensitizes HT-29 colorectal cancer cells to concurrent chemoradiation

2014 ◽  
Vol 6 (3) ◽  
pp. 74 ◽  
Author(s):  
Caitlin A Schonewolf ◽  
Monal Mehta ◽  
Devora Schiff ◽  
Hao Wu ◽  
Bruce G Haffty ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Concurrent Chemoradiation ◽  
Colorectal Cancer Cells ◽  
Autophagy Inhibition ◽  
Ht 29

Evaluation of RAC1 Gene Expression Under Exposure Of Plasma Activated Verbascoside in HT-29 Colorectal Cancer Cells

2018 ◽  
Vol 9 ◽  
pp. 36-37
Author(s):  
Kobra Hajizadeh ◽  
Bahram Behzad ◽  
Danial Seifi ◽  
Hassan Mehdian ◽  
Mohammad Nabiouni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Colorectal Cancer ◽  
Cancer Cells ◽  
Colorectal Cancer Cells ◽  
Ht 29

scholarly journals Combination of oncolytic adenovirus and luteolin exerts synergistic antitumor effects in colorectal cancer cells and a mouse model

2017 ◽  
Vol 16 (6) ◽  
pp. 9375-9382 ◽  
Author(s):  
Boduan Xiao ◽  
Yun Qin ◽  
Chang Ying ◽  
Buyun Ma ◽  
Binrong Wang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Cancer Cells ◽  
Oncolytic Adenovirus ◽  
Antitumor Effects ◽  
Colorectal Cancer Cells

scholarly journals miR-214 sensitizes human colorectal cancer cells to doxorubicin by p53 targeting

2020 ◽  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
P53 Expression ◽  
Colorectal Cancer Cells ◽  
Scratch Wound ◽  
And Migration ◽  
Induced Apoptosis ◽  
Ht 29 ◽  
Human Colorectal Cancer Cells

Objectives: This study aimed to investigate the potential function of miR-214 in the apoptosis induction by targeting p53 in human colorectal cancer cells (CRC) in combination with doxorubicin (DOX). Methods: miR-214 mimics were transfected to HT-29 CRC cells. Following that, the transfected cells were treated with DOX. Cell viability, apoptosis, and migration were evaluated by MTT, flow cytometry, and scratch-wound motility assays, respectively. Furthermore, the expression level of miR-214 and p53 was evaluated by qRT-PCR. Results: miR-214 transfection significantly inhibited the cell proliferation rate (P<0.05), induced apoptosis (P<0.05), and harnessed migration (P<0.05) in the HT-29 cells after 48 h. Furthermore, more effectiveness was observed in combination with DOX. Additionally, miR-214 transfection led to a reduction in p53 expression offering that it might be a potential target for miR-214. Conclusion: In conclusion, miR-214 sensitizes HT-29 cells to doxorubicin by targeting p53 indicating the significant role of this miRNA in colorectal cancer chemotherapy.

scholarly journals Assessment of TRPV4 Channel and Its Role in Colorectal Cancer Cells

2019 ◽  
Vol 12 (2) ◽  
pp. 629-638
Author(s):  
N. N. Bahari ◽  
S. Y. N. Jamaludin ◽  
A. H. Jahidin ◽  
M. N. Zahary ◽  
A. B. Mohd Hilmi
Keyword(s):  
Colorectal Cancer ◽  
Cell Cycle ◽  
Cell Death ◽  
Cancer Cells ◽  
Human Colorectal Cancer ◽  
Expression Levels ◽  
Pharmacological Modulation ◽  
Colorectal Cancer Cells ◽  
Ht 29

The transient receptor potential vanilloid member 4 (TRPV4) is a non-selective calcium (Ca2+)-permeable channel which is widely expressed in different types of tissues including the lungs, liver, kidneys and salivary gland. TRPV4 has been shown to serve as a cellular sensor where it is involved in processes such as osmoregulation, cell volume regulation and thermoregulation. Emerging evidence suggests that TRPV4 also plays important roles in several aspects of cancer progression. Despite the reported roles of TRPV4 in several forms of cancers, the role of TRPV4 in human colorectal cancer remains largely unexplored. In the present study, we sought to establish the potential role of TRPV4 in colorectal cancer by assessing TRPV4 expression levels and investigating whether TRPV4 pharmacological modulation may alter cell proliferation, cell cycle and cell death in colorectal cancer cells. Quantitative real-time PCR analysis revealed that TRPV4 mRNA levels were significantly lower in HT-29 cells than normal colon CCD-18Co cells. However, TRPV4 mRNA was absent in HCT-116 cells. Pharmacological activation of TRPV4 with GSK1016790A significantly enhanced the proliferation of HT-29 cells while TRPV4 inhibition using RN 1734 decreased their proliferation. Increased proliferation in GSK1016790A-treated HT-29 cells was attenuated by co-treatment with RN 1734. Pharmacological modulation of TRPV4 had no effect on the cell cycle progression but promoted cell death in HT-29 cells. Taken together, these findings suggest differential TRPV4 expression levels in human colorectal cancer cells and that pharmacological modulation of TRPV4 produces distinct effects on the proliferation and induces cell death in HT-29 cells.

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