scholarly journals Anticonvulsant and Neuroprotective Activities ofPhragmanthera austroarabicaExtract in Pentylenetetrazole-Kindled Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Hibah M. Aldawsari ◽  
Basma G. Eid ◽  
Thikrayat Neamatallah ◽  
Sawsan A. Zaitone ◽  
Jihan M. Badr
Keyword(s):  
Plant Extract ◽  
Hippocampal Neurons ◽  
Seizure Activity ◽  
Chemical Constituents ◽  
Neuroprotective Effect ◽  
Intraperitoneal Administration ◽  
Neuroprotective Activity ◽  
Total Phenolic ◽  
Control Group ◽  
Phenolic Contents

Anticonvulsant and neuroprotective activity ofPhragmanthera austroarabicaextract were tested in pentylenetetrazole-kindled mice. All the chemical constituents of the plant extract were identified. Additionally, the extract was standardized and proved to contain total phenolic contents equal to379.92±1.32 mg gallic acid equivalents/g dry plant extract. Induction of kindling was achieved by repeated intraperitoneal administration of pentylenetetrazole (35 mg/kg) twice weekly. Male albino mice were givenP.austroarabicaextract (200, 400, or 800 mg/kg). The two higher doses (400 or 800 mg/kg) of the extract significantly caused notable reduction in seizure activity and hippocampal malondialdehyde level compared to pentylenetetrazole control group. The highest dose enhanced cortical GSH level and showed intact DNA in the laddering assay. Upon studying the neuroprotective effect, mice treated with the higher dose of the extract demonstrated an improvement in the percent of surviving neurons in the cortex and hippocampus. We concluded thatP. austroarabicaextract ameliorated seizure activity and protected cortical and hippocampal neurons against pentylenetetrazole-induced kindling in mice.

Neuroprotective Activity of the Methanolic Extract of Indigofera aspalathoides against Scopalamine induced Alzheimer's Disease in Experimental Rats

2021 ◽  
pp. 5163-5168
Author(s):  
Rajaram C. ◽  
S. Nelson Kumar ◽  
S. S. Sheeba Tabassum ◽  
Manohar R. ◽  
Sumanjali C.
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Traditional Medicine ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Neuroprotective Effect ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Histological Structure ◽  
Anticancer Activities

The plant Indigofera aspalathoides is a traditional medicine with tremendous therapeutic potential which finds it use in treatment of various ailments such as antibacterial, antioxidant, anti-inflammatory, antidiabetic, and anticancer activities. There are no reports that related to the use of this plant in treating patients with Alzheimer’s disease (AD). Hence present study was aimed to scientifically evaluate the neuroprotective effect of the methanolic extract of Indigofera aspalathoides against scopalamine induced Alzheimer’s disease in experimental rats using behavioral tests like elevated plus maze, Y-maze, and rota-rod tests. In addition to this, biochemical evaluation for acetylcholinesterase activity and histopathological evaluation of brain were done. The results suggests that methanolic extract Indigofera aspalathoides (200mg/kg B.wt and 400mg/kg B.wt) used in this study shows significant improvement of various behavioral parameters like locomotion, anxiety, memory, motor integrity and coordination etc when compared to control group. MEIA inhibited brain AChE enzyme, thereby elevating Ach concentration in brain homogenate and ultimately improved memory of rats. Further, more or less normal histological structure of the hippocampus and all amyloid plaques and neurofibrillary tangles that are formed under the influence of scopolamine disappeared in the rats pretreated with MEIA (200mg/kg B.wt and 400mg/kg B.wt). It can be concluded that our results strongly support the anti-Alzheimer’s potential of the methanolic extract of the plant I.aspalathoides and its use in traditional medicine.

scholarly journals Antioxidant and chemical properties of Inula helenium root extracts

2013 ◽  
Vol 11 (10) ◽  
pp. 1699-1709 ◽  
Author(s):  
Iuliana Spiridon ◽  
Constantin Nechita ◽  
Marius Niculaua ◽  
Mihaela Silion ◽  
Alice Armatu ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Chemical Properties ◽  
Total Phenolic ◽  
Egg Albumin ◽  
Root Extracts ◽  
Phenolic Contents ◽  
Rat Paw Edema ◽  
Inula Helenium ◽  
Anti Inflammatory ◽  
Rat Paw

AbstractThe objective of the paper was to investigate the chemical composition of Inula helenium roots extracts and to evaluate the antioxidant potential conferred by the chemical constituents. GC/MS and HPLC/MS techniques were used to characterize two extracts separated from Inula helenium roots by extraction with chloroform and ethyl acetate, respectively. Volatile compounds have been identified by GC from their mass spectra and retention time values, while HPLC identification of phenolic compounds was realized by comparing their retention times, UV and MS spectra with those of standards or literature data. Measurements of antioxidant activity of Inula helenium root extracts showed a variation between them, which can be correlated with the flavonoid and total phenolic contents. Both Inula helenium root extracts contain phenolic acids (caffeic, chlorogenic, dicaffeoyl quinic, hydroxibenzoic), terpenes (alantolactone) and different flavonoids (epicatechin, catechin gallate, ferulic acid-4-O-glucoside, dihydroquercetin pentosyl rutinoside, kaempherol-7-O-dipentoside, quercetin-3-O-β-glucopyranoside). In addition, the study provides preliminary data on the anti-inflammatory activity of Inula helenium root extracts, this being evaluated using the fresh egg albumin as phlogistic agent, and aspirin as reference compound. Root extracts of I. helenium did not exert any significant anti-inflammatory effect on egg albumin-induced rat paw edema.

scholarly journals NEUROPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF AMARANTHUS TRICOLOR LEAVES ON EXPERIMENTAL ANIMALS

2020 ◽  
pp. 181-186
Author(s):  
VIDHAN CHAND BALA ◽  
MOHD ABID
Keyword(s):  
Blood Cell ◽  
Neuroprotective Effect ◽  
Research Work ◽  
Antimicrobial Activities ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Amaranthus Tricolor ◽  
Hydroalcoholic Extract ◽  
Central Nervous System Activity

Objective: The research work deals with the screening of hydroalcoholic extract of Amaranthus tricolor (HAEATL) leaves for central nervous system activity (anti-stress, nootropic, and anti-cataleptic activity). Methods: The screening of scopolamine and different model-induced neurodisorder rats were treated with 200 and 400 mg/kg body weight dose of hydroalcoholic extract of A. tricolor lives in 7 days’ experimental schedule. It has been reported that the antioxidant, hepatoprotective, hematological, and antimicrobial activities. Results: The result of the study reflected that HAEATL (200 and 400 mg/kg) was effective in all methods and showed anti-stress, nootropic, and anti-cataleptic activity in a dose-dependent manner. The results are represented that the HAEATL produce the significant decreased the swimming time, increased the anoxia time, decreased level of biochemical parameters such as glucose and cholesterol except blood urea nitrogen, decreased level of white blood cell and red blood cell, and more significantly decreased the catalepsies score on 7th day compared to the control group. Conclusion: The above valuable animal study, we concluded that the HAEATL showing significantly affect compeer to the disease control group on neuroprotective activity.

scholarly journals ADDITIVE NEUROPROTECTIVE EFFECT OF 3-HYDROXYPYRIDINE DERIVATIVES AND HUMAN ERYTHROPOETIN ANALOGUE ON A HEMORRHAGIC STROKE MODEL IN RATS

2020 ◽  
Vol 8 (3) ◽  
pp. 169-180
Author(s):  
P. D. Kolesnichenko ◽  
O. V. Scheblykina ◽  
N. I. Nesterova ◽  
D. V. Scheblykin ◽  
A. V. Nesterov ◽  
...  
Keyword(s):  
Survival Rate ◽  
Hemorrhagic Stroke ◽  
Neuroprotective Effect ◽  
Cerebrovascular Disorders ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Stroke Model ◽  
Combined Use ◽  
Perifocal Edema ◽  
The Brain

The correction of free radical oxidation processes is one of the most promising strategies of neuroprotection in acute cerebrovascular disorders.The aim of the study is an experimental study of the neuroprotective effects of 3-hydroxypyridine and erythropoietin derivatives, as well as their combined use.Materials and methods. The study was performed on 109 male Wistar rats. The neuroprotective effect of the substances was studied on a hemorrhagic stroke model. The study drugs were administered to the animals intraperitoneally. Carbamylated darbepoetin was administered three times in advance at the dose of 100 µg/kg within intervals of 3 days, the last injection took place 1 hour before the operation (the total dose was 300 mg/kg). Etoxidol was administered once 1 hour before the surgery at the dose of 50 mg/kg. The survival rate, behavioral features and the state of the animals on the 1st, 3rd, 7th and 14th days were recorded, and the morphological assessment of the brain was carried out.Results. The investigated substances had a positive effect on both the survival rate of the animals during the first day and on the 14th day. The best survival rates on the 14th day were recorded in the group of a combined use of ethoxydol and carbamylated darbepoetin (75%). Thus, in this group of rats, a faster recovery of neurological disorders was already distinguished from the first day on. By the 7th day, more than 50% of the rats receiving the combination of the studied drugs, had had a slight neurological deficit (up to 3 points on the McGrow scale); by the 14th day there had been only minor changes in the neurological status in the rats of this group. A pronounced neuroprotective effect of the combination of 3-hydroxypyridine and erythropoietin derivatives has been confirmed by a histological examination of brain slices – a more rapid decrease in the size of perifocal edema and microcirculation disorders, less damage to neurons and glial elements, and faster processes of resorption and organization of hemorrhage. A macroscopic examination of the brain sections stained with triphenyltetrazolium chloride of the dying rats, showed that perifocal necrosis had been the main cause of high mortality in the control group after the 3rd day.Conclusion. As a result of the experiment, the nephroprotective effect of the studied derivatives of 3-hydroxypyridine and erythropoietin has been proved. Moreover, the combination of these drugs has shown a greater neuroprotective activity than their isolated use. The additive effect of these drugs was due to their action mechanism resulting from the synergism of various structures and components of the cells.

scholarly journals Neuroprotective Activity of Water Soluble Extract fromChorispora bungeanaagainst Focal Cerebral Ischemic/Reperfusion Injury in Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Ya-Xuan Sun ◽  
Ting Liu ◽  
Xue-Ling Dai ◽  
Ya-Bo Li ◽  
Yu-Yao Li ◽  
...  
Keyword(s):  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Water Extract ◽  
Water Soluble ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Chorispora Bungeana ◽  
Model Control ◽  
Water Soluble Extract ◽  
Cerebral Ischemic

The purpose of the present study was to clarify whether the water extract ofChorispora bungeanawas an antioxidant agent against cerebral ischemia/reperfusion (I/R). Our results showed that water extract ofChorispora bungeanatreatment significantly reduced neurological deficit scores, infarct size, MDA and carbonyl contents, and GSH/GSSG ratio compared with the model control group. After being treated byChorispora bungeana, SOD, CAT, and GSH-Px activities remarkably increased.Chorispora bungeanatreatment also improved 8-OHdG expression and cell apoptosis. Our findings indicated that the water extract ofChorispora bungeanapossesses neuroprotective effect which is most likely achieved by antioxidant and antiapoptotic activities.

scholarly journals Phenolic Compounds Isolated and Identified from Amla (Phyllanthus emblica) Juice Powder and their Antioxidant and Neuroprotective Activities

2018 ◽  
Vol 13 (10) ◽  
pp. 1934578X1801301 ◽  
Author(s):  
Kenneth Rose ◽  
Chunpeng Wan ◽  
Amber Thomas ◽  
Navindra P. Seeram ◽  
Hang Ma
Keyword(s):  
Gallic Acid ◽  
Ellagic Acid ◽  
Chemical Constituents ◽  
Butylated Hydroxytoluene ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Phyllanthus Emblica ◽  
Neuroprotective Effects ◽  
Potential Health

The edible fruit of Phyllanthus emblica (known as amla and Indian gooseberry) is widely used in Eastern traditional medicinal systems for a variety of ailments. Our group has previously reported that an amla juice powder shows neuroprotective effects in several in vitro and in vivo assay models but its chemical constituents and their neuroprotective activity remain unknown. Therefore, we conducted a phytochemical investigation of amla juice powder and evaluated the antioxidant and neuroprotective effects of the isolates. Ten phenolics (1–10), including gallic acid (1), five gallic acid derivatives (2–6), ellagic acid (7), and three ellagic acid derivatives (8–10), were isolated and identified with compounds 8–10 being reported from amla for the first time. All of the isolates showed antioxidant effects in the DPPH assay with IC50 values ranging from 6-158.9 μM superior to the synthetic commercial antioxidant, butylated hydroxytoluene (IC50 = 371.4 μM). In addition, compound 8 reduced β-amyloid-induced neurotoxicity in a transgenic Caenorhabditis elegans model by increasing their survival rate by 28.3% compared to the control group. This study adds to the growing body of evidence supporting the potential health benefits of amla and supports the functional food and nutraceutical applications of amla juice powder.

scholarly journals The Protective Effect of Radix Polygoni Multiflori on Diabetic Encephalopathy via Regulating Myosin Light Chain Kinase Expression

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Yu He ◽  
Feng Wang ◽  
Shiqiang Chen ◽  
Mi Liu ◽  
Wei Pan ◽  
...  
Keyword(s):  
Hippocampal Neurons ◽  
Neuroprotective Effect ◽  
Diabetic Rats ◽  
Control Group ◽  
Dependent Manner ◽  
Diabetic Encephalopathy ◽  
Diabetes Group ◽  
Group A ◽  
Group B ◽  
Kinase Expression

Currently there has been no effective treatment of diabetic encephalopathy. Radix Polygoni Multiflori, a famous traditional Chinese medicine, is widely used in antiaging treatment, especially in prevention and treatment of Alzheimer’s diseases. In this study we tried to explore the effect of Radix Polygoni Multiflori on cognitive function among diabetic rats with demonstrated cognitive impairment. SD rats were divided into group A (control group), group B (diabetes), group C (treated with Radix Polygoni Multiflori at the dose of 2 g/kg/d), and group D (treated with same drug at the dose of 1 g/kg/d). The results showed that 8 weeks of Radix Polygoni Multiflori treatment could improve the cognitive dysfunction of diabetic rats (P<0.01), recover the ultrastructure of hippocampal neurons, and increase the number of synapses in a dose-dependent manner. Further experiment also suggested that the neuroprotective effect of Radix Polygoni Multiflori was partly achieved by downregulating MLCK expression in hippocampus via ERK signaling.

scholarly journals NEUROPROTECTIVE EFFECTS OF POMEGRANATE JUICE ON LEAD INDUCED NEUROTOXICITY IN MICE

2017 ◽  
Vol 9 (2) ◽  
pp. 207
Author(s):  
Leila Gadouche ◽  
Noureddine Djebli ◽  
Khayra Zerrouki
Keyword(s):  
Drinking Water ◽  
Cerebral Cortex ◽  
Lead Acetate ◽  
Neuronal Degeneration ◽  
Neuroprotective Effect ◽  
Pomegranate Juice ◽  
Total Phenolic ◽  
Control Group ◽  
Histological Structure ◽  
Neuroprotective Effects

<p><strong>Objective: </strong>This study evaluates the potential neuroprotective of the pomegranate juice against chronic intoxication with lead acetate for 3<strong> </strong>months.</p><p><strong>Methods: </strong>Twenty-one female Swiss mice divided into 3 groups were employed in the present investigation. Control group: received drinking water for 90 days, neurotoxic group were exposed to 1000 ppm of lead acetate in the drinking water for 12 weeks, and neurotoxic treated group represents the mice received treatment with juice pomegranate diluted with distilled water (v/v) orally for 4 h / day followed by lead acetate at a dose of 1000 ppm orally for 20 h / day for 90 days. After cessation of treatment, neurobehavioral studies using the open field test, black and white test box and swimming test were made. In the next phase, brain injury was assessed histologically with hematoxylin-eosin staining.</p><p><strong>Results:</strong> Chronic exposure to lead led to significant increase in the level of anxiety, depression and the locomotor activity (P &lt; 0.05). It was confirmed by histopathological alterations in many areas of the cerebral cortex and hippocampus including neuronal degeneration and decrease cell density. Treatment with the juice significantly improve the level of depression, locomotor function (P &lt; 005) and anxiety (P &gt; 0.05) in mice exposed to lead as well as restored the histological structure in cerebral cortex and hippocampus of mice. The total phenolic and flavonoids content in juice of pomegranate was found to be 3809. 8±29.404 mg GAE/l; 2109. 57±18.936 mg QE /l of juice.</p><p><strong>Conclusion: </strong>This finding suggests that phenolic compounds found in pomegranate juice provide a neuroprotective effect on behavioural impairments and histopathological change induced by lead.</p>

scholarly journals Moringa oleifera Supplementation Improves Antioxidant Status and Biochemical Indices by Attenuating Early Pregnancy Stress in Beetal Goats

2021 ◽  
Vol 8 ◽  
Author(s):  
Ali Afzal ◽  
Tarique Hussain ◽  
Amjad Hameed
Keyword(s):  
Early Pregnancy ◽  
Antioxidant Status ◽  
Moringa Oleifera ◽  
Basal Diet ◽  
Total Phenolic ◽  
Control Group ◽  
Phenolic Contents ◽  
Beneficial Effects ◽  
Blood Biochemical ◽  
Biochemical Indices

This study investigated the effects of supplementing different levels of Moringa oleifera leaf powder (MOLP) on antioxidant status and blood biochemical indices during early gestation in Beetal goats. A total of 30 goats were randomly divided into three groups (n = 10) comprising control (basal diet without MOLP), the 1.6% MOLP supplemented group (basal diet + 1.6% MOLP), and the 3.2% MOLP supplemented group (basal diet + 3.2% MOLP). The experiment started 5 days before estrus synchronization and lasted till day 60 of gestation. The MOLP significantly increased plasma flavonoids in 1.6% as well as 3.2% supplemented group on days 40 and 60 of pregnancy, while total phenolic contents were observed to be higher in the 3.2% MOLP supplemented group throughout the experiment in comparison with the control group. The supplementation improved plasma total antioxidant capacity (TAC) by decreasing malondialdehyde (MDA) and total oxidant status (TOS) in contrast to the control group. The activities of superoxide dismutase (SOD) and peroxidase (POD) were enhanced in both supplemented groups, whereas catalase (CAT) activity was noticed to be significantly high in the 3.2% MOLP supplemented group. The protein contents were significantly elevated with 1.6 and 3.2% supplementation levels from day 40 to day 60 of the experiment. Plasma sugar level, carotenoids, progesterone profile, and hydrolytic (protease and amylase) enzymes activities were improved only when supplemented with 3.2% MOLP. The findings suggest that supplementing with 3.2% MOLP provides beneficial effects on early pregnancy stress in Beetal goats.

scholarly journals Pekmez ve Erik Kullanılarak Üretilen Kefirlerin Bazı Kalite Kriterlerinin Belirlenmesi

2014 ◽  
Vol 2 (2) ◽  
pp. 86 ◽  
Author(s):  
Tuğba Kök Taş ◽  
Erkut İlay ◽  
Ayla Öker
Keyword(s):  
Antioxidant Activity ◽  
Dry Matter ◽  
Storage Period ◽  
Titratable Acidity ◽  
Total Phenolic ◽  
Control Group ◽  
Phenolic Contents ◽  
Colour Parameter ◽  
Total Antioxidant ◽  
Lactobacillus Spp

The aim of this study is to determine the quality characteristics of kefir by developing different flavours in order to increase its consumption. Plum (10%) and molasses (7.5%) which are rich in phenolic contents are used to produce kefir in different flavours. Chemical analysis (pH, titratable acidity, dry matter and colour), microbial analysis (total microorganism, Lactobacillus spp, Lactococcus spp. and yeast counts), total antioxidant activity (by ORAC method), total amount of phenolic contents and sensorial properties of all kefir samples were carried out at the 1st, 7th and 14th days of storage. Consequently, the results of pH, titratable acidity and dry matter in the kefir samples were determined to change between the values 4.11-4.39; 0.22-0.30 and 11.9-17.02% respectively, during the storage. In the colour analysis, the redness colour parameter of the kefir sample produced using molasses was determined to be higher compared to the other kefir samples. At the first day of storage, the total antioxidant contents of the control group kefir, plum- added kefir and molasses-added kefir samples were determined as 13.30; 16.80 and 17.35 µmol ml-1; and the total phenolic contents as 945.70; 2535.8 and 2357.6 mg ml-1, respectively. Content of total microorganism in kefir samples varied between 8.91-9.80 log kob ml-1during the storage period. Lactobacillus spp. and Lactococcus spp. contents of kefir produced using molasses were determined to have the highest values with 9.11 and 9.91 log kob ml-1, respectively, at the first day. Yeast content of the plum kefir and the molasses kefir samples including sugar was detected to be higher than that of the controlled kefir sample. According to the results of the sensory analysis, the kefir produced with plum was approved the most by the panelists. As a result of this study, two alternative products with quite high total phenolic content and antioxidant activity were developed for consumers.

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