scholarly journals Neuroprotective Activity of Water Soluble Extract fromChorispora bungeanaagainst Focal Cerebral Ischemic/Reperfusion Injury in Mice

2014 ◽  
Vol 2014 ◽  
pp. 1-9
Author(s):  
Ya-Xuan Sun ◽  
Ting Liu ◽  
Xue-Ling Dai ◽  
Ya-Bo Li ◽  
Yu-Yao Li ◽  
...  
Keyword(s):  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Water Extract ◽  
Water Soluble ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Chorispora Bungeana ◽  
Model Control ◽  
Water Soluble Extract ◽  
Cerebral Ischemic

The purpose of the present study was to clarify whether the water extract ofChorispora bungeanawas an antioxidant agent against cerebral ischemia/reperfusion (I/R). Our results showed that water extract ofChorispora bungeanatreatment significantly reduced neurological deficit scores, infarct size, MDA and carbonyl contents, and GSH/GSSG ratio compared with the model control group. After being treated byChorispora bungeana, SOD, CAT, and GSH-Px activities remarkably increased.Chorispora bungeanatreatment also improved 8-OHdG expression and cell apoptosis. Our findings indicated that the water extract ofChorispora bungeanapossesses neuroprotective effect which is most likely achieved by antioxidant and antiapoptotic activities.

Neuroprotective Activity of the Methanolic Extract of Indigofera aspalathoides against Scopalamine induced Alzheimer's Disease in Experimental Rats

2021 ◽  
pp. 5163-5168
Author(s):  
Rajaram C. ◽  
S. Nelson Kumar ◽  
S. S. Sheeba Tabassum ◽  
Manohar R. ◽  
Sumanjali C.
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Traditional Medicine ◽  
Methanolic Extract ◽  
Therapeutic Potential ◽  
Neuroprotective Effect ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Histological Structure ◽  
Anticancer Activities

The plant Indigofera aspalathoides is a traditional medicine with tremendous therapeutic potential which finds it use in treatment of various ailments such as antibacterial, antioxidant, anti-inflammatory, antidiabetic, and anticancer activities. There are no reports that related to the use of this plant in treating patients with Alzheimer’s disease (AD). Hence present study was aimed to scientifically evaluate the neuroprotective effect of the methanolic extract of Indigofera aspalathoides against scopalamine induced Alzheimer’s disease in experimental rats using behavioral tests like elevated plus maze, Y-maze, and rota-rod tests. In addition to this, biochemical evaluation for acetylcholinesterase activity and histopathological evaluation of brain were done. The results suggests that methanolic extract Indigofera aspalathoides (200mg/kg B.wt and 400mg/kg B.wt) used in this study shows significant improvement of various behavioral parameters like locomotion, anxiety, memory, motor integrity and coordination etc when compared to control group. MEIA inhibited brain AChE enzyme, thereby elevating Ach concentration in brain homogenate and ultimately improved memory of rats. Further, more or less normal histological structure of the hippocampus and all amyloid plaques and neurofibrillary tangles that are formed under the influence of scopolamine disappeared in the rats pretreated with MEIA (200mg/kg B.wt and 400mg/kg B.wt). It can be concluded that our results strongly support the anti-Alzheimer’s potential of the methanolic extract of the plant I.aspalathoides and its use in traditional medicine.

scholarly journals Influence of dibornol-HES on electrophysiological parameters in the period of restoration of blood flow in rabbit myocardium

2018 ◽  
Vol 17 (4) ◽  
pp. 6-15
Author(s):  
M. A. Vaykshnorayte ◽  
V. A. Vityazev ◽  
N. A. Vahnina ◽  
V. D. Shadrina ◽  
M. A. Torlopov ◽  
...  
Keyword(s):  
Blood Flow ◽  
Coronary Occlusion ◽  
Ischemia Reperfusion ◽  
Myocardial Damage ◽  
Water Soluble ◽  
Control Group ◽  
Acute Ischemia ◽  
30Th Minute ◽  
Dispersion Of Repolarization ◽  
Experimental Group

Objective. Dibornol-HES, a water-soluble drug based on the derivative of 2,6-diisobornyl-4-methylphenol Dibornol conjugated with hydroxyethyl starch, can reduce the occurrence and severity of arrhythmias by preventive intravenous administration, but it is unknown whether the drug could reduce the myocardial arrhythmogenicity once ischemia has developed at the developed ischemia.Materials and methods. In the model of acute ischemia / reperfusion of the rabbit heart, the effect of Dibornol-HEC (80 mg/kg body weight of the animal) on the electrophysiological indices characterizing myocardial arrhythmogenicity (global and border dispersion of repolarization) was studied during the restoration of blood flow. In the model of acute ischemia / reperfusion with 64 unipolar epicardial leads, the activation-recovery intervals were measured and global and border dispersion of repolarization in the native rabbits (control group, n = 9) and in the rabbits treated by Dibornol-HES (on the 25th minute of occlusion, the experimental group, n = 6).Results. The introduction of Dibornol-HES did not lead to a change in the electrocardiographic parameters of rabbits. By the 30th minute of the coronary occlusion on the ECG in the animals of the control and the experimental groups, the intervals RR, QT, QTc were shortened (p < 0.05). In the animals of both groups by the 30th minute of coronary occlusion, the global dispersion of repolarization increased (p < 0.05), the boundary dispersion of repolarization also increased (p < 0.05), due to the decrease in the duration of the activation-recovery intervals in the ischemic zone (p < 0.05). During the 30-minute reperfusion the magnitude of the global dispersion of repolarization did not change in animals of the both groups, and the magnitude of the border dispersion of repolarization in the control rabbits decreased (p < 0.05), while in the rabbits treated by Dibornol-HES the border dispersion of repolarization did not changed.Conclusion. In rabbits of the experimental group, the values of the global and border dispersions of repolarization did not differ from those of the animals in the control group. Therefore, the administration to Dibornol-HES just prior to reperfusion does not lead to the decrease in the dispersion of repolarization increased as a result of acute ischemic myocardial damage.

scholarly journals Anticonvulsant and Neuroprotective Activities ofPhragmanthera austroarabicaExtract in Pentylenetetrazole-Kindled Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Hibah M. Aldawsari ◽  
Basma G. Eid ◽  
Thikrayat Neamatallah ◽  
Sawsan A. Zaitone ◽  
Jihan M. Badr
Keyword(s):  
Plant Extract ◽  
Hippocampal Neurons ◽  
Seizure Activity ◽  
Chemical Constituents ◽  
Neuroprotective Effect ◽  
Intraperitoneal Administration ◽  
Neuroprotective Activity ◽  
Total Phenolic ◽  
Control Group ◽  
Phenolic Contents

Anticonvulsant and neuroprotective activity ofPhragmanthera austroarabicaextract were tested in pentylenetetrazole-kindled mice. All the chemical constituents of the plant extract were identified. Additionally, the extract was standardized and proved to contain total phenolic contents equal to379.92±1.32 mg gallic acid equivalents/g dry plant extract. Induction of kindling was achieved by repeated intraperitoneal administration of pentylenetetrazole (35 mg/kg) twice weekly. Male albino mice were givenP.austroarabicaextract (200, 400, or 800 mg/kg). The two higher doses (400 or 800 mg/kg) of the extract significantly caused notable reduction in seizure activity and hippocampal malondialdehyde level compared to pentylenetetrazole control group. The highest dose enhanced cortical GSH level and showed intact DNA in the laddering assay. Upon studying the neuroprotective effect, mice treated with the higher dose of the extract demonstrated an improvement in the percent of surviving neurons in the cortex and hippocampus. We concluded thatP. austroarabicaextract ameliorated seizure activity and protected cortical and hippocampal neurons against pentylenetetrazole-induced kindling in mice.

scholarly journals NEUROPROTECTIVE EFFECT OF HYDROALCOHOLIC EXTRACT OF AMARANTHUS TRICOLOR LEAVES ON EXPERIMENTAL ANIMALS

2020 ◽  
pp. 181-186
Author(s):  
VIDHAN CHAND BALA ◽  
MOHD ABID
Keyword(s):  
Blood Cell ◽  
Neuroprotective Effect ◽  
Research Work ◽  
Antimicrobial Activities ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Dependent Manner ◽  
Amaranthus Tricolor ◽  
Hydroalcoholic Extract ◽  
Central Nervous System Activity

Objective: The research work deals with the screening of hydroalcoholic extract of Amaranthus tricolor (HAEATL) leaves for central nervous system activity (anti-stress, nootropic, and anti-cataleptic activity). Methods: The screening of scopolamine and different model-induced neurodisorder rats were treated with 200 and 400 mg/kg body weight dose of hydroalcoholic extract of A. tricolor lives in 7 days’ experimental schedule. It has been reported that the antioxidant, hepatoprotective, hematological, and antimicrobial activities. Results: The result of the study reflected that HAEATL (200 and 400 mg/kg) was effective in all methods and showed anti-stress, nootropic, and anti-cataleptic activity in a dose-dependent manner. The results are represented that the HAEATL produce the significant decreased the swimming time, increased the anoxia time, decreased level of biochemical parameters such as glucose and cholesterol except blood urea nitrogen, decreased level of white blood cell and red blood cell, and more significantly decreased the catalepsies score on 7th day compared to the control group. Conclusion: The above valuable animal study, we concluded that the HAEATL showing significantly affect compeer to the disease control group on neuroprotective activity.

scholarly journals ADDITIVE NEUROPROTECTIVE EFFECT OF 3-HYDROXYPYRIDINE DERIVATIVES AND HUMAN ERYTHROPOETIN ANALOGUE ON A HEMORRHAGIC STROKE MODEL IN RATS

2020 ◽  
Vol 8 (3) ◽  
pp. 169-180
Author(s):  
P. D. Kolesnichenko ◽  
O. V. Scheblykina ◽  
N. I. Nesterova ◽  
D. V. Scheblykin ◽  
A. V. Nesterov ◽  
...  
Keyword(s):  
Survival Rate ◽  
Hemorrhagic Stroke ◽  
Neuroprotective Effect ◽  
Cerebrovascular Disorders ◽  
Neuroprotective Activity ◽  
Control Group ◽  
Stroke Model ◽  
Combined Use ◽  
Perifocal Edema ◽  
The Brain

The correction of free radical oxidation processes is one of the most promising strategies of neuroprotection in acute cerebrovascular disorders.The aim of the study is an experimental study of the neuroprotective effects of 3-hydroxypyridine and erythropoietin derivatives, as well as their combined use.Materials and methods. The study was performed on 109 male Wistar rats. The neuroprotective effect of the substances was studied on a hemorrhagic stroke model. The study drugs were administered to the animals intraperitoneally. Carbamylated darbepoetin was administered three times in advance at the dose of 100 µg/kg within intervals of 3 days, the last injection took place 1 hour before the operation (the total dose was 300 mg/kg). Etoxidol was administered once 1 hour before the surgery at the dose of 50 mg/kg. The survival rate, behavioral features and the state of the animals on the 1st, 3rd, 7th and 14th days were recorded, and the morphological assessment of the brain was carried out.Results. The investigated substances had a positive effect on both the survival rate of the animals during the first day and on the 14th day. The best survival rates on the 14th day were recorded in the group of a combined use of ethoxydol and carbamylated darbepoetin (75%). Thus, in this group of rats, a faster recovery of neurological disorders was already distinguished from the first day on. By the 7th day, more than 50% of the rats receiving the combination of the studied drugs, had had a slight neurological deficit (up to 3 points on the McGrow scale); by the 14th day there had been only minor changes in the neurological status in the rats of this group. A pronounced neuroprotective effect of the combination of 3-hydroxypyridine and erythropoietin derivatives has been confirmed by a histological examination of brain slices – a more rapid decrease in the size of perifocal edema and microcirculation disorders, less damage to neurons and glial elements, and faster processes of resorption and organization of hemorrhage. A macroscopic examination of the brain sections stained with triphenyltetrazolium chloride of the dying rats, showed that perifocal necrosis had been the main cause of high mortality in the control group after the 3rd day.Conclusion. As a result of the experiment, the nephroprotective effect of the studied derivatives of 3-hydroxypyridine and erythropoietin has been proved. Moreover, the combination of these drugs has shown a greater neuroprotective activity than their isolated use. The additive effect of these drugs was due to their action mechanism resulting from the synergism of various structures and components of the cells.

scholarly journals FORMULASI SEDIAAN KRIM ANTI LUKA BAKAR DARI EKSTRAK AIR DAGING DAUN ALOE VERA

Jurnal Kimia ◽  
2020 ◽  
pp. 37
Author(s):  
P. O. Samirana ◽  
N. W. Satriani ◽  
P. R. Harfa ◽  
S. P. P. Dewi ◽  
C. I. S. Arisanti
Keyword(s):  
Aloe Vera ◽  
Water Extract ◽  
Ash Content ◽  
Water Soluble ◽  
Soluble Extract ◽  
Meat Extract ◽  
Chemical Screening ◽  
Extract Content ◽  
Water Soluble Extract

Aloe vera (Aloe vera) is a plant that is empirically often used to heal burns. Aloe vera leaf meat water extract contains saponins and flavonoids, in addition it also contains tannins and polyphenols. This research was conducted to determine whether the extracted water of Aloe vera leaf meat had met the parameters of extract quality standards so that it could be used in formulations. The steps taken are the extraction of aloe vera leaf meat with the method of infundation maceration, standardization of Aloe vera meat water extraction including testing the determination of drying shrinkage, total ash content, determination of ash content which is insoluble in acid, determination of the essence of water soluble extract, determination of the extract soluble in ethanol and chemical screening, identification with FT-IR, preparation of cream preparations, evaluation of cream preparations. Aloe vera leaf meat extract was obtained by infudation technique. Tests for drying drying extract produced 26.33%, total ash content of 1.3%, water soluble extract content of 11.9% and ethanol soluble extract content of 12.01%, total flavonoid content of 2.9%.   Keywords: Aloe Vera, Formulation, Cream, Burns.

scholarly journals Neuroprotective Effect of the Ginsenoside Rg1 on Cerebral Ischemic Injury In Vivo and In Vitro Is Mediated by PPARγ-Regulated Antioxidative and Anti-Inflammatory Pathways

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yang Li ◽  
Yue Guan ◽  
Ying Wang ◽  
Chun-Lei Yu ◽  
Feng-Guo Zhai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Ginsenoside Rg1 ◽  
Cerebral Ischemia Reperfusion ◽  
Cerebral Ischemia Reperfusion Injury ◽  
Cerebral Ischemic ◽  
Neuroprotective Action

The ginsenoside Rg1 exerts a neuroprotective effect during cerebral ischemia/reperfusion injury. Rg1 has been previously reported to improve PPARγexpression and signaling, consequently enhancing its regulatory processes. Due to PPARγ’s role in the suppression of oxidative stress and inflammation, Rg1’s PPARγ-normalizing capacity may play a role in the observed neuroprotective action of Rg1 during ischemic brain injury. We utilized a middle cerebral artery ischemia/reperfusion injury model in rats in addition to an oxygen glucose deprivation model in cortical neurons to elucidate the mechanisms underlying the neuroprotective effects of Rg1. We found that Rg1 significantly increased PPARγexpression and reduced multiple indicators of oxidative stress and inflammation. Ultimately, Rg1 treatment improved neurological function and diminished brain edema, indicating that Rg1 may exert its neuroprotective action on cerebral ischemia/reperfusion injury through the activation of PPARγsignaling. In addition, the present findings suggested that Rg1 was a potent PPARγagonist in that it upregulated PPARγexpression and was inhibited by GW9662, a selective PPARγantagonist. These findings expand our previous understanding of the molecular basis of the therapeutic action of Rg1 in cerebral ischemic injury, laying the ground work for expanded study and clinical optimization of the compound.

Remote ischemic conditioning for acute moderate ischemic stroke (RICAMIS): Rationale and design

2019 ◽  
Vol 15 (4) ◽  
pp. 454-460
Author(s):  
Xiao-Qiu Li ◽  
Lin Tao ◽  
Zhong-He Zhou ◽  
Yu Cui ◽  
Hui-Sheng Chen ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Clinical Studies ◽  
Ischemia Reperfusion Injury ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Control Group ◽  
Open Label ◽  
Remote Ischemic Conditioning ◽  
Ischemic Conditioning ◽  
Experimental Group

Rationale A large number of basic and clinical studies have proved that remote ischemic conditioning has neuroprotective effect. For example, remote ischemic conditioning showed a neuroprotective role in cerebral ischemia-reperfusion injury model. Recent clinical studies suggested that remote ischemic conditioning may improve neurological function and reduce the risk of recurrence in ischemic stroke patients. However, there is a lack of convincing evidence for the neuroprotective effect of remote ischemic conditioning on ischemic stroke, which deserves further study. Aim To explore the efficacy and safety of remote ischemic conditioning for acute moderate ischemic stroke. Sample size estimates A maximum of 1800 subjects are required to test the superiority hypothesis with 80% power according to a one-sided 0.025 level of significance, stratified by gender, age, time from onset to treatment, National Institutes of Health Stroke Scale (6–10 vs. 11–16), degree of responsible vessel stenosis, location of stenosis, and stroke etiology. Methods and design Remote Ischemic Conditioning for Acute Moderate Ischemic Stroke is a prospective, random, open label, blinded endpoint and multi-center study. The subjects are divided into experimental group and control group randomly. The experimental group was treated with remote ischemic conditioning twice daily with 200 mmHg pressure for 10–14 days besides guideline-based therapy. The control group was treated according to the guidelines. Study outcome The primary efficacy endpoint is favorable functional outcome, defined as modified Rankin Scale 0–1 at 90 days post-randomization.

scholarly journals Experimental Study on Anti Scorpion Venom potential of Paravatadi Agada of Ayurveda in Indian Red Scorpion Venom (Mesobuthus tamulus)

2020 ◽  
Vol 11 (3) ◽  
pp. 456-465
Author(s):  
Sunil Deshmukh ◽  
Sonali Chalakh ◽  
Dhirajsing Rajput
Keyword(s):  
Lethal Dose ◽  
Treated Group ◽  
Scorpion Venom ◽  
Water Soluble ◽  
Control Group ◽  
Soluble Extract ◽  
Percentage Survival ◽  
Soxhlet Apparatus ◽  
Frequent Event ◽  
Water Soluble Extract

Background: Scorpion sting is a frequent event in tropical and subtropical countries. The objective of this study is to evaluate Efficacy of Paravatadi Agada on Indian Red Scorpion Venom (Mesobuthus Tamulus). Materials and Methods: PA was prepared as per textual reference. Water soluble extract of PA was obtained using Soxhlet apparatus. Swiss albino mice of 20-30gm were used. Lypholised venom sample of Mesobuthus tamales and Lyophilized monovalent enzymerefined immunoglobuline anti scorpion venom serum (ASV) was used.  Using lethal dose of scorpion venom (25.12 μg/g), venom neutralising property of PA extract (300mg/kg), ASV(1mg) intra-peritoneally  and PA(31mg/mice) orally. The parameter used were Mean survival time, protection fold and percentage survival of animals over the period of 24 hrs. Histopathological examinations of all mice were done. Result: Maximum protection fold is seen in ASV treated group which is 10.03 with 83.33 % survival but water soluble extract of PA also showed some protective effect against scorpion venom 7.68 with 50 % survival rate. Histopathological examination showed that PA extract, ASV and PA treated group showed less effect of scorpion venom on Heart, Liver and Kidney compared to control group in which sever histopathological manifestations are detected. Conclusion : The protection fold and survival percentage of extract of PA was  better than Powder form of PA but less than ASV but  enough significant in view of availability, safety, ease in method of preparation and cost effectiveness compared to ASV.

scholarly journals Neuroprotective Effect Of Musk On Rat Brain Ischemic Model

2020 ◽  
Author(s):  
Radnaa Gochoo ◽  
Oyuntsetseg Namsrai ◽  
Dagvatseren Begzsuren ◽  
Bat-Erdene Jargalsaikhan ◽  
Chimedragchaa Chimedtseren
Keyword(s):  
Cerebral Ischemia ◽  
Rat Brain ◽  
Neuroprotective Effect ◽  
Ischemia Reperfusion ◽  
Neuronal Cell ◽  
Neurological Deficits ◽  
Control Group ◽  
Ischemic Reperfusion ◽  
Cerebral Ischemia Reperfusion ◽  
Significant Difference

Abstract Background Stroke is leading cause of morbidity and mortality in worldwide. Despite valuable progresses in understanding neurological deficits after stroke, its therapeutic options are remaining limited. We aimed to study the neuroprotective effect of musk on cerebral ischemia/reperfusion injury model rats.Methods:In our experiment, we used 180 whore meal breed Wistar rats which weigh 180-220 g and divided these rats into 50 mg/kg of musk, 100 mg/kg of musk, 10 mg/kg of nimodipine, the ischemic-reperfusion groups by filling the midriff of the brain and take the drugs for 7 days in each group. Cerebral ischemia/reperfusion was induced in rats by temporary middle cerebral artery occlusion-reperfusion (MCAO/R) followed by treatment with musk at 50 mg/kg and 100 mg/kg doses. On days 1, 3 and 7 after MCAO/R, TGF-β, BDNF, TrkB and NGF mRNA expressions in the rat brain tissue were quantitatively analyzed using RT-PCR.Results:Musk 50 and 100 mg/kg treated groups brain stroke size were significantly decreased compared with the experimental group at 1, 3 and 7 days. Moreover, brain BDNF, TrkB, NGF and TGF-β mRNA express were not significant difference between experimental and control group. Also 3rd and 7th day, the data indicate that Musk 50 and 100 mg/kg were significantly (p<0,05) effective increasing rats brain BDNF, TrkB, NGF and TGF- β mRNA express in rats with ischemic stroke induced by MCAO/R. Conclusions: The 50 and 100 mg/kg doses of musk lead to increase neuro-protective factors BDNF, TRkB, NGF and TGF- β expression of mRNA in ischemic-reperfusion rat model. It implies that the Mongolian musk supports the neurogenesis of neuronal cell.

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