scholarly journals Ethnopharmacological Evaluation ofBreuEssential Oils fromProtiumSpecies Administered by Inhalation

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Eduardo Rodrigues da Silva ◽  
Danilo Ribeiro de Oliveira ◽  
Patrícia Dias Fernandes ◽  
Humberto Ribeiro Bizzo ◽  
Suzana Guimarães Leitão
Keyword(s):  
Essential Oils ◽  
Formalin Test ◽  
Significant Inhibition ◽  
Chemical Compositions ◽  
Static Headspace ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Test Formulation ◽  
Similar Chemical ◽  
Sedative Effects

Background.Breuis an aromatic oleoresin which has been used by Amazonian traditional communities as a remedy for headaches and migraines by burning and inhaling the smoke produced during its combustion. This study evaluated the antinociceptive and sedative activities of formulations containingbreuessential oils administered by inhalation.Methods. Five different formulations (A–E) containingbreuessential oils were evaluated for their sedative and antinociceptive activities in mice. They were delivered for 20 minutes using an inhalation chamber coupled with a nebulizer and the air inside was collected by static headspace and analyzed by GC-FID.Results. All nebulized formulations had similar chemical compositions and major compounds as the original essential oils. None of them resulted in significant increase in response time during the hot plate test. In the formalin test, Formulation E showed a significant inhibition of licking responses in the early (46.8%) and late (60.2%) phases. Formulation B was effective (36.9%) in the first phase and Formulation D (37.9%) in the second. None of the formulations presented sedative effects.Conclusion.Breuessential oils, when inhaled, may present antinociceptive and anti-inflammatory properties without sedation. Additionally, nebulization proved to be an efficient method for administration of formulations containing these essential oils.

scholarly journals Analgesic effect of intrathecally administered orexin-A in the rat formalin test and in the rat hot plate test

2002 ◽  
Vol 137 (2) ◽  
pp. 170-176 ◽  
Author(s):  
Tatsuo Yamamoto ◽  
Natsuko Nozaki-Taguchi ◽  
Tanemichi Chiba
Keyword(s):  
Analgesic Effect ◽  
Formalin Test ◽  
Hot Plate ◽  
Orexin A ◽  
Hot Plate Test ◽  
Plate Test

scholarly journals Antinociceptive and Anti-Inflammatory Activities ofTeucrium persicumBoiss. Extract in Mice

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Abdolhossein Miri ◽  
Javad Sharifi-Rad ◽  
Kaveh Tabrizian ◽  
Ali Akbar Nasiri
Keyword(s):  
Chronic Pain ◽  
Neuropathic Pain ◽  
Sciatic Nerve ◽  
Analgesic Effect ◽  
Formalin Test ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Cotton Pellet ◽  
Anti Inflammatory

Background.Therapeutic properties ofTeucriumspecies as antioxidant, antibacterial, analgesic, anticancer, diuretic, and tonic compounds have been proved earlier.Materials and Methods. In this study, the antinociceptive and anti-inflammatory effects of the aqueous extract ofTeucrium persicumon chronic pain, sciatic nerve ligation as a model of neuropathic pain, and inflammatory models were investigated by formalin, hot-plate, and cotton pellet-induced granuloma models in mice, respectively.T. persicumaqueous extracts (100, 200, and 400 mg/kg) were orally gavaged for one week. On 8th day, the time spent and the number of lickings were recorded in formalin test. Morphine and Diclofenac were used intraperitoneally as positive controls. In sciatic nerve ligated animals, as a model of neuropathic pain, doses (100, 200, and 400 mg/kg) ofT. persicumextract (TPE) were orally gavaged for 14 consecutive days. The analgesic effect of this extract was examined 14 days after sciatic nerve ligation using the hot-plate test. Controls received saline and Imipramine (40 mg/kg, i.p.) was used a positive control for neuropathic pain model.Results.In the formalin test, a week oral gavage of all TPE doses (100, 200, and 400 mg/kg) caused a significant decrease on the licking response compared to the control negative animals. In the hot-plate test, doses of 200 and 400 mg/kg showed significant analgesic effects in sciatic nerve ligated animals. Oral gavaged of TPE revealed significant analgesic effect on chronic pain in both formalin test and sciatic nerve ligated animals. The TPEs did not have any significant anti-inflammatory effects in cotton pellet-induced granuloma formation in mice.Conclusions.These results suggest that the aqueous extract fromT. persicumBoiss. produced antinociceptive effects. Its exact mechanism of action still remains indistinct.

Effects of luteolin and luteolin-morphine co-administration on acute and chronic pain and sciatic nerve ligated-induced neuropathy in mice

2017 ◽  
Vol 14 (1) ◽  
Author(s):  
Mahmoud Hashemzaei ◽  
Mina Abdollahzadeh ◽  
Mehrdad Iranshahi ◽  
Ebrahim Golmakani ◽  
Ramin Rezaee ◽  
...  
Keyword(s):  
Body Weight ◽  
Neuropathic Pain ◽  
Adverse Reactions ◽  
Formalin Test ◽  
Diclofenac Sodium ◽  
Common Condition ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Naturally Occurring ◽  
Definite Treatment

AbstractBackgroundNeuropathic pain (NP) is a common condition accompanied by nerve injury. To date, there is no definite treatment approved for this disorder. In addition, many drugs that are used for NP cause adverse reactions. Luteolin is a naturally occurring flavonoid with diverse pharmacological properties such as anti-inflammatory, antioxidant and anticancer. We sought to investigate luteolin effects on chronic, acute and neuropathic pain as well as its potential to increase morphine anti-nociceptive effects in mice.MethodsAlbino mice (20–25 g) were randomly divided into 14 groups (n=7) including morphine 1 mg/kg body weight +luteolin (5 mg/kg body weight), morphine (9 mg/kg body weight, i.p.), luteolin (2.5, 5 and 10 mg/kg body weight), imipramine 40 mg/kg body weight and normal saline (NS) (0.9 %) as vehicle and subjected to hot plate test. Formalin test was done in the following groups: NS, diclofenac sodium (10 mg/kg body weight, i.p.), morphine (9 mg/kg body weight, i.p.) and luteolin (2.5, 5 and 10 mg/kg body weight).ResultsAdministration of luteolin single dose (5 and 10 mg/kg body weight) significantly reduced neuropathic pain (ConclusionsOur results showed that luteolin alone reduces neuropathic pain. Furthermore, when co-administered with morphine 1 mg/kg body weight, luteolin potentiates morphine effects. Therefore, luteolin-morphine co-administration might be a valuable alternative for the conventional treatment.

scholarly journals Acute toxicity and central and peripheral analgesic activity of flowers and leaves essential oils of Asteriscus graveolens from South-West Algeria

2020 ◽  
Vol 10 (5-s) ◽  
pp. 115-118
Author(s):  
Keddar Youcef Benaissa ◽  
Aicha Megherbi ◽  
Mohammed El-Amin Said ◽  
Abdelfettah Benyamina ◽  
Fouzia Toumi ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Essential Oils ◽  
Analgesic Activity ◽  
Antinociceptive Effect ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Writhing Test ◽  
Pharmacological Tests ◽  
South West ◽  
Maximum Inhibition

The objective of this study is the evaluation of the central and peripheral analgesic activity of the leaves and flowers Essential Oils (EOs) of Asteriscus graveolens as well as their acute toxicity. The pharmacological tests were performed using an animal model. Acute toxicity was determined by Lorke's method. Central analgesic activity was evaluated by the hot plate test, and peripheral analgesic activity was conducted by the writhing test. The results showed that the EOs of A. graveolens from both organs are weakly toxic. For the central analgesic activity, the results revealed that leaf EO have a remarkable antinociceptive effect compared to flowers EO with a maximum latency time of the animals on the hot plate on the order of 22.5 seconds. Finally, the peripheral analgesic activity revealed a maximum inhibition of abdominal writhing of 99% for flowers EO and 96% for leaves EO. Keywords: Asteriscus graveolens, analgesic activity, toxicity. 

Chemical Compositions of the Essential Oils and Headspace Volatiles of Seseli peucedanoides Plant Parts

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1985069
Author(s):  
Goran M. Petrović ◽  
Jelena G. Stamenković ◽  
Olga P. Jovanović ◽  
Gordana S. Stojanović
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Chemical Compositions ◽  
Static Headspace ◽  
Headspace Volatiles ◽  
Plant Parts ◽  
Major Class ◽  
Germacrene D ◽  
Leaf Essential Oil ◽  
Main Components

The chemical compositions of the essential oils of Seseli peucedanoides (M.Bieb.) Koso-Pol. inflorescences and leaves, isolated by hydrodistillation, and headspace volatiles, obtained by the static headspace method, were analyzed in detail by gas chromatography (GC) and GC/mass spectrometry (MS). In total, 74 constituents were identified, representing more than 98% of the observed GC peaks. The number of identified essential oil components obtained from the inflorescences was 63 while for the leaf essential oil it was 46. A much smaller number of compounds, 26 for inflorescences and 21 for leaves, were detected in the headspace samples. In both essential oils the most abundant compounds were the same, ( E)-caryophyllene and germacrene D, only in different proportions. The main components in the headspace specimens were α-pinene and ( E)-β-ocimene with ( E)-caryophyllene and ( Z)-3-hexen-1-ol also determined in significant percentages. The major class of compounds identified in the investigated essential oils was hydrocarbon sesquiterpenes with a share of over 80%, while the most dominant class of the headspace volatiles was hydrocarbon monoterpenes, which contribute slightly less than 80% of the total.

scholarly journals Aryl Hydrocarbon Receptors in Indole Derivative Treated Mice: Neuropharmacological Perspectives

2021 ◽  
Vol 48 (1) ◽  
pp. 25-33
Author(s):  
P. Andreeva-Gateva ◽  
J. Tchekalarova ◽  
K. Kamenova ◽  
M. Strokova-Stoilova ◽  
S. Chakar ◽  
...  
Keyword(s):  
Analgesic Activity ◽  
Indole Derivative ◽  
Molecular Mechanisms ◽  
Formalin Test ◽  
Aryl Hydrocarbon Receptors ◽  
Hot Plate ◽  
Neuroprotective Effects ◽  
Hot Plate Test ◽  
Aryl Hydrocarbon ◽  
Dose Dependent

Abstract Aim/objective. When applied in pharmacological doses, the indole derivative melatonin exhibits neuroactive and neuroprotective effects. Indoles and their metabolites, such as kynurenine, are ligands of aryl hydrocarbon receptors (AhR). This study aimed to evaluate the antiepileptic and analgesic activity of melatonin and two synthetic melatonin derivatives. The possible involvement of AhR and kynurenine in their neuropharmacological effects were also tested. Methods. The tested substances were: melatonin, two melatonin derivatives bearing aryl hydrocarbon moiety with either furyl or thienyl substitute (3e and 3f), and alpha naphthoflavone (ANF), an antagonist of AhR. After intraperitoneal injection of 30, 100, or 300 mg/kg of the tested agents for seven days, male mice ICR (25-30 g) were subjected to a corneal kindling seizure model. Two tests for analgesia, i.e., the hot plate test and the formalin test, were also applied. AhR and kynurenine concentrations were evaluated in brain homogenates. Results. Substances 3e and 3f demonstrated an antiepileptic activity comparable to that of melatonin. Some analgesic activity was also shown, albeit lower than that of melatonin in equivalent doses. For melatonin and 3f treated mice, dose-dependent increases in AhR and kynurenine levels in brain homogenates were recorded. The antagonist ANF neither blocks the antiseizure activity of the tested indoles, nor demonstrated analgesic activity. Conclusion. Melatonin and the two tested melatonin-aroylhydrazone derivatives bearing either furyl or thienyl substitute exhibit antiepileptic and analgesic activity. Our results did not support the involvement of AhR in the demonstrated neurobiological activity. Further studies are needed to elucidate their exact molecular mechanisms.

scholarly journals Antinociceptive and anti-inflammatory effects of Rhodiola Rosea L. extract in rats

Folia Medica ◽  
2013 ◽  
Vol 55 (3-4) ◽  
pp. 70-75 ◽  
Author(s):  
Nina D. Doncheva ◽  
Anita St. Mihaylova ◽  
Damianka P. Getova
Keyword(s):  
Formalin Test ◽  
Heated Surface ◽  
Rhodiola Rosea ◽  
Paw Edema ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Alcohol Water ◽  
Anti Inflammatory ◽  
Rhodiola Rosea Extract

ABSTRACT BACKGROUND: Rhodiola rosea (golden root) is a unique phytoadaptagen with immunomodulatory, antioxidant, anti-inflammatory and antinociceptive activity. AIM: The aim of this study was to evaluate the antinociceptive and anti-inflammatory effects of the alcohol/water extract of Rhodiola rosea roots in rats. MATERIALS AND METHODS: Thirty male Wistar rats were used in the study. They were divided in 3 groups (n = 10), treated respectively with saline (controls), Rhodiola rosea extract 50 mg/kg bw and 100 mg/kg bw orally. The antinociceptive effect was evaluated using the hot-plate test, Randall-Sellito test and the formalin test. The hot-plate test evaluates the reaction time of rats which are dropped on a heated surface. The analgesy-meter test exerts a force increased at constant rate. In the formalin test we measured the total time spent in licking the injected paw during the early (0-10 min) and late phase (20-30 min) of test. To study anti-inflammatory effect the carrageenan-induced paw edema was used. The paw volume was measured plethysmometrically at 2, 3 and 4 hours. RESULTS: In the hot-plate test Rhodiola rosea increased in both doses the latency reaction compared with that in the controls. In analgesy-meter test Rhodiola rosea in a dose of 50 mg/kg showed a significant increase of pressure reaction compared with the controls. In the formalin test Rhodiola rosea in a dose of 100 mg/kg significantly decreased the paw licking time during the first phase. In the plethysmometer test Rhodiola rosea extract significantly reduced carrageenan-induced paw edema when compared with the saline-induced edema. CONCLUSION: The studied extract of Rhodiola rosea exhibited significant analgesic activity in all the pain models used - inhibition of thermal pain, mechanical hyperalgesia and formalin-induced pain behavior. Significant anti-inflammatory activity was observed from Rhodiola rosea extract in carrageenan induced paw edema in rats.

scholarly journals Effects of Anethole in Nociception Experimental Models

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Alessandra Mileni Versuti Ritter ◽  
Franciele Queiroz Ames ◽  
Fernando Otani ◽  
Rubia Maria Weffort de Oliveira ◽  
Roberto Kenji Nakamura Cuman ◽  
...  
Keyword(s):  
Formalin Test ◽  
Open Field Test ◽  
Antinociceptive Effect ◽  
Experimental Models ◽  
Sedative Effect ◽  
Second Phase ◽  
Hot Plate ◽  
Star Anise ◽  
Sedative Effects ◽  
Illicium Verum

This study investigated the antinociceptive activity of anethole (anethole 1-methoxy-4-benzene (1-propenyl)), major compound of the essential oil of star anise (Illicium verum), in different experimental models of nociception. The animals were pretreated with anethole (62.5, 125, 250, and 500 mg/kg) one hour before the experiments. To eliminate a possible sedative effect of anethole, the open field test was conducted. Anethole (62.5, 125, 250, and 500 mg/kg) showed an antinociceptive effect in the writhing model induced by acetic acid, in the second phase of the formalin test (125 and 250 mg/kg) in the test of glutamate (62.5, 125, and 250 mg/kg), and expresses pain induced by ACF (250 mg/kg). In contrast, anethole was not able to increase the latency time on the hot plate and decrease the number of flinches during the initial phase of the formalin test in any of the doses tested. It was also demonstrated that anethole has no association with sedative effects. Therefore, these data showed that anethole, at all used doses, has no sedative effect and has an antinociceptive effect. This effect may be due to a decrease in the production/release of inflammatory mediators.

scholarly journals Analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum

2009 ◽  
Vol 52 (5) ◽  
pp. 1115-1121 ◽  
Author(s):  
Glauciemar Del-Vechio-Vieira ◽  
Orlando Vieira de Sousa ◽  
Mariza Abreu Miranda ◽  
Luci Senna-Valle ◽  
Maria Auxiliadora Coelho Kaplan
Keyword(s):  
Essential Oil ◽  
Formalin Test ◽  
Second Phase ◽  
Hot Plate ◽  
Hot Plate Test ◽  
Plate Test ◽  
Medicinal Use ◽  
Leukocyte Mobilization ◽  
Germacrene D ◽  
Anti Inflammatory

The chemical composition, analgesic and anti-inflammatory properties of essential oil from Ageratum fastigiatum were investigated. The main compounds found in the essential oil were germacrene D, α-humulene and β-cedrene. The oil, with LD50 of 2.50 g/kg, inhibited the acetic acid-induced writhing at the dose of 200 mg/kg. In the formalin test, the oil inhibited the first phase (200 mg/kg) and the second phase (100 mg/kg and 200 mg/kg). In the hot plate test, after 30 and 60 min of treatment the doses of 100 and 200 mg/kg increased the reaction time. The antiedematogenic effect, reduction on the exudate volume and leukocyte mobilization were observed at the doses of 100 and 200 mg/kg. The results indicated that A. fastigiatum possessed the analgesic and anti-inflammatory properties that supported the popular medicinal use of the plant.

Pharmacological Activity of (R)-(+)-Pulegone, a Chemical Constituent of Essential Oils

2011 ◽  
Vol 66 (7-8) ◽  
pp. 353-359 ◽  
Author(s):  
Damião P. de Sousa ◽  
Franklin F. F. Nóbrega ◽  
Maria R. V. de Lima ◽  
Reinaldo N. de Almeida
Keyword(s):  
Essential Oils ◽  
Formalin Test ◽  
Chemical Constituent ◽  
Major Constituent ◽  
Second Phase ◽  
Analgesic Drug ◽  
Hot Plate Test ◽  
Thermal Nociception ◽  
The Central Nervous System ◽  
Reaction Latency

(R)-(+)-Pulegone is a monoterpene found in essential oils from plants of the Labiatae family. This compound is a major constituent of Agastache formosanum oil. In this study, the effect of (R)-(+)-pulegone on the central nervous system was evaluated. (R)-(+)-Pulegone caused a significant decrease in ambulation and an increase in pentobarbital-induced sleeping time in mice, indicating a central depressant effect. (+)-Pulegone also significantly increased the latency of convulsions as assessed by the pentylenetetrazole (PTZ) method. The antinociceptive properties of this monoterpene were studied in chemical and thermal models of nociception. Chemical nociception induced in the first and second phase of the subplantar formalin test was significantly inhibited by (R)-(+)-pulegone and was not blocked by naloxone. Thermal nociception was also significantly inhibited while (R)-(+)-pulegone increased the reaction latency of the mice in the hot plate test. These results suggest that (R)-(+)-pulegone is a psychoactive compound and has the profile of an analgesic drug.

Sign in / Sign up

Export Citation Format

Share Document