scholarly journals Bone Healing Improvements Using Hyaluronic Acid and Hydroxyapatite/Beta-Tricalcium Phosphate in Combination: An Animal Study

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Yen-Lan Chang ◽  
Yi-June Lo ◽  
Sheng-Wei Feng ◽  
Yu-Chih Huang ◽  
Hsin-Yuan Tsai ◽  
...  
Keyword(s):  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Control Sample ◽  
Volume Density ◽  
Parietal Bone ◽  
Control Group ◽  
Calvarial Bone ◽  
Handling Characteristics ◽  
Beta Tricalcium Phosphate ◽  
Aqueous Binder

The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0–3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.

The Role of Bone Grafts in Preventing Medication-Related Osteonecrosis of the Jaw: Histomorphometric, Immunohistochemical, and Clinical Evaluation in Animal Model

2021 ◽  
pp. 194338752110483
Author(s):  
Jonathan Ribeiro da Silva ◽  
Maria Cristina de Moraes Balbas ◽  
Caroline Águeda Corrêa ◽  
Manuella Zanela ◽  
Roberta Okamoto ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Tissue ◽  
Clinical Evaluation ◽  
Tricalcium Phosphate ◽  
Bone Grafts ◽  
Osteonecrosis Of The Jaw ◽  
Control Group ◽  
Bovine Bone ◽  
Beta Tricalcium Phosphate ◽  
Group I

Objective: To evaluate the effects of inorganic bovine bone graft (Lumina Bone, Criteria, Brazil) and beta-tricalcium phosphate (β-TCP) graft (ChronOS, Synthes, Brazil) in rats with the risk of developing post-extraction medication-related osteonecrosis of the jaw (MRONJ). Methods: Eighteen male Wistar rats weighing 350 to 450 g were induced to develop MRONJ using zoledronic acid for 5 weeks. In the sixth week, the right maxillary first molar was extracted. The animals in Group I (G1) did not receive bone grafts after tooth extraction, while Group II (G2) animals received inorganic bovine bone grafts, and Group III (G3) animals received beta-tricalcium phosphate (β-TCP) grafts. Clinical evaluation and histomorphometric and immunohistochemical analyses were performed. ANOVA and Tukey’s statistical tests were used and a level of significance was considered to be 5%. Results: In the clinical evaluation, animals from G2 and G3 did not present clinical manifestations of osteonecrosis, unlike the control group (G1) animals, which presented necrotic bone tissue exposure in all samples. In the histomorphometric evaluation, animals in G3 showed greater formation of bone tissue (66%) and less formation of bone lacuna (18%) than animals in G1 (58%/32%) and in G2 (59%/27%) ( P < 0.05). Moderate (++) immunostaining was observed in G2 and G3 for RANKL, TRAP, and OC, while G1 showed moderate (++) labeling for OC and mild (+) immunostaining for TRAP and RANKL. Conclusions: Greater formation of bone tissue and fewer bone lacunae were found in animals treated with β-TCP. In clinical evaluation, bone graft groups presented with the clinical manifestation of MRONJ and showed higher intensity of immunostaining for TRAP and RANKL. Despite the limitations of experimental animal studies, the results of this work may assist in the development of future clinical research for the prevention of MRONJ.

scholarly journals Histopathological evaluation of polycaprolactone nanocomposite compared with tricalcium phosphate in bone healing

2018 ◽  
Vol 62 (3) ◽  
pp. 385-394 ◽  
Author(s):  
Hadi Eftekhari ◽  
Alireza Jahandideh ◽  
Ahmad Asghari ◽  
Abolfazl Akbarzadeh ◽  
Saeed Hesaraki
Keyword(s):  
Bone Tissue ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Healing Process ◽  
Control Group ◽  
Time Intervals ◽  
Positive Effects ◽  
Bone Scaffolds ◽  
Modern Engineering ◽  
And Control

AbstractIntroductionIn recent years, the use of bone scaffolds as bone tissue substitutes, especially the use of such as hydroxyapatite and tricalcium phosphate, has been very popular. Today, the use of modern engineering techniques and advances in nanotechnology have expanded the use of nanomaterials as bone scaffolds for bone tissue applications.Material and MethodsThis study was performed on 60 adult male New Zealand rabbits divided into four experimental groups: the control group without any treatment, the second group receiving hydroxyapatite, the third group treated with β-tricalcium phosphate, and the fourth group receiving nanocomposite polycaprolactone (PCL) scaffold. In a surgical procedure, a defect 6 mm in diameter was made in a hind limb femur. Four indexes were used to assess histopathology, which were union index, spongiosa index, cortex index, and bone marrow.ResultsThe results showed that nanocomposite PCL and control groups always had the respective highest and lowest values among all the groups at all time intervals. The histopathological assessment demonstrated that the quantity of newly formed lamellar bone in the nanocomposite PCL group was higher than in other groups.ConclusionAll these data suggest that PCL had positive effects on the bone healing process, which could have great potential in tissue engineering and clinical applications.

scholarly journals Alteration of clot architecture using bone substitute biomaterials (beta-tricalcium phosphate) significantly delays the early bone healing process

2018 ◽  
Vol 6 (48) ◽  
pp. 8204-8213 ◽  
Author(s):  
Xin Wang ◽  
Yan Luo ◽  
Yan Yang ◽  
Baoyu Zheng ◽  
Fuhua Yan ◽  
...  
Keyword(s):  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Bone Substitute ◽  
Blood Clot ◽  
Healing Process ◽  
The Body ◽  
Beta Tricalcium Phosphate ◽  
Bone Healing Process

When a bone substitute biomaterial is implanted into the body, the material's surface comes into contact with circulating blood, which results in the formation of a peri-implant hematoma or blood clot.

Rat Calvarial Bone Regeneration by 3D-Printed Beta-Tricalcium Phosphate Incorporating MicroRNA-200c

2020 ◽  
Author(s):  
Matthew T. Remy ◽  
Adil Akkouch ◽  
Li He ◽  
Steven Eliason ◽  
Mason E. Sweat ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Bone ◽  
Beta Tricalcium Phosphate ◽  
3D Printed

scholarly journals Effect of platelet rich fibrin and beta tricalcium phosphate on bone healing. A histological study in pigs

2014 ◽  
Vol 29 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Dincer Yilmaz ◽  
Necdet Dogan ◽  
Aydin Ozkan ◽  
Metin Sencimen ◽  
Baris Eren Ora ◽  
...  
Keyword(s):  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Histological Study ◽  
Platelet Rich Fibrin ◽  
Beta Tricalcium Phosphate

Effect of Synthetic β-TCP and Bovine-Derived Hydroxyapatite Grafting on Bone Regeneration in Rats

2007 ◽  
Vol 361-363 ◽  
pp. 1261-1264
Author(s):  
C.S. Lim ◽  
S.G. Kim ◽  
Sung Chul Lim
Keyword(s):  
Bone Formation ◽  
Light Microscopy ◽  
Bone Healing ◽  
Tricalcium Phosphate ◽  
Bone Defects ◽  
Frontal Bone ◽  
Control Group ◽  
New Bone Formation ◽  
Grafting Materials ◽  
Defect Margin

We evaluated the bone healing effect of grafting with synthetic β-tricalcium phosphate (β-TCP; Cerasorb®), bovine-derived hydroxyapatite (HA; Bio-Oss®), and a mixture of β-TCP and HA in rats. Each material was grafted in prepared 8-mm frontal bone defects in 15 rats. The control group underwent surgery without any grafting materials and was examined after 4 weeks, whereas the experimental groups received grafting materials and were examined after 1, 2, and 4 weeks. After implantation, the rats were sacrificed for histomorphometric studies using light microscopy, and the data were analyzed using analysis of variance. Considerable inflammation and fibrosis were observed after 1 and 2 weeks in all experimental groups, whereas the inflammation was reduced and fibrosis was stabilized after 4 weeks. New bone formation was observed at the defect margin. Statistically, there was no difference in new bone formation among the three experimental groups. In conclusion, there was no difference in new bone formation using Bio-Oss®, Cerasorb®, and a mixture of Bio-Oss® and Cerasorb®.

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