scholarly journals Composition Analysis and Inhibitory Effect ofSterculia lychnophoraagainst Biofilm Formation byStreptococcus mutans

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Yang Yang ◽  
Bok-Im Park ◽  
Eun-Hee Hwang ◽  
Yong-Ouk You
Keyword(s):  
Biofilm Formation ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Significant Inhibition ◽  
Control Group ◽  
Composition Analysis ◽  
Phytochemical Analysis ◽  
Chinese Drug ◽  
Nutrient Contents ◽  
Ethanol Extracts

Pangdahai is a traditional Chinese drug, specifically described in the Chinese Pharmacopoeia as the seeds ofSterculia lychnophoraHance. Here, we separatedS. lychnophorahusk and kernel, analyzed the nutrient contents, and investigated the inhibitory effects ofS. lychnophoraethanol extracts on cariogenic properties ofStreptococcus mutans, important bacteria in dental caries and plaque formation. Ethanol extracts ofS. lychnophorashowed dose-dependent antibacterial activity againstS. mutanswith significant inhibition at concentrations higher than 0.01 mg/mL compared with the control group (p<0.05). Furthermore, biofilm formation was decreased byS. lychnophoraat concentrations > 0.03 mg/mL, while bacterial viability was decreased dose-dependently at high concentrations (0.04, 0.08, 0.16, and 0.32 mg/mL). Preliminary phytochemical analysis of the ethanol extract revealed a strong presence of alkaloid, phenolics, glycosides, and peptides while the presence of steroids, terpenoids, flavonoids, and organic acids was low. TheS. lychnophorahusk had higher moisture and ash content than the kernel, while the protein and fat content of the husk were lower (p<0.05) than those of the kernel. These results indicate thatS. lychnophoramay have antibacterial effects againstS. mutans, which are likely related to the alkaloid, phenolics, glycosides, and peptides, the major components ofS. lychnophora.

scholarly journals HYPOGLYCEMIC AND HYPOLIPIDEMIC EFFECTS OF ANNONA MURICATA L. LEAF ETHANOL EXTRACT

2017 ◽  
Vol 9 (3) ◽  
pp. 170 ◽  
Author(s):  
E. Sovia ◽  
W. Ratwita ◽  
D. Wijayanti ◽  
D. R. Novianty
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Total Cholesterol ◽  
Ethanol Extract ◽  
Normal Group ◽  
Islet Of Langerhans ◽  
Control Group ◽  
Phytochemical Analysis ◽  
Annona Muricata ◽  
Cholesterol Levels

Objective: The aim of this study was to investigate hypoglycemic and hypolipidemic effects of Annona muricata leaf ethanol extract. This study also investigated phytochemical analysis of the extract and improvement in the islet of Langerhans.Methods: Hypoglycemic and hypolipidemic effects were evaluated using alloxan induced diabetic rats. Twenty-five Wistar rats devided into five groups, that are normal group, control group that induced by alloxan 125 mg/kg body weight and given CMC (carboxymethyl cellulose), and three treatment group that induced by alloxan and given Annona muricata leaf ethanol extract (AMLEE) with 50, 100 and 200 mg/kg body weight. Blood glucose and total cholesterol levels were measured before and after alloxan induction, and 21 d after AMLEE treatment. At the end of the study, all of the animals experiment were sacrificed for histopathological examination.Results: Phytochemical analysis revealed that flavonoids, tannins, saponins, phytosterols and phenols were present in ethanol extract of Annona muricata L. leaf. At the end of the study, blood glucose and total cholesterol levels in all AMLEE treated group were decreased significantly (p<0.05). Final blood glucose level in the groups that given AMLEE 50 mg/kg body weight (86.7±14.6 mg/dl) was almost the same with that of the normal group (91.25±28.38 mg/dl). The result of the histopathological examination is not showed an improvement of the islet of Langerhans in AMLEE treated groups.Conclusion: In conclusion, the ethanol extract of Annona muricata L. leaf have hypoglycemic and hypolipidemic effects. However, there was no improvement in the islet of Langerhans damage.

scholarly journals EFFECT OF DIETARY SUPPLEMENTATION OF ETHANOL EXTRACT OF CHIVES AND GINGER ON THE PRODUCTIVITY AND HEALTH PERFORMANCE OF BROILERS

2020 ◽  
Vol 129 (3C) ◽  
Author(s):  
Phan Vu Hai ◽  
Pham Hoang Son Hung ◽  
Ho Thi Dung ◽  
Le Minh Đuc ◽  
Đinh Thi Thuy Khuong ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Ethanol Extract ◽  
Efficiency Index ◽  
Feed Consumption ◽  
Control Group ◽  
Small Intestinal Mucosa ◽  
Growth Promoters ◽  
Health Performance ◽  
Ethanol Extracts ◽  
Dietary Treatments

This study was to evaluate the effects of supplementation of ethanol extracts of chive (CE) and ginger (GE), and their combination (CG) in diets of broiler chickens on their growth, health performance and economic efficiency. Totally 180 male Egyptian broilers from one-day age were randomly assigned to 6 dietary treatments consisting of 3 replicates of 10 chicks each, of which in the diet of the control group (CT) neither ginger nor chive preparation were introduced, meanwhile the diets of birds in the 5 trial groups (CE1, CE2, GE1, GE2 and CG) were supplemented with 0.5% CE, 1% CE, 0.5% GE, 1% GE and 0.5% CE plus 0.5% GE preparations, respectively. In general, supplementation of CE and GE did not significantly affect feed consumption, mortality, performance efficiency index (PEI), and respiratory syndrome in chickens. However, the herb extract preparations could reduce the incidence of diarrhea in broiler chickens during five to-ten-week age periods. Microscopic examination of small intestinal mucosa showed that the heights of villi and the depths of crypts of chicks in the CE2 treatment as well as the diameters of villi of chicks in the CG treatment were significantly (p < 0.05) higher than in the others. In general, supplementation of 1% CE and the combination of 0.5% CE and 0.5% GE preparations improved the growth performance of broilers, and the combination can be an alternative to antibiotics as growth promoters in feeding chicken.

scholarly journals Antidiabetic Activities and Identification of Chemical Compound from Samama (Anthocephalus macrophyllus (Roxb) Havil)

2018 ◽  
Vol 18 (1) ◽  
pp. 66 ◽  
Author(s):  
Laela Nur Anisah ◽  
Wasrin Syafii ◽  
Gustan Pari ◽  
Rita Kartika Sari
Keyword(s):  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Antidiabetic Activity ◽  
Isopropyl Myristate ◽  
Phytochemical Analysis ◽  
Triterpenoid Saponin ◽  
Ic50 Value ◽  
High Contribution ◽  
Ethanol Extracts

Samama (Anthocephalus macrophyllus) is one of a pioneer indigenous fast growing species in Indonesia which have been used as traditional medicine for various diseases. The objectives were to determine the yield extract, to analyze their antidiabetic activity by inhibition assay for α-glucosidase enzyme activity and chemical analysis with GCMS. Extraction of leaves, bark and wood samples were done by using ethanol 95%. Fractionation the most active  ethanol extract was conducted by using n-hexane and ethyl acetate. The yield of ethanol extracts in leaves, bark and wood were 13.90%, 12.87%, and 2.18% respectively. Based on antidiabetic activity assay, the bark ethanol extract was the most active extract by the IC50 value of 5.86 μg mL-1. Phytochemical analysis on bark ethanol extracts showed that they contained flavonoid, quinon, triterpenoid, saponin and tannin which were assumed have high contribution in antidiabetic activities. The result of fractionation ethanol extract bark showed that the ethyl acetate fraction was the most active fraction (IC50 6.82 μg mL-1). GCMS analysis indicated the presence of dominant phenolic compounds such as pyrocatechol, antiarol, isopropyl myristate and phenol in which were suspected have antidiabetic activity. These results strongly suggested that ethyl acetate fraction of Samama bark was a potential natural source for antidiabetic agents.

scholarly journals Plantain Herb Extracts significantly attenuate the quorum sensing-controlled virulence factors and inhibit biofilm formation in Pseudomonas aeruginosa PAO1

2019 ◽  
Vol 78 ◽  
pp. 01004
Author(s):  
Shan Li ◽  
Jiangning Yao ◽  
Haoming Li
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Virulence Factors ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Significant Inhibition ◽  
Control Group ◽  
Produce Cell ◽  
Group B ◽  
Herb Extracts

Pseudomonas aeruginosa is a Gram-negative organism that can survive under harsh conditions, and it is also an opportunistic pathogen that can produce cell-associated extracellular virulence factors. Several of these virulence factors have been demonstrated to be regulated by quorum sensing (QS). Plantain Herb has been used as antibacterial agents for many centuries in China. In this study, we analyzed Plantain Herb Extracts (PHE) at the concentration of 16 μg/mL (Group A, MIC), 8 μg/mL (Group B, 1/2 MIC) and 4 μg/mL (Group C, 1/4 MIC) for inhibition of the virulence factors production and biofilm formation in P. aeruginosa PAO1. The virulence factors included pyocyanin, rhamnolipids, protease and alginate. PHE showed significant inhibition of virulence factors as compared to the control group without interfering its growth. Thus, PHE might be a potent QS inhibitor and anti-biofilm agent in the treatment of Pseudomonas aeruginosa infections.

Anti-Tumor Activity of Polysaccharides Isolated from Patrinia scabra Bunge on U14 Cervical Carcinoma Bearing Mice

2009 ◽  
Vol 37 (05) ◽  
pp. 933-944 ◽  
Author(s):  
Wen-Zong Lu ◽  
Guo-Xia Geng ◽  
Qing-Wang Li ◽  
Jian Li ◽  
Fu-Zhu Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Serum Alkaline Phosphatase ◽  
Inhibitory Effect ◽  
Serum Lactate ◽  
Significant Inhibition ◽  
Serum Lactate Dehydrogenase ◽  
Control Group ◽  
Tumor Tissues ◽  
Polymerase Chain ◽  
Anti Tumor Activity

The aim of our study was to investigate the effect of Patrinia scabra Bunge polysaccharide (PSB-P2) on cervical cancer cell (U14)-bearing mice. The tumor weight of mice treated with PSB-P2 (40, 80 mg/kg b.w.) was significantly lower than that of the control group and serum lactate dehydrogenase (LDH) activity was decreased, while serum alkaline phosphatase (AKP) level was only changed slightly. Meanwhile, the number of apoptotic tumor cells was significantly increased in the mice by the treatment of PSB-P2 (40, 80 mg/kg b.w.). At the same time, cell cycle analysis showed the accumulation of tumor cells in the G0/G1 phase and a relative decrease in the S phase. On the other hand, using the reverse transcription-polymerase chain reaction (RT-PCR) assay, PSB-P2 (40, 80 mg/kg b.w.) showed the up-regulation of p53 and Bax, and significant inhibition of Bcl-2 in tumor tissues. It suggests a possible mechanism of the inhibitory effect of PSB-P2 on tumor growth.

scholarly journals Effect of Annona muricata L. Leaf Ethanol Extract in CCl4 Hepatitis Rat Model

2021 ◽  
Vol 8 (2) ◽  
Author(s):  
Kuswinarti Kuswinarti ◽  
Ilham Asrori Azka ◽  
Dwi Prasetyo
Keyword(s):  
Cell Membrane ◽  
Intact Cell ◽  
Antioxidant Properties ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Negative Control ◽  
Significant Inhibition ◽  
Control Group ◽  
Annona Muricata ◽  
Anti Oxidant

Background: Liver can be damaged by various factors, including the oxidative stress mechanism. This damage can be inhibited by the application of anti-oxidant. The Annona muricata L. leaf is known to have antioxidant properties. This study aimed to explore the effect of Annona muricata L. leaf extract against hepatitis in rats.Methods: An experimental study was performed on 25 male Wistar rats that were given Annona muricata L. leaf ethanol extract at 200 mg/kgBW, 400 mg/kgBW, and 600 mg/kgBW for eight days. The CCl4 was administrated on the eighth day and the rats were laparotomized on the next day. The average number of normal hepatocytes was counted in one field of view. This study was conducted at the laboratory of Pharmacology and Therapy of the Faculty of Medicine, Universitas Padjadjaran in September–October 2012.Results: The negative control group showed an intact cell membrane, homogenous cytoplasm, and round nucleus, whereas the positive control group showed necrosis marked by a ruptured cell membrane, vacuolated cytoplasm, and degenerated nucleus. The average number of normal hepatocytes counted in the negative control and the positive group was 404.91±34.09 cells and 23.16±7.84 cells, respectively. In the treatment group, the normal hepatocytes were 95.91±20.96 cells (200 mg/kgBW), 74.53±13.59 cells (400 mg/kgBW) and 46.84±8.46 cells (600 mg/kgBW). Statistically significant inhibition was observed (p<0.05).Conclusions: The Annona muricata L. leaf ethanol extract can inhibit liver tissue damages in hepatitis model rats, possibly due to the presence of acetogenins as an anti-oxidant agent.

scholarly journals UJI EFEK ANTELMINTIK EKSTRAK ETANOL DAUN PINANG YAKI (Areca vestiaria) TERHADAP CACING GELANG (Ascaris lumbricoides) SECARA IN VITRO

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 457
Author(s):  
Tirza Roring ◽  
Herny E. I. Simbala ◽  
Edwin De Queljoe
Keyword(s):  
Cell Membrane ◽  
Ascaris Lumbricoides ◽  
Endemic Species ◽  
Ethanol Extract ◽  
Control Group ◽  
Whitney Test ◽  
Post Test ◽  
Ethanol Extracts ◽  
Anthelmintic Effect

ABSTRACT Pinang Yaki (Areca vestiaria) is an endemic species of Sulawesi palm, which has unique characteristics that have been used as medicine , to treat various. Ethanol extracts of pinang yaki leaves contains tannin compounds that can inhibit the work of enzymes, interfere with digestive metabolic processes, and damage the worm cell membrane. This study aims to determine the anthelmintic effect of ethanol extract of pinang yaki leaves obtained from the Tomohon area against roundworms (Ascaris lumbricoides). The sample was extracted by maceration method using ethanol solvent. The test used ethanol extracts f pinang yaki leaves with a concentration of 5%, 10%, and 20%, respectively. The worms were incubated at a temperature of 37ºC, and examined for 24 hours at 6 hour intervals. The number of lysis worms and paralysis was recorded every 6 hours and the data were analyzed using the Kruskal Wallis test and continued using the Mann Whitney test. The results showed an anthelmintic effect of ethanol extracts of pinang yaki leaves on roundworms (Ascaris lumbricoides) in vitro.Keywords         : Anthelmintic, Ethanol extracts of areca leaf, Areca vestiaria, Ascaris lumbricoides  ABSTRAK Pinang Yaki (Areca vestiaria) merupakan jenis palem endemik Sulawesi yang memiliki karakteristik yang unik telah dimanfaatkan sebagai obat, untuk mengobati berbagai penyakit. Ekstrak etanol daun pinang yaki mengandung senyawa tanin yang dapat menghambat kerja enzim, mengganggu proses metabolisme pencernaan, serta merusak membran sel cacing. Penelitian ini bertujuan untuk mengetahui adanya efek antelmintik dari ekstrak etanol daun pinang yaki yang diperoleh dari daerah Tomohon terhadap cacing gelang (Ascaris lumbricoides). Sampel diekstraksi dengan metode maserasi menggunakan pelarut etanol. Metode penelitian ini ialah eksperimen laboratorium dengan desain post test only control group. Pengujian menggunakan ekstrak etanol daun pinang yaki dengan konsentrasi 5%, 10%, dan 20%. Cacing diinkubasi pada suhu 37ºC, dan diteliti selama 24 jam dengan interval waktu 6 jam. Jumlah cacing lisis dan paralisis dicatat setiap 6 jam dan selanjutnya data dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan menggunakan uji Man Whitney. Hasil penelitian menunjukkan adanya efek antelmintik dari ekstrak etanol daun pinang yaki terhadap cacing gelang (Ascaris lumbricoides) secara in vitro. Kata kunci      : Antelmintik, Ekstrak Etanol Daun Pinang Yaki, Areca vestiaria, Ascaris lumbricoides

scholarly journals The Effect of Haramounting Leaf Ethanol Extract (Rhodomyrtus tomentosa (Aiton) Hassk.) on the Number of Leukocyte Type and Histology of Mice Pulmo (Mus Musculus L.) Exposed to Electronic Cigarette

2019 ◽  
Vol 7 (11) ◽  
pp. 1750-1756 ◽  
Author(s):  
Syafruddin Ilyas ◽  
Finn Murdella ◽  
Salomo Hutahaean ◽  
Putri Cahaya Situmorang
Keyword(s):  
Ethanol Extract ◽  
Electronic Cigarette ◽  
Control Group ◽  
Male Mice ◽  
Treatment Groups ◽  
Anatomy Laboratory ◽  
Leukocyte Counts ◽  
Ethanol Extracts ◽  
North Sumatra ◽  
Rhodomyrtus Tomentosa

BACKGROUND: Haramonting leaves have a large antioxidant activity as inhibitors oxidation and form non-free radical compounds that are not reactive and relatively stable. Electronic smoking is a choice who slowly want to quit conventional smoking.AIM: This research aimed to determine the effect of ethanol extract on hard mounting leaves on leukocyte counts and histology Pulmo on male mice exposed to an electronic cigarette.METHODS: The samples used in this study were healthy male mice aged 8-11 weeks and weight of 25-30 g as many as 25 individuals and ethanol extracts of hard mounting leaves with a dose of 100 mg/kgBW, 200 mg/kgBW and 300 mg/kgBW. Blood tests were carried out at the Health Laboratory of North Sumatra Office, and the preparation of Pulmo histology was carried out at the Pathology and Anatomy Laboratory of Adam Malik General Hospital MedanRESULT: There were significant differences between the control group and all of the treatment groups in the number of lymphocytes, neutrophils, basophils and eosinophils. There was a tendency that electronic cigarette smoke could cause damage to pulmo tissue.CONCLUSION: The results of this study indicate that the ethanol extract of hard mounting leaves could help the immune system in mice exposed to an electronic cigarette.

scholarly journals ANTIFUNGAL ACTIVITIES OF ETHANOL EXTRACT OF ROSEMARY LEAF (Rosemarinus officinalis L.) AGAINST Candida albicans

2020 ◽  
Vol 2 (1) ◽  
pp. 28
Author(s):  
Dewa Ayu Made Adnya Swari ◽  
I Wayan Martadi Santika ◽  
I Gusti Made Aman
Keyword(s):  
Candida Albicans ◽  
Antifungal Activity ◽  
Rosmarinic Acid ◽  
Extraction Method ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Rosmarinus Officinalis ◽  
Control Group ◽  
Rosmarinus Officinalis L

Background: This study was a preliminary study aimed to investigating the in vitro activity of rosmarinic acid contained on Rosemary leaves (Rosemarinus officinalis L.) on the Candida albicans. Rosmarinic acid can be an inhibitor of the RTPase enzyme in the fungus Candida albicans. Objective: This study is expected to provide information in the development of science about the antifungal activity of ethanol extract of Rosemary leave (Rosmarinus officinalis L.) againsts C.albicans. The recent research was an laboratory experimental. Experiments on an ethanol extract of Rosemary leaves (Rosemarinus officinalis L.) and post-test design of the control group. Methods: The extraction method used in this study was a multilevel extraction method. The antifungal activity testing method uses discs and MIC methods. Replication was done six times at two concentration namely, 80% (b/v) and 100% for each measurement. Results: The data analysis was carried out using SPSS with 95% of confidence level with the Kruskal Wallis analysis then completed with the Mann Whitney U test. The results of the MIC experiment showed all the various concentrations of ethanol extract performed Candida albicans growth on the surface of the media. Extract with 80% (b/v) concentration showed greater antifungal activity. Furthermore the MIC was shown by 80% (b/v) concentration of the ethanol extract of Rosemary leaves against Candida albicans. The test results of the antifungal activity of the Rosemary leaf ethanol extract showed the presence of inhibitory diameter in the ethanol extract of the Rosemary leaf concentration of 80% (b/v) with a mean ± SD of 10.6 ± 0.57735 and RSD of 5.774%. Conclusion: The ethanol extract of Rosemary leaves (Rosmarinus officinalis L.) has an inhibitory effect on the fungus Candida albicans at a concentration of 80% (b / v) Keywords: Antifungal activity, Candida albicans, Rosemary, RTPase, ethanol extract

UHPLC, ATR-FTIR analysis of Nymphoides indica rhizome extract and determination of antioxidant & antibiofilm potential

2021 ◽  
pp. 1-10
Author(s):  
Muhammad Hanif ◽  
Muzammil Ahmad Khan ◽  
Abdul Rafey ◽  
Imran Aziz ◽  
Mohsin Ali Khan ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Biofilm Formation ◽  
Biological Activities ◽  
Significant Inhibition ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Antioxidant Assay ◽  
Nymphoides Indica

The aim of the study was to perform comprehensive phytochemical analysis, UHPLC, ATR-FTIR profiling and evaluation of various biological activities of N. Indica rhizome. The phytochemical analysis indicated presence of alkaloids, saponins, and triterpenes. In the antioxidant assay, the methanolic (IC50 40.3±0.04) and chloroform fractions (IC50 40.05±0.21) showed highest DPPH inhition. Like wise, methanolic fraction showed highest FRAP value (756.2±0.06) followed by chloroform (225.0±0.04) and ethyl acetate fractions (193.0±0.21). In the antimicrobial assays, chloroform (MIC <  0.156 mg/ml) and methanol fractions (MIC 0.625 mg/ml) fractions showed significant inhibition of Klebsiella pneumoniae. Also a significant antibiofilm of biofilm formation was recorded by chloroform (IC50 1.73 mg/ml) and ethyl acetate fractions (IC50 1.76 mg/ml). It was concluded that the N. Indica rhizome posess antioxidant, antimicrobial and antibiofilm potential, that may be attributed to high flavonoid contents.

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