scholarly journals Micropropagation, Micromorphological Studies, andIn VitroFlowering inRungia pectinataL.

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Developmental Changes ◽  
Bud Break ◽  
Ms Medium ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Culture Protocol ◽  
Important Medicinal Plant

A tissue culture protocol was developed for an important medicinal plantRungia pectinataL. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2solution. Murashige and Skoog (MS) medium was used to establish the cultures ofR. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L−16-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded. The shoots were further multiplied using MS medium augmented with 0.5 mg L−1each of BAP and kinetin (Kin) + 0.1 mg L−1indole-3 acetic acid (IAA). Maximum 13.2 shoots per explant with 5.2 cm length were observed. All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1indole-3 butyric acid (IBA).In vitroflowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod. The plantlets were hardened in the greenhouse for two months and finally transferred to the field. The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots underin vitroconditions.

scholarly journals In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Jyotsna Sharma ◽  
◽  
Anuja Koul ◽  
Savita Sharma ◽  
Raju Shankarayan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Organogenesis ◽  
Germplasm Conservation ◽  
Ms Medium ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Shoot Tip Explants ◽  
Indole 3 Acetic Acid ◽  
Important Medicinal Plant

An efficient micropropagation protocol facilitates successful conservation and improvement of Nanorrhinum ramosissimum (Wall.) Betsche by biotechnological means. Shoot tip explants exhibited optimal organogenic response when inoculated on half-strength(1/2) Murashige and Skoog (MS) medium supplemented with kinetin (KN) and indole-3-acetic acid (IAA) (0.5 mg/L each). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; 7.9 cm shoot length). The regenerated shoots formed roots; however, the best rooting frequency (87%) was achieved on half-strength MS medium containing only IAA (0.5 mg/L). Four-week-old in vitro plantlets were acclimatized with 95% survival under greenhouse conditions. The regeneration protocol developed in this study can be utilized for germplasm conservation of this elite traditional medicinal plant.

scholarly journals In vitro clonal propagation of Nyctanthes arbortristis Linn. − a medicinal tree

2008 ◽  
Vol 34 (No. 2) ◽  
pp. 84-89 ◽  
Author(s):  
R. Rout G ◽  
A. Mahato ◽  
K. Senapati S
Keyword(s):  
Basal Medium ◽  
Shoot Multiplication ◽  
Soil Condition ◽  
Ms Medium ◽  
Medicinal Tree ◽  
Axillary Meristems ◽  
In Vitro Clonal Propagation ◽  
Indole 3 Acetic Acid ◽  
Important Medicinal Plant

Rapid shoot multiplication of Nyctanthes arbortristis was achieved from axillary meristems on Murashige and Skoog (MS) basal medium supplemented with 1.0−1.5 mg/l 6-benzyladenine (BA), 50 mg/l adenine sulfate (Ads) and 3% (m/v) sucrose. Inclusion of indole-3-acetic acid (IAA) in the culture medium along with BA + Ads promoted a higher rate of shoot multiplication. Maximum mean number of microshoots per explant (6.65) was achieved on the MS medium supplemented with 1.5 mg/l BA, 50 mg/l Ads and 0.1 mg/l IAA after 4 weeks of culture. The elongated shoots rooted within 13 to 14 days on ½ strength MS medium supplemented either with indole-3-butyric acid (IBA), IAA or naphtylacetic acid (NAA) with 2% sucrose. Maximum percentage of rooting was obtained on medium having 0.25 mg/l IBA, 0.1 mg/l IAA and 2% sucrose. About 70% of rooted plantlets survived in the greenhouse. The in vitro raised plants were grown normally in the soil condition. This result will facilitate the conservation and propagation of the important medicinal plant.

scholarly journals High-frequency Direct Somatic Embryogenesis and Plantlet Regeneration from Leaves Derived from In Vitro-Germinated Seedlings of a Coffea arabica Hybrid Cultivar

HortScience ◽  
2016 ◽  
Vol 51 (9) ◽  
pp. 1148-1152 ◽  
Author(s):  
Jane Kahia ◽  
Margaret Kirika ◽  
Hudson Lubabali ◽  
Sinclair Mantell
Keyword(s):  
Tissue Culture ◽  
High Frequency ◽  
Somatic Embryos ◽  
Coffea Arabica ◽  
Alternative Methods ◽  
Direct Somatic Embryogenesis ◽  
Ms Medium ◽  
Embryogenic Cultures ◽  
Half Strength

Breeding work carried out during the period 1971–85 by the Coffee Research Institute, Ruiru, Kenya resulted in the release of a new improved hybrid Coffea arabica named Ruiru 11. The cultivar combines resistance to coffee berry disease (CBD) and leaf rust, with high yield and good cup quality attributes. The propagation by F1 hybrid seeds production, cuttings, and tip grafting do not produce enough planting materials. There was a need to explore alternative methods and tissue culture offers potential options. The objective of the study was to evaluate the effect of explant sources and cytokinins on induction and regeneration of somatic embryos. Eight different explants were cultured on half-strength Murashige and Skoog (MS) medium supplemented with 10 µm benzylaminopurine (BAP). The effect of kinetin, N6-(2-isopentyl) adenine (2iP) evaluated at (0, 0.5, 5, or 25 µm) or thidiazuron (TDZ) (0, 0.5, 1.0, or 5 µm) added in separate experiments was also evaluated. The percentage of embryogenic cultures and the numbers of embryos per explant were determined after 3 months’ culture. The explant type had a significant effect (P > 0.05) on the induction of somatic embryos. Explants from in vitro-germinated seedlings produced the highest embryogenic cultures (90%) and the highest mean number of embryos (19.36) per explant. Cytokinins strongly enhanced induction and regeneration of somatic embryos. TDZ at 1 µm produced the highest embryogenic cultures (100%) and the highest mean number of embryos (24.2). The embryos were germinated on half-strength MS medium without any hormones. A high (98%) survival rate of the regenerated plantlets was recorded over all the treatments in the greenhouse. This is the first report on induction of high-frequency direct somatic embryos from coffee juvenile tissues. This is of great significance in tissue culture and indeed molecular biology manipulations because it allows regeneration of coffee from several explants.

scholarly journals Micropropagation and Reintroduction of the Endemic Mammillaria mathildae (Cactaceae) to Its Natural Habitat

HortScience ◽  
2010 ◽  
Vol 45 (6) ◽  
pp. 934-938 ◽  
Author(s):  
Oscar García-Rubio ◽  
Guadalupe Malda-Barrera
Keyword(s):  
Acetic Acid ◽  
Water Availability ◽  
Wild Population ◽  
Natural Habitat ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Experimental Plot ◽  
Free Media ◽  
Indole 3 Acetic Acid

A rapid shoot multiplication protocol was established for the endangered cactus Mammillaria mathildae to reintroduce it to its natural habitat. In vitro-germinated seedlings were used as the source of explants. Three explant sources (apical, lateral, and basal excised from in vitro-germinated seedlings) were tested. Shoot multiplication was induced in Murashige and Skoog (MS) medium supplemented with different 6-benzylaminopurine/indole-3-acetic acid (BA/IAA) combinations (0, 22.19, 44.39 and 0, 1.43, 2.85, 5.71, respectively). Explants developed abundant callus in the presence of any BA/IAA concentration, whereas hormone-free media produced 0.59 ± 0.11 new shoots (with a 41% callus development) from basal explants. Apical and lateral explants produced 1.14 ± 0.07 and 4.09 ± 0.13 new shoots, respectively, without callus generation. Plantlets originating from lateral explants developed a vigorous rooting system after 2 months growing on MS medium supplemented with 30 g·L−1 sucrose. Under greenhouse conditions, 98% of micropropagated M. mathildae survived. Plantlets were reintroduced in an experimental plot near to Juriquilla's wild population of M. mathildae; over 52% of the outplanted M. mathildae lot declined after 5 months. Water availability was associated with the decline of outplanted populations during the first month (43%).

scholarly journals CHIA SEED AS A SOURCE OF IN VITRO ESTABLISHMENT OF Salvia hispanica L. PLANTS

2020 ◽  
Vol 51 (3) ◽  
pp. 976-981
Author(s):  
Al- Dabagh & Salih
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Salvia Hispanica ◽  
Chia Seed ◽  
Half Strength ◽  
Salvia Hispanica L ◽  
The Impact

 Technique of tissue culture for Chia (Salvia hispanica) micropropagation was achieved, this study investigated the impact of various concentrations of plant growth regulators on shoot multiplication and root induction with the Chia’s mature seed as a source explant. The highest percentage of shoot formation (80%), shoots number per explant(3.20) and shoot length(3.26 cm), were recorded on MS medium enriched with BAP(1.0 mgl-1) after eight weeks of seed culture. The optimal medium for the rhizogenesis was achieved on half strength MS medium fortified with 1.0 mgl-1 IBA after four weeks of culture, which had the highest rooting percentage (100%) with highest mean of roots number (5.6 roots per shoot) with (3.40 cm root length). The rooted plants were successfully adapted ex vitro with a survival rate of 85%.

scholarly journals Non-symbiotic Seed Germination and In vitro Plant Development of Pholidota articulata

2021 ◽  
Vol 15 ◽  
pp. 44-51
Author(s):  
R. Devendra Prasad ◽  
Shreeti Pradan ◽  
Mukti Ram Poudel ◽  
Bijaya Pant
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Production ◽  
Sustainable Utilization ◽  
Ms Medium ◽  
Natural Habitats ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Symbiotic Seed Germination

Pholidota articulata is an epiphytic orchid mostly used in ornamental cut/pot flower and in traditional medicine. As it has high ornamental and medicinal values, its population from natural habitats is decreasing, therefore, it is listed in the Appendix-II of Convention on International Trade in Endangered Species (CITES). The objective of the present study is to obtain the in vitro plants of P. articulata from seed culture to conserve its germplasm. The in vitro seed germination was carried out in different strengths of Murashige and Skoog (MS) and Knudson C (KnC) medium supplemented with various plant hormones. On the half-strength of MS medium, seeds were started to germinate after 4 weeks of primary culture and they were developed into protocorms with first leaf primordium earlier than on the other medium. Therefore, in vitro developed protocorms were sub-cultured on the half-strength of MS medium supplemented with different concentrations of 6-benzylaminopurine (BAP), gibberellic acid (GA3) and α-naphtalene acetic acid (NAA). They were successfully developed into shoots on the 1.5 mg/l BAP supplemented half-strength of MS medium. Later, they were inoculated on the half-strength of MS medium supplemented with different concentration of α-napthalene acetic acid (NAA), indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) for the root formation, where IBA supplemented medium was found effective for the development of roots. Thus, this study provides a reliable protocol for non-symbiotic seed germination and plant production, and reveals that seed-derived protocorms are good explants for the in vitro mass propagation for conservation and sustainable utilization in horticulture.

scholarly journals Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

HortScience ◽  
2019 ◽  
Vol 54 (9) ◽  
pp. 1558-1564 ◽  
Author(s):  
Georgia Vlachou ◽  
Maria Papafotiou ◽  
Konstantinos F. Bertsouklis
Keyword(s):  
Shoot Multiplication ◽  
Adult Plant ◽  
Naphthaleneacetic Acid ◽  
Similar Response ◽  
Ms Medium ◽  
Rooting Medium ◽  
Shoot Number ◽  
Half Strength ◽  
Mediterranean Plant

Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

scholarly journals Effects of N-Benzyl -9-(2-tetrahydropyranyl and Indole –3-Acetic Acid In Vitro Culture of Bauhinia purpurea L.

2019 ◽  
pp. 238-242
Author(s):  
Belai Meeta Suwal Singh
Keyword(s):  
Analytical Procedure ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Leguminous Plant ◽  
Ms Medium ◽  
Half Strength ◽  
Bauhinia Purpurea ◽  
Mature Seeds ◽  
Indole 3 Acetic Acid

<p>Bauhinia purpurea L. is a leguminous plant moderate sized tree with multipurpose value. It is distributed in sub-Himalayan tracts. It has been cultivated in the plain region up to the elevation of 1350 m. Mature seeds of Bauhinia purpurea L. were cultured on half strength Murashige and Skoog (1962) (MS) medium. Nodal explants obtained from germinated seedlings were cultured on MS medium containing 0.5 M BAP produced multiple shoots which were used for experimental purposes. Nodal explants obtained from cultured were subcultured on different concentrations of N-Benzyl -9-(2-tetrahydropyranyl) (BPA) and Indole-3acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 0.5 M BPA and 0.1 M IAA. After 8 weeks of culture, the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.</p>

scholarly journals Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

2015 ◽  
Vol 23 (1) ◽  
pp. 13-16
Author(s):  
Mafatlal M. Kher ◽  
M. Nataraj ◽  
Hettal D. Parmar ◽  
Hasmatbanu Buchad
Keyword(s):  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Mineral Salts ◽  
Single Node ◽  
The Family ◽  
Sedative Effects ◽  
Bud Breaking ◽  
Important Medicinal Plant

AbstractMerremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

scholarly journals Influence of Additives on Enhanced In vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq.

2013 ◽  
Vol 5 (3) ◽  
pp. 338-345 ◽  
Author(s):  
Swarna JAYAKUMAR ◽  
Ravindhran RAMALINGAM
Keyword(s):  
Large Scale ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Morphological Variations ◽  
Different Types ◽  
Mother Plants ◽  
Half Strength ◽  
Different Parts ◽  
Number Of Shoots

Orthosiphon aristatus is a valuable medicinal plant and different parts of the plant are pharmaceutically used for the treatment of various diseases. The present study was designed to develop an efficient protocol for micropropagation of O. aristatus from nodal explants and to study the influence of additives on the enhancement of the number of shoots per explant. Among the different types of additives used, 10% coconut water and 30 mg/L glutamine added to Murashige and Skoog’s (MS) medium supplemented with 1.0 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L kinetin (KIN) was found to be most effective. Maximum number of shoots (44.07 ± 0.38) with 100% shooting response and shoot length of 7.47 ± 0.10 cm was recorded. In vitro rooting of the microshoots was achieved on half-strength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA), producing an average of 30.27 ± 0.36 roots and 6.02 ± 0.20 cm root length. The rooted shoots were acclimatized with 100% survival rate on coco pith: soil (3:1) planting substrate and was successfully transferred to field conditions. The hardened plants exhibited homogeneity and no morphological variations were observed among the regenerants and the mother plants. Thus, the procedure described is a quick and reliable method which could be applied for efficient large-scale propagation, genetic transformation assays and secondary metabolite production.

Sign in / Sign up

Export Citation Format

Share Document