scholarly journals Nonviral gene delivery of erythropoietin by mesenchymal stromal cells

Gene Therapy ◽  
2011 ◽  
Vol 19 (5) ◽  
pp. 550-560 ◽  
Author(s):  
F Scheibe ◽  
N Gladow ◽  
P Mergenthaler ◽  
A H Tucker ◽  
A Meisel ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Nonviral Gene Delivery

scholarly journals Gene Transfection of Human Turbinate Mesenchymal Stromal Cells Derived from Human Inferior Turbinate Tissues

2016 ◽  
Vol 2016 ◽  
pp. 1-10
Author(s):  
Jin Seon Kwon ◽  
Seung Hun Park ◽  
Ji Hye Baek ◽  
Truong Minh Dung ◽  
Sung Won Kim ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
High Efficiency ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Inferior Turbinate ◽  
Nonviral Gene Delivery ◽  
Charge Ratio ◽  
Pei Nanoparticles

Human turbinate mesenchymal stromal cells (hTMSCs) are novel stem cells derived from nasal inferior turbinate tissues. They are easy to isolate from the donated tissue after turbinectomy or conchotomy. In this study, we applied hTMSCs to a nonviral gene delivery system using polyethyleneimine (PEI) as a gene carrier; furthermore, the cytotoxicity and transfection efficiency of hTMSCs were evaluated to confirm their potential as resources in gene therapy. DNA-PEI nanoparticles (NPs) were generated by adding the PEI solution to DNA and were characterized by a gel electrophoresis and by measuring particle size and surface charge of NPs. The hTMSCs were treated with DNA-PEI NPs for 4 h, and toxicity of NPs to hTMSCs and gene transfection efficiency were monitored using MTT assay, fluorescence images, and flow cytometry after 24 h and 48 h. At a high negative-to-positive charge ratio, DNA-PEI NPs treatment led to cytotoxicity of hTMSCs, but the transfection efficiency of DNA was increased due to the electrostatic effect between the NPs and the membranes of hTMSCs. Importantly, the results of this research verified that PEI could deliver DNA into hTMSCs with high efficiency, suggesting that hTMSCs could be considered as untapped resources for applications in gene therapy.

BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells

2011 ◽  
pp. 485-504 ◽  
Author(s):  
Michael O’Grady ◽  
Robert H. Batchelor ◽  
Kelly Scheyhing ◽  
Christopher W. Kemp ◽  
George T. Hanson ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Multipotent Mesenchymal Stromal Cells

scholarly journals Adenovector-Mediated Gene Delivery to Human Umbilical Cord Mesenchymal Stromal Cells Induces Inner Ear Cell Phenotype

2013 ◽  
Vol 15 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Keerthana Devarajan ◽  
M. Laird Forrest ◽  
Michael S. Detamore ◽  
Hinrich Staecker
Keyword(s):  
Gene Delivery ◽  
Inner Ear ◽  
Umbilical Cord ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Cell Phenotype ◽  
Human Umbilical Cord
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