scholarly journals Growth Hormone-Releasing Peptide 6 Enhances the Healing Process and Improves the Esthetic Outcome of the Wounds

2016 ◽  
Vol 2016 ◽  
pp. 1-11
Author(s):  
Yssel Mendoza Marí ◽  
Maday Fernández Mayola ◽  
Ana Aguilera Barreto ◽  
Ariana García Ojalvo ◽  
Yilian Bermúdez Alvarez ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Universal Model ◽  
Hypertrophic Scars ◽  
Full Thickness ◽  
Rt Pcr ◽  
Effector Cells ◽  
Cutaneous Wounds

In addition to its cytoprotective effects, growth hormone-releasing peptide 6 (GHRP-6) proved to reduce liver fibrotic induration. CD36 as one of the GHRP-6 receptors appears abundantly represented in cutaneous wounds granulation tissue. The healing response in a scenario of CD36 agonistic stimulation had not been previously investigated. Excisional full-thickness wounds (6 mmØ) were created in the dorsum of Wistar rats and topically treated twice a day for 5 days. The universal model of rabbit’s ears hypertrophic scars was implemented and the animals were treated daily for 30 days. Treatments for both species were based on a CMC jelly composition containing GHRP-6 400 μg/mL. Wounds response characterization included closure dynamic, RT-PCR transcriptional profile, histology, and histomorphometric procedures. The rats experiment indicated that GHRP-6 pharmacodynamics involves attenuation of immunoinflammatory mediators, their effector cells, and the reduction of the expression of fibrotic cytokines. Importantly, in the hypertrophic scars rabbit’s model, GHRP-6 intervention dramatically reduced the onset of exuberant scars by activating PPARγ and reducing the expression of fibrogenic cytokines. GHRP-6 showed no effect on the reversion of consolidated lesions. This evidence supports the notion that CD36 is an active and pharmacologically approachable receptor to attenuate wound inflammation and accelerate its closure so as to improve wound esthetic.

scholarly journals Pengaruh Bone Marrow Mesenchymal Stem Cells Terhadap Sekresi VEGF pada Penyembuhan Luka Bakar Tikus

2018 ◽  
Vol 6 (3) ◽  
pp. 702
Author(s):  
Gusti Revilla
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Full Thickness ◽  
Post Test ◽  
Marrow Mesenchymal Stem Cells

Treatment for burns have been carried out in various ways, but have not provided satisfactory results, so that using stem cells is necessary. Administering Bone Marrow Mesenchymal Stem Cells (BM-MSCs) for healing, are by accelerating the formation of granulation tissue, however the effectiveness of the provision of is still under exploration. The objective of this study was to determine the effect of the administration of stem cells BM-MSCs on VEGF secretion on burn healing process in rats. This research was an experimental research with design the post-test only control, using 12 Wistar rats, divided into 2 groups: control (PBS), treatment (BM-MSCs), stem-cells subcutaneously administered dose of 2 x 106 cells / ml. Before that the treated rats anesthetized using xylazine and ketamine then burns are made on the dorsal with full thickness degrees. On day 3 and 7, blood were obtained to measure levels of VEGF was measured by ELISA. The results were analyzed by t-test. Levels of VEGF in day 3 and day 7 in the group given BM-MSC were dereased 12.875 pg/ml dan 23.917 pg/ml compared to the control group 61.667 pg/ml and 200.042 pg/ml and showed significant differences p= 0.012 and p=0.038. The conclusion of this study is the administration of BM-MSCs were the influence levels of VEGF which has into differentiate to cells that contribute to angiogenesis was can to granulation tissue. 

scholarly journals Pengaruh Bone Marrow Mesenchymal Stem Cells Terhadap Sekresi VEGF pada Penyembuhan Luka Bakar Tikus

2018 ◽  
Vol 6 (3) ◽  
pp. 702
Author(s):  
Gusti Revilla
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Control Group ◽  
Full Thickness ◽  
Post Test ◽  
Marrow Mesenchymal Stem Cells

Treatment for burns have been carried out in various ways, but have not provided satisfactory results, so that using stem cells is necessary. Administering Bone Marrow Mesenchymal Stem Cells (BM-MSCs) for healing, are by accelerating the formation of granulation tissue, however the effectiveness of the provision of is still under exploration. The objective of this study was to determine the effect of the administration of stem cells BM-MSCs on VEGF secretion on burn healing process in rats. This research was an experimental research with design the post-test only control, using 12 Wistar rats, divided into 2 groups: control (PBS), treatment (BM-MSCs), stem-cells subcutaneously administered dose of 2 x 106 cells / ml. Before that the treated rats anesthetized using xylazine and ketamine then burns are made on the dorsal with full thickness degrees. On day 3 and 7, blood were obtained to measure levels of VEGF was measured by ELISA. The results were analyzed by t-test. Levels of VEGF in day 3 and day 7 in the group given BM-MSC were dereased 12.875 pg/ml dan 23.917 pg/ml compared to the control group 61.667 pg/ml and 200.042 pg/ml and showed significant differences p= 0.012 and p=0.038. The conclusion of this study is the administration of BM-MSCs were the influence levels of VEGF which has into differentiate to cells that contribute to angiogenesis was can to granulation tissue. 

scholarly journals Efficacy of application of Equisetum pyramidale Goldm. hydrogel for tissue restoration of induced skin lesions in Wistar rats

2020 ◽  
Vol 80 (1) ◽  
pp. 12-22 ◽  
Author(s):  
F. Carmignan ◽  
R. Matias ◽  
C. A. Carollo ◽  
D. M. Dourado ◽  
M. H. Fermiano ◽  
...  
Keyword(s):  
Wistar Rats ◽  
High Efficiency ◽  
Healing Process ◽  
Ethanolic Extract ◽  
Skin Lesions ◽  
Phytochemical Analysis ◽  
Cutaneous Wounds ◽  
Tissue Restoration ◽  
Layer Chromatography ◽  
Flavonoid Patterns

Abstract Medicinal plants, such as E. pyramidale, are alternatives against infections and assist in the healing process of wounds. We evaluated the effects of Hydrogel of E. pyramidale on the healing of cutaneous wounds in animal models by morphological, morphometric and histological analyses, and elucidated major components. The ethanolic extract of E. pyramidale shoots was submitted to phytochemical analysis. For cicatrizing activity assay, Wistar rats were used, and the groups evaluated were hydrogel containing ethanolic extract of E. pyramidale at 2% compared to control groups. The treatment periods were 3, 7 and 21 days, and macroscopic and microscopic analyses were performed. Data were submitted to Analysis of Variance (p <0.05). Phytochemistry and quantification evidenced that flavonoids are main constituents and that they were evidenced by thin layer chromatography (TLC), high efficiency (HPLC) and infrared (IR) methods. In TLC, using flavonoid patterns, we observed pinocembrin. The hydrogel containing ethanolic extract of E. pyramidale at 2% was effective in wound regression. E. pyramidale can be used for the treatment of second intention wounds, and the effective healing may be due to a high flavonoid content.

Healing potential of aStreptomycessp. secondary metabolite, SEM-1-111, on experimental full-thickness excision cutaneous wounds in Wistar rats

2013 ◽  
Vol 38 (2) ◽  
pp. 178-184 ◽  
Author(s):  
S. Sadigh-Eteghad ◽  
A. Dehnad ◽  
J. Mahmodi ◽  
H. Hoseyni ◽  
I. Khalili ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Wistar Rats ◽  
Full Thickness ◽  
Cutaneous Wounds ◽  
Full Thickness Excision

scholarly journals The effect of human adipose-derived multipotent mesenchymal stromal cells in the fibrin gel on the healing of full-thickness skin excision wounds in mice

2017 ◽  
Vol 5 (1) ◽  
pp. 14-22 ◽  
Author(s):  
O. Tykhvynskaya ◽  
O. Rogulska ◽  
N. Volkova ◽  
E. Revenko ◽  
S. Mazur ◽  
...  
Keyword(s):  
Mesenchymal Stromal Cells ◽  
Granulation Tissue ◽  
Stromal Cells ◽  
Healing Process ◽  
Complete Recovery ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Scar Tissue ◽  
Human Blood Plasma ◽  
Full Thickness ◽  
Fibrin Gel

Prospects for the widespread use of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine determine the relevance of studying their abilities to affect the reparative process in experimental systems in vivo.Materials and methods. The effect of human adipose-derived MSCs on the healing rate and completeness of damaged skin site reconstitution was examined using full-thickness excision wound model in mice. The reparative activity of MSCs was revealed in planimetric and histological studies. Human blood plasma-derived fibrin gel was used as a scaffold for MSCs delivery.Results and conclusions. Compared to the spontaneous healing process, application of fibrin gel on the excisional skin wounds promotes earlier maturation of granulation tissue and further formation of loose scar tissue with skin derivates. MSCs in the fibrin gel contribute to the improve of wound epithelialization, the decrease of the inflammatory response, faster maturation of the granulation tissue, including marks of angiogenesis, as well as promotes complete recovery of the dermal and epidermal layers of the damaged site of skin.

scholarly journals Features of engraftment and rejection of ear alatransplants in laboratory rats

2021 ◽  
Vol 18 (4) ◽  
pp. 422-432
Author(s):  
A. A. Astrowski ◽  
H. F. Raduta ◽  
A. A. Bakunovich ◽  
Yu. V. Yarashenka ◽  
A. V. Shuryberka ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Wistar Rats ◽  
Genetic Differences ◽  
Skin Defect ◽  
Full Thickness ◽  
Genetic Affinity ◽  
Laboratory Rats ◽  
Research Institutes ◽  
Thickness Skin ◽  
Immunological Barrier

 A method for revealing genetic affinity between laboratory rats has been developed, which is based on the transplantation of ear skin-chondrocyte allografts onto the surface of a full-thickness skin defect. On the example of Wistar rats (laboratory rats were taken from three research institutes), it was shown that the rejection of allografts occurs in the interval between 8 and 21 days with the same genetic differences that existed between the animals. However, at the same time, only those structures of ear allografts such as the dermis, epidermis and its derivatives die and are rejected, but the layers of chondrocytes are submerged under the granulation tissue and remain in a viable position for at least 210 days. The latter phenomenon is probably due to the membranes that cover the chondrocytes and act as an immunological barrier.

scholarly journals Topical Effect of Pinneaple (Ananas comosus) Juice in Combustio Healing Process Measured by Granulation Process, Reepitelialisation and Angiogenesis

2017 ◽  
Vol 1 (5) ◽  
Author(s):  
Cherry Azaria ◽  
Achadiyani Achadiyani ◽  
Reni Farenia
Keyword(s):  
Treatment Group ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
The Body ◽  
Ananas Comosus ◽  
Histological Structure ◽  
Granulation Process ◽  
Structure Changes ◽  
Experimental Laboratory

Skin protects the body from various trauma, such as high temperature trauma known ascombustio. Pineapple (Ananas comosus) contains lots of substances such as bromelain,antioxidants, and zinc shown to accelerated healing process in previous experiments. Aim ofthis research was to analize the effect of topical pinneaple juice application in combustiohealing process. The method was true experimental laboratory. Wistar rats’ back skin wereinduced by 100 o C iron plat untill 2 nd degree combustio occured, then divided into two groups(n=20). The treatment group were given topical application of pinneaple juice, then the healingprocess were observed macroscopically and microscopically on day 0, 3, 7, and 14. The resultshowed better wound healing in treatment group on day 14. MANOVA test showed topicalapplication of pinneaple juice accelerated healing process (p=0.001). Treatment group showedbetter histological structure changes, as seen from better granulation tissue formed (p=0.003)and reepithelization (p=0,000), but not from angiogenesis (p=0.733). Conclusion, topicalapplication of pinneaple (Ananas comosus) juice accelerated healing process in 2 nd grade ofcombustio animal model measured by granulation maturation process and reepithelizationprocess, but has no effect on angiogenesis.Keywords : Ananas comosus juice, 2 nd degree combustio, granulation tissue, reepitelisation,angiogenesis.

Effect of ethanolic extract of Melia dubia leaves on full‐thickness cutaneous wounds in Wistar rats

2019 ◽  
Vol 32 (6) ◽  
Author(s):  
Ponrasu Thangavel ◽  
Pratima Pathak ◽  
Iyappan Kuttalam ◽  
Suguna Lonchin
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Full Thickness ◽  
Cutaneous Wounds ◽  
Melia Dubia

scholarly journals EFFECT OF SITAGLIPTIN AND VILDAGLIPTIN ON WOUND HEALING IN MALE WISTAR RATS - AN EXPERIMENTAL STUDY

2018 ◽  
Vol 11 (8) ◽  
pp. 392
Author(s):  
Netravathi B Angadi ◽  
Urmila Kagal ◽  
Somaling Timshetti
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Dead Space ◽  
Wistar Rats ◽  
Healing Process ◽  
Dry Weight ◽  
Morbidity And Mortality ◽  
Wound Model ◽  
Male Wistar Rats ◽  
Histopathological Studies

Objective: Diabetes mellitus (DM) is a spectrum of common metabolic disorders, arising from a variety of pathogenic mechanisms. With an increasing incidence worldwide, DM will be likely a leading cause of morbidity and mortality in the future. Delayed wound healing in diabetes is a major source of morbidity and mortality. Sitagliptin and Vildagliptin are novel antihyperglycemic agents used for the treatment of DM. The present study was planned to investigate the effect of Sitagliptin and Vildagliptin on various wound healing models in male Wistar rats.Methods: Male Wistar rats (150–200 g) were divided into three groups, i.e., control, Sitagliptin, and Vildagliptin (n=6 animals in each group) for each wound model. Excision wound, resutured incision wound, and dead space wounds were inflicted under thiopentone anesthesia in male Wistar rats. The rats received Sitagliptin and Vildagliptin orally during the study period. Resutured incision was assessed by wound breaking strength; dead space wound was assessed by granuloma dry weight and histopathology of granulation tissue. In excision wounds, treatment was monitored by planimetry. Data were expressed as mean±standard error of mean and analyzed by analysis of variance followed by Dunnett’s post hoc test. p<0.05 was considered statistically significant.Results: Sitagliptin and Vildagliptin significantly promoted the healing process in all three wound models studied. Histopathological studies revealed increased collagen content and granulation tissue in Sitagliptin and Vildagliptin groups.Conclusion: In all the three wound models studied, Sitagliptin and Vildagliptin promoted wound healing. The pro-healing effect of Sitagliptin and Vildagliptin needs to be explored clinically.

scholarly journals EFFECT OF CHANNA MICROPELTES IN THE GRANULATION, FIBROSIS AND NECROSIS OF DIABETIC WOUND HEALING

2021 ◽  
Vol 6 (2) ◽  
pp. 171
Author(s):  
Amy Nindia Carabelly ◽  
Juliyatin Putri Utami ◽  
Didit Aspriyanto ◽  
Muhammad Hasanu Reksi ◽  
Dewi Puspitasari ◽  
...  
Keyword(s):  
Wound Healing ◽  
Granulation Tissue ◽  
Wistar Rats ◽  
Healing Process ◽  
Wound Complications ◽  
Control Group ◽  
Diabetic Wound ◽  
Model Group ◽  
Delayed Wound Healing ◽  
Diabetic Model

Background: Diabetes mellitus (DM) is characterized by persistent hyperglycemia. The symptoms of DM is delayed wound healing. Delayed wound healing in diabetes will increase the risk of wound complications that will hinder the healing process, and lead to complications such as fibrosis and necrosis. The use of Channa Micropeltes (CM) extract at a dose of 16 mL/kg BW orally is proven to general optimal wound closure and wound contraction clinically in Wistar rats as diabetic model on day 14.However, the histopathological description of wound healing is yet to be known. Objective: To analyze the effect of 16 mL/kg BW dose of CM extract on histopathological changes of wound healing granulation tissue, fibrosis and necrosis in diabetic Wistar rats on day 14. Materials and Methods: This study was a true experimental design with a post-test only control group design. The samples were divided into two groups, namely the diabetic model group given CM extract at 16 mL/kg BW dose for 14 days, and the diabetic model group given BR2 feed only for 14 days. Results: The results of Mann-Whitney U test showed p = 0.003 for granulation tissue, p = 0.411 for fibrosis and p = 1 for necrosis, with the level of significance was p<0.05. Conclusion: The CM extract at 16 mL/kg BW dose affects the histopathological description of granulation tissue which presents a better result than those in the control group but did not affect the features of fibrosis and necrosis in diabetic Wistar rats on day 14. Keywords: Channa Micropeltes, Diabetic wound, Fibrosis, Granulation tissue, Necrosis.

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