scholarly journals Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Zeta Potential ◽  
Polymethyl Methacrylate ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Contact Angles ◽  
Equilibrium Analysis ◽  
Value Analysis ◽  
Biofilm Development

It is postulated that biofilm formation in the oral cavity causes some oral diseases. Lactoferrin is an antibacterial protein in saliva and an important defense factor against biofilm development. We analyzed the adsorbed amount of lactoferrin and the dissociation constant (Kd) of lactoferrin to the surface of different dental materials using an equilibrium analysis technique in a 27 MHz quartz crystal microbalance (QCM) measurement. Four different materials, titanium (Ti), stainless steel (SUS), zirconia (ZrO2) and polymethyl methacrylate (PMMA), were evaluated. These materials were coated onto QCM sensors and the surfaces characterized by atomic force microscopic observation, measurements of surface roughness, contact angles of water, and zeta potential. QCM measurements revealed that Ti and SUS showed a greater amount of lactoferrin adsorption than ZrO2and PMMA. Surface roughness and zeta potential influenced the lactoferrin adsorption. On the contrary, theKdvalue analysis indicated that the adsorbed lactoferrin bound less tightly to the Ti and SUS surfaces than to the ZrO2and PMMA surfaces. The hydrophobic interaction between lactoferrin and ZrO2and PMMA is presumed to participate in better binding of lactoferrin to ZrO2and PMMA surfaces. It was revealed that lactoferrin adsorption behavior was influenced by the characteristics of the material surface.

Study of Asphaltene Deposition onto Stainless-Steel Surfaces Using Quartz Crystal Microbalance with Dissipation

2020 ◽  
Vol 34 (8) ◽  
pp. 9283-9295 ◽  
Author(s):  
Fang Liu ◽  
Scott Hickman ◽  
Tabish Maqbool ◽  
Vincent Pauchard ◽  
Sanjoy Banerjee
Keyword(s):  
Stainless Steel ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Asphaltene Deposition ◽  
Steel Surfaces

scholarly journals Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
You Kusakawa ◽  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Adsorption Rate ◽  
Force Microscopy ◽  
Atomic Force ◽  
A Cell ◽  
Addition Amount

Protein adsorption onto titanium (Ti) or zirconia (ZrO2) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO2 sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO2 sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO2 sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO2 sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO2 may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO2, Alb adsorption rate to Ti was faster than that to ZrO2. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate in vivo Fn and Alb adsorption to Ti or ZrO2.

Modification of Polystyrene Morphology and its Influence to the Coated Zinc Phthalocyanine Layer and Frequency Change in QCM Sensor

2015 ◽  
Vol 827 ◽  
pp. 257-261 ◽  
Author(s):  
Masruroh ◽  
D.J.D.H. Djoko ◽  
Lalu A. Didik ◽  
Eka Rachmawati ◽  
Fadli Robiandi ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Quartz Crystal Microbalance ◽  
Bovine Serum ◽  
Quartz Crystal ◽  
Zinc Phthalocyanine ◽  
Frequency Change ◽  
Polystyrene Surface ◽  
Influence Of Solvents

This study observed the influence of solvents on polystyrene regarding the layered morphologies of zinc phthalocyanine (ZnPc) and its influence on the bovine serum albumin (BSA) immobilization on quartz crystal microbalance (QCM) sensor. The larger surface width introduced by surface roughness of the polystyrene due to chloroform (CHCl3) as the solvents allows more ZnPc to be trapped within the polystyrene. Moreover, the wider surface width also increases the amount of ZnPc to be deposited on top of the polystyrene surface. Therefore, the ZnPc layer deposited onto polystyrene created with CHCl3 solvent is thicker than other solvents, which result in the largest ∆f of the ZnPc layer.

scholarly journals Surface Roughness and Streptococcus mutans Adhesion on Metallic and Ceramic Fixed Prosthodontic Materials after Scaling

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 1027
Author(s):  
Jenni Hjerppe ◽  
Sampo Rodas ◽  
Johanna Korvala ◽  
Paula Pesonen ◽  
Anna Kaisanlahti ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Surface Treatment ◽  
Streptococcus Mutans ◽  
Bacterial Adhesion ◽  
Lithium Disilicate ◽  
Human Saliva ◽  
Contact Angles ◽  
Cobalt Chromium ◽  
Ultrasonic Scaler

The aim of this study was to evaluate the surface roughness of fixed prosthodontic materials after polishing or roughening with a stainless steel curette or ultrasonic scaler and to examine the effect of these on Streptococcus mutans adhesion and biofilm accumulation. Thirty specimens (10 × 10 × 3 mm3) of zirconia (Zr), pressed lithium disilicate (LDS-Press), milled lithium disilicate glazed (LDS-Glaze), titanium grade V (Ti) and cobalt-chromium (CoCr) were divided into three groups (n = 10) according to surface treatment: polished (C), roughened with stainless steel curette (SC), roughened with ultrasonic scaler (US). Surface roughness values (Sa, Sq) were measured with a spinning disc confocal microscope, and contact angles and surface free energy (SFE) were measured with a contact angle meter. The specimens were covered with sterilized human saliva and immersed into Streptococcus mutans suspensions for bacterial adhesion. The biofilm was allowed to form for 24 h. Sa values were in the range of 0.008–0.139 µm depending on the material and surface treatment. Curette and ultrasonic scaling increased the surface roughness in LDS-Glaze (p < 0.05), Ti (p < 0.01) and CoCr (p < 0.001), however, surface roughness did not affect bacterial adhesion. Zr C and US had a higher bacterial adhesion percentage compared to LDS-Glaze C and US (p = 0.03). There were no differences between study materials in terms of biofilm accumulation.

Adsorption behaviors of salivary pellicle proteins onto denture base metals using 27-MHz quartz crystal microbalance

2020 ◽  
pp. 1-11
Author(s):  
Masatsugu Hirota ◽  
Tohru Hayakawa
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Contact Angles ◽  
Salivary Proteins ◽  
Material Surface ◽  
Chromium Alloy ◽  
Base Metals ◽  
Denture Base ◽  
Salivary Pellicle ◽  
The Difference

BACKGROUND: The adsorption of salivary pellicle proteins onto the material surface is key for denture plaque formation. OBJECTIVE: We aimed to investigate the adsorption of bovine serum albumin (BSA) and mucin (MCN) onto denture base metal materials using a 27-MHz quartz crystal microbalance (QCM) method. METHODS: A gold (Au), titanium (Ti), and cobalt chromium alloy (Co–Cr) sensors were employed for QCM measurements. Adsorbed amounts of BSA or MCN were calculated by observing the frequency decrease, and the apparent reaction rate, kobs, was obtained by the curve fitting of the frequency shift against the adsorption time. RESULTS: The adsorbed amounts of BSA on Ti were significantly lower than those on Au and Co–Cr. For MCN adsorption, Au showed significantly greater amounts of adsorption than Co–Cr. The kobs of Ti for BSA adsorption was significantly smaller than for the Co–Cr. The kobs of Ti, and Co–Cr for MCN adsorption were significantly smaller than for the Au. A clear correlation was not determined between adsorbed amounts of BSA or MCN onto each sensor and the surface topography or contact angles. CONCLUSIONS: The difference of denture base metals and the difference of salivary proteins influences the adsorption behavior of salivary proteins.

Quartz Crystal Microbalance for the Transient Study of Electrode Processes

1995 ◽  
Vol 411 ◽  
Author(s):  
V. Daujotis ◽  
R Raudonis ◽  
V. Kubilius ◽  
E. Gaidamauskas ◽  
R. Sližys
Keyword(s):  
Surface Roughness ◽  
Quartz Crystal Microbalance ◽  
Oscillation Frequency ◽  
Quartz Crystal ◽  
Solution Viscosity ◽  
Electrode Processes ◽  
Initial Deviation ◽  
Frequency Responses ◽  
Faradaic Reaction ◽  
Transient Study

ABSTRACTA technique for the measurement of quartz supported electrode oscillation frequency with special reference to transient electrochemical experiments was prepared. Due to changes in solution viscosity and density and in surface roughness during potential step experiments, the error in calculation of mass changes from frequency responses can reach 3–5 %. Initial deviation of electron numbers from unity by 30–60% during pulse electroreduction of dicyanoargentate shows that this process is not a simple faradaic reaction.

scholarly journals Use of a Quartz Crystal Microbalance To Investigate the Antiadhesive Potential of N-Acetyl-l-Cysteine

2005 ◽  
Vol 71 (5) ◽  
pp. 2705-2712 ◽  
Author(s):  
Ann-Cathrin Olofsson ◽  
Malte Hermansson ◽  
Hans Elwing
Keyword(s):  
Stainless Steel ◽  
Life Cycle ◽  
Cell Surface ◽  
Quartz Crystal Microbalance ◽  
Viscoelastic Properties ◽  
Quartz Crystal ◽  
Life Cycle Stage ◽  
Bacterial Biofilm ◽  
Initial Adhesion ◽  
Steel Surfaces

ABSTRACT The reduction of bacterial biofilm formation on stainless steel surfaces by N-acetyl-l-cysteine (NAC) is attributed to effects on bacterial growth and polysaccharide production, as well as an increase in the wettability of steel surfaces. In this report, we show that NAC-coated stainless steel and polystyrene surfaces affect both the initial adhesion of Bacillus cereus and Bacillus subtilis and the viscoelastic properties of the interaction between the adhered bacteria and the surface. A quartz crystal microbalance with dissipation was shown to be a powerful and sensitive technique for investigating changes in the applied NAC coating for initial cell surface interactions of bacteria. The kinetics of frequency and dissipation shifts were dependent on the bacteria, the life cycle stage of the bacteria, and the surface. We found that exponentially grown cells gave rise to a positive frequency shift as long as their cell surface hydrophobicity was zero. Furthermore, when the characteristics of binding between the cell and the surface for different growth phases were compared, the rigidity increased from exponentially grown cells to starved cells. There was a trend in which an increase in the viscoelastic properties of the interaction, caused by the NAC coating on stainless steel, resulted in a reduction in irreversibly adhered cells. Interestingly, for B. cereus that adhered to polystyrene, the viscoelastic properties decreased, while there was a reduction in adhered cells, regardless of the life cycle stage. Altogether, NAC coating on surfaces was often effective and could both decrease the initial adhesion and increase the detachment of adhered cells and spores. The most effective reduction was found for B. cereus spores, for which the decrease was caused by a combination of these two parameters.

scholarly journals Challenges associated with Quartz Crystal Microbalance with Dissipation (QCM-D) as a wettability screening tool

2018 ◽  
Vol 73 ◽  
pp. 58 ◽  
Author(s):  
Samuel Erzuah ◽  
Ingebret Fjelde ◽  
Aruoture Voke Omekeh
Keyword(s):  
Stainless Steel ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Flow Cell ◽  
Reservoir Rock ◽  
Salt Precipitation ◽  
Frequency Signal ◽  
Metallic Component ◽  
High Attenuation ◽  
Set Up

Wettability is an indispensable parameter in multiphase flow due to its profound effect in fluid phase distribution and flow properties in the oil reservoirs. One approach of unravelling the enigma associated with wettability characterization is to investigate oil adhesion onto reservoir rock surface during crude oil accumulation. This was accomplished using Quartz Crystal Microbalance with Dissipation (QCM-D) device. The QCM-D is a microbalance device that hinges on the changes in the frequency of a resonating crystal due to changes in the mass on sensor surface, precipitation, adsorption and desorption. However, this technique was confronted with numerous challenges during its early try-out. The objective of this study is to enumerate these challenges and how they were resolved. The piston-cell, valves, flow-lines and most of the experimental set-up were made from stainless steel. Hence, the high temperature coupled with high salinity brine resulted in the formation and deposition of corroded materials on the sensor. Due to the high sensitivity of the QCM-D technique, these corrosion deposits were detected via the high attenuation of the frequency signal as time elapsed during Formation Water (FW) injection. The second challenge was related to the dissolution of the thin sensor coatings (sensor etching) depicted by the relatively high increase in frequency signal with negligible changes in Dissipation (D). The third challenge was related to the trapping of fluids such as Stock Tank Oil (STO) inside the flow-cell. Finally, salt precipitation resulting from temperature variation during the initial experimental set-up was also observed. To resolve the corrosion challenge, all the stainless-steel components in the experimental set-up were replaced with titanium and non-metallic component such as peek materials. The sensor etching was also averted by injecting the brine through a packed column filled with similar mineral as the coatings on the sensor to attain equilibrium prior to injecting it onto the sensor. Geochemical simulation of the sensor etching was also confirmed using the geochemical simulator PHREEQ-C. Furthermore, the trapping of fluids inside the flow-cell was overcome by rotating the flow-cell to optimize the fluid displacement via capitalizing on their density contrast. Finally, the salt precipitation was avoided by conducting the experiment in a constant temperature experimental set-up. The QCM-D technique can be employed to estimate wettability by evaluating the tendency of the various minerals to adhere oil. The beauty of the QCM-D technique is that the surface interactions can be monitored on a real-time.

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