scholarly journals Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
You Kusakawa ◽  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Protein Adsorption ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Adsorption Rate ◽  
Force Microscopy ◽  
Atomic Force ◽  
A Cell ◽  
Addition Amount

Protein adsorption onto titanium (Ti) or zirconia (ZrO2) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO2 sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO2 sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO2 sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO2 sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO2 may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO2, Alb adsorption rate to Ti was faster than that to ZrO2. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate in vivo Fn and Alb adsorption to Ti or ZrO2.

Charge-Dependent Sidedness of Cytochrome P450 Forms Studied by Quartz Crystal Microbalance and Atomic Force Microscopy

2001 ◽  
Vol 385 (1) ◽  
pp. 78-87 ◽  
Author(s):  
John B. Schenkman ◽  
Ingela Jansson ◽  
Yuri Lvov ◽  
James F. Rusling ◽  
Salah Boussaad ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Cytochrome P450 ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Force Microscopy ◽  
Atomic Force

Assessment of DNA Binding to Human Rad51 Protein by using Quartz Crystal Microbalance and Atomic Force Microscopy: Effects of ADP and BRC4-28 Peptide Inhibitor

ChemPhysChem ◽  
2014 ◽  
Vol 15 (17) ◽  
pp. 3753-3760 ◽  
Author(s):  
Charles Esnault ◽  
Axelle Renodon-Cornière ◽  
Masayuki Takahashi ◽  
Nathalie Casse ◽  
Nicolas Delorme ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Dna Binding ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Peptide Inhibitor ◽  
Rad51 Protein ◽  
Force Microscopy ◽  
Atomic Force ◽  
Human Rad51

Probing molecular interactions between humic acid and surface-grafted polyacrylamide using quartz crystal microbalance with dissipation and atomic force microscopy: implications for environmental remediation

2018 ◽  
Vol 15 (6) ◽  
pp. 336 ◽  
Author(s):  
Omar Maan ◽  
Jun Huang ◽  
Hongbo Zeng ◽  
Qingye Lu
Keyword(s):  
Atomic Force Microscopy ◽  
Humic Acid ◽  
Quartz Crystal Microbalance ◽  
Molecular Interactions ◽  
Environmental Remediation ◽  
Quartz Crystal ◽  
Major Constituent ◽  
Environmental Behaviour ◽  
Force Microscopy ◽  
Atomic Force

Environmental contextPolyacrylamide and its derivatives may enter the natural environment as a consequence of their wide use in various industrial applications. This study demonstrates the application of a quartz crystal microbalance and atomic force microscopy to study the molecular interactions between polyacrylamides and humic acids under various solution chemistries. The knowledge obtained can be used to understand and predict the environmental behaviour of polyacrylamides. AbstractA fundamental understanding of the environmental behaviour of polyacrylamide (PAM) is of importance for guiding environmental remediation. We create a framework for understanding the molecular interactions between PAM and a major constituent present in all natural waters and soil, humic acid (HA), using a quartz crystal microbalance with dissipation (QCM-D) and an atomic force microscope (AFM). A thin film of PAM was grafted on a silica surface silanised with 3-(trimethoxysilyl)propyl methacrylate and the resulting surface was characterised by X-ray photoelectron spectroscopy for the chemical bonds and composition, secondary ion mass spectrometry for the composition and molecular weight, water contact angle measurements for the hydrophilicity, AFM for the morphology, and ellipsometry for the thickness. Surface-grafted PAM was used to study its interactions with HA in aqueous solutions at different pH (2, 7, and 10) and NaCl salt concentrations (1, 10, and 100 mM, within the range of salt concentrations of fresh water) using QCM-D. QCM-D measurements showed that compared with bare silica, the adsorption of HA by PAM-coated silica was greatly reduced at all pHs and salt concentrations, and the adsorption of HA on PAM-coated silica depended on the solution chemistry including solution pH and salt concentration. Hydrogen bonding between PAM and HA is the major driving force for HA to adsorb on PAM. AFM force measurements showed that adhesion between PAM and HA was observed only at acidic conditions. The knowledge obtained from this study will benefit the prediction of the environmental behaviour of PAMs under different conditions in natural/engineered environments and provide guidance for the design of remediation technologies for water and soil.

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