Adsorption behaviors of salivary pellicle proteins onto denture base metals using 27-MHz quartz crystal microbalance

2020 ◽  
pp. 1-11
Author(s):  
Masatsugu Hirota ◽  
Tohru Hayakawa
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Contact Angles ◽  
Salivary Proteins ◽  
Material Surface ◽  
Chromium Alloy ◽  
Base Metals ◽  
Denture Base ◽  
Salivary Pellicle ◽  
The Difference

BACKGROUND: The adsorption of salivary pellicle proteins onto the material surface is key for denture plaque formation. OBJECTIVE: We aimed to investigate the adsorption of bovine serum albumin (BSA) and mucin (MCN) onto denture base metal materials using a 27-MHz quartz crystal microbalance (QCM) method. METHODS: A gold (Au), titanium (Ti), and cobalt chromium alloy (Co–Cr) sensors were employed for QCM measurements. Adsorbed amounts of BSA or MCN were calculated by observing the frequency decrease, and the apparent reaction rate, kobs, was obtained by the curve fitting of the frequency shift against the adsorption time. RESULTS: The adsorbed amounts of BSA on Ti were significantly lower than those on Au and Co–Cr. For MCN adsorption, Au showed significantly greater amounts of adsorption than Co–Cr. The kobs of Ti for BSA adsorption was significantly smaller than for the Co–Cr. The kobs of Ti, and Co–Cr for MCN adsorption were significantly smaller than for the Au. A clear correlation was not determined between adsorbed amounts of BSA or MCN onto each sensor and the surface topography or contact angles. CONCLUSIONS: The difference of denture base metals and the difference of salivary proteins influences the adsorption behavior of salivary proteins.

scholarly journals Adsorption Analysis of Lactoferrin to Titanium, Stainless Steel, Zirconia, and Polymethyl Methacrylate Using the Quartz Crystal Microbalance Method

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Eiji Yoshida ◽  
Tohru Hayakawa
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Zeta Potential ◽  
Polymethyl Methacrylate ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Contact Angles ◽  
Equilibrium Analysis ◽  
Value Analysis ◽  
Biofilm Development

It is postulated that biofilm formation in the oral cavity causes some oral diseases. Lactoferrin is an antibacterial protein in saliva and an important defense factor against biofilm development. We analyzed the adsorbed amount of lactoferrin and the dissociation constant (Kd) of lactoferrin to the surface of different dental materials using an equilibrium analysis technique in a 27 MHz quartz crystal microbalance (QCM) measurement. Four different materials, titanium (Ti), stainless steel (SUS), zirconia (ZrO2) and polymethyl methacrylate (PMMA), were evaluated. These materials were coated onto QCM sensors and the surfaces characterized by atomic force microscopic observation, measurements of surface roughness, contact angles of water, and zeta potential. QCM measurements revealed that Ti and SUS showed a greater amount of lactoferrin adsorption than ZrO2and PMMA. Surface roughness and zeta potential influenced the lactoferrin adsorption. On the contrary, theKdvalue analysis indicated that the adsorbed lactoferrin bound less tightly to the Ti and SUS surfaces than to the ZrO2and PMMA surfaces. The hydrophobic interaction between lactoferrin and ZrO2and PMMA is presumed to participate in better binding of lactoferrin to ZrO2and PMMA surfaces. It was revealed that lactoferrin adsorption behavior was influenced by the characteristics of the material surface.

scholarly journals Analysis of the Effect on Denture Base Metal of Cleaning with Denture Cleanser Using the Quartz Crystal Microbalance Method

Hygiene ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 129-139
Author(s):  
Naoya Imaizumi ◽  
Toshitsugu Sakurai ◽  
Masatsugu Hirota ◽  
Tohru Hayakawa ◽  
Chikahiro Ohkubo
Keyword(s):  
Base Metal ◽  
Removal Rate ◽  
Salivary Proteins ◽  
Base Metals ◽  
Denture Base ◽  
Cleaning Agents ◽  
Significant Difference ◽  
Plaque Control ◽  
Basic Studies ◽  
Denture Cleansers

Denture plaque control for the prevention of aspiration pneumonia is very important. The pellicle is the major cause of denture plaque adhesion. Few basic studies have evaluated the effectiveness of denture cleansers for pellicles composed of salivary proteins. The adhesion of salivary proteins formed on denture base metal and the removal rate were quantitatively analyzed using the QCM method after denture cleanser injection. This is the first study to compare the cleaning effects of denture cleanser on denture base metal using the QCM method. Au and Ti sensors were employed as the denture base metals. Albumin was used for the adsorption of salivary proteins. The results showed that no significant difference was found between Au and Ti in the amounts of albumin adsorbed, and the rate of albumin removal from Ti was significantly higher than that of Au. In this study, the cleaning effectiveness of denture cleanser was confirmed based on the adsorbed amount and the removal rate of salivary proteins adsorbed onto denture base metals. Thus, the QCM method was suggested to be a useful tool for removing the effects of salivary proteins from denture cleaning agents on denture base metal.

Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal

Effect of Current Distribution on Quartz Crystal Microbalance Measurements

1998 ◽  
Vol 145 (2) ◽  
pp. 492-497 ◽  
Author(s):  
James J. Kelly ◽  
K. M. Anisur Rahman ◽  
Christopher J. Durning ◽  
Alan C. West
Keyword(s):  
Quartz Crystal Microbalance ◽  
Current Distribution ◽  
Quartz Crystal
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