scholarly journals In VitroCharacterization of Human Mesenchymal Stem Cells Isolated from Different Tissues with a Potential to Promote Complex Bone Regeneration

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Áron Szepesi ◽  
Zsolt Matula ◽  
Anna Szigeti ◽  
György Várady ◽  
József Szalma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Periodontal Ligament ◽  
Human Mesenchymal Stem Cells ◽  
Bone Grafts ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential ◽  
Medical Need

Bone tissue regeneration is a major, worldwide medical need, and several strategies have been developed to support the regeneration of extensive bone defects, including stem cell based bone grafts. In addition to the application of stem cells with high osteogenic potential, it is important to maintain proper blood flow in a bone graft to avoid inner graft necrosis. Mesenchymal stem cells (MSCs) may form both osteocytes and endothelial cells; therefore we examined the combinedin vitroosteogenic and endothelial differentiation capacities of MSCs derived from adipose tissue, Wharton’s jelly, and periodontal ligament. Based on a detailed characterization presented here, MSCs isolated from adipose tissue and periodontal ligament may be most appropriate for generating vascularized bone grafts.

scholarly journals In Vitro Osteogenic Potential of Human Mesenchymal Stem Cells Is Predicted by Runx2/Sox9 Ratio

2015 ◽  
Vol 21 (1-2) ◽  
pp. 115-123 ◽  
Author(s):  
Claudia Loebel ◽  
Ewa M. Czekanska ◽  
Marco Bruderer ◽  
Gian Salzmann ◽  
Mauro Alini ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential

scholarly journals The subpopulation of CD105 negative mesenchymal stem cells show strong immunomodulation capacity compared to CD105 positive mesenchymal stem cells

2019 ◽  
Vol 6 (4) ◽  
pp. 3131-3140 ◽  
Author(s):  
Liem Hieu Pham ◽  
Ngoc Bich Vu ◽  
Phuc Van Pham
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Umbilical Cord ◽  
Immune Modulation ◽  
Human Mesenchymal Stem Cells ◽  
Human Adipose Tissue ◽  
Human Umbilical Cord ◽  
Differentiation Potential

Introduction: Human mesenchymal stem cells (MSCs) are the most popular stem cells applied in disease treatment. MSCs can be isolated and in vitro expanded from various sources such as bone marrow, peripheral blood, umbilical cord blood, umbilical cord tissue, and adipose tissue. According to Dominici et al. (2006), MSCs should express CD105, an essential marker used to confirm MSCs. However, some recent studies have show that MSCs contained a subpopulation that is negative for CD105. This study aimed to compare the immune modulation capacity of 2 populations of CD105 positive (CD105+) and negative (CD105-) MSCs derived from 2 sources: human adipose tissue (AT) and human umbilical cord (UC). Methods: MSCs were isolated from human adipose tissues (adipose tissue-derived mesenchymal stem cells – AT-MSCs) and human umbilical cord (umbilical cord-derived mesenchymal stem cells – UC-MSCs) according to previously published protocols. The two populations of CD105- and CD105+ MSCs were sorted based on the expression of CD105 from AT-MSCs and UC-MSCs. Four populations of CD105 (AT-MSCs, CD105+ AT-MSCs, CD105- UC-MSCs, and CD105+ UC-MSCs) were used to compare the phenotype as well as in vitro differentiation potential; then they were used to evaluate the immune modulation capacity by allogeneic T cell suppression and cytokine release. Results: The results showed that CD105- MSCs from AT and UC exhibited an immune modulation capacity that was much stronger than CD105+ MSCs from the same source of AT and UC. The strong immunomodulation of CD105- MSCs may relate to autocrine production of TGF-beta 1 by MSCs. Conclusion: The results suggested that CD105- MSCs are promising MSCs for application in regenerative medicine, especially for the treatment of diseases related to inflammation.  

scholarly journals Epithelial in vitro differentiation of human mesenchymal stem cells (hMSCs) from adipose tissue (AT) and bone marrow (BM): cellular characterization and study of HLA I and II expression

2016 ◽  
Vol 101 (798) ◽  
pp. 72-78
Author(s):  
Álvaro Sierra-Sánchez ◽  
Antonio Ruiz-García ◽  
Jorge Guerrero Calvo ◽  
Natividad Fernández Porcel ◽  
Olga Espinosa Ibáñez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Differentiation

scholarly journals A New Approach for Adipose Tissue Regeneration Based on Human Mesenchymal Stem Cells in Contact to Hydrogels-an In Vitro Study

2009 ◽  
Vol 11 (10) ◽  
pp. B155-B161 ◽  
Author(s):  
Kirsten Peters ◽  
Achim Salamon ◽  
Sandra Van Vlierberghe ◽  
Joachim Rychly ◽  
Michael Kreutzer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Tissue Regeneration ◽  
Human Mesenchymal Stem Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
New Approach

Particle Size of Hydroxyapatite Granules Calcified from Red Algae Affects the Osteogenic Potential of Human Mesenchymal Stem Cells in vitro

2006 ◽  
Vol 182 (2) ◽  
pp. 79-88 ◽  
Author(s):  
Martina Weissenböck ◽  
Elisabeth Stein ◽  
Gerhard Undt ◽  
Rolf Ewers ◽  
Günter Lauer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Particle Size ◽  
Mesenchymal Stem Cells ◽  
Red Algae ◽  
Human Mesenchymal Stem Cells ◽  
Osteogenic Potential
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