Osteogenic potential of stem cells-seeded bioactive nanocomposite scaffolds: A comparative study between human mesenchymal stem cells derived from bone, umbilical cord Wharton's jelly, and adipose tissue

2016 ◽  
Vol 106 (1) ◽  
pp. 61-72 ◽  
Author(s):  
Saeid Kargozar ◽  
Masoud Mozafari ◽  
Seyed Jafar Hashemian ◽  
Peiman Brouki Milan ◽  
Sepideh Hamzehlou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Comparative Study ◽  
Umbilical Cord ◽  
Human Mesenchymal Stem Cells ◽  
Wharton’S Jelly ◽  
Osteogenic Potential ◽  
Wharton's Jelly ◽  
Nanocomposite Scaffolds

scholarly journals Osteogenic differentiation of human mesenchymal stem cells from adipose tissue and Wharton’s jelly of the umbilical cord

2017 ◽  
Vol 64 (2) ◽  
Author(s):  
Alicja Zajdel ◽  
Magdalena Kałucka ◽  
Edyta Kokoszka-Mikołaj ◽  
Adam Wilczok
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Osteogenic Differentiation ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Calcium Deposition ◽  
Human Umbilical Cord ◽  
Wharton's Jelly ◽  
Alp Activity

Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injures treatment. Human umbilical cord and adipose tissue are routinely discarded as clinical waste and may be used as uncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration.The aim of this research was to investigate the osteogenic potential of human MSCs derived from adipose tissue (ASCs) and Wharton’s jelly of the human umbilical cord (WJ-MSCs) differentiated under the same conditions.Osteogenic differentiation of MSCs was detected and quantified by ARS staining for calcium deposition and alkaline phosphatase (ALP) activity, osteoprotegerin (OPG), and osteocalcin (OC) secretion measurements. Under osteogenic conditions the measured ALP activity and calcium deposition were significantly higher in ASCs than in WJ-MSCs, while the OPG and OC secretion were higher in WJ-MSCs vs. ASCs. Low concentrations of OPG and high levels of OC in ASCs and WJ-MSCs, prove that these cells reached an advanced stage of the osteogenic differentiation. The levels of OC secreted by ASCs were lower than by WJ-MSCs what indicates that the differentiation process of the ASCs reached the stage when the extracellular matrix is overproduced and the down-regulation of OC begins.Both cell types, ASCs and WJ-MSCs possess potential to differentiate towards the osteogenic lineage. However, the observed differences in the levels of osteogenic markers suggest that ASCs may be better candidates for cell-based osteogenesis than WJ-MSCs.

scholarly journals Exosomes derived from human umbilical cord Wharton's jelly mesenchymal stem cells ameliorate experimental lymphedema

2021 ◽  
Vol 11 (7) ◽  
Author(s):  
Zhao Ting ◽  
Yan Zhi‐xin ◽  
Tan You‐wen ◽  
Yang Fu‐ji ◽  
Sun Hui ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

scholarly journals Enhanced Chondrogenic Differentiation of Human Umbilical Cord Wharton's Jelly Derived Mesenchymal Stem Cells by GSK-3 Inhibitors

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0168059 ◽  
Author(s):  
Prapot Tanthaisong ◽  
Sumeth Imsoonthornruksa ◽  
Apichart Ngernsoungnern ◽  
Piyada Ngernsoungnern ◽  
Mariena Ketudat-Cairns ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Chondrogenic Differentiation ◽  
Wharton’S Jelly ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

382 ISOLATION AND CHARACTERIZATION OF SIZE-SIEVED MESENCHYMAL STEM CELLS FROM PERIVASCULAR AND INTERVASCULAR WHARTON'S JELLY OF HORSE UMBILICAL CORD

2010 ◽  
Vol 22 (1) ◽  
pp. 347 ◽  
Author(s):  
A. Corradetti ◽  
A. Lange Consiglio ◽  
M. Barucca ◽  
F. Cremonesi ◽  
D. Bizzaro
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Clinical Importance ◽  
Wharton’S Jelly ◽  
Small Cells ◽  
Time Range ◽  
Homogeneous Population ◽  
Wharton's Jelly ◽  
Isolation And Characterization

Horse umbilical cord has recently been suggested as a potential source for mesenchymal stem cells (MSC). Despite their clinical importance for treating injuries to musculoskeletal tissues, there is still not a well-defined protocol for the isolation and expansion of MSC in culture. Literature shows few experiments conducted on equine MSC; in these experiments cells are isolated primarily by their tight adherence to culture plastic dishes. These cells are initially heterogeneous so we aimed to separate homogeneous subpopulations of MSC using multi-dishes with transwell inserts of 8 μm pores. After digestion of perivascular and intervascular Wharton’s jelly with collagenase (0.75 mg mL-1) for 16 h at 37°C, 12 primary cell cultures from 3 animals were obtained. Cells were plated at a density of 106 cells/cm2 on these culture dishes. The lower (8μm) and upper populations of perivascular and intervascular cells, cultured in fetal bovine serum-supplemented DMEM-HG, with EGF, were studied for their morphology, renewal capacity, mesenchymal markers expression, and differentiating potential. Cells with less than an 8 μm diameter that adhered to the lower plate surface were a morphological homogeneous population if compared with the upper larger sized population of either perivascular or intervascular jelly. Every subpopulation steadily proliferated over the passages studied, without spontaneous differentiation, reaching confluence even after 10 passages. The large cells of the perivascular portion propagated slowly and passed 16.58 cell population doublings (PD) after 31 days, whereas in the same time range, the small cells reached 19.49 cell PD. After the seventh passage, the proliferating traits of the 2 cell populations became similar. As a control, the unsieved perivascular portion passed 8.54 cell PD. On the contrary, in the intervascular portion, the large cells propagated more rapidly with respect to the small ones (20.53 v. 13.66 cell PD) and the unsieved control (9.42 cell PD). The fibroblast colony forming unit (CFU-F) assay supported these differences with greater CFU-F for the small perivascular cells and the large intervascular cells (the rates were, respectively, 1:133 and 1:106). As shown by RT-PCR, every subpopulation was positive for MSC markers (CD105, CD 44, CD 29) and CD34 negative. Osteogenic differentiation of each subpopulation was confirmed by von Kossa stain and osteocalcin mRNA expression after 10 days of induction. At this time point, only intervascular cells expressed osteopontin, suggesting an earlier expression of this marker in these cells. We suggest that in the perivascular portion, the large cells are mature MSC and the smaller ones are recycling stem cells and that in the intervascular fraction, MSC do not divide rapidly until after they are separated from non-MSC of whole primary culture. The size-sieving procedure is a simple and effective method to isolate more proliferative MSC.

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