scholarly journals Retracted: Flavonoid-Deficient Mutants in Grass Pea (Lathyrus sativusL.): Genetic Control, Linkage Relationships, and Mapping with Aconitase and S-Nitrosoglutathione Reductase Isozyme Loci

2016 ◽  
Vol 2016 ◽  
pp. 1-1
2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Dibyendu Talukdar

Two flavonoid-deficient mutants, designated asfldL-1andfldL-2, were isolated in EMS-mutagenized (0.15%, 10 h) M2progeny of grass pea (Lathyrus sativusL.). Both the mutants contained total leaf flavonoid content only 20% of their mother varieties. Genetic analysis revealed monogenic recessive inheritance of the trait, controlled by two different nonallelic loci. The two mutants differed significantly in banding patterns of leaf aconitase (ACO) and S-nitrosoglutathione reductase (GSNOR) isozymes, possessing unique bands inAco 1, Aco 2,andGsnor 2loci. Isozyme loci inherited monogenically showing codominant expression in F2(1 : 2 : 1) and backcross (1 : 1) segregations. Linkage studies and primary trisomic analysis mappedAco 1andfld 1loci on extra chromosome of trisomic-I andAco 2,fld 2,andGsnor 2on extra chromosome of trisomic-IV in linked associations.


1998 ◽  
Vol 123 (1) ◽  
pp. 98-103
Author(s):  
Maureen C. O'Leary ◽  
Thomas H. Boyle

Polyacrylamide gel electrophoresis was used to study inheritance and linkage of isozymes in Easter cactus (Hatiora species and interspecific hybrids). Five isozyme systems were analyzed: aspartate aminotransferase (AAT), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI). F1, F2, BC1, and S1 progeny were used for inheritance studies. Six polymorphic loci (Aat-1, Gpi-1, Mdh-1, Pgm-1, Pgm-2, and Tpi-2) were identified. Aat-1 and Pgm-1 were linked (recombination frequency = 26% ± 7%), but the other isozyme loci assorted independently. Aberrant segregation ratios were observed in at least one segregating family for all six isozyme loci. We hypothesize that segregation distortion was due to linkage between isozyme loci and other genes subject to pre- or postzygotic selection. The existence of five additional isozyme loci (Aat-2, Gpi-2, Mdh-2, Mdh-3, and Tpi-1) was inferred from segregation patterns and by comparison of isozyme profiles from phylloclades and pollen. These isozyme loci may prove useful for confirming hybridity in intra- and interspecific crosses, determining parentage of cultivars, and assessing genetic diversity in germplasm collections.


Genetics ◽  
1984 ◽  
Vol 108 (3) ◽  
pp. 669-679
Author(s):  
J M Martinez-Zapater ◽  
Jose L Oliver

ABSTRACT The genetic control of eight isozyme loci revealed by starch gel electrophoresis was studied through the analysis of three progenies derived from four tetraploid cultivars of Solanum tuberosum (groups Andigena and Tuberosum). Duplicate gene expression was found in seven (Got-A, Got-B, Pgd-C, Pgi-B, Pgm-A, Pgm-B and Pox-C) isozyme loci. In another isozyme gene (Adh-A), the parental genotypes were not adequate to distinguish between a monogenic or a digenic model of genetic control. Tetrasomic inheritance was demonstrated in four (Got-A, Got-B, Pgd-C and Pgi-B) isozyme loci. In the remaining duplicate genes, the parental genotypes precluded discrimination between disomic or tetrasomic models. Tetrasomic segregations of the chromosomal type were generally found; however, the isozyme phenotypes shown by three descendants from selfing cv. Katahdin indicate the occurrence of chromatid segregations, although aneuploidy cannot be ruled out. Either autoploidy or amphidiploidy with lack of chromosome differentiation between the two diploid ancestors can account for the existence of tetrasomic inheritance in the common potato.


1993 ◽  
Vol 86 (4) ◽  
pp. 417-426 ◽  
Author(s):  
K. Kazan ◽  
F. J. Muehlbauer ◽  
N. E. Weeden ◽  
G. Ladizinsky

1993 ◽  
Vol 73 (4) ◽  
pp. 917-926 ◽  
Author(s):  
R. E. Vaillancourt ◽  
A. E. Slinkard

Six new isozymes and three morphological markers were placed on the lentil (Lens culinaris L.) linkage map. The 17 isozymes and 11 morphological markers that were studied formed four linkage groups (I through IV). These linkage groups represent linkage relationships in the cultivated lentil (L. c. ssp. culinaris) and its wild progenitor (L. c. ssp. orientalis). However, in crosses between L. c. ssp. odemensis and other ssp. of L. culinaris, linkage relationships were slightly different. In these crosses markers of linkage groups III and IV showed disturbed segregation and pseudo-linkage. Although wide crosses contain more variability than narrow crosses in lentil, linkage relationships are more difficult to interpret because of the prevalence of disturbed segregation and pseudo-linkage. Key words: Lentil, Lens culinaris, linkage, isozyme, pseudo-linkage


Genetics ◽  
1978 ◽  
Vol 89 (1) ◽  
pp. 137-146
Author(s):  
Lila Ott ◽  
John G Scandalios

ABSTRACT Maize aminopeptidase is coded by four genes. Amp1 and Amp2 have been localized to chromosome 1. A three-point cross shows the gene order to be Amp2—15%—Amp1—33%—Adh2(alcohol dehydrogenase). Amp3 and Amp4 assort independently of each other and of chromosome 1 aminopeptidases. Another linkage relationship among the maize genes Amy2 (amylase), Cat1 (catalase), and Amp3 exists, but the chromosome location has yet to be established unequivocally.


1998 ◽  
Vol 123 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Jack E. Staub ◽  
Vladimir Meglic ◽  
James D. McCreight

Nineteen polymorphic and eleven monomorphic isozyme loci were identified in thirteen enzyme systems in a survey of four-hundred melon (Cucumis melo L.) accessions. Segregation of allozymes in F2 and backcross (BC) families for isozyme loci agreed with the expected 1:2:1 and 1:1 segregation ratios (P <0.01). Eleven isozyme loci were linked and were integrated to form a map containing two linkage groups spanning 98 cM with a mean linkage distance of ≈9 cM. Linkage groups (A and B) contain the following loci in the order: A Fdp-2, Pgd, Pgm, Mpi-1, Idh, and Ac, and B Pep-gl, Mdh-2, Mdh-4, Mdh-5, Mdh-6. The remaining eight loci (Acp-1, Acp-4, Ak-4, Fdp-1, Gpi, Mpi-2, Pep-la, and Pep-pap) segregated independently. The isozyme map constructed in this study provides genomic information for future linkage studies with economically important traits and concensus map construction through map merging.


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