Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.

Examining the Use of Police and Ambulance Data in Suicide Research

Background: Police and paramedics are often the first to respond to individuals in suicide crisis and have an important role to play in facilitating optimal care pathways. Yet, little evidence exists to inform these responses. Data linkage provides one approach to examining this knowledge gap. Aim: We identified studies that examined suicide behaviors and linked to police or ambulance data. Method: A systematic search of PubMed and Scopus was undertaken to identify data linkage studies that: (1) examined suicide behaviors, and (2) included police or ambulance data. Studies were reviewed to identify: aims; suicide behaviors examined; how these were measured; how the cohort was defined; topic area; and what datasets were linked. Results: Eight studies met the inclusion criteria. Six studies included police data, and two studies included ambulance data. No study included both. Two topic areas were identified: (1) suicide-related contact with police or ambulance services; and (2) associations between suicidal behaviors and violence, victimization, and criminality. Limitations: Limitations to the review include the potential to have missed studies that investigated or reported on suicidality under the guise of mental health problems; complexities and nuances arising from the role of police data in coronial investigations; and limitations in the number of databases searched. Conclusion: Police and ambulance data represent a currently underutilized source of valuable information relevant to suicide crises, and further research should aim to address this gap.

Closer to reality? The application of sequence analysis in crime linkage

Purpose Traditional crime linkage studies on serial sexual assaults have relied predominantly on a binary crime linkage approach that has yielded successful results in terms of linkage accuracy. Such an approach is a coarse reflection of reality by focussing mainly on the outcome of an offence, neglecting the forceful differences due to the intricate offender-victim interaction. Only few researchers have examined sexual assaults through the lens of a sequence analysis framework. This paper aims to present the first empirical test of offence sequence-based crime linkage, moving beyond exploratory analyses. Design/methodology/approach Offence accounts from 90 serial sexual assault and rape victims from the UK were analysed and sequentially coded. Sequence analysis allowed to compare all offences combinations regarding their underlying sequence of events. The resulting comparison was transformed and plotted in two-dimensional space by multidimensional scaling analysis for a visual inspection of linkage potential. The transformed proximities of all offences were used as predictors in a receiver operating characteristic analysis to actually test their discriminatory accuracy for crime linkage purpose. Findings Sequence analysis shows significant discriminatory accuracy for crime linkage purpose. However, the method does perform less well than previous binary crime linkage studies. Research limitations/implications Several limitations due to the nature of the data will be discussed. Practical implications The practical limitations are as follows: the study is a potential practical value for crime analysts; it is a complimentary methodology for statistical crime linkage packages; it requires automated coding to be useful; and it is very dependent on crime recoding standards. Originality/value The exploratory part of this study has been published in a book chapter in 2015. However, to the best of the authors’ knowledge, the succinct test of crime linkage accuracy is the first of its kind.