scholarly journals Flavonoid-Deficient Mutants in Grass Pea (Lathyrus sativusL.): Genetic Control, Linkage Relationships, and Mapping with Aconitase and S-Nitrosoglutathione Reductase Isozyme Loci

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Dibyendu Talukdar
Keyword(s):  
Genetic Analysis ◽  
Extra Chromosome ◽  
Recessive Inheritance ◽  
Linkage Studies ◽  
Grass Pea ◽  
Flavonoid Content ◽  
Banding Patterns ◽  
Trisomic Analysis ◽  
Isozyme Loci ◽  
Linkage Relationships

Two flavonoid-deficient mutants, designated asfldL-1andfldL-2, were isolated in EMS-mutagenized (0.15%, 10 h) M2progeny of grass pea (Lathyrus sativusL.). Both the mutants contained total leaf flavonoid content only 20% of their mother varieties. Genetic analysis revealed monogenic recessive inheritance of the trait, controlled by two different nonallelic loci. The two mutants differed significantly in banding patterns of leaf aconitase (ACO) and S-nitrosoglutathione reductase (GSNOR) isozymes, possessing unique bands inAco 1, Aco 2,andGsnor 2loci. Isozyme loci inherited monogenically showing codominant expression in F2(1 : 2 : 1) and backcross (1 : 1) segregations. Linkage studies and primary trisomic analysis mappedAco 1andfld 1loci on extra chromosome of trisomic-I andAco 2,fld 2,andGsnor 2on extra chromosome of trisomic-IV in linked associations.

scholarly journals Inheritance and Linkage Relationships of Melon (Cucumis melo L.) Isozymes

1998 ◽  
Vol 123 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Jack E. Staub ◽  
Vladimir Meglic ◽  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Linkage Studies ◽  
Genomic Information ◽  
Linkage Groups ◽  
Map Construction ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Cucumis Melo L ◽  
Linkage Relationships ◽  
Linkage Distance

Nineteen polymorphic and eleven monomorphic isozyme loci were identified in thirteen enzyme systems in a survey of four-hundred melon (Cucumis melo L.) accessions. Segregation of allozymes in F2 and backcross (BC) families for isozyme loci agreed with the expected 1:2:1 and 1:1 segregation ratios (P <0.01). Eleven isozyme loci were linked and were integrated to form a map containing two linkage groups spanning 98 cM with a mean linkage distance of ≈9 cM. Linkage groups (A and B) contain the following loci in the order: A Fdp-2, Pgd, Pgm, Mpi-1, Idh, and Ac, and B Pep-gl, Mdh-2, Mdh-4, Mdh-5, Mdh-6. The remaining eight loci (Acp-1, Acp-4, Ak-4, Fdp-1, Gpi, Mpi-2, Pep-la, and Pep-pap) segregated independently. The isozyme map constructed in this study provides genomic information for future linkage studies with economically important traits and concensus map construction through map merging.

Genetic Analysis of Chinese Cucumber Collections in the U.S. National Germplasm Collection

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 534e-534 ◽  
Author(s):  
J. Staub ◽  
Felix Sequen ◽  
Tom Horejsi ◽  
Jin Feng Chen
Keyword(s):  
Genetic Diversity ◽  
Principal Component Analysis ◽  
Genetic Variation ◽  
Genetic Analysis ◽  
Allelic Variation ◽  
Germplasm Collection ◽  
Principal Component ◽  
Isozyme Loci ◽  
National Plant Germplasm System ◽  
The U.S

Genetic variation in cucumber accessions from China was assessed by examining variation at 21 polymorphic isozyme loci. Principal component analysis of allelic variation allowed for the depiction of two distinct groupings of Chinese accessions collected in 1994 and 1996 (67 accessions). Six isozyme loci (Gpi, Gr, Mdh-2, Mpi-2, Pep-gl, and Pep-la) were important in elucidating these major groups. These groupings were different from a single grouping of Chinese 146 accessions acquired before 1994. Allelic variation in Chinese accessions allowed for comparisons with other accessions in the U.S. National Plant Germplasm System (U.S. NPGS) collection grouped by continent and sub-continent. When Chinese accessions taken collectively were compared with an array of 853 C. sativus U.S. NPGS accessions examined previously, relationships differed between accessions grouped by country or subcontinent. Data indicate that acquisition of additional Chinese and Indian cucumber accessions would be strategically important for increasing genetic diversity in the U.S. NPGS cucumber collection.

Inheritance and Linkage Studies with Five Isozyme Loci in Soybean 1

Crop Science ◽  
1987 ◽  
Vol 27 (5) ◽  
pp. 885-892 ◽  
Author(s):  
Jeffrey Griffin ◽  
Reid G. Palmer
Keyword(s):  
Linkage Studies ◽  
Isozyme Loci

scholarly journals Inheritance and Linkage Relationships of Isozymes in Easter Cactus

1998 ◽  
Vol 123 (1) ◽  
pp. 98-103
Author(s):  
Maureen C. O'Leary ◽  
Thomas H. Boyle
Keyword(s):  
Interspecific Hybrids ◽  
Triosephosphate Isomerase ◽  
Recombination Frequency ◽  
Polyacrylamide Gel Electrophoresis ◽  
Interspecific Crosses ◽  
Germplasm Collections ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Linkage Relationships ◽  
Aberrant Segregation

Polyacrylamide gel electrophoresis was used to study inheritance and linkage of isozymes in Easter cactus (Hatiora species and interspecific hybrids). Five isozyme systems were analyzed: aspartate aminotransferase (AAT), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI). F1, F2, BC1, and S1 progeny were used for inheritance studies. Six polymorphic loci (Aat-1, Gpi-1, Mdh-1, Pgm-1, Pgm-2, and Tpi-2) were identified. Aat-1 and Pgm-1 were linked (recombination frequency = 26% ± 7%), but the other isozyme loci assorted independently. Aberrant segregation ratios were observed in at least one segregating family for all six isozyme loci. We hypothesize that segregation distortion was due to linkage between isozyme loci and other genes subject to pre- or postzygotic selection. The existence of five additional isozyme loci (Aat-2, Gpi-2, Mdh-2, Mdh-3, and Tpi-1) was inferred from segregation patterns and by comparison of isozyme profiles from phylloclades and pollen. These isozyme loci may prove useful for confirming hybridity in intra- and interspecific crosses, determining parentage of cultivars, and assessing genetic diversity in germplasm collections.

scholarly journals Genetic Analysis of 11 Polymorphic Isozyme Loci in Chestnut Species and Characterization of Chestnut Cultivars by Multi-locus Allozyme Genotypes

1994 ◽  
Vol 119 (4) ◽  
pp. 840-849 ◽  
Author(s):  
Hongwen Huang ◽  
Fenny Dane ◽  
Joseph D. Norton
Keyword(s):  
Genetic Analysis ◽  
American Chestnut ◽  
Allozyme Polymorphism ◽  
Single Tree ◽  
European Chestnut ◽  
Enzyme Systems ◽  
Isozyme Loci ◽  
Slab Gels ◽  
Unique Species

Allozyme polymorphism in chestnut (Castanea) species was investigated using isoelectric focusing in thin-layer polyacrylamide slab gels. Genetic analysis of the progenies of intraspecific crosses and interspecific F2s and backcrosses (BC1s) allowed the verification of 11 polymorphic isozyme loci from 11 enzyme systems. The following loci were defined: Acp, Adh, Est-1, Est-2, Est-5, Me, Prx-1, Prx-2, Prx-3, Skd-3, and Skd-4. All polymorphic loci behaved as single-locus Mendelian genes. Skd showed unique species specificity. Skd-1 and Skd-2 were unique to the American chestnut (C. dentata Borkh.) and the European chestnut (C. sativa Mill.), whereas Skd-3 and Skd-4 were unique to the Chinese chestnut (C. mollissima Bl.) and the Japanese chestnut (C. crenata Sieb.). Linkage analysis revealed linkage for three pairs of loci: Skd-3/Skd-4, Est-1/Est-2, and Est-5/Prx-1. The single-tree progeny method was used successfully for isozyme genetic analysis. Forty-seven chestnut cultivars in six chestnut species were characterized using 12 isozyme loci and can be unambiguously identified by 12 multi-locus genotypes. The interspecific and geographic relationships among species were also discussed.

Features of Inheritance to Individual Traits of Millet (Panicum mileaceum L.)

2014 ◽  
Vol 1 (2) ◽  
pp. 45-51
Author(s):  
O. Rudnyk-Ivashchenko
Keyword(s):  
Genetic Analysis ◽  
Seed Size ◽  
Genetic Linkage ◽  
Morphological Traits ◽  
Seed Color ◽  
Individual Traits ◽  
New Generation ◽  
Linkage Relationships ◽  
Reliable Basis

Aim. To determine the inheritance of the millet morphological traits: seed color, size, anthocyan coloring of its parts, raсе specifi ed resistance to smut and genetic linkage relationships between these features, which are a reliable basis for the breeding of a new generation of varieties. Methods. Genetic analysis of the F1, F2 and F3 hybrids. Results. The interrelations between the seed size, its color and shape, and especially raсе specifi ed resistance to smut have been determined. Conclusions. The degree of linkage between the loci of genes that control the expression of the relevant characteristics in experimental combinations interbreeding has been stated.

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