scholarly journals Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Cinnamic Acid: Extraction of Cinnamic Acid from Spiked Blood Plasma

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Alvin Leong Joke Chow ◽  
Showkat Ahmad Bhawani
Keyword(s):  
Cinnamic Acid ◽  
Molecular Imprinting ◽  
Binding Capacity ◽  
Polymerization Reaction ◽  
Molar Ratio ◽  
Polymer Microspheres ◽  
Acid Extraction ◽  
Imprinted Polymer ◽  
Spiked Plasma

The molecular imprinting technique is used to create the molecularly imprinted polymers (MIPs) with higher binding capacity towards the template. In this research precipitation polymerization method with noncovalent approach was used to synthesize imprinted polymer microspheres. The polymerization reaction was conducted in a flask containing acetonitrile as a porogen, cinnamic acid as a template (T), acrylic acid (AA) as a monomer, divinylbenzene (DVB) as a cross-linker, and azobisisobutyronitrile as an initiator. The polymer particles were characterized by using SEM and FTIR. The rebinding efficiency was conducted by batch binding assay and the results were monitored by using HPLC. The batch binding results suggested MIP1 (T : AA : DVB, 1 : 6 : 20 molar ratio) is most suitable composition for the rebinding of cinnamic acid. The highly selective polymer (MIP1) was used for the extraction of cinnamic acid from human plasma. The extraction efficiency of imprinted polymer of cinnamic acid from spiked plasma was above 75%.

scholarly journals Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Piperine: Extraction of Piperine from Spiked Urine

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Rachel Marcella Roland ◽  
Showkat Ahmad Bhawani
Keyword(s):  
Extraction Efficiency ◽  
Binding Assay ◽  
Binding Capacity ◽  
Molar Ratio ◽  
Polymer Particles ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Ft Ir ◽  
Cross Linker

Molecularly imprinted polymer (MIP) microspheres for Piperine were synthesized by precipitation polymerization with a noncovalent approach. In this research Piperine was used as a template, acrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and 2,2′-azobisisobutyronitrile (AIBN) as an initiator and acetonitrile as a solvent. The imprinted and nonimprinted polymer particles were characterized by using Fourier transform infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The synthesized polymer particles were further evaluated for their rebinding efficiency by batch binding assay. The highly selected imprinted polymer for Piperine was MIP 3 with a composition (molar ratio) of 0.5 : 3 : 8, template : monomer : cross-linker, respectively. The MIP 3 exhibits highest binding capacity (84.94%) as compared to other imprinted and nonimprinted polymers. The extraction efficiency of highly selected imprinted polymer of Piperine from spiked urine was above 80%.

scholarly journals Enzymatic synthesis and characterization of novel terpolymers from renewable sources

2019 ◽  
Vol 91 (3) ◽  
pp. 397-408
Author(s):  
Diana Aparaschivei ◽  
Anamaria Todea ◽  
August E. Frissen ◽  
Valentin Badea ◽  
Gerlinde Rusu ◽  
...  
Keyword(s):  
Polymerization Reaction ◽  
Molar Ratio ◽  
Condensation Polymerization ◽  
Average Molecular Mass ◽  
New Class ◽  
Platform Chemicals ◽  
Starting Point ◽  
Reaction Cycles ◽  
Methylene Group

Abstract 2,5-Furandicarboxylic acid and itaconic acid are both important biobased platform chemicals and their terpolymer with 1,6-hexanediol (HDO) can be the starting point for a new class of reactive polyesters, with important applications. The green synthetic route developed in this study involves a biocatalytic condensation polymerization reaction of dimethyl furan-2,5-dicarboxylate (DMFDC) and dimethyl itaconate (DMI) with HDO in toluene at 80°C, using commercial immobilized lipases from Candida antarctica B. In the best conditions, the formed polymer product was isolated with more than 80% yield, containing about 85% terpolymer with average molecular mass of about 1200 (Mn, calculated from MALDI-TOF MS data) and 15% DMFDC_HDO copolymer. Considering the higher reactivity of DMFDC, the composition of the synthesized polymer can be directed by adjusting the molar ratio of DMFDC and DMI, as well as by extending the reaction time. Structural analysis by NMR demonstrated the regioselective preference for the carbonyl group from DMI adjacent to the methylene group. The biocatalyst was successfully reused in multiple reaction cycles.

Synthesis and characterization of molecularly imprinted polymer microspheres functionalized with POSS

2020 ◽  
Vol 511 ◽  
pp. 145506 ◽  
Author(s):  
Jianwei Bai ◽  
Yunan Zhang ◽  
Wenpan Zhang ◽  
Xiaofei Ma ◽  
Yawei Zhu ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Synthesis And Characterization ◽  
Polymer Microspheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

scholarly journals Selective Enrichment of Clenbuterol onto Molecularly Imprinted Polymer Microspheres with Tailor-made Structure and Oxygen Functionalities

Polymers ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 1635 ◽  
Author(s):  
Xiangyun Zhao ◽  
Yuliang Mai ◽  
Dongchu Chen ◽  
Min Zhang ◽  
Huawen Hu
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymer ◽  
Photoelectron Spectroscopy ◽  
Solid Phase ◽  
Feed Additive ◽  
Extraction Column ◽  
Polymer Microspheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Enrichment

The noxious clenbuterol misapplied as the feed additive has posed an enormous threat to humans who actively rely on the food chains with high potential of contamination by clenbuterol, such as pork and beef. It is, therefore, highly desirable to develop novel materials and strategies for dealing with the clenbuterol. Herein, functional polymer microspheres prepared by Pickering emulsion polymerization were explored for the selective enrichment of the clenbuterol, and their structure and oxygen functionalities could be tailor-made by a molecular imprinting process. The clenbuterol imprinting was adequately demonstrated to not only increase the particle size (~52 nm vs. ~42 nm) and create cavities for the accommodation of the clenbuterol molecules, but also reduce the oxygen functionalities of the resulting molecularly imprinted polymer microspheres (MIPMs) by approximately 4 at.%, which is believed to correlate with the high specificity of the MIPMs. Various characterization methods were employed to evidence these findings, including scanning electron microscopy, BET measurements, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and elemental mapping examination. More importantly, the MIPMs showed a markedly superior enrichment capability towards clenbuterol to the counterpart, that is, non-molecularly imprinted polymer microspheres (NIPMs). Compared to the NIPMs without specificity for clenbuterol, the MIPMs exhibited an impressive selectivity to clenbuterol, with the relative selectivity coefficient (k′) values largely exceeding 1, thus corroborating that the useful molecular imprinting led to the generation of the binding sites complementary to the clenbuterol molecule in the size and functionalities. The MIPMs were also employed as the stationary phase to fabricate molecularly imprinting solid-phase extraction column, and the spike recovery was demonstrated to be not significantly decreased even after nine cycles. Furthermore, the reliability of the method was also evidenced through the comparison of the MIPMs prepared from different batches.

scholarly journals Synthesis and Characterization of a Molecularly Imprinted Polymer of Spermidine and the Exploration of Its Molecular Recognition Properties

Polymers ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 1389 ◽  
Author(s):  
Yu-Jie Huang ◽  
Rui Chang ◽  
Qiu-Jin Zhu
Keyword(s):  
Molar Ratio ◽  
Template Molecule ◽  
Functional Monomer ◽  
Imprinted Polymer ◽  
Polymer Complexes ◽  
Molecular Imprinting Technology ◽  
Recognition Ability ◽  
Scatchard Equation ◽  
Factor Α

Spermidine is a functional ingredient that can extend the lifespan of many foods and indicate meat safety. However, its synthesis and enrichment is expensive and complex. To develop an effective separation material that can offer highly selective recognition of spermidine, we first applied non-covalent molecular imprinting technology using methacrylic acid as a functional monomer, azobisisobutyronitrile as an initiator, and ethylene glycol dimethacrylate as a cross-linker. The adsorption properties of the polymers were analyzed using the Scatchard equation, the Lagergren kinetic equation, and the static distribution coefficient. The optimal polymerization molar ratio of the template molecule spermidine to the functional monomer was 1:4, the maximum adsorption amount was 97.75 μmol/g, and the adsorption equilibrium time was 300 min. The selective experiment showed that the interfering substances tyramine and histamine had selectivity factor α values of 2.01 and 1.78, respectively, indicating that the prepared polymer had good spermidine recognition ability. The density function theory calculations showed that the hydrogen bond strength, steric effect, and product energy caused adsorption and separation differences among the different imprinted polymer complexes.

Synthesis of nicotinamide-imprinted polymers and their binding performances in organic and aqueous media

e-Polymers ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Pattarawarapan Mookda ◽  
Komkham Singha ◽  
Kareuhanon Weeranuch ◽  
Tayapiwatana Chatchai
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Capacity ◽  
Aqueous Media ◽  
Bulk Polymerization ◽  
Molar Ratio ◽  
Imprinted Polymer ◽  
Isolation And Identification ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers

AbstractTo obtain molecularly imprinted polymers capable of selective rebinding with nicotinamide (NAM), NAM imprinted polymers were synthesized via bulk polymerization using various functional monomers and cross-linkers. The NAM recognition properties of these polymers were investigated in organic and aqueous solvents by equilibrium rebinding experiments. The results show that the imprinted polymer prepared using 1:4:4 molar ratio of NAM/MAA/TRIM in dichloromethane exhibited the greatest NAM binding capacity and selectivity. This polymer is potentially valuable for the analysis of NAM in complex matrices where selective isolation and identification are needed.

Preparation and Characterization of Molecularly Imprinted Polymer Microspheres of p-Nitroaniline

2011 ◽  
Vol 71-78 ◽  
pp. 3411-3414
Author(s):  
Ming Yang ◽  
Qian Sun ◽  
Cai Yun Li
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Single Step ◽  
Polymer Microspheres ◽  
Polystyrene Microspheres ◽  
Imprinted Polymer ◽  
Molecular Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Uv Visible ◽  
Polymerization Method

Polystyrene microspheres were prepared by the emulsifier-free polymerization method. Using the polystyrene microspheres as seeds, p-nitroaniline as template molecules, the monodispersed molecularly imprinted polymer microspheres (MIPMs) were prepared by a single-step swelling and polymerization method. The monodispersed molecular imprinted polymers with porous structure were characterized by SEM, and UV-visible spectrophotometry. The adsorption performance of MIPMs for p-nitroaniline was investigated.

Molecularly Imprinted Polymer Microspheres Prepared by Precipitation Polymerization for Atenolol Recognition

2010 ◽  
Vol 148-149 ◽  
pp. 1192-1198 ◽  
Author(s):  
Jin Yang Yu ◽  
Xiao Ling Hu ◽  
Ren Yuan Song ◽  
Shan Xi
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Binding Sites ◽  
Binding Capacity ◽  
Precipitation Polymerization ◽  
Acetonitrile Solution ◽  
Template Molecule ◽  
Affinity Binding ◽  
Polymer Microspheres ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

Molecularly imprinted polymer microspheres for selective binding and recognition of atenolol were prepared by means of precipitation polymerization method using methacylic acid as functional monomer and trimethylolpropane trimethacrylate as cross-linker in the presence of atenolol as template molecule in acetonitrile solution. Computer simulation was employed to demonstrate the mechanism of the interaction between methacylic acid and atenolol. The scanning electron microscopy exhibited that the polymers were uniform spheres with the diameter of about 0.6µm. The adsorption properties of atenolol for imprinted microspheres were evaluated by equilibrium rebinding experiments. Scatchard plot analysis revealed that there were two classes of binding sites in the imprinted microspheres. The dissociation constant and the apparent maximum binding capacity were 4.56×10-4mol/L and 186.46μmol/g for the high affinity binding sites, 2.40×10-2mol/L and 4.01mmol/g for the low affinity binding sites. Compared to the structrally analogues, the imprinted microspheres exhibited a high selective reconizable capacity towards the template.

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