scholarly journals Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Piperine: Extraction of Piperine from Spiked Urine

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Rachel Marcella Roland ◽  
Showkat Ahmad Bhawani
Keyword(s):  
Extraction Efficiency ◽  
Binding Assay ◽  
Binding Capacity ◽  
Molar Ratio ◽  
Polymer Particles ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Ft Ir ◽  
Cross Linker

Molecularly imprinted polymer (MIP) microspheres for Piperine were synthesized by precipitation polymerization with a noncovalent approach. In this research Piperine was used as a template, acrylic acid as a functional monomer, ethylene glycol dimethacrylate as a cross-linker, and 2,2′-azobisisobutyronitrile (AIBN) as an initiator and acetonitrile as a solvent. The imprinted and nonimprinted polymer particles were characterized by using Fourier transform infrared spectroscopy (FT-IR) and Scanning Electron Microscopy (SEM). The synthesized polymer particles were further evaluated for their rebinding efficiency by batch binding assay. The highly selected imprinted polymer for Piperine was MIP 3 with a composition (molar ratio) of 0.5 : 3 : 8, template : monomer : cross-linker, respectively. The MIP 3 exhibits highest binding capacity (84.94%) as compared to other imprinted and nonimprinted polymers. The extraction efficiency of highly selected imprinted polymer of Piperine from spiked urine was above 80%.

scholarly journals Synthesis and Characterization of Molecular Imprinting Polymer Microspheres of Cinnamic Acid: Extraction of Cinnamic Acid from Spiked Blood Plasma

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Alvin Leong Joke Chow ◽  
Showkat Ahmad Bhawani
Keyword(s):  
Cinnamic Acid ◽  
Molecular Imprinting ◽  
Binding Capacity ◽  
Polymerization Reaction ◽  
Molar Ratio ◽  
Polymer Microspheres ◽  
Acid Extraction ◽  
Imprinted Polymer ◽  
Spiked Plasma

The molecular imprinting technique is used to create the molecularly imprinted polymers (MIPs) with higher binding capacity towards the template. In this research precipitation polymerization method with noncovalent approach was used to synthesize imprinted polymer microspheres. The polymerization reaction was conducted in a flask containing acetonitrile as a porogen, cinnamic acid as a template (T), acrylic acid (AA) as a monomer, divinylbenzene (DVB) as a cross-linker, and azobisisobutyronitrile as an initiator. The polymer particles were characterized by using SEM and FTIR. The rebinding efficiency was conducted by batch binding assay and the results were monitored by using HPLC. The batch binding results suggested MIP1 (T : AA : DVB, 1 : 6 : 20 molar ratio) is most suitable composition for the rebinding of cinnamic acid. The highly selective polymer (MIP1) was used for the extraction of cinnamic acid from human plasma. The extraction efficiency of imprinted polymer of cinnamic acid from spiked plasma was above 75%.

scholarly journals Synthesis and Characterization of Molecularly Imprinted Polymer for the Removal/Extraction of Thymol from Spiked Blood Serum and River water

2019 ◽  
Vol 31 (11) ◽  
pp. 2479-2484
Author(s):  
Salma Bakhtiar ◽  
Showkat Ahmad Bhawani ◽  
Syed Rizwan Shafqat
Keyword(s):  
Blood Serum ◽  
River Water ◽  
Selectivity Coefficient ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Structural Analogue ◽  
Uniform Size ◽  
Molecularly Imprinted ◽  
Ft Ir

Molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization using thymol as a template molecule, acrylamide as a functional monomer and N,N-methylbisacrylamide as the crosslinker with a non-covalent approach. The polymers were characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), fourier-transform infra red spectroscopy(FT-IR) and Brunauer-Emmett-Teller (BET). The SEM results depicted that the shape of polymer particles is spherical with uniform size (micro-meters). The BET results also showed better surface area, pore size and pore volume of MIP as compared to non-imprinted polymer (NIP). A series of parameters such as initial concentration, polymer dosage, effect of pH and slectivity with structural analogue were conducted. The selectivity of MIP towards thymol was appreciable as compared to its structural analogue gallic acid with a relative selectivity coefficient of 3.59. Finally, MIP has been successfully used for extraction of thymol from the spiked blood serum (84 %) and river water sample (98 %).

Synthesis of nicotinamide-imprinted polymers and their binding performances in organic and aqueous media

e-Polymers ◽  
2008 ◽  
Vol 8 (1) ◽  
Author(s):  
Pattarawarapan Mookda ◽  
Komkham Singha ◽  
Kareuhanon Weeranuch ◽  
Tayapiwatana Chatchai
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Capacity ◽  
Aqueous Media ◽  
Bulk Polymerization ◽  
Molar Ratio ◽  
Imprinted Polymer ◽  
Isolation And Identification ◽  
Molecularly Imprinted ◽  
Functional Monomers ◽  
Imprinted Polymers

AbstractTo obtain molecularly imprinted polymers capable of selective rebinding with nicotinamide (NAM), NAM imprinted polymers were synthesized via bulk polymerization using various functional monomers and cross-linkers. The NAM recognition properties of these polymers were investigated in organic and aqueous solvents by equilibrium rebinding experiments. The results show that the imprinted polymer prepared using 1:4:4 molar ratio of NAM/MAA/TRIM in dichloromethane exhibited the greatest NAM binding capacity and selectivity. This polymer is potentially valuable for the analysis of NAM in complex matrices where selective isolation and identification are needed.

Synthesis and characterization of a propazine imprinted polymer for the extraction of triazines herbicides

2008 ◽  
Vol 57 (1) ◽  
pp. 139-144 ◽  
Author(s):  
L. Guzzella ◽  
F. Pozzoni ◽  
C. Baggiani
Keyword(s):  
Solid Phase ◽  
Soxhlet Extraction ◽  
Recovery Efficiency ◽  
Imprinted Polymer ◽  
Residual Concentration ◽  
Molecularly Imprinted ◽  
Cross Linker ◽  
Data Extrapolation ◽  
River Po

A MIP (molecularly imprinted polymer) was synthesized and evaluated for its use as sorbent for solid phase extraction (MISPE) of common used triazines (atrazine and terbuthylazine) and their widespread metabolites (desethyl-atrazine and desethyl-terbuthylazine) in water samples. MIP was produced by bulk polymerisation using methacrylic acid as functional monomer, ethylene glycol dimethacrylate as cross-linker, propazine as template and toluene as porogen solvent. Different washing methods of the synthesized polymer were evaluated. Soxhlet extraction provided the best results with a residual concentration of propazine, ranging from 0.78 to 2.86 μg for 1 g of polymer. Capacity factor was calculated using a 5 cm HPLC column filled with MIP and NIP (Not Imprinted Polymer): data extrapolation indicated a log Kw of 4.3 for MIP and a log Kw of 3.5 for NIP. Also frontal analyses confirmed a different behaviour of the two polymers. By comparing the recovery efficiency of MIP with that of traditional LiChrolut EN cartridge in the extraction of a River Po water sample, the results confirmed the reliability of this new technique for the analysis of herbicide compounds.

scholarly journals Sintesis dan Karakterisasi Molecularly Imprinted Polymer Untuk Kloramfenikol Menggunakan Polimerisasi Fasa Ruah

2018 ◽  
Vol 3 (1) ◽  
pp. 40-46
Author(s):  
Rosita Dwi Chrisnandari
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Fourier Transform Infrared Spectroscopy ◽  
Molecularly Imprinted Polymer ◽  
Fourier Transform Infrared ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Ft Ir ◽  
Cross Linker

Kloramfenikol adalah antibiotik yang memiliki aktivitas untuk melawan bakteri gram positif dan gram negatif yang pemakaiannya dibatasi dan diawasi oleh lembaga pemerintahan karena memiliki efek samping yang membahayakan kesehatan manusia dan lingkungan. Metode analisis kloramfenikol yang digunakan umumnya kurang selektif dan kurang spesifik terhadap kloramfenikol. Pada penelitian ini dikembangkan teknik preparasi sampel berbasis molecularly imprinted polymer (MIP) yang dapat digunakan sebagai adsorben selektif dan spesifik terhadap kloramfenikol. MIP disintesis menggunakan kloramfenikol sebagai template, asam metakrilat (MAA) sebagai monomer, etilen glikol dimetakrilat (EGDMA) sebagai cross-linker dan kloroform sebagai porogen dengan perbandingan mol rasio template:monomer:cross-linker sebesar 1:4:20 secara polimerisasi fasa ruah pada suhu 70 ºC selama 11 jam. Elusi template dilakukan melalui ekstraksi pelarut menggunakan metanol:asam asetat 9:1 (v/v). Non-imprinted polymer (NIP) dan polimer kontrol juga disintesis sebagai pembanding. Hasil polimerisasi dikarakterisasi menggunakan fourier transform infrared spectroscopy (FT-IR) dan adsorpsi-desorpsi N2. Spektra FT-IR menunjukkan gugus fungsi spesifik kloramfenikol pada MIP tidak tampak bila dibandingkan dengan spektra NIP. Luas permukaan MIP diketahui sebesar 106,609 m2/g dan 41,860 m2/g untuk polimer kontrol. Hal ini membuktikan bahwa MIP memiliki sisi pengenal yang lebih banyak dan spesifik dibandingkan dengan polimer kontrol. MIP hasil sintesis dapat digunakan untuk meningkatkan selektivitas metode analisis kloramfenikol secara kromatografi.Kata kunci: Kloramfenikol, Molecularly Imprinted Polymer (MIP), Polimerisasi Fasa Ruah

scholarly journals Synthesis of Molecularly Imprinted Polymer for the Removal of Melamine

2019 ◽  
Vol 31 (12) ◽  
pp. 2770-2776
Author(s):  
Rachel Marcella Roland ◽  
Showkat Ahmad Bhawani
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Selectivity Coefficient ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Transmission Electron ◽  
Ir Transmission ◽  
Ft Ir ◽  
Cross Linker ◽  
Relative Selectivity

In this article, molecularly imprinted polymer (MIP) of melamine was synthesized by non-covalent approach using microemulsion medium. In this study, melamine is used as a template molecule, acrylic acid as a functional monomer, N,N′-methylenebis(acrylamide) (MBAm) as a cross-linker and 2,2′-azobisisobutyronitrile (AIBN) as an initiator and microemulsion as a solvent. The produced polymers were characterized by Fourier transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), Brunauer-Emmett-Teller (BET) and thermogravimetric analysis (TGA). The batch binding analysis was conducted for the evaluation of MIP and NIP rebinding efficiency with the melamine template. The adsorption studies including the adsorbent dosage of polymer, initial concentrations and pH of melamine solution were successfully carried out on both MIP and NIP. For selectivity test, 2,4,6-trichlorophenol was chosen as the competitive molecule against melamine template. A very good relative selectivity coefficient was achieved. The removal of melamine from different samples were conducted successfully by achieving a good removal efficiency in milk, river water and human blood serum 84.30, 94.26 and 93.32 %, respectively.

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