scholarly journals Association of Polymorphisms of Phase I Metabolizing Genes with Sister Chromatid Exchanges in Occupational Workers Exposed to Toluene Used in Paint Thinners

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kanu Priya ◽  
Anita Yadav ◽  
Neeraj Kumar ◽  
Sachin Gulati ◽  
Neeraj Aggarwal ◽  
...  
Keyword(s):  
Phase I ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Sister Chromatid Exchanges ◽  
Confounding Factors ◽  
Genetic Damage ◽  
Blood Samples ◽  
Genotoxic Risk ◽  
Chromatid Exchanges

This study investigated genetic damage in paint workers mainly exposed to toluene as it is a major solvent used in paint thinners. Sister chromatid exchange (SCE) assay was used as biomarker of genotoxicity. Blood samples were collected from 30 paint workers and 30 control subjects matched with respect to age and other confounding factors except for exposure to toluene. SCE frequency was found to be significantly higher in paint workers (4.81±0.92) as compared to control individuals (1.73±0.54) (p<0.05). We also investigated influence of polymorphisms of CYP2E1 and CYP1A1m2 genes on SCE frequency. Our results showed that there was significant increase in frequencies of SCE among the mutant genotypes of CYP2E1 and CYP1A1m2 as compared to wild genotypes. Our study indicated that long term exposure of toluene can increase genotoxic risk in paint workers.

Screening of Chromosomal Aberrations and Sister Chromatid Exchanges in Peripheral Lymphocytes of Vegetable Garden Workers

1988 ◽  
Vol 7 (4) ◽  
pp. 333-336 ◽  
Author(s):  
D.S. Rupa ◽  
P. Rita ◽  
P.P. Reddy ◽  
O.S. Reddi
Keyword(s):  
Chromosomal Aberrations ◽  
Sister Chromatid ◽  
Sister Chromatid Exchanges ◽  
Age Group ◽  
Socioeconomic Class ◽  
Blood Samples ◽  
Peripheral Lymphocytes ◽  
Occupationally Exposed ◽  
Chromatid Exchanges ◽  
Healthy Males

1 Twenty-five male workers occupationally exposed to DDT, BHC malathion, parathion, dimethoate, fenitrothion, urea and gromor were selected as subjects for the analysis of chromosomal aberrations and sister chromatid exchanges (SCE) in peripheral lymphocytes. 2 Blood samples were collected from 30 normal healthy males from the same age group and socioeconomic class for the control. 3 The frequency of chromosomal aberrations and SCEs increased significantly irrespective of the duration of exposure to pesticides, when compared to controls.

Do Non-Steroidal Anti-Inflammatory Drugs Induce Sister Chromatid Exchanges in T Lymphocytes?

1996 ◽  
Vol 24 (1) ◽  
pp. 84-87 ◽  
Author(s):  
Y Ozkul ◽  
A Erenmemisoglu ◽  
A Ekecik ◽  
C Saatci ◽  
S Ozdamar ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Human Lymphocytes ◽  
Sister Chromatid Exchanges ◽  
Before And After ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Chromatid Exchanges ◽  
Exchange Technique

The genetic toxicity of non-steroidal anti-inflammatory drugs was investigated using the sister chromatid exchange technique in cultured human lymphocytes. A total of 48 patients were treated with non-steroidal anti-inflammatory drugs (ibuprofen, ketoprofen, naproxen, indomethacin, diclofenac or acetylsalicylic acid) for 2 weeks. The average numbers of sister chromatid exchanges in cultured lymphocytes from the patients, before and after treatment with these drugs, did not differ significantly ( P > 0.05). These results indicate that treatment with non-steroidal anti-inflammatory drugs for 2 weeks does not induce sister chromatid exchanges in T lymphocytes.

New staining method for the detection of sister-chromatid exchanges in BrdU-labelled chromosomes

1978 ◽  
Vol 32 (1) ◽  
pp. 185-195
Author(s):  
P. Alves ◽  
J. Jonasson
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Centromeric Region ◽  
Staining Method ◽  
Cytological Study ◽  
Sister Chromatid Exchanges ◽  
Selective Staining ◽  
Chromatid Exchanges

A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.

scholarly journals Automatic measurement of sister chromatid exchange frequency.

1977 ◽  
Vol 25 (7) ◽  
pp. 741-753 ◽  
Author(s):  
G W Zack ◽  
W E Rogers ◽  
S A Latt
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Detection System ◽  
Automatic Measurement ◽  
Sister Chromatid Exchanges ◽  
Metaphase Chromosomes ◽  
Exchange Frequency ◽  
Sister Chromatid Exchange Frequency ◽  
Chromatid Exchanges ◽  
Microscope Slides

An automatic system for detecting and counting sister chromatid exchanges in human chromosomes has been developed. Metaphase chromosomes from lymphocytes which had incorporated 5-bromodeoxyuridine for two replication cycles were treated with the dye 33258 Hoechst and photodegraded so that the sister chromatids exhibited differential Giemsa staining. A computer-controlled television-microscope system was used to acquire digitized metaphase spread images by direct scanning of microscope slides. Individual objects in the images were identified by a thresholding procedure. The probability that each object was a single, separate chromosome was estimated from size and shape measurements. An analysis of the spatial relationships of the dark-chromatid regions of each object yielded a set of possible exchange locations and estimated probabilities that such locations corresponded to sister chromatid exchanges. A normalized estimate of the sister chromatid exchange frequency was obtained by summing the joint probabilities that a location contained an exchange within a single, separate chromosome over the set of chromosomes from one or more cells and dividing by the expected value of the total chromosome area analyzed. Comparison with manual scoring of exchanges showed satisfactory agreement up to levels of approximately 30 sister chromatid exchanges/cell, or slightly more than twice control levels. The processing time for this automated sister chromatid exchange detection system was comparable to that of manual scoring.

scholarly journals Evaluation of Sister Chromatid Exchanges and Hypoxanthine guanine phosphoribosyl transferase Gene Mutation Assay in Peripheral Lymphocytes of Workers Exposed to Radiation at Al-Tuwaitha Site

2015 ◽  
Vol 39 (2) ◽  
pp. 62-65
Author(s):  
Abdulsahib K. Ali
Keyword(s):  
Ionizing Radiation ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Cumulative Effect ◽  
Sister Chromatid Exchanges ◽  
Control Group ◽  
Blood Samples ◽  
Phosphoribosyl Transferase ◽  
Mutation Assay ◽  
Hypoxanthine Guanine Phosphoribosyl Transferase

     The present study aims at using the biological techniques in a genotoxicity assessment of low doses of radiation in samples of workers in Al-Tuwaitha site due to decommissioning to radioactive contamination as a result of work. This study included 50 male blood samples, aged 35 - 63 years as well as 35 blood samples from non-smokers and non-alcoholic as control group which included 25 male and 10 female aged 25 -57 years. The endpoints used were sister chromatid exchange (SCE) and hypoxanthine guanine phosphoribosyl transferase (HPRT) mutation assay. The sister chromatid exchange in the human lymphocyte for radiation worker was significantly (P<0.05) higher than in the control group. While the results of mutation for HPRT were non-significant when compared with the control group. In conclusion, the results indicated the possibility of using the changes in sister chromatid exchange as useful biomarkers for the detection of human exposure to ionizing radiation. In conclusion, the increase frequencies of sister chromatid exchange in radiation workers indicate the cumulative effect of low-level chronic exposure to ionizing radiation.

Sign in / Sign up

Export Citation Format

Share Document