New staining method for the detection of sister-chromatid exchanges in BrdU-labelled chromosomes

1978 ◽  
Vol 32 (1) ◽  
pp. 185-195
Author(s):  
P. Alves ◽  
J. Jonasson
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Centromeric Region ◽  
Staining Method ◽  
Cytological Study ◽  
Sister Chromatid Exchanges ◽  
Selective Staining ◽  
Chromatid Exchanges

A direct Giemsa technique is described for the selective staining of Brd-U-substituted chromatin. It can advantageously be used for sister-chromatid exchange analysis and for the detailed cytological study of the mouse kinetochores. Presumptive inversions within the centromeric region of chromosomes 4 and 18 in the C57Black/6J mouse are described.

Do Non-Steroidal Anti-Inflammatory Drugs Induce Sister Chromatid Exchanges in T Lymphocytes?

1996 ◽  
Vol 24 (1) ◽  
pp. 84-87 ◽  
Author(s):  
Y Ozkul ◽  
A Erenmemisoglu ◽  
A Ekecik ◽  
C Saatci ◽  
S Ozdamar ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Human Lymphocytes ◽  
Sister Chromatid Exchanges ◽  
Before And After ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Chromatid Exchanges ◽  
Exchange Technique

The genetic toxicity of non-steroidal anti-inflammatory drugs was investigated using the sister chromatid exchange technique in cultured human lymphocytes. A total of 48 patients were treated with non-steroidal anti-inflammatory drugs (ibuprofen, ketoprofen, naproxen, indomethacin, diclofenac or acetylsalicylic acid) for 2 weeks. The average numbers of sister chromatid exchanges in cultured lymphocytes from the patients, before and after treatment with these drugs, did not differ significantly ( P > 0.05). These results indicate that treatment with non-steroidal anti-inflammatory drugs for 2 weeks does not induce sister chromatid exchanges in T lymphocytes.

scholarly journals Association of Polymorphisms of Phase I Metabolizing Genes with Sister Chromatid Exchanges in Occupational Workers Exposed to Toluene Used in Paint Thinners

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kanu Priya ◽  
Anita Yadav ◽  
Neeraj Kumar ◽  
Sachin Gulati ◽  
Neeraj Aggarwal ◽  
...  
Keyword(s):  
Phase I ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Sister Chromatid Exchanges ◽  
Confounding Factors ◽  
Genetic Damage ◽  
Blood Samples ◽  
Genotoxic Risk ◽  
Chromatid Exchanges

This study investigated genetic damage in paint workers mainly exposed to toluene as it is a major solvent used in paint thinners. Sister chromatid exchange (SCE) assay was used as biomarker of genotoxicity. Blood samples were collected from 30 paint workers and 30 control subjects matched with respect to age and other confounding factors except for exposure to toluene. SCE frequency was found to be significantly higher in paint workers (4.81±0.92) as compared to control individuals (1.73±0.54) (p<0.05). We also investigated influence of polymorphisms of CYP2E1 and CYP1A1m2 genes on SCE frequency. Our results showed that there was significant increase in frequencies of SCE among the mutant genotypes of CYP2E1 and CYP1A1m2 as compared to wild genotypes. Our study indicated that long term exposure of toluene can increase genotoxic risk in paint workers.

scholarly journals Automatic measurement of sister chromatid exchange frequency.

1977 ◽  
Vol 25 (7) ◽  
pp. 741-753 ◽  
Author(s):  
G W Zack ◽  
W E Rogers ◽  
S A Latt
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Detection System ◽  
Automatic Measurement ◽  
Sister Chromatid Exchanges ◽  
Metaphase Chromosomes ◽  
Exchange Frequency ◽  
Sister Chromatid Exchange Frequency ◽  
Chromatid Exchanges ◽  
Microscope Slides

An automatic system for detecting and counting sister chromatid exchanges in human chromosomes has been developed. Metaphase chromosomes from lymphocytes which had incorporated 5-bromodeoxyuridine for two replication cycles were treated with the dye 33258 Hoechst and photodegraded so that the sister chromatids exhibited differential Giemsa staining. A computer-controlled television-microscope system was used to acquire digitized metaphase spread images by direct scanning of microscope slides. Individual objects in the images were identified by a thresholding procedure. The probability that each object was a single, separate chromosome was estimated from size and shape measurements. An analysis of the spatial relationships of the dark-chromatid regions of each object yielded a set of possible exchange locations and estimated probabilities that such locations corresponded to sister chromatid exchanges. A normalized estimate of the sister chromatid exchange frequency was obtained by summing the joint probabilities that a location contained an exchange within a single, separate chromosome over the set of chromosomes from one or more cells and dividing by the expected value of the total chromosome area analyzed. Comparison with manual scoring of exchanges showed satisfactory agreement up to levels of approximately 30 sister chromatid exchanges/cell, or slightly more than twice control levels. The processing time for this automated sister chromatid exchange detection system was comparable to that of manual scoring.

Chromosomal Aberrations and Sister-Chromatid Exchange Frequencies in Workers Occupationally Exposed to Textile Dyes

1992 ◽  
Vol 11 (4) ◽  
pp. 275-277 ◽  
Author(s):  
R. Mohan Kumar ◽  
R. Chandrasekar ◽  
M.V.U. Rani
Keyword(s):  
Chromosomal Aberrations ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Sister Chromatid Exchanges ◽  
Textile Dyes ◽  
Female Workers ◽  
Peripheral Lymphocytes ◽  
Occupationally Exposed ◽  
Chromatid Exchanges

The peripheral lymphocytes of 11 male and seven female workers occupationally exposed to textile dyes were studied for cytogenetic change. A significant increase in the frequency of chromosomal aberrations and sister chromatid exchanges were recorded regardless of the duration of the workers' exposure to the dyes.

Diagnostic Ultrasound and Sister Chromatid Exchanges

1985 ◽  
Vol 40 (9) ◽  
pp. 589-590
Author(s):  
V. CIARAVINO ◽  
A. BRULFERT ◽  
M. W. MILLER ◽  
D. JACOBSON-KRAM ◽  
W. F. MORGAN
Keyword(s):  
Sister Chromatid ◽  
Diagnostic Ultrasound ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges

scholarly journals PRESENCE OF A BASE LINE LEVEL OF SISTER CHROMATID EXCHANGES IN SOMATIC CELLS OF DROSOPHILA MELANOGASTER IN VIVO AND IN VITRO

Genetics ◽  
1982 ◽  
Vol 100 (2) ◽  
pp. 259-278
Author(s):  
Hideo Tsuji
Keyword(s):  
Drosophila Melanogaster ◽  
Sister Chromatid ◽  
Ganglion Cells ◽  
Sister Chromatid Exchanges ◽  
Base Line ◽  
Cell Cycles ◽  
Chromatid Exchanges ◽  
Third Instar Larvae

ABSTRACT Sister chromatid exchanges (SCEs) under in vivo and in vitro conditions were examined in ganglion cells of third-instar larvae of Drosophila melanogaster (Oregon-R). In the in vivo experiment, third-instar larvae were fed on synthetic media containing 5-bromo-2′-deoxyuridine (BrdUrd). After two cell cycles, ganglia were dissected and treated with colchicine. In the in vitro experiment, the ganglia were also incubated in media containing BrdUrd for two cell cycles, and treated with colchicine. SCEs were scored in metaphase stained with Hoechst 33258 plus Giemsa. The frequencies of SCEs stayed constant in the range of 25-150 vg/ml and 0.25-2.5 vg/ml of BrdUrd in vivo and in vitro, respectively. SCEs gradually increased at higher concentrations, strongly suggesting that at least a fraction of the detected SCEs are spontaneous. The constant levels of SCE frequency were estimated, on the average, at 0.103 per cell per two cell cycles for females and 0.101 for males in vivo and at 0.096 for females and 0.091 for males in vitro. No difference was found in the SCE frequency between sexes at any of the BrdUrd concentrations. The analysis for the distribution of SCEs within chromosomes revealed an extraordinarily high proportion of the SCEs at the junctions between euchromatin and heterochromatin; the remaining SCEs were preferentially localized in the euchromatic regions of the chromosomes and in the heterochromatic Y chromosome. These results were largely inconsistent with those of Gatti et al. (1979).

scholarly journals Sister chromatid exchanges and structural chromosome aberrations in relation to smoking in 91 individuals

Hereditas ◽  
2008 ◽  
Vol 98 (1) ◽  
pp. 77-81 ◽  
Author(s):  
K. HEDNER ◽  
B. HÖGSTEDT ◽  
A.-M. KOLNIG ◽  
E. MARK-VENDEL ◽  
B. STRÖMBECK ◽  
...  
Keyword(s):  
Sister Chromatid ◽  
Chromosome Aberrations ◽  
Sister Chromatid Exchanges ◽  
Structural Chromosome ◽  
Chromatid Exchanges

Distribution of sister chromatid exchanges on the mouse chromosomes in vivo with reference to the replication properties of the X chromosome

1984 ◽  
Vol 26 (2) ◽  
pp. 152-157
Author(s):  
S. M. Singh ◽  
D. L. Reimer
Keyword(s):  
Bone Marrow ◽  
X Chromosome ◽  
Sister Chromatid ◽  
Bone Marrow Cells ◽  
Relative Length ◽  
Chromosome Length ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges ◽  
Marrow Cells

Frequency of sister chromatid exchanges (SCE) were recorded separately for different chromosomes from bone marrow cells of female mice of the two genetic strains (C3H/S and C57BL/6J). SCEs were evaluated following different doses of 5-bromo-2′deoxyuridine (BrdU) as nine hourly i.p. injections. The SCE per cell increased with increasing BrdU doses which was slightly higher in C3H/S than in the C57BL/6J. SCEs per cell were variable at every treatment – strain combination, possibly reflecting the heterogeneous nature of the bone marrow cells. In general, there is a positive correlation between SCE per chromosome and the relative chromosome length. Total SCEs on one of the large chromosomes (most likely the X chromosome), however, are significantly higher than expected on the basis of relative length alone. Most of this increase is attributable to one of the homologues of this chromosome, which is not in synchrony with the rest of the chromosomes and may represent the late-replicating X. These results when viewed in the light of replication properties of the heterochromatinized X, suggest a direct involvement of DNA replication in SCE formation and may argue against the replication point as the sole site for the SCEs.Key words: sister chromatid exchange, BrdU, recombination, replication, X chromosome.

Sign in / Sign up

Export Citation Format

Share Document