Individual and strain differences in patterns of long-term persistence of urethane-induced sister chromatid exchanges (sces) in mouse lymphocytes and their relation to carcinogen susceptibility

1988 ◽  
Vol 12 (4) ◽  
pp. 375-383 ◽  
Author(s):  
Akira Endo ◽  
Toshiaki Watanabe
Keyword(s):  
Sister Chromatid ◽  
Strain Differences ◽  
Sister Chromatid Exchanges ◽  
Chromatid Exchanges ◽  
Mouse Lymphocytes

CYCLOPHOSPHAMIDE INDUCED IN VIVO SISTER CHROMATID EXCHANGES (SCE) IN MUS MUSCULUS. I: STRAIN DIFFERENCES AND EMPIRICAL ASSOCIATION WITH RELATIVE CHROMOSOME SIZE

1982 ◽  
Vol 24 (5) ◽  
pp. 521-528 ◽  
Author(s):  
Dorothy L. Reimer ◽  
Shiva M. Singh
Keyword(s):  
Sister Chromatid ◽  
Bone Marrow Cells ◽  
Strain Differences ◽  
Chromosome Length ◽  
Sister Chromatid Exchanges ◽  
Differential Staining ◽  
Base Line ◽  
Genetic Strains ◽  
Chromatid Exchanges

The inducibility of sister chromatid exchanges (SCEs) by cyclophosphamide (CP) in bone marrow cells was evaluated in vivo in the three genetic strains of mice (C3H/s, C57BL/6J, and Balb/c). Female mice (10 to 12 wks old, mean = 22.9 g, SD = 3.2 g) were administered with nine hourly injections of 214.19 mg/kg 5-Bromo-2′ deoxyuridine (BrdU) followed by 0, 0.048, 0.449, 4.585 or 46.93 mg/kg CP and 4 mg/kg colcemid. SCEs were evaluated following differential staining procedures of Perry and Wolff (1974). The base-line SCEs were similar in all strains with about ten SCEs/cell. Increasing CP concentrations yielded an increased level of SCEs. Most cells showed extensive damage in CP doses exceeding 4.55 mg/kg. No SCE evaluation was possible beyond this concentration. Strain differences were evident at every dose of CP, and Balb/c was the least susceptible strain to SCE induction. F1 hybrids involving C3H/s ♂ and Balb/c ♀ showed SCE values closer to Balb/c. Data on the association between chromosome length and frequency of SCEs are provided. They empirically establish a positive correlation (r = 0.90) between the two features. Most induced SCEs were interstitially located rather than terminally positioned on the chromosome.

scholarly journals Association of Polymorphisms of Phase I Metabolizing Genes with Sister Chromatid Exchanges in Occupational Workers Exposed to Toluene Used in Paint Thinners

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kanu Priya ◽  
Anita Yadav ◽  
Neeraj Kumar ◽  
Sachin Gulati ◽  
Neeraj Aggarwal ◽  
...  
Keyword(s):  
Phase I ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Sister Chromatid Exchanges ◽  
Confounding Factors ◽  
Genetic Damage ◽  
Blood Samples ◽  
Genotoxic Risk ◽  
Chromatid Exchanges

This study investigated genetic damage in paint workers mainly exposed to toluene as it is a major solvent used in paint thinners. Sister chromatid exchange (SCE) assay was used as biomarker of genotoxicity. Blood samples were collected from 30 paint workers and 30 control subjects matched with respect to age and other confounding factors except for exposure to toluene. SCE frequency was found to be significantly higher in paint workers (4.81±0.92) as compared to control individuals (1.73±0.54) (p<0.05). We also investigated influence of polymorphisms of CYP2E1 and CYP1A1m2 genes on SCE frequency. Our results showed that there was significant increase in frequencies of SCE among the mutant genotypes of CYP2E1 and CYP1A1m2 as compared to wild genotypes. Our study indicated that long term exposure of toluene can increase genotoxic risk in paint workers.

scholarly journals CYCLOPHOSPHAMIDE-INDUCED IN VIVO SISTER CHROMATID EXCHANGES (SCE) IN MUS MUSCULUS. III. QUANTITATIVE GENETIC ANALYSIS

Genetics ◽  
1983 ◽  
Vol 105 (1) ◽  
pp. 169-179
Author(s):  
Dorothy L Reimer ◽  
Shiva M Singh
Keyword(s):  
Sister Chromatid ◽  
Major Gene ◽  
Strain Differences ◽  
Diallel Cross ◽  
Sister Chromatid Exchanges ◽  
F1 Hybrids ◽  
Independent Events ◽  
Genetic Strains ◽  
Chromatid Exchanges

ABSTRACT In vivo cyclophosphamide (CP)-induced sister chromatid exchanges (SCEs) were evaluated in females from five genetic strains of mice (C57BL/6J, C3H/S, 129/ReJ, BALB/c and DBA/2) and their F1 hybrids. Baseline (noninduced) SCE values differ significantly among strains, 129/ReJ having the lowest and DBA/2 having the highest mean SCE per cell values. In general, the baseline SCE of a given F1 is within the range of its corresponding parental strains or near the lower parental value. Furthermore, there is a genotype-dependent increase in mean SCEs per cell with CP dose. Strain differences in SCE induction are noted particularly at the two higher CP doses (4.50 and 45.0 mg/kg). In general, F1 hybrids involving a strain with high induced SCEs and a strain with low induced SCEs exhibit mean SCE values that are closer to the value of the lower strain. F1s involving two strains with high SCEs or two strains with low SCEs yield SCEs not different from parental strains. The method of diallel cross analysis showed the order of dominance of these strains in SCE induction to be 129/ReJ BALB/c C3H/S DBA/2 C57BL/6J. These results support the involvement of predominantly nonadditive genetic factors as major gene(s) in SCE induction. In addition, involvement of random and independent events in SCE induction is suggested by the distribution of SCEs which follows a Poisson distribution.

Increased sister chromatid exchanges in epileptic children during long-term therapy with phenytoin

1982 ◽  
Vol 61 (1) ◽  
pp. 71-72 ◽  
Author(s):  
M. Habedank ◽  
K. -J. E�er ◽  
D. Br�ll ◽  
F. Kotlarek ◽  
C. Stumpf
Keyword(s):  
Sister Chromatid ◽  
Sister Chromatid Exchanges ◽  
Term Therapy ◽  
Epileptic Children ◽  
Long Term Therapy ◽  
Chromatid Exchanges
Sign in / Sign up

Export Citation Format

Share Document