scholarly journals Erratum to “Quantitative Fraction Evaluation of Dermal Collagen and Elastic Fibres in the Skin Samples Obtained in Two Orientations from the Trunk Region”

2015 ◽  
Vol 2015 ◽  
pp. 1-1
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Keerthana Prasad ◽  
Ranjini Kudva ◽  
...  
Keyword(s):  
Elastic Fibres ◽  
Trunk Region ◽  
Dermal Collagen ◽  
Skin Samples

scholarly journals Quantitative Fraction Evaluation of Dermal Collagen and Elastic Fibres in the Skin Samples Obtained in Two Orientations from the Trunk Region

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Keerthana Prasad ◽  
Ranjini Kudva ◽  
...  
Keyword(s):  
Image Analysis ◽  
Healing Process ◽  
Wound Healing Process ◽  
Elastic Fibres ◽  
Trunk Region ◽  
Analysis Technique ◽  
Significant Difference ◽  
Dermal Collagen ◽  
Lateral Chest ◽  
Skin Samples

Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications.Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image analysis. Statistical analysis involving mean with SEM and pairedttest by SPSS were employed to the percentage values obtained from image analysis.Result. Among the chosen 5 areas of trunk region, abdomen showed the statistically significant difference for both collagen and elastic content between horizontal and vertical orientations (P< 0.05), whereas upper back, presternal, and lateral chest areas showed significant difference (P< 0.05) only for collagen and groin only for elastic content.Conclusion. The differences in the distribution of dermal collagen and elastic fibres in 2 directions of the samples from the same areas might be attributed to final outcome of wound healing process by influencing the appearance and behaviour of scar related complications in the region of trunk.

scholarly journals Surgical Implications of Asymmetric Distribution of Dermal Collagen and Elastic Fibres in Two Orientations of Skin Samples from Extremities

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Keerthana Prasad ◽  
Ranjini Kudva ◽  
...  
Keyword(s):  
Shoulder Joint ◽  
Histological Section ◽  
The Body ◽  
Asymmetric Distribution ◽  
Elastic Fibres ◽  
Unequal Distribution ◽  
Significant Difference ◽  
Dermal Collagen ◽  
Outcome Result ◽  
Skin Samples

Background. Clinically, scar related complications are observed to be dissimilar in different regions of the body. Unequal distribution of dermal collagen and elastic fibres in different orientations could be one of the multifocal causes of scar related complications, for which this evaluating study has been taken up. Materials and Method. 300 skin samples collected in horizontal and vertical orientations were studied histomorphometrically. This study involved image analysis of specially stained histological section using tissue-quant software. The outcome result was termed as quantitative fraction. From the result, various ratio values were also calculated for the ratio analysis. Results. The differences in the quantitative fraction of dermal elastic content between 2 directions were statistically significant at joint areas (shoulder joint, wrist, and ankle) (P<0.001) but for collagen, significant difference was observed at shoulder joint and wrist only. Dermis of the forearm and thigh did not show any differences in their collagen content, but for elastic, thigh did show a significant difference while forearm had no change between 2 directions. Conclusion. Analysis of unequal content of dermal element in two directions under the perspective of wound healing consequences is subjective depending upon the anatomical position and functional status of the areas.

scholarly journals Collagen and Elastic Fiber Content Correlation Analysis between Horizontal and Vertical Orientations of Skin Samples of Human Body

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Ranjini Kudva ◽  
Sudarshan Surendran ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Negative Correlation ◽  
Anterior Abdominal Wall ◽  
Elastic Fiber ◽  
Elastic Fibers ◽  
Correlation Pattern ◽  
Unequal Distribution ◽  
Dermal Collagen ◽  
Lateral Chest ◽  
Skin Samples

Background. Unequal distribution of dermal collagen and elastic fibers in different orientations of skin is reported to be one of the multifocal causes of scar related complications. Present study is to understand the correlation pattern between collagen in horizontal (CH) and in vertical (CV) directions as well as that of elastic in horizontal (EH) and vertical (EV) directions.Materials and Method. A total of 320 skin samples were collected in two orientations from suprascapular, anterior chest, lateral chest, anterior abdominal wall, and inguinal regions of 32 human cadavers. Spearman correlation coefficient (r) was calculated between the variables (CH,CV,EH, andEV).Results. Significant positive correlation betweenCHandCV, and betweenEHandEVobserved in all 5 areas tested. A negative correlation betweenCVandEVat suprascapular, lateral chest, and inguinal regions and negative correlation betweenCHandEHat anterior chest and anterior abdominal wall have been identified.Conclusion. Knowledge of asymmetric content of dermal collagen and elastic fibers together with the varied strength and degree of association in the given area provides guidelines to the dermatologists and aesthetic surgeons in placing elective incisions in the direction maximally utilizing the anatomical facts for aesthetically pleasing result.

Effects of Experimentally Induced Fleece-rot on the Structure of the Skin of Merino Sheep

1982 ◽  
Vol 35 (5) ◽  
pp. 545 ◽  
Author(s):  
DE Hollis ◽  
RE Chapman ◽  
JA Hemsley
Keyword(s):  
Histological Examination ◽  
Merino Sheep ◽  
Trunk Region ◽  
Simulated Rain ◽  
Skin Samples ◽  
Experimentally Induced

Four medium- and six strong-wool Merino sheep were exposed to continuous simulated rain (4'5 mm/h) for periods ranging from 3 to 9 days. In response, the sheep developed characteristics typical of fleece-rot lesions such as exudative encrustation' and bacterial discoloration of the fleece. Skin samples were taken periodically for histological examination from the trunk region of the sheep prior to, during, and after wetting.

scholarly journals The Rapid Inactivation of Porcine Skin by Applying High Hydrostatic Pressure without Damaging the Extracellular Matrix

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Naoki Morimoto ◽  
Atsushi Mahara ◽  
Kouji Shima ◽  
Mami Ogawa ◽  
Chizuru Jinno ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Saline Solution ◽  
Cultured Cells ◽  
Mitochondrial Activity ◽  
Porcine Skin ◽  
Dermal Matrix ◽  
Rapid Inactivation ◽  
Dermal Collagen ◽  
Skin Samples

We previously reported that high hydrostatic pressure (HHP) of 200 MPa for 10 minutes could induce cell killing. In this study, we explored whether HHP at 200 MPa or HHP at lower pressure, in combination with hyposmotic distilled water (DW), could inactivate the skin, as well as cultured cells. We investigated the inactivation of porcine skin samples 4 mm in diameter. They were immersed in either a normal saline solution (NSS) or DW, and then were pressurized at 100 and 200 MPa for 5, 10, 30, or 60 min. Next, we explored the inactivation of specimens punched out from the pressurized skin 10 × 2 cm in size. The viability was evaluated using a WST-8 assay and an outgrowth culture. The histology of specimens was analyzed histologically. The mitochondrial activity was inactivated after the pressurization at 200 MPa in both experiments, and no outgrowth was observed after the pressurization at 200 MPa. The arrangement and proportion of the dermal collagen fibers or the elastin fibers were not adversely affected after the pressurization at 200 MPa for up to 60 minutes. This study showed that a HHP at 200 MPa for 10 min could inactivate the skin without damaging the dermal matrix.

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

ESTIMATION OF EFFICIENCY OF COLLAGEN AND BIOLOGICAL GLUE AT DUODENAL FISTULA IN EXPERIMENT

2018 ◽  
Vol 8 (1) ◽  
pp. 69-75
Author(s):  
R. R. Ishtukov ◽  
M. A. Nartailakov ◽  
V. S. Panteleev ◽  
V. V. Rezyapov
Keyword(s):  
Intestinal Tract ◽  
Histological Study ◽  
Abdominal Cavity ◽  
Suture Line ◽  
Intestinal Fistula ◽  
Duodenal Fistula ◽  
Morphological Studies ◽  
Vascular Congestion ◽  
Dermal Collagen ◽  
Surgical Tactics

Introduction. One of the worst complication of different surgical diseases of abdominal cavity organs and of performed, on that regard, operational interventions, are intestinal festulas including without limitation deodenum fistulas. Longterm experience of surgical treatment of patients with unformed duodenal and high enteric fistulas show that it is feasible to have a clear surgical tactics with account of location level and type of fistulas, their quantity, volume of sarcous reduction, peritonitis and multi-organ failure syndrome. In this regard, the objective of this research is to find optimal surgical tactics and to determine the most effective method of treating duodenum fistulas of various causation.Materials and methods. Experimental work was carried out under conditions of experimentally formed intestinal fistula in rabbits of “Shinshilla” breed. After formation of fistula the animals were divided into 3 groups based on the method of removing artificially installed duodenal fistula: sealing through Albert — Schmieden — Lembert suture without fastening of suture line; sealing through fastening of intestinal suture with biological surgical glue; sealing through fastening with swine dermal collagen. Mechanical constancy of the sutures was measured with pneumocompression of sealed intestinal tract areas as well as with morphological study of surgical wound edges.Results. The results of histological study show that experiments with application of bio-implant demonstrated less vascular congestion and interstitial swelling. Phologistic infiltration also responded more efficiently both quantitatively (manifestation rate) and qualitatively (quick change of cell elements) in the group that used bio-implant.Conclusion. Results of morphological studies and pneumocompression data under modelling of duodenal fistulas in experimental animals show that application of bio-implant helps to earlier restore microcirculatory abnormality. 

The Estimation of dermal collagen in osteoporotic patients by a histomorphometric method*

Pathology ◽  
1978 ◽  
Vol 10 (4) ◽  
pp. 365-371 ◽  
Author(s):  
A.S.Y. Leong ◽  
P. Balasubramaniam
Keyword(s):  
Dermal Collagen
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