scholarly journals Collagen and Elastic Fiber Content Correlation Analysis between Horizontal and Vertical Orientations of Skin Samples of Human Body

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Ranjini Kudva ◽  
Sudarshan Surendran ◽  
...  
Keyword(s):  
Abdominal Wall ◽  
Negative Correlation ◽  
Anterior Abdominal Wall ◽  
Elastic Fiber ◽  
Elastic Fibers ◽  
Correlation Pattern ◽  
Unequal Distribution ◽  
Dermal Collagen ◽  
Lateral Chest ◽  
Skin Samples

Background. Unequal distribution of dermal collagen and elastic fibers in different orientations of skin is reported to be one of the multifocal causes of scar related complications. Present study is to understand the correlation pattern between collagen in horizontal (CH) and in vertical (CV) directions as well as that of elastic in horizontal (EH) and vertical (EV) directions.Materials and Method. A total of 320 skin samples were collected in two orientations from suprascapular, anterior chest, lateral chest, anterior abdominal wall, and inguinal regions of 32 human cadavers. Spearman correlation coefficient (r) was calculated between the variables (CH,CV,EH, andEV).Results. Significant positive correlation betweenCHandCV, and betweenEHandEVobserved in all 5 areas tested. A negative correlation betweenCVandEVat suprascapular, lateral chest, and inguinal regions and negative correlation betweenCHandEHat anterior chest and anterior abdominal wall have been identified.Conclusion. Knowledge of asymmetric content of dermal collagen and elastic fibers together with the varied strength and degree of association in the given area provides guidelines to the dermatologists and aesthetic surgeons in placing elective incisions in the direction maximally utilizing the anatomical facts for aesthetically pleasing result.

scholarly journals Surgical Implications of Asymmetric Distribution of Dermal Collagen and Elastic Fibres in Two Orientations of Skin Samples from Extremities

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Keerthana Prasad ◽  
Ranjini Kudva ◽  
...  
Keyword(s):  
Shoulder Joint ◽  
Histological Section ◽  
The Body ◽  
Asymmetric Distribution ◽  
Elastic Fibres ◽  
Unequal Distribution ◽  
Significant Difference ◽  
Dermal Collagen ◽  
Outcome Result ◽  
Skin Samples

Background. Clinically, scar related complications are observed to be dissimilar in different regions of the body. Unequal distribution of dermal collagen and elastic fibres in different orientations could be one of the multifocal causes of scar related complications, for which this evaluating study has been taken up. Materials and Method. 300 skin samples collected in horizontal and vertical orientations were studied histomorphometrically. This study involved image analysis of specially stained histological section using tissue-quant software. The outcome result was termed as quantitative fraction. From the result, various ratio values were also calculated for the ratio analysis. Results. The differences in the quantitative fraction of dermal elastic content between 2 directions were statistically significant at joint areas (shoulder joint, wrist, and ankle) (P<0.001) but for collagen, significant difference was observed at shoulder joint and wrist only. Dermis of the forearm and thigh did not show any differences in their collagen content, but for elastic, thigh did show a significant difference while forearm had no change between 2 directions. Conclusion. Analysis of unequal content of dermal element in two directions under the perspective of wound healing consequences is subjective depending upon the anatomical position and functional status of the areas.

Elastic fibers in the anterior abdominal wall

Hernia ◽  
2011 ◽  
Vol 15 (4) ◽  
pp. 409-415 ◽  
Author(s):  
A. Fachinelli ◽  
M. R. M. Trindade ◽  
F. A. Fachinelli
Keyword(s):  
Abdominal Wall ◽  
Anterior Abdominal Wall ◽  
Elastic Fibers

scholarly journals Quantitative Fraction Evaluation of Dermal Collagen and Elastic Fibres in the Skin Samples Obtained in Two Orientations from the Trunk Region

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Keerthana Prasad ◽  
Ranjini Kudva ◽  
...  
Keyword(s):  
Image Analysis ◽  
Healing Process ◽  
Wound Healing Process ◽  
Elastic Fibres ◽  
Trunk Region ◽  
Analysis Technique ◽  
Significant Difference ◽  
Dermal Collagen ◽  
Lateral Chest ◽  
Skin Samples

Background. Histomorphic evaluation of dermal collagen and elastic fibres was analysed by image analysis technique. The quantification of dermal elements was performed in skin tissues, collected in horizontal and vertical directions from trunk region and discussed under the perspective of consequences of scar related complications.Materials and Method. Total number of 200 skin samples collected from 5 areas of trunk region were processed histologically and subjected to tissue-quant image analysis. Statistical analysis involving mean with SEM and pairedttest by SPSS were employed to the percentage values obtained from image analysis.Result. Among the chosen 5 areas of trunk region, abdomen showed the statistically significant difference for both collagen and elastic content between horizontal and vertical orientations (P< 0.05), whereas upper back, presternal, and lateral chest areas showed significant difference (P< 0.05) only for collagen and groin only for elastic content.Conclusion. The differences in the distribution of dermal collagen and elastic fibres in 2 directions of the samples from the same areas might be attributed to final outcome of wound healing process by influencing the appearance and behaviour of scar related complications in the region of trunk.

scholarly journals Determination of Spearman Correlation Coefficient(r)to Evaluate the Linear Association of Dermal Collagen and Elastic Fibers in the Perspectives of Skin Injury

2018 ◽  
Vol 2018 ◽  
pp. 1-6
Author(s):  
Naveen Kumar ◽  
Pramod Kumar ◽  
Satheesha Nayak Badagabettu ◽  
Melissa Glenda Lewis ◽  
Murali Adiga ◽  
...  
Keyword(s):  
Correlation Coefficients ◽  
Scar Formation ◽  
Elastic Fibers ◽  
Strong Positive Correlation ◽  
Spearman Correlation ◽  
Unequal Distribution ◽  
Positive Correlation ◽  
Dermal Collagen ◽  
Human Cadavers ◽  
Moderate Negative Correlation

Background. Difference in scar formation at different sites, in different directions at the same site, but with changes in the elasticity of skin with age, sex, and race or in some pathological conditions, is well known to clinicians. The inappropriate collagen syntheses and delayed or lack of epithelialization are known to induce scar formation with negligible elasticity at the site of damage. Changes in the elasticity of scars may be due to an unequal distribution of dermal collagen (C) and elastic (E) fibers.Materials and Methods. Spearman correlation coefficients (r) of collagen and elastic fibers in horizontal (H) and in vertical (V) directions (variables CV, CH, EV, and EH) were measured from the respective quantitative fraction data in 320 skin samples from 32 human cadavers collected at five selected sites over extremities.Results. Spearman’s correlation analysis revealed the statistically significant (p<0.01) strong positive correlation betweenCHandCVin all the areas, that is, shoulder joint area (r=0.66), wrist (r=0.75), forearm (r=0.75), and thigh (r=0.80), except at the ankle (r=0.26,p=0.14) region. Similarly, positive correlation betweenEHandEVhas been observed at the forearm (r=0.65, moderate) and thigh (r=0.42, low) regions. However, a significant moderate negative correlation was observed betweenCVandEVat the forearm (r=-0.51) and betweenCHandEHat the thigh region (r=-0.65).Conclusion. Significant differences of correlations of collagen and elastic fibers in different directions from different areas of extremities were noted. This may be one of the possible anatomical reasons of scar behavior in different areas and different directions of the same area.

Conventional Nanofat and SVF/ADSC-Concentrated Nanofat: A Comparative Study on Improving Photoaging of Nude Mice Skin

2019 ◽  
Vol 39 (11) ◽  
pp. 1241-1250 ◽  
Author(s):  
Hui Zheng ◽  
Lihong Qiu ◽  
Yingjun Su ◽  
Chenggang Yi
Keyword(s):  
Nude Mice ◽  
Lower Layer ◽  
Elastic Fiber ◽  
Stromal Vascular Fraction ◽  
Collagen Content ◽  
Elastic Fibers ◽  
Saline Injection ◽  
Fiber Morphology ◽  
Dermal Thickness ◽  
Dermal Collagen

AbstractBackgroundNanofats could improve photoaging. Stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) may play pivotal roles. However, SVFs and ADSCs in nanofats processed by conventional methods cannot be enriched. Some researchers have found that after centrifugation, the SVF/ADSC density increases from top to bottom.ObjectivesThe authors hypothesized that centrifugation can be used to obtain SVF/ADSC-concentrated nanofats that are superior to conventional nanofats in improving the photoaging of skin.MethodsAfter a photoaging model was successfully established in nude mice, the back of each mouse was divided into 4 areas and randomly injected with conventional nanofat, centrifuged nanofat (either the middle or lower layer of centrifuged nanofat), or normal saline. Wrinkles, dermis thickness, dermal collagen content, and elastic fiber morphology were measured and compared at weeks 4 and 8.ResultsCompared with the wrinkles in the physiological saline injection areas, the wrinkles in the areas injected with the 3 nanofats (lower and middle layers of centrifuged nanofat and conventional nanofat) were significantly reduced. All 3 nanofat groups showed increased dermal thickness, increased collagen content, and a more regular distribution of elastic fibers compared with the saline injection areas.ConclusionsThe study established the efficacy of nanofats in improving photoaging by reducing wrinkles and increasing the thickness of dermal collagen, making nanofats a promising novel treatment for photoaging. The SVF/ADSC-concentrated nanofats exhibited the most improvement.

scholarly journals PECULIARITIES OF MORPHOLOGICAL CHANGES OF TISSUES AFTER HERNOPLASTIC DURING RECURRENCE OF POSTOPERATIVE VENTRAL HERNIAS

2019 ◽  
Vol 18 (4) ◽  
pp. 100-106
Author(s):  
V. I. Pyatnochka ◽  
I. Ya. Dzyubanovsky ◽  
A. M. Prodan ◽  
T. V. Datsko
Keyword(s):  
Postoperative Complications ◽  
Connective Tissue ◽  
Abdominal Wall ◽  
Ventral Hernia ◽  
Anterior Abdominal Wall ◽  
Morphological Changes ◽  
Surrounding Tissue ◽  
Elastic Fibers ◽  
Immune Inflammation ◽  
Ventral Hernias

Considering the results of surgical treatment of postoperative ventral hernias, a significant number of relapses, comprising 4.3-46 %, should be noted, and for large and giant postoperative ventral hernias reaches 80 %. The lack of clear criteria for assessing the local response of the tissues of the anterior abdominal wall to the implantation of various types of mesh implants and the associated early wound postoperative complications and relapses prompts further study of the morphological features of the anterior abdominal wall tissue responses in patients with primary and postoperative ventral hernias. Aim of the study: to establish morphological patterns of the restructuring of the tissues of the anterior abdominal wall in patients with recurrent ventral hernia. Material and methods. An in-depth comprehensive clinical, instrumental and laboratory examination of 1419 patients with primary and postoperative ventral hernia was performed. There were 250 patients with recurrent ventral hernia (17.62 %). Results and conclusions. The use of a “light mesh” in patients with recurrent postoperative ventral hernia in the presence of concomitant NDCT appears to be significantly less lymphohistiocytic and leukocyte infiltration for the surrounding tissue, and reduces the likelihood of postoperative complications. Morphological changes in the tissue of the anterior abdominal wall with NDCT strongly indicate a disorder of its architectonics. It is clinically reflected in the formation of postoperative and recurrent hernias. Separate muscle fibers lose cross striation. In the muscular aponeurotic component without signs of connective tissue dysplasia, the application of various types of nets leads to the formation of elastic and collagen fibers, with minor changes in their architectonics and minimal cell infiltration of the immune inflammation of the extracellular matrix. Disintegration, destructive-dystrophic changes in the architectonics of the connective tissue were observed in patients with signs of NDCT in relapses. When using the “heavy” mesh, significant disorientation, collagenolysis, reduced synthesis of all types of collagens, the phenomenon of thickening of elastic fibers and elastolysis were observed. Inflammatory infiltration, triggered by immune inflammation cells, increased mucoid and fibrinoid edema, which led to homogenization, local lysis, and focal tissue destruction.

Determination of clinical efficiency of deproteinized dialysate from blood of milk-fed calves after endoprosthetics in patients with ventral hernias

2020 ◽  
Vol 28 (3) ◽  
pp. 323-333
Author(s):  
Sergej V. Ivanov ◽  
Ilya S. Ivanov ◽  
Evgenij G. Obyedkov ◽  
Liliya P. Popova
Keyword(s):  
Abdominal Wall ◽  
Inflammatory Reaction ◽  
Inflammatory Process ◽  
Anterior Abdominal Wall ◽  
Dynamic Change ◽  
Main Group ◽  
Control Group ◽  
Surgical Department ◽  
Regeneration Stage

Aim. To study the influence of deproteinized dialysate from blood of milk-fed calves on the type of exudative discharge and dynamics of inflammatory reaction after hernia repair with plastics of the anterior abdominal wall with hernioendoprosthesis of polypropylene. Materials and Methods. The study involved 59 patients being on stationary treatment in the surgical department of Kursk Regional Clinical Hospital. Patients were hospitalized for the herniation of small or medium dimension. The patients were divided to two groups: the main (n=30) and control (n=29) groups. After endoprosthetics, the patients of the control group were given complex conservative treatment. The patients of the main group, besides standard treatment, were administered deproteinized dialysate from blood of milk-fed calves intravenously in drips 10 ml + 200 ml of 0.9% sodium chloride solution within 7 days. For cytological examination and determination of the type of cytograms, the traumatic discharge was collected and analyzed using the method of M.F. Kamaev and M.A. Palthsev. Results. Cytomorphometric examination was conducted on the third, fifth and seventh day after endoprosthetics with the aim of studying dynamic changes. Determination of cell composition and also of its changes characteristic of each studied period, was necessary for obtaining further information characterizing inflammatory process in the region of placement of the endoprosthesis. After endoprosthetics in patients who were administered hemodialysate, the inflammatory reaction was less pronounced than in patients who did not receive the preparation. This was associated with a more dynamic change of stages of the inflammatory process. In patients of the main group who received deproteinized dialysate, regenerative type of inflammation first appeared on the fifth day and made 6.9%; by the seventh day the share of patients with the regenerative type rose to 17.5%, while in the patients of the control group no regeneration stage was observed in both periods. Conclusion. Analysis of the efficiency of influence of hemodialysate on the inflammatory reaction in plastics of the anterior abdominal wall with polypropylene endoprosthesis evidences faster course of all stages of inflammation and reduction of its intensity by 10%.

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