scholarly journals Modulation of the Cardiomyocyte Contraction inside a Hydrostatic Pressure Bioreactor:In VitroVerification of the Frank-Starling Law

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Lorenzo Fassina ◽  
Giovanni Magenes ◽  
Roberto Gimmelli ◽  
Fabio Naro
Keyword(s):  
Image Processing ◽  
Hydrostatic Pressure ◽  
Functional Classification ◽  
Video Registration ◽  
Chronotropic Effects

We have studied beating mouse cardiac syncytiain vitroin order to assess the inotropic, ergotropic, and chronotropic effects of both increasing and decreasing hydrostatic pressures. In particular, we have performed an image processing analysis to evaluate the kinematics and the dynamics of those pressure-loaded beating syncytia starting from the video registration of their contraction movement. By this analysis, we have verified the Frank-Starling law of the heart inin vitrobeating cardiac syncytia and we have obtained their geometrical-functional classification.

scholarly journals Model of Murine Ventricular Cardiac Tissue for In Vitro Kinematic-Dynamic Studies of Electromagnetic and β-Adrenergic Stimulation

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Lorenzo Fassina ◽  
Marisa Cornacchione ◽  
Manuela Pellegrini ◽  
Maria Evelina Mognaschi ◽  
Roberto Gimmelli ◽  
...  
Keyword(s):  
Image Processing ◽  
Cardiac Tissue ◽  
Stimulation Frequency ◽  
Adrenergic Stimulation ◽  
Inotropic Effects ◽  
Electromagnetic Stimulation ◽  
Video Registration ◽  
Dynamic Studies

In a model of murine ventricular cardiac tissue in vitro, we have studied the inotropic effects of electromagnetic stimulation (frequency, 75 Hz), isoproterenol administration (10 μM), and their combination. In particular, we have performed an image processing analysis to evaluate the kinematics and the dynamics of beating cardiac syncytia starting from the video registration of their contraction movement. We have found that the electromagnetic stimulation is able to counteract the β-adrenergic effect of isoproterenol and to elicit an antihypertrophic response.

Specific Labeling of Protein Domains with Antibody Fragments

1981 ◽  
Vol 39 ◽  
pp. 416-417
Author(s):  
U. Aebi ◽  
L.E. Buhle ◽  
W.E. Fowler
Keyword(s):  
Image Processing ◽  
Specific Protein ◽  
Antibody Fragments ◽  
Supramolecular Organization ◽  
Two Dimensional ◽  
Ordered Structures ◽  
Virus Capsids ◽  
Processing Techniques

Many important supramolecular structures such as filaments, microtubules, virus capsids and certain membrane proteins and bacterial cell walls exist as ordered polymers or two-dimensional crystalline arrays in vivo. In several instances it has been possible to induce soluble proteins to form ordered polymers or two-dimensional crystalline arrays in vitro. In both cases a combination of electron microscopy of negatively stained specimens with analog or digital image processing techniques has proven extremely useful for elucidating the molecular and supramolecular organization of the constituent proteins. However from the reconstructed stain exclusion patterns it is often difficult to identify distinct stain excluding regions with specific protein subunits. To this end it has been demonstrated that in some cases this ambiguity can be resolved by a combination of stoichiometric labeling of the ordered structures with subunit-specific antibody fragments (e.g. Fab) and image processing of the electron micrographs recorded from labeled and unlabeled structures.

Effects of hydrostatic pressure and inert gases on platelet aggregation in vitro

1990 ◽  
Vol 69 (6) ◽  
pp. 2239-2247 ◽  
Author(s):  
D. M. Pickles ◽  
D. Ogston ◽  
A. G. Macdonald
Keyword(s):  
Platelet Aggregation ◽  
Hydrostatic Pressure ◽  
Maximum Degree ◽  
Platelet Rich Plasma ◽  
Control Level ◽  
Inert Gases ◽  
Partial Pressures ◽  
Compression Cycle ◽  
Anesthetic Potency

A novel cuvette was used to subject citrated platelet-rich plasma (PRP) to high hydrostatic pressure with negligible contamination by He (used for compression of the apparatus). Aggregation was induced at pressure by ADP and quantified turbidimetrically. The maximum degree of aggregation (MDA) was reduced from a control level of 82.2 to 53.6% by exposure to 101 ATA. Because decompression bubbles did not form, aggregation was also measured immediately after a compression cycle. After exposure to 101 ATA hydrostatic pressure, platelets responded normally to ADP at 1 ATA. In a matching apparatus, PRP was equilibrated with high partial pressures of inert gases. Normal physiological plasma Po2 and pH were maintained during equilibration. N2O (5 ATA) reduced the MDA from 86.5 (control) to 58.1%. N2 (51 ATA) reduced the MDA from 74.7 (control) to 51.6%, and 101 ATA Pn2 reduced the MDA from 78.0 (control) to 32.3%. He (100 ATA) reduced the MDA from 83.6 to 38.6%. It was concluded that platelet aggregation was relatively sensitive to hydrostatic pressure and less sensitive to inert gases than predicted from their anesthetic potency ratios.

Cyclic hydrostatic pressure stimulates enhanced bone development in the foetal chick femur in vitro

Bone ◽  
2013 ◽  
Vol 53 (2) ◽  
pp. 468-477 ◽  
Author(s):  
J.R. Henstock ◽  
M. Rotherham ◽  
J.B. Rose ◽  
A.J. El Haj
Keyword(s):  
Hydrostatic Pressure ◽  
Bone Development

scholarly journals High hydrostatic pressure: a new way to improve in vitro developmental competence of porcine matured oocytes after vitrification

Reproduction ◽  
2007 ◽  
Vol 135 (1) ◽  
pp. 13-17 ◽  
Author(s):  
Y Du ◽  
C S Pribenszky ◽  
M Molnar ◽  
X Zhang ◽  
H Yang ◽  
...  
Keyword(s):  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Developmental Competence

Effects of Chondroitin Sulphate on Fluid and Solute Transport during Peritoneal Dialysis in Rats

1991 ◽  
Vol 11 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Andrzej Breborowicz ◽  
Maciej Radkowski ◽  
Jan Knapowski ◽  
Dimitrios G. Oreopoulos
Keyword(s):  
Peritoneal Dialysis ◽  
Hydrostatic Pressure ◽  
Hydraulic Conductivity ◽  
Horseradish Peroxidase ◽  
Solute Transport ◽  
Peritoneal Cavity ◽  
Chondroitin Sulphate ◽  
In Vitro Experiments ◽  
Fluid Removal

The effect of chondroitin sulphate (CS) on peritoneal fluid and solute transport was studied in rats undergoing peritoneal dialysis. In the presence of CS, net ultrafiltration increased, while absorption of glucose and horseradish peroxidase from the peritoneal cavity decreased. Albumin, used instead of CS, did not modify either fluid or solute transport. In in vitro experiments on isolated rabbit mesentery, CS decreased transmembrane water flow induced by hydrostatic pressure, and its effect was not fully reversed 60 minutes after “wash-out” of this glycosaminoglycan. We postulate that the polyanionic CS molecules are trapped in the peritoneal interstitium, thus decreasing its hydraulic conductivity and permeability, which in turn increases net fluid removal during peritoneal dialy sis because of its slower absorption from the peritoneal cavity.

Sign in / Sign up

Export Citation Format

Share Document