scholarly journals Cultivated Oral Mucosa Epithelium in Ocular Surface Reconstruction in Aniridia Patients

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Dariusz Dobrowolski ◽  
Boguslawa Orzechowska-Wylegala ◽  
Bogumil Wowra ◽  
Ewa Wroblewska-Czajka ◽  
Maria Grolik ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Oral Mucosa ◽  
Amniotic Membrane ◽  
Single Cells ◽  
Autologous Transplantation ◽  
Visual Axis ◽  
Corneal Surface ◽  
Ocular Surface Reconstruction ◽  
3T3 Fibroblasts ◽  
Mucosa Epithelium

Purpose. Efficacy of cultivated oral mucosa epithelial transplantation (COMET) procedure in corneal epithelium restoration of aniridia patients.Methods. Study subjects were aniridia patients (13 patients; 17 eyes) with irregular, vascular conjunctival pannus involving visual axis who underwent autologous transplantation of cultivated epithelium. For the procedure oral mucosa epithelial cells were obtained from buccal mucosa with further enzymatic treatment. Suspension of single cells was seeded on previously prepared denuded amniotic membrane. Cultures were carried on culture dishes inserts in the presence of the inactivated with Mitomycin C monolayer of 3T3 fibroblasts. Cultures were carried for seven days. Stratified oral mucosa epithelium with its amniotic membrane carrier was transplanted on the surgically denuded corneal surface of aniridia patients with total or subtotal limbal stem cell deficiency.Outcome Measures. Corneal surface, epithelial regularity, and visual acuity improvement were evaluated.Results. At the end of the observation period, 76.4% of the eyes had regular transparent epithelium and 23.5% had developed epithelial defects or central corneal haze; in 88.2% of cases visual acuity had increased. VA range was from HM 0.05 before the surgery to HM up to 0.1 after surgery.Conclusion. Application of cultivated oral mucosa epithelium restores regular epithelium on the corneal surface with moderate improvement in quality of vision.

scholarly journals Case series of cultivated oral mucosa epithelium transplantation for limbal stem cell failure

2018 ◽  
Vol 16 (2) ◽  
pp. 110-118
Author(s):  
Kah Lay Oh ◽  
Aida Zahidin ◽  
Umi Kalthum Md Noh ◽  
Safinaz Mohd Khialdin ◽  
Ghee Chien Ooi ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Stem Cell ◽  
Oral Mucosa ◽  
Ocular Surface ◽  
Case Series ◽  
Limbal Stem Cell Deficiency ◽  
Corneal Transplant ◽  
Ocular Rosacea ◽  
Limbal Stem Cell ◽  
Mucosa Epithelium

Purpose: To report a case series of seven eyes (six patients) with severe limbal stem cell failure who underwent cultivated oral mucosa epithelium transplantation (COMET) in preparation for corneal transplant surgery and assess the change in visual acuity postoperatively. Design: Case series. Methods: A retrospective analysis of an interventional case series of six patients with severe limbal stem cell deficiency who underwent COMET between 2012 and 2015 was performed. Six eyes had COMET followed by corneal transplant, and one eye had corneal transplant preceding COMET. The change in the visual acuity after operation was evaluated. Results: A total of six patients with seven eyes were studied. All of them had severe ocular surface disease with limbal stem cell deficiency (LSCD) secondary to chemical injury (five eyes), SJS (one eye), and severe ocular rosacea (one eye). Four eyes had improvements in visual acuity, two of which achieved best corrected visual acuity (BCVA) of 6/12. Conclusion: The use of COMET helps to optimize the ocular surface in severe LSCD for further corneal transplant surgeries. It has helped to improve the graft uptake and subsequent visual improvement, especially in chronic chemical burn cases.  

scholarly journals Avtologni veznično-limbalni presadek pri enostranskem popolnem pomanjkanju limbalnih epitelnih matičnih celic

2017 ◽  
Vol 86 (7-8) ◽  
Author(s):  
Petra Schollmayer ◽  
Zala Lužnik
Keyword(s):  
Stem Cells ◽  
Surgical Technique ◽  
Corneal Epithelium ◽  
Ocular Surface ◽  
Autologous Transplantation ◽  
Corneal Transplantation ◽  
Epithelial Stem Cells ◽  
Corneal Surface ◽  
Limbal Transplantation

Background: Corneal epithelium is renewed by stem cells (SC) that reside at the corneal limbus. Reduced number of SC or their abnormal function lead to the ocular surface disease called limbal stem cell deficiency (LSCD), characterized by corneal conjunctivalization, vascularization, persistent epithelial defects, chronic inflammation, and loss of vision. In a case of total unilateral LSCD, autologous transplantation of limbal epithelial stem cells (LESC) from the healthy eye is needed. We describe the surgical technique of choice for autologous limbal transplantation, called conjunctival limbal autograft (CLAU) that we combined with amniotic membrane (AM) use. We present the results of CLAU in three patients with total unilateral LSCD due to chemical injury.Methods: Autologous limbal transplantation CLAU begins with the removal of fibrovascular pannus from the diseased corneal surface and the harvesting of two conjunctival-limbal grafts from the healthy eye. The grafts are then transplanted on to the limbal area of the recipient eye. AM is used as a patch to cover the denuded cornea and limbal grafts, as well as a barrier preventing the conjunctival epithelium from encroaching on to the temporal and nasal side of the corneal surface. In the donor eye, AM is used to cover the donor sites. CLAU with the use of AM was performed in 3 patients with unilateral LSCD due to chemical eye injury. In one patient limbal transplantation was combined with symblepharon lysis for entropium repair. In all cases AM was removed 3–6 days postoperatively to assess the growth of new epithelium from the limbal grafts. In all patients the ocular surface was covered with another AM until the cornea was completely epithelized and the new epithelium stable. In one patient the corneal regrafting and cataract removal was performed subsequently.Results: CLAU was successful in 2 patients and partially successful in 1 patient during the follow up. In all cases the growth of new epithelium from the limbal grafts was noted on day 3–6 after CLAU. The cornea was completely epithelized within 2 weeks in 2 patients and after 35 days in one patient. In two patients the corneal epithelium remained clear, smooth and stable during the follow up of 3.5 years and 4 months, respectively. In one patient, uneven epithelium probably representing a mosaic of corneal and conjunctival cells was noted in the central corneal region, where a small corneal ulcer developed 5 months after CLAU. In donor eyes no postoperative complications were noted, the donor sites epithelized within few days.Conclusions: Autologous limbal transplantation according to CLAU surgical technique combined with the use of AM is a successful and safe therapy for restoring corneal surface in total unilateral LSCD after chemical injury. It enables further surgical procedures for restoring the vision such as corneal transplantation and cataract surgery.

scholarly journals NON-PRESERVED HUMAN AMNIOTIC MEMBRANE TRANSPLANT IN OCULAR SURFACE RECONSTRUCTION- A SURGICAL EXPOSURE IN WESTERN ODISHA

2018 ◽  
Vol 5 (13) ◽  
pp. 1146-1150
Author(s):  
Jagadish Prasad Rout ◽  
Pramod Kumar Sharma ◽  
Swati Samikshya ◽  
Ravindra Kumar Chowdhury ◽  
Kanhei Charan Tudu
Keyword(s):  
Surface Reconstruction ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Human Amniotic Membrane ◽  
Ocular Surface Reconstruction ◽  
Surgical Exposure

scholarly journals THE USE OF INTRAVENOUS LASER BLOOD IRRADIATION IN THE TREATMENT OF PATIENTS WITH BEHCET’S SYNDROME

2019 ◽  
Vol 22 (3-4) ◽  
pp. 104-110
Author(s):  
D. F Porsokhonova ◽  
V. S Novoselov ◽  
A. I Yakubovich ◽  
M. A Mirsaidova ◽  
Yu. N Radzhabova ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Laser Irradiation ◽  
Oral Mucosa ◽  
Clinical Picture ◽  
Lateral Surface ◽  
No Reduction ◽  
External Genitalia ◽  
Complex Therapy ◽  
Blood Irradiation ◽  
Laser Blood Irradiation

The results of a microbiological and PCR survey of patients with Behcet’s disease, as well as the results of intravenous laser irradiation of the blood (ILIB) are presented. According to the authors, the clinical picture of the disease was represented by the presence of single and multiple repeatedly recurring erosive and ulcerative defects in the area of the oral mucosa (cheek area and lateral surface of the tongue) and/or external genitalia. 4 patients noted soreness in the area of rashes, in 2 rashes were accompanied by a rise in temperature and the symptoms of malaise, weakness. Signs of catarrhal conjunctivitis and uveitis were present in 3 patients, while there was no reduction in visual acuity in the course of the disease. 3 patients had pain in large joints without visible changes on the radiograph and an increase in ESR up to 18-24 mm/h. As a result of the complex therapy with antibacterial, antiprotozoal, antiviral, antifungal therapy depending on the detected pathogen, as well as procedures of ILIB, the resorption of the infiltration zone was noted on the 10-11th day of treatment. Ulcerative elements began to epithelize. Erythema, feelings of burning and pain were absent.

The first normal oral mucosa epithelium in which ?-glutamyl transpeptidase activity has been detected

1992 ◽  
Vol 24 (12) ◽  
pp. 964-968 ◽  
Author(s):  
A. E. Schwint ◽  
A. M. Collet ◽  
A. E. Mendez ◽  
R. L. Cabrini ◽  
M. E. Itoiz
Keyword(s):  
Oral Mucosa ◽  
Normal Oral Mucosa ◽  
Glutamyl Transpeptidase ◽  
Mucosa Epithelium

Human amniotic membrane for failed macular hole. A case of initial unsuccessful outcome that resolved after amniotic membrane exchange

2021 ◽  
pp. 112067212110589
Author(s):  
Tomaso Caporossi ◽  
Lorenzo Governatori ◽  
Tommaso Verdina ◽  
Stanislao Rizzo
Keyword(s):  
Visual Acuity ◽  
Macular Hole ◽  
Amniotic Membrane ◽  
Sulfur Hexafluoride ◽  
Silicone Oil ◽  
Human Amniotic Membrane ◽  
Second Surgery ◽  
Myopic Macular Hole ◽  
Oil Injection ◽  
Membrane Patch

Introduction We described a case of initial unsuccessful outcome of failed macular hole treated with amniotic membrane, that resolved after amniotic membrane exchange and silicone oil injection. Case Description a woman affected by a high myopic macular hole that had failed to close after multiple surgeries, had been treated using an amniotic membrane graft and 20% sulfur hexafluoride but, after the gas reabsorption, the macular hole was still open, and the amniotic membrane. A second surgery with a second amniotic membrane patch and silicone oil was carried out. Two weeks after the procedure the macular hole closed, and the final visual acuity improved from 20/400 to 20/100. Conclusions In this case, a second surgery, using a new amniotic membrane graft and a longer-lasting endotamponade, closed the hole and improved the visual acuity.

scholarly journals Kissing naevus arising from neural crest cells presenting as upper and the lower lid mass

2015 ◽  
Vol 6 (03) ◽  
pp. 417-419 ◽  
Author(s):  
Gian Chand Rajput ◽  
Deepti Mahajan ◽  
Kulbhushan Prakash Chaudhary ◽  
V. Deewana
Keyword(s):  
Visual Acuity ◽  
Malignant Melanoma ◽  
Lower Eyelid ◽  
Melanocytic Lesion ◽  
Visual Axis ◽  
Complete Excision ◽  
Cosmetic Appearance ◽  
The Past ◽  
Upper Lid ◽  
Left Eyelid

ABSTRACTA kissing nevus is a type of congenital compound nevus that affects equal portions of the upper and lower eyelid, and it extends to the lid margins. Congenital divided nevi of the eyelids are a rare melanocytic lesion. Only 30 patients are reported in the literature. We report a 40-year-old female of rural background who presented with a large painless enlarging pigmented mass, involving both upper and lower left eyelid since the past 20 years. Complete excision of the lesion was done, and the mass sent for histopathology, which revealed a compound nevus involving both lids. Surgery removed the obstruction, which had caused decreased visual acuity and had altered the cosmetic appearance of the patient. Lesions on the upper lid cause a mechanical ptosis, covers the visual axis, which causes obstruction in vision and is cosmetically unacceptable. There is a definite risk of malignant change in the nevus giving rise to malignant melanoma, and hence they should be removed as early as possible to give better functional and cosmetic results.

scholarly journals Stick-slip dynamics of cell adhesion triggers spontaneous symmetry breaking and directional migration of mesenchymal cells on one-dimensional lines

2020 ◽  
Vol 6 (1) ◽  
pp. eaau5670 ◽  
Author(s):  
K. Hennig ◽  
I. Wang ◽  
P. Moreau ◽  
L. Valon ◽  
S. DeBeco ◽  
...  
Keyword(s):  
Cell Motility ◽  
Single Cells ◽  
Stick Slip ◽  
One Dimensional ◽  
Migration Assay ◽  
3T3 Fibroblasts ◽  
Cell Substrate ◽  
Nih 3T3 ◽  
Adhesive Contacts

Directional cell motility relies on the ability of single cells to establish a front-rear polarity and can occur in the absence of external cues. The initiation of migration has often been attributed to the spontaneous polarization of cytoskeleton components, while the spatiotemporal evolution of cell-substrate interaction forces has yet to be resolved. Here, we establish a one-dimensional microfabricated migration assay that mimics the complex in vivo fibrillar environment while being compatible with high-resolution force measurements, quantitative microscopy, and optogenetics. Quantification of morphometric and mechanical parameters of NIH-3T3 fibroblasts and RPE1 epithelial cells reveals a generic stick-slip behavior initiated by contractility-dependent stochastic detachment of adhesive contacts at one side of the cell, which is sufficient to trigger cell motility in 1D in the absence of pre-established polarity. A theoretical model validates the crucial role of adhesion dynamics, proposing that front-rear polarity can emerge independently of a complex self-polarizing system.

Comparative analysis of two different types of intracorneal implants in keratoconus: A corneal tomographic study

2020 ◽  
pp. 112067212096344
Author(s):  
Mohammad-Reza Sedaghat ◽  
Hamed Momeni-Moghaddam ◽  
Michael W Belin ◽  
David P Piñero ◽  
Reyhaneh Akbarzadeh ◽  
...  
Keyword(s):  
Visual Acuity ◽  
Spherical Equivalent ◽  
Q Value ◽  
Corneal Surface ◽  
Corrected Distance Visual Acuity ◽  
Different Types ◽  
Corneal Asphericity ◽  
Tomographic Data ◽  
Corneal Shape ◽  
Mean Differences

Objective: To evaluate changes in visual acuity and corneal tomographic outcomes at 6 months after femtosecond-laser assisted implantation of two different types of intracorneal implants in keratoconus. Methods: A total of 39 keratoconus eyes implanted with two segments KeraRing ( n = 22 eyes) or MyoRing ( n = 17 eyes) were enrolled. Tomographic data (Pentacam system, Oculus) were analyzed and correlated with enhancement in uncorrected (UDVA) and corrected distance visual acuity (CDVA). The tomographic indices were front maximum keratometry (Kmax), corneal asphericity (Q-value) on both surfaces, average pachymetric progression indices (PPI), maximum Ambrosio relational thickness (ARTmax), Belin-Ambrósio enhanced ectasia total deviation index (BAD-D), index of surface variance (ISV), vertical asymmetry (IVA), height asymmetry (IHA) and height decentration (IHD). Results: LogMAR UDVA and CDVA improved 2.1 ( p = 0.003) and 0.7 ( p = 0.074) lines with KeraRing, and 8 and 2.5 lines with MyoRing ( p = 0.001). The highest and lowest mean differences in the tomographic indices between both groups were related to ISV and IHD, respectively. Changes in all indices differed significantly between two groups except for changes in front corneal astigmatism, ARTmax, ISV, IVA, IHD and IHA ( p > 0.05). Correlation of changes in CDVA with changes in other parameters was statistically significant only for IHD in the KeraRing group, while changes in in UDVA were significantly correlated with changes in spherical equivalent, back Q-value, ISV, IVA, and IHA only in the MyoRing group. Conclusion: Both implants promote corneal shape regularization and an enhancement in UDVA in keratoconus. A considerable flattening effect and reduction in prolateness in the front corneal surface were observed with MyoRing.

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