scholarly journals Lack of Association betweenTLR4Genetic Polymorphisms and Diabetic Nephropathy in a Chinese Population

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Danfeng Peng ◽  
Jie Wang ◽  
Jiemin Pan ◽  
Rong Zhang ◽  
Shanshan Tang ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Chinese Population ◽  
Diabetic Patients ◽  
Low Grade ◽  
Nucleotide Polymorphisms ◽  
Type 2 Diabetic Patients ◽  
Single Nucleotide ◽  
Patients With Diabetes ◽  
Low Grade Inflammation

Objective. Toll-like receptor 4 (TLR4) plays a central role in innate immunity. Activation of innate immune response and subsequent chronic low-grade inflammation are thought to be involved in the pathogenesis of diabetic nephropathy. In this study, we aimed to investigate whetherTLR4variants are associated with diabetic nephropathy in the Chinese population.Methods. Seven tagging single nucleotide polymorphisms (SNPs) ofTLR4based on HapMap Chinese data were genotyped in 1,455 Chinese type 2 diabetic patients. Of these patients, 622 were diagnosed with diabetic nephropathy and 833 were patients with diabetes for over 5 years but without diabetic nephropathy.Results. None of the SNPs and haplotypes showed significant association to diabetic nephropathy in our study. No association between the SNPs and quantitative traits was observed either.Conclusion. We concluded that common variants withinTLR4genes were not associated with diabetic nephropathy in the Chinese type 2 diabetes patients.

scholarly journals STUDY TO ASSESS ASSOCIATION OF C-REACTIVE PROTEIN WITH NEPHROPATHY IN PEOPLE LIVING WITH TYPE 2 DIABETES MELLITUS

2020 ◽  
Author(s):  
KAUSHIK MONDAL ◽  
DATTATREYA MUKHERJEE
Keyword(s):  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Diabetic Patients ◽  
Low Grade ◽  
C Reactive Protein ◽  
Type 2 Diabetic Patients ◽  
Inflammatory Reactions ◽  
Reactive Protein ◽  
Markers Of Inflammation

Abstract:- Introduction: Diabetes mellitus (DM) is a metabolic disorder with inappropriate hyperglycemia either due to an absolute or relative deficiency of insulin secretion or reduction in the biologic effectiveness of insulin or both. An inflammatory basis for Diabetes & its complications has attracted interest. Among several markers of inflammation, C reactive protein (CRP) is found to be significant in people with diabetes. Diabetic nephropathy may be associated with abnormally high levels of CRP. Materials & Methods: This study was performed for 1 year over 100 type 2 diabetic patients (n=100) aged 30-70 years in Calcutta National Medical College & Hospital. Necessary clinical & Diabetes related laboratory investigations were done as the routine procedure. Results : Among 100 study population, all macro-albuminuria patients were found to have high CRP. 63% of Stage 3 chronic kidney disease (CKD) patients were having high CRP in our study. High CRP values were also found among patients with higher spot urine ACR (329.50+-259.40 mg/gram).(P<0.05) Conclusion : CRP is a marker which increased in inflammatory reactions. Low grade inflammation as indicated by high CRP was an important predictor of diabetic nephropathy.

scholarly journals Effect of MATE 1, MATE 2 and OCT1 Single Nucleotide Polymorphisms on Metformin Action in Recently Diagnosed Egyptian Type-2 Diabetic Patients

2018 ◽  
Vol 11 (1) ◽  
pp. 149-157
Author(s):  
Gomaa Mostafa-Hedeab ◽  
Alaa Abdelhamed Mohamed ◽  
Gamal Thabet ◽  
Dina Sabry ◽  
Randa Fayez Salam ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Detection System ◽  
Diabetic Patients ◽  
Nucleotide Polymorphisms ◽  
Type 2 Diabetic Patients ◽  
Single Nucleotide ◽  
Sequence Detection ◽  
Factors Affecting ◽  
Type 2 Diabetic

Objective: to study the effect of MATE 1, MATE 2 and OCT1 genetic variants on metformin action in recently diagnosed Egyptian Type-2 diabetic patients. Patients & Methods: One hundred type-2 DM patients and forty healthy control were included in the study. All patients were recently diagnosed receiving no treatment before participation in the study. Three single nucleotide polymorphisms (SNPs) were Genotyped using real time PCR, Sequence Detection System: MATE1 (rs2252281), OCT1 coding variants (rs12208357) (SLC22A1) and MATE2 (rs12943590). Results: there is a significant differences between control and patients regarding MATE2 (p<0.05), OCT1 (P<0.005) distribution; in which GG (54%), CC (62%) is the most prevalent among studied patients respectively. MATE1 SNP; Patients with CC alleles and TT allele had better HBA1C (8.577±.2924), (8.7±.25) compared to CT allele patients (9.584±.3023) (P= .04) (P=.019) respectively. OCT1 SNP; CG allele patients showed better RBS (251±9.565) compared to CC allele (294.42±8.476) (p=0.004). Logistic regression test showed that RBS (p=.00001), ALT (p=.0001) and TLC (p=.025) are independent factors affecting blood glucose. Conclusion: MATE1 and OCT1 SNPs may have a potential role in metformin efficacy.

scholarly journals Diabetes Results in Structural Alteration of Chondroitin Sulfate in the Urine

2013 ◽  
Vol 16 (3) ◽  
pp. 486 ◽  
Author(s):  
Tingting Zhao ◽  
Xiaoguang Lu ◽  
Neal M Davies ◽  
Yuewen Gong ◽  
Jingzhen Guo ◽  
...  
Keyword(s):  
Diabetic Nephropathy ◽  
Chondroitin Sulfate ◽  
Chondroitin Sulphate ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Significant Difference ◽  
Patients With Diabetes ◽  
Healthy Control ◽  
The Relationship

Purpose. The assessment of the clinical significance of chondroitin sulfate in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN) for the detection of the relationship between chondroitin sulfate (CS) structure and disease. Methods. Healthy control (n=15), type 2 diabetic patients with normalbuminuria (n=12), and patients with microalbuminuria (n=13) were enrolled in the study. Total sulfated glycosaminoglycans (GAGs) concentration in the first morning urine was evaluated by 1,9-dimethylmethylene blue method and the composition was determined by agarose gel electrophoresis. Urinary chondroitin sulfate was quantified by a combination of treatment with specific lyase digestions and separation of products by SAX-HPLC. Results: GAGs concentration significantly increased in diabetic patients with microalbuminuria compared to diabetic patients with normalbuminuria. Qualitative analysis of urinary GAGs revealed the presence of chondroitin sulfate, heparan sulfate, and low-sulphated chondroitin sulphate-protein complex (LSC-PG). There was a decrease in CS and an increase in LSC-PG in the urine of patients with diabetes compared to healthy controls. Moreover, in diabetic patients, chondroitin sulfate contains more 6-sulfated disaccharide and less 4-sulfated disaccharide. There was a statistically significant difference in ratio of 6-sulfated disaccharide to 4-sulfated disaccharide among the three groups. Conclusions: GAGs were significantly increased in diabetic patients with microalbuminuria. The levels of urinary GAGs, ratio of LSC-PG/CS, as well as ratio of 6-sulfated to 4-sulfated disaccharides could be useful markers for diagnosis of patients with diabetic nephropathy.   This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.

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