scholarly journals Alternating Current Electrohydrodynamic Printing of Microdroplets

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Gao-Feng Zheng ◽  
Hai-Yan Liu ◽  
Rong Xu ◽  
Xiang Wang ◽  
Juan Liu ◽  
...  
Keyword(s):  
Electric Field ◽  
Process Parameters ◽  
Duty Cycle ◽  
Inkjet Printing ◽  
Alternating Current ◽  
Control Technology ◽  
Supply Rate ◽  
Ac Electric Field ◽  
Electrohydrodynamic Printing ◽  
Electric Field Force

This paper discusses the technology of orderly printing of microdroplets by means of electrohydrodynamic print (EHDP) with alternating current (AC). The AC electric field induces charges to reciprocate in the electrohydrodynamic charged jet and generates periodic alternation of electric field force, which facilitates the breakup of charged jets and injection of microdroplets. Microdroplets with a diameter of 100~300 μm can be printed with a frequency of 5~25 Hz via AC EHDP. Effects of process parameters on the microdroplet injection behaviors were investigated. A higher frequency of applied AC voltage led to a higher deposition frequency, but smaller diameters of printed droplets. Deposition frequency and droplet diameters increased with the increase of duty cycle and solution supply rate. AC pulse voltage has provided a novel way to study the control technology in EHDP, which would accelerate the application of inkjet printing in the field of micro/nanosystem production.

Practical application of non-contact alternating current electric field mixing for reagent-saving in situ hybridisation of HER2

2019 ◽  
Vol 72 (9) ◽  
pp. 603-608
Author(s):  
Nobuyasu Kurihara ◽  
Kazuhiro Imai ◽  
Hiroshi Nanjo ◽  
Ryuta Nakamura ◽  
Yuki Wakamatsu ◽  
...  
Keyword(s):  
Electric Field ◽  
In Situ Hybridisation ◽  
Alternating Current ◽  
Her2 Status ◽  
Breast Cancers ◽  
Her2 Positive ◽  
Specific Staining ◽  
New Device ◽  
Ac Electric Field

AimsHuman epidermal growth factor receptor 2 (HER2)-targeted agents are effective against HER2-positive breast cancers. However, their lack of survival benefit in HER2-negative patients as well as their toxic effects and high cost highlight the need for accurate assessment of HER2 status. Our aim was to evaluate the clinical utility of a reagent-saving in situ hybridisation (Saving ISH) that facilitates hybridisation and saves HER2/chromosome enumeration probe by taking advantage of the non-contact mixing effect of an alternating current (AC) electric field.MethodsWith a new device, we apply a high-voltage, low-frequency AC electric field to the tissue sections, which mixes the probe within microdroplets as the voltage is switched on and off. Specimens (n=113) from patients with breast cancers identified immunohistochemically as HER2 0/1(+), (2+) or (3+) were used. The specimens were all tested using conventional dual ISH (DISH), DISH with an automated slide stainer (ASS) and Saving ISH (1:1–1:3 dilution).ResultsThe Saving ISH with 1:2 probe dilution produced stable results with less non-specific staining while using smaller amounts of probe. The accuracy of HER2 status with Saving ISH was equal to standard. We found 96.4% agreement between DISH using ASS and Saving ISH (kappa coefficient=0.912).ConclusionsThese results suggest reagent-saving HER2 ISH could be used as a clinical tool for accurate and stable HER2 assessment, even when reagent concentrations vary.

scholarly journals Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Hiromitsu Shirasawa ◽  
Jin Kumagai ◽  
Emiko Sato ◽  
Katsuya Kabashima ◽  
Yukiyo Kumazawa ◽  
...  
Keyword(s):  
Electric Field ◽  
Three Dimensional ◽  
Alternating Current ◽  
Immunofluorescence Staining ◽  
Ac Electric Field ◽  
Novel Method ◽  
A New Technique ◽  
Antigen Antibody ◽  
First Time ◽  
Mammalian Eggs

Abstract Recently, a new technique was developed for non-catalytically mixing microdroplets. In this method, an alternating-current (AC) electric field is used to promote the antigen–antibody reaction within the microdroplet. Previously, this technique has only been applied to histological examinations of flat structures, such as surgical specimens. In this study, we applied this technique for the first time to immunofluorescence staining of three-dimensional structures, specifically, mammalian eggs. We diluted an antibody against microtubules from 1:1,000 to 1:16,000 and compared the chromatic degree and extent of fading across dilutions. In addition, we varied the frequency of AC electric-field mixing from 5 Hz to 46 Hz and evaluated the effect on microtubule staining. Microtubules were more strongly stained after AC electric-field mixing for only 5 minutes, even when the concentration of primary antibody was 10 times lower than in conventional methods. AC electric-field mixing also alleviated microtubule fading. At all frequencies tested, AC electric-field mixing resulted in stronger microtubule staining than in controls. There was no clear difference in a microtubule staining between frequencies. These results suggest that the novel method could reduce antibody consumption and shorten immunofluorescence staining time.

Indirect oxidation of aluminum under an AC electric field

RSC Advances ◽  
2016 ◽  
Vol 6 (93) ◽  
pp. 90318-90321 ◽  
Author(s):  
Hidetaka Asoh ◽  
Mami Ishino ◽  
Hideki Hashimoto
Keyword(s):  
Electric Field ◽  
Porous Alumina ◽  
Alternating Current ◽  
Indirect Oxidation ◽  
Alumina Films ◽  
Electrical Connection ◽  
Ac Electric Field ◽  
Anodic Porous Alumina ◽  
Current Electric Field

Anodic porous alumina films can be formed by indirect oxidation under an alternating-current electric field without a direct electrical connection.

scholarly journals One-directional flow of ionic solutions along fine electrodes under an alternating current electric field

2019 ◽  
Vol 6 (2) ◽  
pp. 180657
Author(s):  
Jung Hwal Shin ◽  
Kanghyun Kim ◽  
Hyeonsu Woo ◽  
In Seok Kang ◽  
Hyun-Wook Kang ◽  
...  
Keyword(s):  
Electric Field ◽  
Electric Fields ◽  
Alternating Current ◽  
Ion Concentration ◽  
Flow Rates ◽  
Directional Flow ◽  
Research Fields ◽  
Offset Voltage ◽  
Ac Electric Field ◽  
Dc Electric Field

Electric fields are widely used for controlling liquids in various research fields. To control a liquid, an alternating current (AC) electric field can offer unique advantages over a direct current (DC) electric field, such as fast and programmable flows and reduced side effects, namely the generation of gas bubbles. Here, we demonstrate one-directional flow along carbon nanotube nanowires under an AC electric field, with no additional equipment or frequency matching. This phenomenon has the following characteristics: First, the flow rates of the transported liquid were changed by altering the frequency showing Gaussian behaviour. Second, a particular frequency generated maximum liquid flow. Third, flow rates with an AC electric field (approximately nanolitre per minute) were much faster than those of a DC electric field (approximately picolitre per minute). Fourth, the flow rates could be controlled by changing the applied voltage, frequency, ion concentration of the solution and offset voltage. Our finding of microfluidic control using an AC electric field could provide a new method for controlling liquids in various research fields.

AC electric field induced dipole-based on-chip 3D cell rotation

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2717-2727 ◽  
Author(s):  
Prateek Benhal ◽  
J. Geoffrey Chase ◽  
Paul Gaynor ◽  
Björn Oback ◽  
Wenhui Wang
Keyword(s):  
Electric Field ◽  
Cost Effective ◽  
Alternating Current ◽  
First Report ◽  
Ac Electric Field ◽  
Cell Rotation ◽  
Induced Dipole ◽  
On Chip ◽  
Current Electric Field

First report on 3D rotation of cells using alternating current electric field on a single, open-top, and cost effective biochip.

scholarly journals Effect of Oxidative Stress on Physicochemical Quality of Taiwanese Seagrape (Caulerpa lentillifera) with the Application of Alternating Current Electric Field (ACEF) during Post-Harvest Storage

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1011
Author(s):  
Andi Syahrullah Sulaimana ◽  
Chao-Kai Chang ◽  
Chih-Yao Hou ◽  
Bara Yudhistira ◽  
Fuangfah Punthi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Electric Field ◽  
Alternating Current ◽  
Chlorophyll Degradation ◽  
Physicochemical Quality ◽  
Post Harvest ◽  
Caulerpa Lentillifera ◽  
Different Seasons ◽  
Current Electric Field

This study aims to determine the physicochemical quality of seagrape (Caulerpa lentillifera) as a freshness label for products cultivated in different seasons. The applied post-harvest storage experiments compared between, within and without seawater that led to oxidative stress conditions. Water content, malondialdehyde (MDA) compound, total phenolic content (TPC), and chlorophyll content were observed at 0, 3, 6, and 9 days of storage. The storage without seawater showed sharper quality reductions by reaching 20–40% of water loss, 70–90% of MDA production, 15–25% of TPC reduction, and 40–60% of total chlorophyll degradation. The storage within seawater showed lower quality reductions due to the specific growth rates still reaching 5–10%. This study found that the greater the physicochemical quality, the slower the decomposition rates of the stored seagrape during storage. Therefore, the seagrapes’ obvious discoloration occurred earlier in winter, followed by summer and spring. Kinetics of chlorophyll degradation on seagrape in different seasons meet different order-reactions during storage. Furthermore, alternating current electric field (ACEF) treatment with 125 kV/m of intensity for 60 min can lower the spring seagrapes’ physicochemical quality by reaching 10–30% of inhibition, resulting in the shelf-life extension for up to 12 days of post-harvest storage.

Simulation of TiO 2 particle trajectory in AC electric field

2015 ◽  
Vol 108 ◽  
pp. 183-191 ◽  
Author(s):  
Reza Riahifar ◽  
Babak Raissi ◽  
Cyrus Zamani ◽  
Ehsan Marzbanrad
Keyword(s):  
Electric Field ◽  
Particle Trajectory ◽  
Ac Electric Field
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