scholarly journals Novel method for immunofluorescence staining of mammalian eggs using non-contact alternating-current electric-field mixing of microdroplets

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Hiromitsu Shirasawa ◽  
Jin Kumagai ◽  
Emiko Sato ◽  
Katsuya Kabashima ◽  
Yukiyo Kumazawa ◽  
...  
Keyword(s):  
Electric Field ◽  
Three Dimensional ◽  
Alternating Current ◽  
Immunofluorescence Staining ◽  
Ac Electric Field ◽  
Novel Method ◽  
A New Technique ◽  
Antigen Antibody ◽  
First Time ◽  
Mammalian Eggs

Abstract Recently, a new technique was developed for non-catalytically mixing microdroplets. In this method, an alternating-current (AC) electric field is used to promote the antigen–antibody reaction within the microdroplet. Previously, this technique has only been applied to histological examinations of flat structures, such as surgical specimens. In this study, we applied this technique for the first time to immunofluorescence staining of three-dimensional structures, specifically, mammalian eggs. We diluted an antibody against microtubules from 1:1,000 to 1:16,000 and compared the chromatic degree and extent of fading across dilutions. In addition, we varied the frequency of AC electric-field mixing from 5 Hz to 46 Hz and evaluated the effect on microtubule staining. Microtubules were more strongly stained after AC electric-field mixing for only 5 minutes, even when the concentration of primary antibody was 10 times lower than in conventional methods. AC electric-field mixing also alleviated microtubule fading. At all frequencies tested, AC electric-field mixing resulted in stronger microtubule staining than in controls. There was no clear difference in a microtubule staining between frequencies. These results suggest that the novel method could reduce antibody consumption and shorten immunofluorescence staining time.

Effect of Three-Dimensional Electric Field and Heat Conduction to Electrodes on the Temperature Rise During Irreversible Electroporation

2011 ◽  
Author(s):  
Seiji Nomura ◽  
Kosaku Kurata ◽  
Hiroshi Takamatsu
Keyword(s):  
Heat Conduction ◽  
Electric Field ◽  
Temperature Rise ◽  
Heat Conduction Equation ◽  
Thermal Damage ◽  
Irreversible Electroporation ◽  
Three Dimensional ◽  
End Effects ◽  
Abnormal Cells ◽  
Novel Method

The irreversible electroporation (IRE) is a novel method to ablate abnormal cells by applying a high voltage between two electrodes that are stuck into abnormal tissues. One of the advantages of the IRE is that the extracellular matrix (ECM) may be kept intact, which is favorable for healing. For a successful IRE, it is therefore important to avoid thermal damage of ECM resulted from the Joule heating within the tissue. A three-dimensional (3-D) analysis was conducted in this study to predict temperature rise during the IRE. The equation of electric field and the heat conduction equation were solved numerically by a finite element method. It was clarified that the highest temperature rise occurred at the base of electrodes adjacent to the insulated surface. The result was significantly different from a two-dimensional (2-D) analysis due to end effects, suggesting that the 3-D analysis is required to determine the optimal condition.

scholarly journals Alternating Current Electrohydrodynamic Printing of Microdroplets

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Gao-Feng Zheng ◽  
Hai-Yan Liu ◽  
Rong Xu ◽  
Xiang Wang ◽  
Juan Liu ◽  
...  
Keyword(s):  
Electric Field ◽  
Process Parameters ◽  
Duty Cycle ◽  
Inkjet Printing ◽  
Alternating Current ◽  
Control Technology ◽  
Supply Rate ◽  
Ac Electric Field ◽  
Electrohydrodynamic Printing ◽  
Electric Field Force

This paper discusses the technology of orderly printing of microdroplets by means of electrohydrodynamic print (EHDP) with alternating current (AC). The AC electric field induces charges to reciprocate in the electrohydrodynamic charged jet and generates periodic alternation of electric field force, which facilitates the breakup of charged jets and injection of microdroplets. Microdroplets with a diameter of 100~300 μm can be printed with a frequency of 5~25 Hz via AC EHDP. Effects of process parameters on the microdroplet injection behaviors were investigated. A higher frequency of applied AC voltage led to a higher deposition frequency, but smaller diameters of printed droplets. Deposition frequency and droplet diameters increased with the increase of duty cycle and solution supply rate. AC pulse voltage has provided a novel way to study the control technology in EHDP, which would accelerate the application of inkjet printing in the field of micro/nanosystem production.

AC electric field controlled non-Newtonian filament thinning and droplet formation on the microscale

Lab on a Chip ◽  
2017 ◽  
Vol 17 (17) ◽  
pp. 2969-2981 ◽  
Author(s):  
Y. Huang ◽  
Y. L. Wang ◽  
T. N. Wong
Keyword(s):  
Flow Field ◽  
Electric Field ◽  
Newtonian Fluid ◽  
High Speed ◽  
Droplet Formation ◽  
Particle Imaging Velocimetry ◽  
Ac Electric Field ◽  
Formation Dynamics ◽  
Particle Imaging ◽  
First Time

We investigate the AC electric field controlled filament thinning and droplet formation dynamics of one non-Newtonian fluid. Furthermore, for the first time, we quantitatively measure the flow field of the non-Newtonian droplet formation under the influence of AC electric field, via a high-speed micro particle imaging velocimetry (μPIV) system. We discover the viscoelasticity contributes to the discrepancies majorly.

Combination of Au Dielectrophoresis Chip and Optical Tweezers for Cell Culture

2015 ◽  
Vol 656-657 ◽  
pp. 549-553
Author(s):  
Kyohei Nishimoto ◽  
Kozo Taguchi
Keyword(s):  
Electric Field ◽  
Optical Tweezers ◽  
Cell Separation ◽  
Low Cost ◽  
Three Dimensional ◽  
Uniform Electric Field ◽  
Objective Lens ◽  
Separation System ◽  
Ac Electric Field ◽  
Viable Cells

Dielectrophoresis (DEP) force will arise when an inhomogeneous AC electric field with sinusoidal wave is applied to microelectrodes. By using DEP, we could distinguish between viable and non-viable cells by their movement through a non-uniform electric field. In this paper, we propose a yeast cell separation system, which utilizes an Au DEP chip and an optical tweezers. The Au DEP chip is planar quadrupole microelectrodes, which were fabricated by Au thin-film and a box cutter. This fabrication method is low cost and simpler than previous existing methods. The tip of the optical tweezers was fabricated by dynamic chemical etching in a mixture of hydrogen fluoride and toluene. The optical tweezers has the feature of high manipulation performance. That does not require objective lens for focusing light because the tip of optical tweezers has conical shape. By using both the Au DEP chip and optical tweezers, we could obtain three-dimensional manipulation of specific cells after viability separation.

Morphology of the cephalothorax integument of Bryocamptus pygmaeus (Copepoda: Harpacticoida: Canthocamptidae), based on a new research method

2021 ◽  
Vol 30 (2) ◽  
pp. 320-330
Author(s):  
A.A. Novikov ◽  
E.B. Fefilova
Keyword(s):  
Three Dimensional ◽  
Komi Republic ◽  
Southeastern Part ◽  
The North ◽  
Udmurt Republic ◽  
The Republic ◽  
A New Technique ◽  
Three Dimensional Models ◽  
New Research ◽  
First Time

The number and location of sensilla and pores of the cephalothorax integument of the species Bryocamptus pygmaeus (G.O. Sars, 1863) (Copepoda, Canthocamptidae) were studied for the first time on the material from several European regions: the southeastern part of the Bolshezemelskaya tundra (Ne­nets Autonomous District), the north of the Komi Republic, the Republic of Karelia, and the central part of European Russia (Udmurt Republic). In the samples examined, two groups of populations differing in the characters of cephalothorax integument were recognised. These differences were found to correlate with the variability of the endopod of fourth pair of female legs, which bears four setae in specimens of the eastern form, while a specimen examined from Karelia has five setae. Pore maps are composed for both groups of morphotypes. A statistical analysis was carried out based on a new technique using three-dimensional models of the cephalothorax. As a result of this analysis, a high similarity between individuals of the eastern form and differences of the latter from the Karelian specimen were revealed.

A novel method for determining the X-ray beam angulation of the supraspinatus outlet view

2021 ◽  
pp. 028418512110438
Author(s):  
Xiaoli Zheng ◽  
Jingying Hu ◽  
Jian Xu ◽  
Zhen Wang ◽  
Zhenyu Shu ◽  
...  
Keyword(s):  
Success Rate ◽  
Pearson Correlation ◽  
Three Dimensional ◽  
The Novel ◽  
Ct Reconstruction ◽  
Beam Angle ◽  
X Ray ◽  
Horizontal Angle ◽  
Novel Method ◽  
First Time

Background Rapid and accurate quantification of the supraspinatus outlet view (SOV) is a clinical challenge. Purpose To quantify the X-ray beam angle of the SOV using the horizontal angle of the subscapular spine line (SSSL) and to further verify the feasibility of this method. Material and Methods A total of 119 patients who underwent shoulder computed tomography (CT) examination were enrolled in the retrospective study. Three-dimensional (3D) CT reconstruction was performed and manually adjusted to provide the position similar to SOV. The rotation angle of the 3D image along the long axis of the human body (marked as β) was obtained. The horizontal angle of SSSL (marked as α) was measured on the anteroposterior localizer image of shoulder CT. Pearson correlation and linear regression correlation analysis were performed. In addition, the first-time success rate between the experience-based group and the measurement-based group were compared to verify the novel method. Results We found a linear correlation between α and β (r = 0.962; P = 0.000). There was no significant correlation between the experience-based group and the measurement-based group in terms of age ( P = 0.500), sex ( P = 0.397), and side ( P = 0.710), but there was a significant statistical difference in the first success rate between the two validation groups (χ2 = 5.808a, P = 0.016). Conclusion This novel quantitative measurement method for determining the X-ray beam angle of SOV using the horizontal angle of SSSL is feasible.

scholarly journals Novel method for rapid in-situ hybridization of HER2 using non-contact alternating-current electric-field mixing

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Yoshitaro Saito ◽  
Kazuhiro Imai ◽  
Ryuta Nakamura ◽  
Hiroshi Nanjo ◽  
Kaori Terata ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Electric Field ◽  
Alternating Current ◽  
Novel Method ◽  
Current Electric Field
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