scholarly journals Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone inPichia pastoris

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hussin A. Rothan ◽  
Teh Ser Huy ◽  
Zulqarnain Mohamed
Keyword(s):  
Growth Hormone ◽  
Large Scale ◽  
Culture Supernatant ◽  
Codon Optimization ◽  
Fish Growth ◽  
Absolute Methanol ◽  
Growth Hormone Gene ◽  
Fish Feed ◽  
Scale Production ◽  
Feed Supplements

This study was established to test the hypothesis of whether the codon optimization of fish growth hormone gene (FGH) based onP. pastorispreferred codon will improve the quantity of secreted rFGH in culture supernatant that can directly be used as fish feed supplements. The optimizedFGHcoding sequence (oFGH) and native sequence (nFGH) of giant grouper fish (Epinephelus lanceolatus) were cloned intoP. pastorisexpression vector (pPICZαA) downstream ofalcohol oxidasegene (AOX1) for efficient induction of extracellular rFGH by adding 1% of absolute methanol. The results showed that recombinantP. pastoriswas able to produce2.80±0.27 mg of oFGH compared to1.75±0.25of nFGH in one litre of culture supernatant. The total body weight of tiger grouper fingerlings fed with oFGH increased significantly at third (P<0.05) and fourth weeks (P<0.01) of four-week experiment period compared to those fed with nFGH. Both oFGH and nFGH significantly enhanced the final biomass and fish survival percentage. In conclusion, codon optimization ofFGHfragment was useful to increase rFGH quantity in the culture supernatant ofP. pastoristhat can be directly used as fish feed supplements. Further studies are still required for large scale production of rFGH and practical application in aquaculture production.

Expression of human growth hormone gene in Pleurotus eryngii

2010 ◽  
Vol 5 (6) ◽  
pp. 791-799 ◽  
Author(s):  
Seung Kim ◽  
Kumar Sapkota ◽  
Bong-Suk Choi ◽  
Sung-Jun Kim
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Large Scale ◽  
Recombinant Human Growth Hormone ◽  
Human Growth ◽  
Pleurotus Eryngii ◽  
Growth Hormone Gene ◽  
Scale Production ◽  
Western Blot Assay ◽  
Human Growth Hormone Gene

AbstractRecombinant human growth hormone (rhGH) has been widely used in many medical applications. Large scale production of rhGH is important for a wider application of this molecule in the field of proteomics. This investigation reports a modified Agrobacterium-mediated method for transfer and expression of human growth hormone gene in the popular edible mushroom Pleurotus eryngii. A binary vector pCAMBIA1304 containing the hGH2 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. Integration of hGH2 into mushroom genome was detected by PCR and expression was confirmed by Western blot assay. The successful expression of the human growth hormone gene in mushroom suggests that the proposed modified transformation system is probably useful for the production of transgenic mushrooms.

Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene

2001 ◽  
Vol 59 (1) ◽  
pp. 62-78 ◽  
Author(s):  
M. A. Rahman ◽  
A. Ronyai ◽  
B. Z. Engidaw ◽  
K. Jauncey ◽  
G-L. Hwang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Nile Tilapia ◽  
Fish Growth ◽  
Growth Hormone Gene

Genetic analysis of “all-fish” growth hormone gene trans ferred carp (Cyprinus carpio L.) and its F1 generation

2001 ◽  
Vol 46 (14) ◽  
pp. a1-a4 ◽  
Author(s):  
Yaping Wang ◽  
Wei Hu ◽  
Gang Wu ◽  
Yonghua Sun ◽  
Shangping Chen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Genetic Analysis ◽  
Cyprinus Carpio ◽  
Fish Growth ◽  
Growth Hormone Gene ◽  
Carp Cyprinus Carpio ◽  
F1 Generation

Rapid growth cost in “all-fish” growth hormone gene transgenic carp: Reduced critical swimming speed

2007 ◽  
Vol 52 (11) ◽  
pp. 1501-1506 ◽  
Author(s):  
DeLiang Li ◽  
CuiZhang Fu ◽  
Wei Hu ◽  
Shan Zhong ◽  
YaPing Wang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Swimming Speed ◽  
Rapid Growth ◽  
Fish Growth ◽  
Growth Hormone Gene ◽  
Critical Swimming Speed

Changthangi Goats: A rich source of pashmina production in Ladakh

2004 ◽  
Vol 35 ◽  
pp. 75-85 ◽  
Author(s):  
T.K. Bhattacharya ◽  
S.S. Misra ◽  
F.D. Sheikh ◽  
P. Kumar ◽  
A. Sharma
Keyword(s):  
Growth Hormone ◽  
Large Scale ◽  
Fibre Diameter ◽  
The Body ◽  
Whole Body ◽  
Growth Hormone Gene ◽  
Body Colour ◽  
Male And Female ◽  
Pcr Rflp ◽  
Preliminary Study

SummaryPashmina, internationally known as “cashmere”, a fine luxury fibre, is being produced from Changthangi goats bred in the Ladakh region of India. The Leh district of Greater Ladakh produces around 30 000 kg of pashmina fibre which is harvested from about 0.15 million Changthangi goats reared by the Changpa nomads in Changthang region of Greater Ladakh. Changthangi goats are sometimes also called Changra goats. Pashmina producing goats are of great importance for revitalising the economy of the poverty stricken region of Changthang and the Leh district of Ladakh. The information on Changthangi goats was collected from both small and large-scale farmers in Ladakh, and 337 animals were included in the study.The body colour of Changthangi goats varies from white to light brown and nearly whole body is covered with pashmina and long hairs. The average birth weights of male and female kids were estimated as 2.11 ± 0.3 and 2.06 ± 0.2 kg, respectively while weight at 300 days was found to be 20.0 ± 2.1 and 18.7 ± 1.9 kg in male and females respectively.The pashmina yields of bucks, does, male hoggets and female hoggets were estimated as 402 ± 19 g, 248 ± 14 g, 255 ± 12 and 280 ± 16 g, respectively. The length of pashmina fibre was found to be 4.25 ± 1.2 cm in males and 4.02 ± 1.5 cm in females while fibre diameter in male and female goats was estimated as 12.9 ± 2.6 μ and 13.0 ± 3.0 μ, respectively.The twining rate was found to be very low, nearly 0.3%. A preliminary study at DNA level with PCR-RFLP indicated monomorphism at the growth hormone gene. The occurrence of disease was quite low although some genetic deformities in this breed were not uncommon.

scholarly journals ISOLATION OF Β-ACTIN PROMOTER DERIVED FROM WALKING CATFISH (CLARIAS BATRACHUS)

2015 ◽  
Vol 2 (1) ◽  
pp. 201
Author(s):  
Ibnu Dwi Buwono ◽  
Nono Carsono ◽  
Yuniar Mulyani ◽  
Mochamad Untung Kurnia Agung
Keyword(s):  
Growth Hormone ◽  
Expression Vector ◽  
Fish Growth ◽  
Clarias Batrachus ◽  
Growth Hormone Gene ◽  
Economic Traits ◽  
Food Material ◽  
Vector Construction ◽  
Reverse Primer ◽  
Actin Promoter

Fish growth improvement as economic traits can be solved through fish transgenic production. Growth hormone gene is inserted into transgenic vector construction to over-express fish growth. The promoter as a part of the expression vector has an important role in its regulation. The use of promoter which is derived from mammalian or virus (such as CMV/ cytomegalovirus) in the expression vector, in specific goal as food material, has customer resistant rather than a promoter which is derived from in- sibling species. Beside of it, transgene expression level when using in-sibling promoter showed higher than using mammalian or viral promoter. The β-actin promoter is screened from walking catfish pituitary genome DNA using primers: pBA-cy-F (5’- GTGWGTGACGCYGGACCAAATC-3’) as forward primer and pBA-cy-R (5’- CCATRTCRTCCCAGTTGGTSACAAT-3’) as reverse primer, produced an amplicon of 1,7 kb in length. Sequence analysis using TF BindTM indicated transcription factor elements: TATA box, CCAAT box, enhancer (CAAT), and CarGG (CAAATGG) motif. This result showed that promoter which is obtained from this research is useful in construction of all catfish growth hormone vector expression catfish transgenic production. Keywords: β-actin promoter, growth hormone, expression vector, walking catfish (Clarias batrachus) 

Large scale production of recombinant mouse and rat growth hormone by fed-batch GS-NSO cell cultures

1996 ◽  
Vol 22 (1-3) ◽  
pp. 239-250 ◽  
Author(s):  
Weichang Zhou ◽  
Theodora Bibila ◽  
Konstantin Glazomitsky ◽  
Javier Montalyo ◽  
Christine Chan ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Cell Cultures ◽  
Large Scale ◽  
Scale Production ◽  
Fed Batch ◽  
Recombinant Mouse ◽  
Large Scale Production ◽  
Rat Growth

Growth and energy budget of F2'all-fish' growth hormone gene transgenic common carp

2007 ◽  
Vol 70 (2) ◽  
pp. 347-361 ◽  
Author(s):  
C. Fu ◽  
D. Li ◽  
W. Hu ◽  
Y. Wang ◽  
Z. Zhu
Keyword(s):  
Growth Hormone ◽  
Common Carp ◽  
Energy Budget ◽  
Fish Growth ◽  
Growth Hormone Gene
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