scholarly journals Selective Activation of Fish Growth Hormone Gene Promoters by Rainbow Trout Pit-1

1999 ◽  
Vol 65 (5) ◽  
pp. 802-803
Author(s):  
Shoichi Yamada ◽  
Shinya Yamashita
Keyword(s):  
Growth Hormone ◽  
Rainbow Trout ◽  
Fish Growth ◽  
Growth Hormone Gene ◽  
Gene Promoters ◽  
Selective Activation

Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene

2001 ◽  
Vol 59 (1) ◽  
pp. 62-78 ◽  
Author(s):  
M. A. Rahman ◽  
A. Ronyai ◽  
B. Z. Engidaw ◽  
K. Jauncey ◽  
G-L. Hwang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Nile Tilapia ◽  
Fish Growth ◽  
Growth Hormone Gene

Genetic analysis of “all-fish” growth hormone gene trans ferred carp (Cyprinus carpio L.) and its F1 generation

2001 ◽  
Vol 46 (14) ◽  
pp. a1-a4 ◽  
Author(s):  
Yaping Wang ◽  
Wei Hu ◽  
Gang Wu ◽  
Yonghua Sun ◽  
Shangping Chen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Genetic Analysis ◽  
Cyprinus Carpio ◽  
Fish Growth ◽  
Growth Hormone Gene ◽  
Carp Cyprinus Carpio ◽  
F1 Generation

Body composition of transgenic common carp, Cyprinus carpio, containing rainbow trout growth hormone gene

Aquaculture ◽  
1995 ◽  
Vol 138 (1-4) ◽  
pp. 99-109 ◽  
Author(s):  
N. Chatakondi ◽  
R.T. Lovell ◽  
P.L. Duncan ◽  
M. Hayat ◽  
T.T. Chen ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Body Composition ◽  
Rainbow Trout ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Growth Hormone Gene ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

Rapid growth cost in “all-fish” growth hormone gene transgenic carp: Reduced critical swimming speed

2007 ◽  
Vol 52 (11) ◽  
pp. 1501-1506 ◽  
Author(s):  
DeLiang Li ◽  
CuiZhang Fu ◽  
Wei Hu ◽  
Shan Zhong ◽  
YaPing Wang ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Swimming Speed ◽  
Rapid Growth ◽  
Fish Growth ◽  
Growth Hormone Gene ◽  
Critical Swimming Speed

scholarly journals Effect of Codon Optimisation on the Production of Recombinant Fish Growth Hormone inPichia pastoris

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Hussin A. Rothan ◽  
Teh Ser Huy ◽  
Zulqarnain Mohamed
Keyword(s):  
Growth Hormone ◽  
Large Scale ◽  
Culture Supernatant ◽  
Codon Optimization ◽  
Fish Growth ◽  
Absolute Methanol ◽  
Growth Hormone Gene ◽  
Fish Feed ◽  
Scale Production ◽  
Feed Supplements

This study was established to test the hypothesis of whether the codon optimization of fish growth hormone gene (FGH) based onP. pastorispreferred codon will improve the quantity of secreted rFGH in culture supernatant that can directly be used as fish feed supplements. The optimizedFGHcoding sequence (oFGH) and native sequence (nFGH) of giant grouper fish (Epinephelus lanceolatus) were cloned intoP. pastorisexpression vector (pPICZαA) downstream ofalcohol oxidasegene (AOX1) for efficient induction of extracellular rFGH by adding 1% of absolute methanol. The results showed that recombinantP. pastoriswas able to produce2.80±0.27 mg of oFGH compared to1.75±0.25of nFGH in one litre of culture supernatant. The total body weight of tiger grouper fingerlings fed with oFGH increased significantly at third (P<0.05) and fourth weeks (P<0.01) of four-week experiment period compared to those fed with nFGH. Both oFGH and nFGH significantly enhanced the final biomass and fish survival percentage. In conclusion, codon optimization ofFGHfragment was useful to increase rFGH quantity in the culture supernatant ofP. pastoristhat can be directly used as fish feed supplements. Further studies are still required for large scale production of rFGH and practical application in aquaculture production.

Cortisol stimulates growth hormone gene expression in rainbow trout leucocytes in vitro

2005 ◽  
Vol 142 (1-2) ◽  
pp. 248-255 ◽  
Author(s):  
Takashi Yada ◽  
Kohji Muto ◽  
Teruo Azuma ◽  
Susumu Hyodo ◽  
Carl B. Schreck
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Rainbow Trout ◽  
Growth Hormone Gene

scholarly journals ISOLATION OF Β-ACTIN PROMOTER DERIVED FROM WALKING CATFISH (CLARIAS BATRACHUS)

2015 ◽  
Vol 2 (1) ◽  
pp. 201
Author(s):  
Ibnu Dwi Buwono ◽  
Nono Carsono ◽  
Yuniar Mulyani ◽  
Mochamad Untung Kurnia Agung
Keyword(s):  
Growth Hormone ◽  
Expression Vector ◽  
Fish Growth ◽  
Clarias Batrachus ◽  
Growth Hormone Gene ◽  
Economic Traits ◽  
Food Material ◽  
Vector Construction ◽  
Reverse Primer ◽  
Actin Promoter

Fish growth improvement as economic traits can be solved through fish transgenic production. Growth hormone gene is inserted into transgenic vector construction to over-express fish growth. The promoter as a part of the expression vector has an important role in its regulation. The use of promoter which is derived from mammalian or virus (such as CMV/ cytomegalovirus) in the expression vector, in specific goal as food material, has customer resistant rather than a promoter which is derived from in- sibling species. Beside of it, transgene expression level when using in-sibling promoter showed higher than using mammalian or viral promoter. The β-actin promoter is screened from walking catfish pituitary genome DNA using primers: pBA-cy-F (5’- GTGWGTGACGCYGGACCAAATC-3’) as forward primer and pBA-cy-R (5’- CCATRTCRTCCCAGTTGGTSACAAT-3’) as reverse primer, produced an amplicon of 1,7 kb in length. Sequence analysis using TF BindTM indicated transcription factor elements: TATA box, CCAAT box, enhancer (CAAT), and CarGG (CAAATGG) motif. This result showed that promoter which is obtained from this research is useful in construction of all catfish growth hormone vector expression catfish transgenic production. Keywords: β-actin promoter, growth hormone, expression vector, walking catfish (Clarias batrachus) 

The Turkey Transcription Factor Pit-1/GHF-1 Can Activate the Turkey Prolactin and Growth Hormone Gene Promoters in Vitro but Is Not Detectable in Lactotrophs in Vivo

2001 ◽  
Vol 123 (3) ◽  
pp. 244-253 ◽  
Author(s):  
Kristy L. Weatherly ◽  
Ramachandran Ramesh ◽  
Heather Strange ◽  
Kerry L. Waite ◽  
Brian Storrie ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transcription Factor ◽  
Growth Hormone Gene ◽  
Gene Promoters

Growth hormone increases predation exposure of rainbow trout

1996 ◽  
Vol 263 (1370) ◽  
pp. 647-651 ◽  
Keyword(s):  
Growth Hormone ◽  
Rainbow Trout ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Transgenic Fish ◽  
Wild Fish ◽  
Fish Growth ◽  
Genetic Manipulations ◽  
Feeding Motivation ◽  
Energetic State

The energetic state of an animal strongly influences decisions that balances feeding against predation risk. Growth hormone increases the metabolic demands, which should elevate the feeding motivation of an animal. This, in turn, may increase the willingness to risk exposure to predators during feeding. To test this hypothesis, we studied the effect of growth hormone on the behavioural response of rainbow trout ( Oncorhynchus mykiss ) to simulated attacks from a model heron. After attacks, growth hormone treated trout foraged closer to the water surface, resumed feeding earlier, and ate more food than did control trout. Such behaviour should increase the susceptibility to aerial predation. Thus, predation may select against high endogenous growth hormone secretion in wild fish. Furthermore, genetic manipulations to increase growth hormone levels, intended to improve growth performance in aquaculture, may result in individuals with substantially altered behavioural patterns. In light of the increasing potential for interactions between farmed and wild fish, growth hormone transgenic fish may pose a threat to wild fish populations.

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