scholarly journals Cytocompatibility and Antibacterial Properties of Capping Materials

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Claudio Poggio ◽  
Carla Renata Arciola ◽  
Riccardo Beltrami ◽  
Annachiara Monaco ◽  
Alberto Dagna ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Disc Diffusion ◽  
Streptococcus Salivarius ◽  
Diffusion Test ◽  
Streptococcus Sanguis ◽  
Agar Disc ◽  
Bacterial Growth Inhibition

The aim of this study was to evaluate and compare the antimicrobial activity and cytocompatibility of six different pulp-capping materials: Dycal (Dentsply), Calcicur (Voco), Calcimol LC (Voco), TheraCal LC (Bisco), MTA Angelus (Angelus), and Biodentine (Septodont). To evaluate antimicrobial activity, materials were challengedin vitrowithStreptococcus mutans,Streptococcus salivarius, andStreptococcus sanguisin the agar disc diffusion test. Cytocompatibility of the assayed materials towards rat MDPC-23 cells was evaluated at different times by both MTT and apoptosis assays. Results significantly differed among the different materials tested. Both bacterial growth inhibition halos and cytocompatibility performances were significantly different among materials with different composition. MTA-based products showed lower cytotoxicity and valuable antibacterial activity, different from calcium hydroxide-based materials, which exhibited not only higher antibacterial activity but also higher cytotoxicity.

Comparative studies of the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola on Klebsiella pneumonia and Candida albicans in vitro

2021 ◽  
Vol 16 (1) ◽  
pp. 52-58
Author(s):  
Elizabeth Winful ◽  
Olanikpekun Idowu ◽  
Opeoluwa O. Fasanya ◽  
Nkechi E. Egbe
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Ethanol Extract ◽  
Antimicrobial Effect ◽  
Klebsiella Pneumonia ◽  
Disc Diffusion ◽  
Diffusion Test ◽  
Garcinia Kola ◽  
Zones Of Inhibition

Antimicrobial resistance has become a global health problem. Although a wide range of chemotherapeutic antimicrobials are available for treatment of microbial related infections and diseases, development of resistance to these chemotherapeutic agents is rapidly on the increase. Extracts from some plants have shown some promise in antimicrobial activity. This has led to the screening of several medicinal plants for their potential antimicrobial activity. Therefore, this study was aimed at evaluating the antimicrobial effect of ethanolic and aqueous extracts of Garcinia kola against Klebsiella pneumonia and Candida albicans in vitro. The Garcinia kola seeds extracts were obtained using 70% ethanol and distilled water respectively. Phytochemical screening of Garcinia kola revealed the presence of various potent phytochemicals such as tannins, saponins, flavonoids, alkaloids and glycosides. Both extracts of the seeds were investigated for antimicrobial activity using disc diffusion and agar well diffusion sensitivity tests. The ethanol extract produced zones of inhibition of about 7.3 mm for Candida albicans only at a concentration of 800mg/ml for the disc diffusion test. For the agar well diffusion test, the aqueous extract produced zones of inhibition of about 9.5 mm, while the ethanol extract produced zone of inhibition of 19 mm against Candida albicans at a concentration of 800 mg/ml. However, at the same concentration, the ethanol extract produced zones of inhibition of about 8.5 mm against Klebsiella pneumoniae. The findings of this study revealed that Garcinia kola was not effective in treating Klebsiella pneumonia infections but has potential in treating Candida albicans infections. Keywords: Garcinia kola, Klebsiella pneumonia, Candida albicans

scholarly journals Antibacterial Properties of Local Malaysian Trigona Sp. Honey Towards Different of Pathogenic Bacteria in Vitro

2021 ◽  
Vol 13 (1) ◽  
pp. 1-9
Author(s):  
Fawzia Jassim Shalsh ◽  
Ahmed Mohammed Khalaf ◽  
Mohammed Hafiz ◽  
Imad Ibrahim Ali
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Minimum Bactericidal Concentration ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Therapeutic Effects ◽  
Disc Diffusion ◽  
Diffusion Test

Honey is one of the oldest natural medicines known with a very high therapeutic value. Nowadays, in the medical field, several important therapeutic effects of honey have been elucidated. This study was conducted to reveal the antimicrobial activity of the commercially available local Malaysian Trigona sp. honey towards different pathogenic bacteria specifically Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Salmonella typhimurium. The Minimum inhibitory concentration and Minimum bactericidal concentration were determined by the disc-diffusion test and agar well diffusion test. Different concentration of the honey was tested in the disc-diffusion and agar well diffusion test. The results of these tests were in terms of Inhibition zone diameter. The results obtained from the current study are the dilution of different concentration of honey from Trigona sp. are very significant because the only net concentration of both of honey Trigon a sp. possessed antimicrobial properties in term of Minimum inhibitory concentration) and Minimum bactericidal concentration. The result also can say that Trigona sp. honey possessed antibacterial properties and can be used as alternative medicine in the veterinary field in the future.

scholarly journals Preliminary in vitro screening of the antibacterial activity of leaf extracts from various Ficus species (Moraceae) against Yersinia ruckeri

2019 ◽  
Vol 27 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Halyna Tkachenko ◽  
Lyudmyla Buyun ◽  
El¿bieta Terech-Majewska ◽  
Vitaliy Honcharenko ◽  
Andriy Prokopiv ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Yersinia Ruckeri ◽  
Leaf Extracts ◽  
Fish Pathogens ◽  
Promising Alternative ◽  
Ethanolic Extracts ◽  
Activity Screening

Abstract Remarkable progress in the field of antibacterial herbal therapy has been made in recent decades in response to the development of drug-resistant pathogens in aquaculture. Studies have focused on the in vitro antimicrobial activity screening of ethanolic extracts of various plants belonging to the genus Ficus. The aim of the present study was to evaluate the antibacterial efficacy of ethanolic extracts of various Ficus species against Yersinia ruckeri. In vitro tests for antibacterial activity revealed that ethanolic leaf extracts of various Ficus species and their cultivars offer a promising alternative to antibiotics and chemotherapeutics for controlling the growth of Y. ruckeri. In our study, ethanolic extracts obtained from leaves of F. natalensis subsp. leprieurii and F. macrophylla proved effective against a bacterial strain at a dose of 400 pl standardized inoculum (108 CFU ml−1). It should be noted that Y. ruckeri demonstrated an intermediate susceptibility to more extracts derived from the leaves of Ficus species. Our investigation showed that among the various Ficus species, ethanolic leaf extracts of ten Ficus species against Y. ruckeri were the most effective. The effect of the leaf extracts that expressed the highest antimicrobial activity (F. macrophylla, F. natalensis subsp. leprieurii) against Y. ruckeri was comparable to that of gentamicin. Therefore, preliminary screening indicated that the ethanolic leaf extracts of some Ficus species with antibacterial properties can be used in aquaculture as therapeutic and prophylactic agents against fish pathogens, including Y. ruckeri.

scholarly journals In Vitro Anti-Microbial Activity of Essential Oils and other Extracts from Salvia officinalis against Some Bacteria

2019 ◽  
Author(s):  
Amna Yousif Mohamed ◽  
Ahmed Ali Mustafa
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Salvia Officinalis ◽  
Diffusion Test ◽  
Hexane Extraction ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Disc

This study aimed to screen the antibacterial activity of essential oils from different parts (leave and stem) of Salvia officinalis against some Gram positive and Gram negative bacteria using agar disc diffusion test, then the extracts were prepared by hydro distillation to extract the essential oils. Maceration and hexane extraction by Soxhlet were used to obtain crude extracts from the leave and stem. Essential oils from the leaves and the ethyl acetate extract of the leaves showed higher antimicrobial activity, while hexane extract of leaves and stems showed moderate antibacterial activity. In contrast the essential oil from the stems showed very low antibacterial activity. It was observed that the results gram positive bacteria (staphylococcus aureus) was more sensitive than Gram negative (Echerichia coli).

scholarly journals Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Stefania M. Marotta ◽  
Filippo Giarratana ◽  
Alessio Parco ◽  
Domenico Neri ◽  
Graziella Ziino ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Listeria Monocytogenes ◽  
Essential Oils ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Disc Diffusion Method ◽  
Strong Activity ◽  
Agar Disc ◽  
Inhibition Experiment

Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight <em>Listeria monocytogenes</em> strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for <em>L. monocytogenes</em> strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P&lt;0.01), rather than the BEO employed (P&gt;0.01). In conclusion, we can consider BEO as a natural technological hurdle for <em>Listeria monocytogenes</em> in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

scholarly journals Antimicrobial Activity of Undecan-x-Ones (x = 2-4)

2010 ◽  
Vol 59 (4) ◽  
pp. 301-306 ◽  
Author(s):  
ALINA KUNICKA-STYCZYŃSKA ◽  
JULIA GIBKA
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Population Viability ◽  
Gram Negative Bacteria ◽  
Disc Diffusion ◽  
Gram Negative ◽  
Fungistatic Activity ◽  
Agar Disc ◽  
Microbial Strains

As a continuation of our research on the biological activity of undecan-x-ones (x = 2-4), their antimicrobial activity towards bacteria Escherichia coli and Bacillus subtilis, yeast Candida mycoderma and mould Aspergillus niger, was investigated. The population viability of the tested microbial strains in the presence of undecan-x-ones was determined by the impedimetric and agar disc diffusion methods. Undecan-x-ones showed low antibacterial activity towards both Gram-positive and Gram-negative bacteria. Undecan-2-one and undecan-3-one exhibited high activity towards C. mycoderma. All undecan-x-ones expressed the strongest effect on A. niger. The tests have proven that due to high fungistatic activity undecan-x-ones can be used to aid stabilization of food and cosmetic matrices.

scholarly journals In vitro Antibacterial Activity of Mentha Essential Oils Against Staphylococcus aureus

2017 ◽  
Vol 61 (3) ◽  
pp. 71-77 ◽  
Author(s):  
P. Horváth ◽  
J. Koščová
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Mentha Piperita ◽  
Disc Diffusion ◽  
Mentha Spicata ◽  
Methicillin Resistant ◽  
Agar Disc ◽  
Spearmint Oil

AbstractPlant extracts and essential oils (EOs) are characterized by their antibacterial properties against various bacterial pathogens, including staphylococci. Some strains of these bacteria are resistant against the adverse effects of the environment including antibiotics, e. g. methicillin-resistantStaphylococcus aureus(MRSA). EOs alone cannot substitute for antibiotics but their treatment may be useful to intensify and strengthen the effects of antibiotics on pathogenic staphylococci. In this work, we tested the antibacterial effects of the essential oils ofMenthaspecies with menthol as one of the effective substances against different strains ofS. aureus. Twoin vitromethods were used, the qualitative disc diffusion assay and the quantitative minimal inhibitory concentration (MIC) of selected essential oils. Peppermint oil fromMentha piperita, spearmint oil fromMentha spicatavar.crispaand cornmint oil fromMentha arvensiswere tested in this study against the various strains ofStaphylococcus aureus, including methicillin resistantStaphylococcus aureus(MRSA). The oils were dissolved in DMSO (dimethyl sulfoxide) and diluted at the following ratios: 1 : 1, 1 : 2, 1 : 5, and 1 : 10. Based on the results determined by the agar disc diffusion test, the highest antibacterial properties were observed in spearmint oil againstS. aureusCCM 4223 at 1 : 2 ratio where the inhibition zone varied at a range of 35.67 ± 6.81 mm. We determined also the MIC of all the oils where concentrations of the oils were as follows: 1 %; 0.5 %; 0.25 %; 0.125 % and 0.0625 %. The lowest concentrations of essential oils that possessed inhibitory effects on the growth ofS. aureusvaried between 0.125 % and 0.25 %.

scholarly journals In vitro Evaluation of the Antimicrobial Potential of Salvia officinalis L. against Oral Pathogens

2019 ◽  
Vol 21 (2) ◽  
pp. 129
Author(s):  
Carolina Medeiros Almeida ◽  
Eveline Angélica Lira de Sousa Sales Rocha ◽  
Érika Ponchet Alves ◽  
Rennaly De Freitas Lima ◽  
Yuri Wanderley Cavalcanti ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Candida Glabrata ◽  
Candida Krusei ◽  
Salvia Officinalis ◽  
Streptococcus Salivarius ◽  
Streptococcus Sanguis ◽  
Antimicrobial Potential ◽  
Streptococcus Oralis ◽  
Salvia Officinalis L

AbstractThe emergence of multidrug-resistant strains to antibiotics has motivated the search for new substances with antimicrobial activity, especially those derived from medicinal plants. Salvia officinalis L. is a medicinal plant that arouses scientific interest due to being associated with multiple therapeutic effects. The purpose of this study was to evaluate the in vitro antimicrobial potential of S. officinalis L. against pathogens in the oral cavity. The antimicrobial potential of the ethanol extract of leaf of S. officinalis L was evaluated by broth microdilution, with determination of minimum inhibitory concentration (MIC) and Minimum bactericidal/Fungicide concentration (MBC / MFC), against the species Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei and Candida tropicalis. The extract showed moderate antifungal potential before Candida species (MIC = 1 mg/mL). And for the species of Streptococcus, the antimicrobial activity was from moderate to strong whose MIC ranged from 0.25 to 1 mg/mL. In this study, the extract from the leaves of S. officinalis L. presented oral cavity antimicrobial activity against pathogens. These results point to S. officinalis as a possible source of active ingredients in the development of formulations with antimicrobial activity of dental use. Keywords: Plants, Medicinal. Salvia officinalis L. Products with Antimicrobial action.ResumoO surgimento de cepas multirresistentes a antibióticos tem motivado a busca de novas substâncias com atividade antimicrobiana, especialmente aquelas oriundas de plantas medicinais. A Salvia officinalis L. é uma planta medicinal que desperta interesse científico por estar associada a múltiplos efeitos terapêuticos. O objetivo do presente estudo foi avaliar o potencial antimicrobiano in vitro da S. officinalis L. frente a patógenos da cavidade bucal. O potencial antimicrobiano do extrato etanólico da folha da S. officinalis L foi avaliado por meio da microdiluição em caldo, com determinação da Concentração Inibitória Mínima (CIM) e Concentração Bactericida/Fungicida Mínima (CBM/CFM), frente as espécies Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, Streptococcus salivarius, Streptococcus sanguis, Candida albicans, Candida glabrata, Candida guillermond, Candida krusei e Candida tropicalis. O extrato apresentou moderado potencial antifúngico frente às espécies de Candida (CIM = 1 mg/mL). Para as espécies de Streptococcus, o potencial antimicrobiano foi considerado forte a moderado, com valores de CIM variando entre 0,25 a 1 mg/mL. Neste estudo, o extrato da folha de S. officinalis L. apresentou potencial antimicrobiano contra patógenos da cavidade bucal. Esses resultados apontam a S. officinalis como uma possível fonte de princípios ativos no desenvolvimento de formulações com atividade antimicrobiana de uso odontológico. Palavras-chave: Plantas Medicinais. Salvia officinalis L. Produtos com Ação Antimicrobiana.

scholarly journals Phytochemical profiling, antioxidant potentiality and antibacterial activity of the ethanolic extracts of Rosenvingea sp. of Bay of Bengal

2021 ◽  
Vol 45 (1) ◽  
pp. 59-71
Author(s):  
Md Abdul Alim ◽  
Md Farzanoor Rahman ◽  
Tamim Ahsan ◽  
Toufiqul Islam ◽  
Md Morshedul Alam ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bay Of Bengal ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Agar Disc

The objective of the present study was to explore the phytochemical profiling, antioxidant and antimicrobial activity of 50% ethanolic extract of Rosenvingea sp. found in the Bay of Bengal of Bangladesh. Seven phytochemicals were tested from the ethanolic extract of Rosenvingea, where four phytochemicals, namely steroid, glycosides, alkaloids, and tannins were present. However, ethanol extract exhibited low antioxidant activity compared to standard ascorbic acid as measured by DPPH-Free radical scavenging assay. In Brine Shrimp lethality bioassay, 50% ethanolic extract showed an LC50 value of 10.88 mg/mL, whereas positive control (K2Cr2O7) showed LC50 59.97 µg/mL suggesting the less toxic property of the ethanolic extract. In-vitro antimicrobial activity of ethanolic extract of Rosenvingea sp. was investigated against gram-positive and gram-negative bacteria species (Staphylococcus, Bacillus, Pseudomonas, Salmonella, and Klebsiella) by agar disc diffusion method. The highest antibacterial activity was noticed against Klebsiella pneumonia, Pseudomonas aeruginosa, Staphylococcus hominis with a zone of inhibition of 5.00±1.00 mm, 3.66±0.57 mm, and 3.33±0.57 mm, respectively. This is the first study on Rosenvingea sp. from the Bay of Bengal, reporting its phytochemical, antioxidant, and antimicrobial potentiality. However, more study is required to elucidate its commercial viability in the food and medicine industries. J. Bangladesh Acad. Sci. 45(1); 59-71: June 2021

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