scholarly journals Evaluation of the antibacterial activity of bergamot essential oils on different Listeria monocytogenes strains

2016 ◽  
Vol 5 (4) ◽  
Author(s):  
Stefania M. Marotta ◽  
Filippo Giarratana ◽  
Alessio Parco ◽  
Domenico Neri ◽  
Graziella Ziino ◽  
...  

Essential oils are aromatic and volatile substances extracted from plants and characterized by antimicrobial activity. The aim of the present study was to evaluate the antibacterial activity (agar disc-diffusion method) of seven different bergamot essential oils (BEOs) on eight <em>Listeria monocytogenes</em> strains. Minimal inhibitory concentration (MIC) of most efficient BEOs was estimated. Extremely variable results for agar disc-diffusion method for <em>L. monocytogenes</em> strains were reported. One of the tested microorganisms resulted insensible to all the BEOs; 3 strains showed an inhibition from weak to null and the remaining 4 a variable susceptibility. Among the BEOs tested, one showed a strong activity against four pathogenic strains. Four BEOs revealed weak, moderate or null activity in all the 7 sensitive strains, while for two oils only a weak or no activity was reported. MIC values were 0.625 μL/mL for the most efficient BEO, 2.5 and 5 μL/mL for the other samples that showed moderate inhibition. Experiment results are significantly related to the strains tested (P&lt;0.01), rather than the BEO employed (P&gt;0.01). In conclusion, we can consider BEO as a natural technological hurdle for <em>Listeria monocytogenes</em> in combination with other preservation strategies. Finally, this study underlines the necessity to evaluate the antimicrobial activity of EOs on a significant strains number of the same bacteria.

2007 ◽  
Vol 2 (5) ◽  
pp. 1934578X0700200
Author(s):  
Fadwa El Hanbali ◽  
Ahmed El Hakmaoui ◽  
Fouad Mellouki ◽  
Lahoussine El Rhaffari ◽  
Mohamed Akssira

The essential oil of the aerial parts of Anvillea radiata Coss. & Dur. was examined by GC-MS. Twenty-nine constituents were identified, representing 88.8% of the total. 6-Oxocyclonerolidol (66.6%) and 6-hydroxycyclonerolidol (11.4%) were found to be the major components. The disc diffusion method, used for antimicrobial activity testing, showed that the essential oil had a significant antibacterial activity against all tested microorganisms.


2015 ◽  
Vol 34 ◽  
pp. 15-20 ◽  
Author(s):  
S. Mahendran ◽  
D. Kumarasamy

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.


2021 ◽  
Vol 5 (1) ◽  
pp. 1-11
Author(s):  
Miroslava Kačániová ◽  
Alīna Klūga ◽  
Margarita Terentjeva ◽  
Simona Kunová ◽  
Katarína Rovná ◽  
...  

Abstract The aim of study was to determine the antimicrobial activity of ten essential oils against ten microorganisms isolated from fish. The following essential oils were tested: Cymbopogon nardus Rendle, Citrus limon, Leptospermum petersonii Bailey, Gaultheria procumbens, Pelargonium graveolens, Citrus paradisi, Citrus aurantifolia, Litsea cubeba Pers., Citrus reticulata Blanko, Vetiveria zizanoides. The disc diffusion method and microdilution method were used for determination of antimicrobial activity. Rhodotorula spp., Aerococcus spp., Aerococcus viridans, Enterococcus faecium, Enterococcus moraviensis, Aeromonas spp., Delftia acidovorans, Pseudomonas gessardii, Yersinia spp., Yersinia ruckeri, Yersinia enterocolitica were tested in the present study. The best antimicrobial activity of Leptospermum petersonii Bailey were found against Aerococcus spp., Enterococcus faecium, E. moraviensis, Delftia acidovorans, Pseudomonas gessardii, Yersinia ruckeri and Y. enterocolitica. Litsea cubeba Pers. was effective against Delftia acidovorans, Yersinia enterocolitica, Enterococcus faecium using the disc diffusion method. The best minimal inhibition concentration (MIC 50 - 1.5 µL/mL resp. MIC 90 - 1.6 µL/mL) was found for Litsea cubeba Pers. against Aerococcus spp. and Yersinia spp. using microdilution method.


1970 ◽  
Vol 7 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Mohammad S Rahman ◽  
Mohammed Z Rahman ◽  
Md Abdul Wahab ◽  
Rasheduzzaman Chowdhury ◽  
Mohammad A Rashid

The antimicrobial activity of methanol extracts of 17 plant species of Bangladesh was evaluated by the agar disc diffusion method. Among those, eight plant extracts exhibited potent antimicrobial activity against a wide variety of Gram positive and Gram negative bacteria and fungi at a concentration of 400 μg /disc.Key words: Antimicrobial activity, Disc diffusion method.DOI = 10.3329/dujps.v7i1.1213Dhaka Univ. J. Pharm. Sci. 7(1): 23-26, 2008 (June)


2017 ◽  
Vol 58 (4) ◽  
Author(s):  
Anne-Laure Chauvin ◽  
Crecencio Rodríguez Flores ◽  
Alizé Pennec ◽  
Caroline Nugier-Chauvin ◽  
Richard Daniellou ◽  
...  

<p>Samples of lavender, rosemary and laurel essential oils obtained from plants cultivated in Mexico were analysed by GC-MS using a non polar capillary column. Characterisation of individual components was performed using a commercial mass spectrometry library, and 53 components were identified. The analysis of bioactive compounds highlighted a 5 and 2-fold increased quantity of potent antibacterial 1,8-cineole (46.5%) and camphor (12.3%) in lavandin “provence”, compared to the French cultivars. It also showed a 3- and 2-fold increase in camphor and linalool concentration in rosemary and laurel compared to the average level, making them promising alternatives for medicinal treatment. Strains of Gram-positive and Gram-negative bacteria were analyzed for their susceptibility to these essential oils using the agar disc diffusion method and their antimicrobial activity was demonstrated. To our knowledge, this is the first report on antibacterial activity of lavender essential oil, recently added to the Mexican pharmacopeia, against <em>Mycobacterium smegmatis</em><em> </em>(Mc Rv 3782 PW2).</p>


Author(s):  
Nurliana Nurliana ◽  
Mirnawati Sudarwanto ◽  
Lisdar I. Sudirman ◽  
A. W. Sanjaya

This initial research was intended to detect antibacterial activity of pliek u oil and pliek u. Pliek u oil consist of minyeuk simplah (MS) and minyeuk brok (MB), Pliek u consist of two kinds of solid waste namely wet pliek u (Ap1) and dry pliek u (Ap2). Pliek u was methanol extracted at concentration 10% (w/v). Pliek u oil and methanol extract of pliek u were evaluated for their antibacterial activity, against Bacillus subtilis and four strains of Enterophatogenic Escherichia coli (EPEC) employing agar disc diffusion method. No antibacterial activity was shown by MS. The MB exhibited a little effect 1-2 mm against bacterial tests. The results demonstrated that the higher antibacterial activity was shown by dry pliek u compare with wet pliek u with the inhibition zones from 6.67-10.33 mm and 6.00-7.33 mm respectively.


2015 ◽  
Vol 2 (1) ◽  
pp. 11-14
Author(s):  
Elayaperumal R ◽  
Kiruthika M ◽  
Dharmalingam P

A mononuclear copper(II) complex of 1-(1H-benzimidazol-2-yl)-N-(tetrahydrofuran-2- ylmethyl)methanamine) was synthesized and characterized by various physicochemical techniques like cyclic voltammetry and elemental analysis, ESI-MS, UV–Visible, Infra red and EPR spectroscopy. The antibacterial activities of the ligand and its complex were screened by disc diffusion method and found that the metal complexes have higher antimicrobial activity than the free ligand.


2016 ◽  
Vol 3 (2) ◽  
pp. 89-93
Author(s):  
Deepthi M.P ◽  
Kathireswari P

Earthworms are the first annulated coelomate organism with haemoglobin in the plasma as carrier of oxygen and carbon dioxide. They have the antimicrobial activity against the disease causing micro organisms. In the present study antibacterial activity of earthworm coelomic fluid Lampito mauritii were confinedagainst disease causing micro organisms through the inhibition zone formation by well diffusion method as well as disc diffusion method.


2012 ◽  
pp. 239-246
Author(s):  
Gordana Dimic ◽  
Suncica Kocic-Tanackov ◽  
Olivera Jovanov ◽  
Dragoljub Cvetkovic ◽  
Sinisa Markov ◽  
...  

Commercial extracts of three spices (lemon, caraway and basil) against Listeria monocytogenes, L. innocua and L. welshimeri were investigated using disc diffusion method. Lemon and basil extracts inhibited all the organisms at the level of ? 5 ?l (lemon) and 20 ?l (basil). These extracts produced inhibitory zones of 9-19 mm (lemon) and 8-11.5 mm (basil). The extract of caraway showed activity only against L. innocua at the highest level (20 ?l), producing an inhibitory zone of 14.7 mm. Generally, the lemon extract was the most effective. This extract exhibited greater inhibitory activity against L. monocytogenes, while the basil extract had the strongest effect on L. welshimeri.


2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Somayeh Razmavar ◽  
Mahmood Ameen Abdulla ◽  
Salmah Binti Ismail ◽  
Pouya Hassandarvish

This study was based on screening antibacterial activity of the ethanol extract ofBaeckea frutescensL. against MRSA clinical isolates, analyzes the potential antibacterial compound, and assesses the cytotoxicity effect of the extract in tissue culture. Leaves ofBaeckea frutescensL. were shade dried, powdered, and extracted using solvent ethanol. Preliminary phytochemical screening of the crude extracts revealed the presence of alkaloids, flavonoids, steroids, terpenoids, phenols, and carbohydrates. The presence of these bioactive constituents is related to the antibacterial activity of the plant. Disc diffusion method revealed a high degree of activity against microorganisms. The results confirm thatBaeckea frutescensL. can be used as a source of drugs to fight infections caused by susceptible bacteria.


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