scholarly journals Biotransformation of Indigo Pigment by Indigenously Isolated Pseudomonas sp. HAV-1 and Assessment of Its Antioxidant Property

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Aditi Dua ◽  
Kishor Chauhan ◽  
Hilor Pathak
Keyword(s):  
Ascorbic Acid ◽  
Therapeutic Potential ◽  
Growth Parameters ◽  
Analytical Techniques ◽  
Antioxidant Property ◽  
Pseudomonas Sp ◽  
Environment Friendly ◽  
Green Production ◽  
Indigo Production ◽  
Human Health Effects

Chemical synthesis of indigo poses harsh environmental hazards and adverse human health effects. This necessitates an environment-friendly and producer-friendly approach for indigo production. The present study was thus significant as it reports an indigenously isolated potential indigo pigment producing culture identified as Pseudomonas sp. HAV-1 with noteworthy antioxidant property. The bioindigo pigment was characterized using various analytical techniques. The pigment production was enhanced from 412 μg mL−1 to 700 μg mL−1 by optimizing the growth parameters. Furthermore, the antioxidant property of indigo pigment is hitherto unexplored. This property can significantly append to its therapeutic potential. The bioindigo pigment produced by Pseudomonas sp. HAV-1 depicted 2.2 μM ascorbic acid equivalent antioxidant property. More to the point, the present work addresses a footstep towards green production of indigo.

Interaction of Tomatoes (Solanum lycopersicum L.) Transformed by rapA1 Gene with Pseudomonas sp. 102 Bacteria Resistant to High Cadmium Concentrations as a Basis for Effective Symbiotic Phytoremediation System

2019 ◽  
pp. 38-48
Author(s):  
L.R. Khakimova ◽  
A.M. Lavina ◽  
L.R. Karimova ◽  
V.V. Fedyaev ◽  
An.Kh. Baymiev ◽  
...  
Keyword(s):  
Growth Parameters ◽  
Basic Research ◽  
Toxic Effects ◽  
Research Centre ◽  
Pseudomonas Sp ◽  
Tomato Plants ◽  
Solanum Lycopersicum L ◽  
Plant Growth Promoting ◽  
High Concentrations ◽  
The Government

A Pseudomonas sp. 102 strain, which is highly resistant to toxic effects of cadmium and has plant growth-promoting activity, can significantly increase growth parameters and biomass of tomato plants, including those observed under toxic effects of cadmium. The greatest positive effect was observed in plants transformed with the bacterial adhesin gene rapA1, the product of which is important for colonization of plant roots by bacteria. It was also shown that shoots of transgenic tomato plants accumulated the greatest amount of cadmium during inoculation with Pseudomonas sp. 102. The ability to extract high concentrations of cadmium and accumulate a large biomass under stress opens up prospects for the further use of associative interactions between tomato and Pseudomonas for phytoremediation. phytoremediation, cadmium, tomato, Pseudomonas, inoculation, agglutinins, This study was carried out using the equipment of the Biomika Centre for Collective Use of the Institute of Biochemistry and Genetics (Ufa Federal Research Centre, Russian Academy of Sciences) as part of the government task (project no. AAAA-A16-1160203500284). This study was supported by the Russian Foundation for Basic Research (project nos. 18-34-20004 and 18-34-00033) and 18-344-0033 mol_a_ved and 34-00033 mol_a).

scholarly journals Ferulic Acid: Therapeutic Potential Through Its Antioxidant Property

2007 ◽  
Vol 40 (2) ◽  
pp. 92-100 ◽  
Author(s):  
Marimuthu Srinivasan ◽  
Adluri R. Sudheer ◽  
Venugopal P. Menon
Keyword(s):  
Ferulic Acid ◽  
Therapeutic Potential ◽  
Antioxidant Property

scholarly journals Fertilization-Induced Changes in Growth Parameters and Antioxidant Activity of Medicinal Plants Used in Traditional Arab Medicine

2005 ◽  
Vol 2 (4) ◽  
pp. 549-556 ◽  
Author(s):  
Hassan Azaizeh ◽  
Predrag Ljubuncic ◽  
Irina Portnaya ◽  
Omar Said ◽  
Uri Cogan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Therapeutic Potential ◽  
Growth Parameters ◽  
Tap Water ◽  
Water Soluble ◽  
Wild Plants ◽  
Hoagland Solution ◽  
Β Carotene ◽  
Significant Concentration

In response to increased popularity and greater demand for medicinal plants, a number of conservation groups are recommending that wild medicinal plants be brought into cultivation systems. We collected four medicinal herbsCichorium pumilum, Eryngium creticum,Pistacia palaestinaandTeucrium poliumused in traditional Arab medicine for greenhouse cultivation to assess the effects of different fertilization regimes on their growth and antioxidant activity. Wild seedlings were collected and fertilized with either 100% Hoagland solution, 50% Hoagland solution, 20% Hoagland solution or irrigated with tap water. Plant height was measured and the number of green leaves and branches counted weekly. Thereafter, the aboveground parts of plants were harvested for preparing a water-soluble powder extracts of which antioxidant activity was measured by their ability to suppress the oxidation of β-carotene. Of the fertilization regimes, we found either 20 or 50% Hoagland solution produced the most consistent response of the plant growth parameters. All powders prepared from the four wild growing plants inhibited oxidation of β-carotene. Increasing the amount of fertilizer caused a significant concentration-dependent increase in antioxidant activity of the cultivatedT. poliumcompared with the wild type. In contrast, increasing the amount of fertilizer caused a significant concentration-dependent reduction in the antioxidant activity of powders prepared from the cultivatedE. creticumwhen compared with wild plants. Our results showed that cultivation success should not rely solely on parameters of growth but should incorporate assessment related to indices of therapeutic potential.

Indigo production by Pseudomonas sp. J26, a marine naphthalene-degrading strain

2010 ◽  
Vol 50 (3) ◽  
pp. 290-293 ◽  
Author(s):  
Juan Pablo Riva Mercadal ◽  
Paula Isaac ◽  
Faustino Siñeriz ◽  
Marcela Alejandra Ferrero
Keyword(s):  
Pseudomonas Sp ◽  
Indigo Production

scholarly journals Effect of Drought Stress on Chlorophyll Fluorescence Parameters, Phytochemical Contents, and Antioxidant Activities in Lettuce Seedlings

Horticulturae ◽  
2021 ◽  
Vol 7 (8) ◽  
pp. 238
Author(s):  
Yu Kyeong Shin ◽  
Shiva Ram Bhandari ◽  
Jung Su Jo ◽  
Jae Woo Song ◽  
Jun Gu Lee
Keyword(s):  
Ascorbic Acid ◽  
Chlorophyll Fluorescence ◽  
Drought Stress ◽  
Quantum Yield ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Photochemical Quenching ◽  
Total Phenol Content ◽  
Effective Quantum Yield ◽  
Chlorophyll Fluorescence Parameters

This study monitored changes in chlorophyll fluorescence (CF), growth parameters, soil moisture content, phytochemical content (proline, ascorbic acid, chlorophyll, total phenol content (TPC), and total flavonoid content (TFC)), and antioxidant activities in 12-day-old lettuce (Lactuca sativa L.) seedlings grown under drought stress (no irrigation) and control (well irrigated) treatments in controlled conditions for eight days. Measurements occurred at two-day intervals. Among ten CF parameters studied, effective quantum yield of photochemical energy conversion in PSII (Y(PSII)), coefficient of photochemical quenching (qP), and coefficient of photochemical quenching of variable fluorescence based on the lake model of PSII (qL) significantly decreased in drought-stressed seedlings from day 6 of treatment compared to control. In contrast, maximum quantum yield (Fv/Fm), ratio of fluorescence (Rfd), and quantum yield of non-regulated energy dissipation in PSII (Y(NO)) were significantly affected only at the end. All growth parameters decreased in drought-stressed seedlings compared to control. Proline started increasing from day 4 and showed ~660-fold elevation on day 8 compared to control. Chlorophyll, ascorbic acid, TPC, TFC, and antioxidant activities decreased in drought-stressed seedlings. Results showed major changes in all parameters in seedlings under prolonged drought stress. These findings clarify effects of drought stress in lettuce seedlings during progressive drought exposure and will be useful in the seedling industry.

scholarly journals Exploration of Potential Bacterial Endophytes against Root Knot Nematode, Meloidogyne incognita in Banana

2021 ◽  
Vol 108 (Special) ◽  
Author(s):  
Ganeshan k ◽  
◽  
Vetrivelkalai p ◽  
Bhagawati B ◽  
Nibha G ◽  
...  
Keyword(s):  
Meloidogyne Incognita ◽  
Biochemical Characterization ◽  
Growth Parameters ◽  
Pseudomonas Sp ◽  
Bacterial Endophytes ◽  
Bacterial Isolates ◽  
Root Knot Nematode ◽  
Egg Hatching ◽  
Juvenile Mortality ◽  
Pot Culture

A field survey was conducted in 12 districts of Assam viz., Jorhat, Golaghat, Nagaon, Marigaon, Goalpara, Dibrugarh, Tinsukia, Lakhimpur, Dhemaji, Sivsagar, Kamrup and Barpeta. A total of 92 root samples were collected and 37 bacterial isolates were isolated from commercial banana cultivars. The culture filtrates extracted from 37 endophytic bacterial isolates, were screened against southern root-knot nematode, Meloidogyne incognita in vitro and under pot culture studies. The five bacterial isolates viz., EB4, EB8, BC1, BC11 and BC12 showed 100% inhibition of egg hatching and juvenile mortality of M. incognita with an exposure period of 48 and 72h. On seed bacterization, with these five promising isolates, two isolates viz.EB4, BC1 significantly enhanced germination percentage (33.33, 25.31%) and vigour index (75.5, 64.39%) of paddy, receptively. The potential bacterial isolates viz., BC1 and EB4 were identified as Lysinibacillus sp. and Pseudomonas sp., respectively , based on the morphological phenotypic and biochemical characterization. The pot culture experiment revealed that the bacterial endophytes viz., Lysinibacillus sp. (BC1) Pseudomonas sp. (EB4) significantly reduced the soil (61.64, 56.71%) and root nematode population (77.29, 68.87%), number of adult females (73.97, 69.89%), egg masses (85.63, 80.11%) and root-knot index (1.33, 1.67) of M. incognita compared to untreated control. The bacterial endophytes viz., Pseudomonas sp. (EB4), Lysinibacillus sp. (BC1) were also significantly increased the growth parameters viz., shoot length (43.33, 39.18%), and root length (78.24, 59.26%) and pesudostem girth (58.38, 52.13%).

Synthesis and Evaluation of 198Au/PAMAM-MPEG-FA against Cancer Cells

2020 ◽  
Vol 20 (10) ◽  
pp. 1250-1265
Author(s):  
Reza Rezaei ◽  
Simin Janitabar Darzi ◽  
Mahnaz Yazdani
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Therapeutic Potential ◽  
Analytical Techniques ◽  
Cell Uptake ◽  
Breast Adenocarcinoma ◽  
Human Breast ◽  
Clinical Investigations ◽  
Anti Cancer

Background: There is a significant dearth of clinical biochemistry researches to evaluate the facility of exploitation of folate targeted radioactive gold-labeled anti-cancer drugs against various cancer cell lines. Objective: The aim of this paper was to develop a gold-based compound with an efficient therapeutic potential against breast cancer. To this end, the synthesis of the 198Au/PAMAM-MPEG-FA composite was considered here. Methods: The radioactive gold (198Au) nanoparticles were encapsulated into Folic acid (FA)-targeted Polyamidoamine dendrimer (PAMAM) modified with Maleimide-Polyethylene glycol Succinimidyl Carboxymethyl ester (MPEG). After that, anticancer assessments of the prepared 198Au/PAMAM-MPEG-FA hybrid mater against breast cancer were investigated. : Further studies were also devised to compare the anticancer capabilities of the 198Au/PAMAM-MPEG-FA composite with the synthesized P-MPEG, 197Au/P-MPEG, 197Au/P-MPEG-FA, 197Au/P-FA and 198Au/P-MPEG-FA conjugates. The prepared drugs were characterized by means of various analytical techniques. The radionuclidic purity of the 198Au/P-MPEG-FA solution was determined using High Purity Germanium (HPGe) spectroscopy and its stability in the presence of human serum was studied. The cell uptake and toxicity of the prepared drugs were evaluated in vitro, and some comparative studies of the toxicity of the drugs were conducted towards the MCF7 (Human breast cancer cell), 4T1 (Mice breast adenocarcinoma cell) and C2C12 (Mice muscle normal cell). Results: The results showed that cell uptake of 198Au/P-MPEG-FA nanoparticles is high in the 4T1 cell line and the order of uptake is as 4T1> MCF7> C2C12. Moreover, of the tested compounds, 198Au/P-MPEG-FA had the highest toxicity towards the cancerous 4T1 and MCF7 in all concentrations after 24, 48 and 72h (P < 0.001). Furthermore, the cytotoxicity of the drugs was concentration-dependent. Conclusion: On the basis of the present research, 198Au/P-MPEG-FA has been proposed as a good candidate for the induction of cell death in breast cancer, although further experimental and clinical investigations are required.

scholarly journals Antioxidant Effects of Protocatechuic Acid and Protocatechuic Aldehyde: Old Wine in a New Bottle

2021 ◽  
Vol 2021 ◽  
pp. 1-19
Author(s):  
Shijun Zhang ◽  
Zhibo Gai ◽  
Ting Gui ◽  
Juanli Chen ◽  
Qingfa Chen ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Protocatechuic Acid ◽  
Therapeutic Potential ◽  
Antioxidant Property ◽  
Biological Properties ◽  
Structural Variations ◽  
Beneficial Effects ◽  
Protocatechuic Aldehyde

Phenolic compounds are naturally present as secondary metabolites in plant-based sources such as fruits, vegetables, and spices. They have received considerable attention for their antioxidant, anti-inflammatory, and anti-carcinogenic properties for protection against many chronic disorders such as neurodegenerative diseases, diabetes, cardiovascular diseases, and cancer. They are categorized into various groups based on their chemical structure and include phenolic acids, flavonoids, curcumins, tannins, and quinolones. Their structural variations contribute to their specific beneficial effects on human health. The antioxidant property of phenolic compounds protects against oxidative stress by up-regulation of endogenous antioxidants, scavenging free radicals, and anti-apoptotic activity. Protocatechuic acid (PCA; 3,4-dihydroxy benzoic acid) and protocatechuic aldehyde (PAL; 3,4-dihydroxybenzaldehyde) are naturally occurring polyphenols found in vegetables, fruits, and herbs. PCA and PAL are the primary metabolites of anthocyanins and proanthocyanidins, which have been shown to possess pharmacological actions including antioxidant activity in vitro and in vivo. This review aims to explore the therapeutic potential of PCA and PAL by comprehensively summarizing their pharmacological properties reported to date, with an emphasis on their mechanisms of action and biological properties.

scholarly journals Surfactin-Like Biosurfactant Production and Optimization by Bacillus Subtilis SNW3: Product Characterization and Its Influence on Seed Development and Plant Growth

2021 ◽  
Author(s):  
Aiman Umar ◽  
Aneeqa Zafar ◽  
Hasina Wali ◽  
Meh Para Siddique ◽  
Zulfiqar Ali Malik ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Seed Germination ◽  
Plant Growth ◽  
Seed Development ◽  
Growth Parameters ◽  
Frying Oil ◽  
Biosurfactant Production ◽  
Waste Frying Oil ◽  
Environment Friendly ◽  
Industrial Sectors

Abstract Background: Biosurfactants, being environment friendly, highly biodegradable, less toxic and stable compounds have applications in several environmental and industrial sectors that includes cosmetics, biomedical, bioremediation, and agriculture. Growing concern about eco-friendly compounds leads to replacement of chemical surfactants with biological surfactants. However, use of biosurfactant limits due to high production cost. Surfactin, a class of lipopeptide, considered as powerful biosurfactants having wide applications in therapeutics and environmental field. This study aims to investigate production and characterization of surfactin by Bacillus subtilis SNW3 and evaluating their potential application in seed germination and plant growth. Results: In present study, Bacillus subtilis SNW3 was previously isolated from Chakwal Pakistan and used for biosurfactant production. Optimized media for biosurfactant production was at (6% w/v) white beans powder in combination with (1.5% w/v) waste frying oil and (0.1% w/v) urea that shows surface tension reduction (28.8 mN/m), oil displacement assay (4.9 cm) and emulsification index (69.8 %). Environmental growth parameters like temperature (30 °C), inoculum size (1%), pH (6) and agitation (150 rpm) exhibit important role towards enhanced biosurfactant yield. Furthermore, surfactin obtained was found to be most stable at (5-7) pH, (8%) NaCl and (100 °C) temperature. Biosurfactant obtained was of lipopeptide nature called surfactin characterized by thin-layer chromatography (TLC) and fourier-transform infrared spectroscopy (FTIR). The surfactin obtained, used in a concentration of (0.7 g/100 mL) helps in seed germination and significantly enhanced growth of Solanum lycopersicum (tomato), Pisum sativum (pea), Capsicum annuum (peppers) and Lactuca sativa (lettuce).Conclusions: Bacillus subtilis SNW3 produces surfactin with more stability, that makes it useful for processing of food and in agriculture. The use of white beans powder and waste frying oil as sole source of carbon and energy makes the biosurfactant production more profitable, and environment friendly procedure by utilizing food processing by-products and wastes as substrate. Results obtained provide understanding about surfactin use for seed development and plant growth.

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