scholarly journals High-Performance Thin-Layer Chromatographic Quantification of Rosmarinic Acid and Rutin in Abnormal Savda Munziq

2013 ◽  
Vol 2013 ◽  
pp. 1-4
Author(s):  
S. G. Tian ◽  
L. D. Xin ◽  
Halmuart Upur
Keyword(s):  
Thin Layer ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Rapid Screening ◽  
Thin Layer Plate ◽  
Calibration Data ◽  
Linear Calibration ◽  
Color Reagent ◽  
Abnormal Savda ◽  
Abnormal Savda Munziq

A high-performance thin-layer chromatographic (HPTLC) method has been established for simultaneous analysis of rosmarinic acid and rutin in Abnormal Savda Munziq (ASMq). A methanol extract of ASMq was used for quantification. The compounds were separated on silica gel H thin layer plate with ethyl acetate-formic acid-acetic acid-water 15 : 1 : 1 : 1.5 (v/v) as a developer, trichloroethanol as the color reagent. The plates were scanned at 365 nm. The linear calibration data of rosmarinic acid and rutin were in the range of 0.0508 to 0.2540 μg(r=0.9964), 0.2707 to 1.35354μg(r=0.9981), respectively. The recovery rate of rosmarinic acid was 99.17% (RSD = 2.92%) and rutin was 95.24% (RSD = 2.38%). The method enables rapid screening, precise, selective, and sensitive quantification for pharmaceutical analysis.

VALIDATED STABILITY INDICATING HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC METHOD FOR REPAGLINIDE API

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (09) ◽  
pp. 48-54
Author(s):  
P. P. Thakkar ◽  
◽  
N. R Patel ◽  
C. S Kothari ◽  
R Patel ◽  
...  
Keyword(s):  
Thin Layer ◽  
High Performance ◽  
Chromatographic Method ◽  
Degradation Products ◽  
Linear Regression Analysis ◽  
Tablet Formulation ◽  
Content Uniformity ◽  
Calibration Data ◽  
Stability Indicating ◽  
Photolytic Degradation

A simple, precise and accurate stability-indicating high performance thin layer chromatographic method for estimation of repaglinide in bulk drug and in tablet formulation has been developed and validated. The stationary phase used was HPTLC plates precoated with silica gel 60F254 using the mobile phase of chloroform: methanol: ammonia (4.5:1.0:0.05 V/V/V). Densitometric analysis was performed in absorbance mode at 242 nm. The method showed compact bands of drug at RF value of 0.31 ± 0.02. Linear Regression analysis of calibration data showed good linear relationship with r2= 0.9981 in concentration range of 500 - 3000 ng/band. Drug was subjected to ICH-prescribed stress conditions such as acid, base, peroxide, thermal and photolytic degradation and method was found able to separate the peaks for all degradation products from analyte peak. Validation of the developed method was carried out for its specificity, linearity, range, precision, accuracy and robustness. The method was further applied for repaglinide estimation in pharmaceutical tablet formulation and it was found to be reliable. The method was also used successfully to carry out content uniformity test of repaglinide in tablet dosage form.

scholarly journals HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHIC DETERMINATION OF CAFFEIC ACID AND ROSMARINIC ACID FROM THE LEAVES OF Orthosiphon stamineus

2010 ◽  
Vol 9 (1) ◽  
pp. 137-141
Author(s):  
M. Amzad Hossain ◽  
Zhari Ismail
Keyword(s):  
Thin Layer ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
High Performance ◽  
Chromatographic Determination ◽  
Hplc Method ◽  
Caffeic Acid Derivatives ◽  
Orthosiphon Stamineus ◽  
Pharmaceutical Properties

This paper presents the studies performed on extraction of Orthosiphon stamineus, Benth by using different solvent for the identification and quantification of the caffeic acid derivatives such as caffeic acid  and rosmarinic acid which confers to the leaves of this plant with remarkable pharmaceutical properties. High performance thin-layer chromatographic (HPTLC) allows the identification and the quantification of more than 20 samples in the same chromatographic run. The analysis of the samples requires 15-30 min compared with more than 2 h using a typical HPLC method. Using the techniques of the HPTLC and the UV-VIS spectra we have found that the extraction of this herb plant contain, the caffeic acid and rosmarinic acid ranging between 0.029% up to 0.506% and up to 0.24% to 2.24% respectively.     Keywords: Caffice acid derivatives, quantification, Malaysian Orthosiphon stamineus, HPTLC

scholarly journals Determination of Rosmarinic Acid and Caffeic Acid in Thymus daenensis and Thymus lancifolius Extracts by High-Performance Thin Layer Chromatography

2021 ◽  
Vol 10 (4) ◽  
pp. 2804-2809
Keyword(s):  
Thin Layer ◽  
Formic Acid ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Mobile Phase ◽  
High Performance ◽  
Layer Chromatography

Thymus species belong to the Lamiaceae family, of which 18 species in the flora of Iran, 6 are endemic to Iran. In the current research, high-performance thin-layer chromatography (HPTLC) technique as an easy, fast, reproducible, and low-cost method was used for the determination of rosmarinic acid and caffeic acid in Thymus lancifolius (T. lancifolius) and two species of Thymus daenensis (T. daenensis) from Iran. Toluene-ethyl acetate-formic acid with a ratio of 67.72-22.90 and 9.38% was selected as the mobile phase of rosmarinic acid, and ethyl acetate-methanol-formic acid-water with a ratio of 85-8-2 and 5% was designated as the mobile phase of caffeic acid. The highest and lowest amount of rosmarinic acid was observed for T. daenensis 1 (10.54±0.12 mg/g) and T. lancifolius (0.46±0.01 mg/g), respectively. The amount of rosmarinic acid for T. daenensis 2 was obtained as 7.85±0.02 mg/g for each of the dried plants. In the following, HPTLC analysis of caffeic acid for T. daenensis 1, T. daenensis 2, and T. lancifolius was acquired amounts of 0.78±0.007, 0.13±0.007, and 0.26±0.007 mg/g for each of dried plants, respectively. Therefore, regarding the special effects of phenolic acids and properties of the Thymus genus, the acquired results are suitable for application in pathogenic research, infections, immunology diseases, and evaluation of the antioxidant activity.

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